Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates DOI Creative Commons
Andrey Shelenkov,

Yulia Mikhaylova,

Shushanik Voskanyan

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1314 - 1314

Published: May 17, 2023

Klebsiella pneumoniae, a member of the Enterobacteriaceae family, has become dangerous pathogen accountable for large fraction various infectious diseases in both clinical and community settings. In general, K. pneumoniae population been divided into so-called classical (cKp) hypervirulent (hvKp) lineages. The former, usually developing hospitals, can rapidly acquire resistance to wide spectrum antimicrobial drugs, while latter is associated with more aggressive but less resistant infections, mostly healthy humans. However, growing number reports last decade have confirmed convergence these two distinct lineages superpathogen clones possessing properties both, thus imposing significant threat public health worldwide. This process horizontal gene transfer, which plasmid conjugation plays very important role. Therefore, investigation structures ways plasmids spread within between bacterial species will provide benefits prevention measures against powerful pathogens. this work, we investigated multidrug-resistant isolates using long- short-read whole-genome sequencing, allowed us reveal fusion IncHI1B/IncFIB ST512 capable simultaneously carrying hypervirulence (iucABCD, iutA, prmpA, peg-344) determinants (armA, blaNDM-1 others), obtain insights their formation transmission mechanisms. Comprehensive phenotypic, genotypic phylogenetic analysis isolates, as well repertoire, was performed. data obtained facilitate epidemiological surveillance high-risk development strategies them.

Language: Английский

A longitudinal epidemiology study of fluoroquinolone-nonsusceptible Klebsiella pneumoniae reveals an increasing prevalence of qnrB and qnrS in Taiwan DOI Creative Commons
Pei-Yun Kuo, Wei‐Hung Lin, Sheng-Hui Tang

et al.

Journal of Infection and Public Health, Journal Year: 2024, Volume and Issue: 17(3), P. 457 - 463

Published: Jan. 9, 2024

Our objective was to investigate the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in fluoroquinolone-nonsusceptible Klebsiella pneumoniae (FQNSKP) Taiwan, 1999-2022.

Language: Английский

Citations

3
Convergence and global epidemiology ofKlebsiella pneumoniaeplasmids harbouring theiuc3 virulence locus DOI Creative Commons
Marjorie J. Gibbon, Natacha Couto,

Keira Cozens

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 5, 2024

Abstract Background Klebsiella pneumoniae (Kp) is an important pathogen of humans and animals, recent reports ‘convergent’ strains that carry both virulence antimicrobial resistance genes (ARGs) have raised serious public health concern. The plasmid-borne iuc locus, encoding the siderophore aerobactin, a key factor in this species. variant 3 associated with porcine human clinical isolates carried by mostly uncharacterised IncF plasmids. Methods We used combination short-read long-read sequencing to characterise IncFIB(K)/IncFII 3-carrying plasmids harboured 79 Kp one K. oxytoca isolate recovered as part two large ‘One-Health’ studies Italy (SpARK) Thailand (OH-DART). Adding data from repositories gave combined dataset 517 isolates, were analysed using clustering phylogenetic methods. Findings note seven large, convergent, emerged through hybridisation co-circulating harbouring extended-spectrum beta-lactamases (ESBLs). also able identify putative parental which neighbouring meat markets, hybrid Clustering global analyses resolved plasmid sub-group circulating throughout Asia, occasional examples Europe elsewhere. This carries multiple ARGs commonly thus warranting targeted surveillance. Interpretation Our study reveals leading convergence traits may be very common, even non-clinical (‘One-Health’) settings. Population-scale genomics makes it possible plasmids, will help types are most likely hybridise, what selective consequences for host. A distinct sub-variant Asia requires close monitoring. Research In Context Multiple clones combine hypervirulence multidrug (MDR-hvKp) been published recently; PubMed search November 2023 words ‘convergence ’ returned 143 papers, 99 2020 onwards. demonstrates carrying AMR frequent, ongoing, process natural populations. subsequent transfer conferring driver behind spread convergent strains. provides exemplar how assemblies can facilitate large-scale genomic epidemiology. Evidence before Although highlight emergence strains, confluence within same has not studied at population level, rarely identified. Moreover, there few high-resolution epidemiology on closely related long scale. Added value more than double number complete sequences available 58 139 provide evidence host lineages harbour these (e.g., ST35), epidemiological source pig, wild animal, human). comparative analysis approaches inform future studies. Implications occurs frequently populations, However, drivers (if any) evolutionary phenomenon unclear. There clear utility generating closed genomes scale, surveillance warranted.

Language: Английский

Citations

3
Antibiotic resistance rates in hypervirulent Klebsiella pneumoniae strains: a systematic review and meta-analysis DOI Creative Commons
Masoumeh Beig, Shadi Aghamohammad,

Nahal Majidzadeh

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2024, Volume and Issue: 38, P. 376 - 388

Published: July 26, 2024

In response to the growing global concerns regarding antibiotic resistance, we conducted a meta-analysis assess prevalence of resistance in hypervirulent Klebsiella pneumoniae (hvKp) strains.

Language: Английский

Citations

3
Genomic Characterization of International High-Risk Clone ST410 Escherichia coli Co-Harboring ESBL-Encoding Genes and blaNDM-5 on IncFIA/IncFIB/IncFII/IncQ1 Multireplicon Plasmid and Carrying a Chromosome-Borne blaCMY-2 from Egypt DOI Creative Commons
Nelly M. Mohamed, Azza S. Zakaria, Eva A. Edward

et al.

Antibiotics, Journal Year: 2022, Volume and Issue: 11(8), P. 1031 - 1031

Published: July 30, 2022

The accelerated dispersion of multidrug-resistant (MDR) Escherichia coli due to the production extended-spectrum β-lactamases (ESBLs) or AmpC enzymes has been noted in Egypt, presenting a serious treatment challenge. In this study, we investigate prevalence ESBLs and among 48 E. isolates collected from patients with urinary tract infections admitted teaching hospital Alexandria. Phenotypic genotypic methods detection are conducted. Isolates producing both tested for mobilization their genes by broth mating experiment. Whole genome sequencing (WGS) is performed isolate EC13655. results indicate that 80% MDR, which 52% 13% were ESBL producers, respectively. Conjugation experiments fail show blaCMY-2 EC13655, was chosen WGS. silico analysis reveals belongs ST410-H24Rx high-risk clone. It coharbors ESBL-encoding blaCTX-M-15, blaTEM-1, blaOXA-1 blaNDM-5 on an IncFIA/IncFIB/IncFII/IncQ1 multireplicon plasmid. chromosomal location detected flanking upstream copy ISEcp1. This integration establishes stable maintenance gene thus, necessitates imperative local surveillance reduce further spread such strains different clinical settings.

Language: Английский

Citations

12
Whole-Genome Sequencing Revealed the Fusion Plasmids Capable of Transmission and Acquisition of Both Antimicrobial Resistance and Hypervirulence Determinants in Multidrug-Resistant Klebsiella pneumoniae Isolates DOI Creative Commons
Andrey Shelenkov,

Yulia Mikhaylova,

Shushanik Voskanyan

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(5), P. 1314 - 1314

Published: May 17, 2023

Klebsiella pneumoniae, a member of the Enterobacteriaceae family, has become dangerous pathogen accountable for large fraction various infectious diseases in both clinical and community settings. In general, K. pneumoniae population been divided into so-called classical (cKp) hypervirulent (hvKp) lineages. The former, usually developing hospitals, can rapidly acquire resistance to wide spectrum antimicrobial drugs, while latter is associated with more aggressive but less resistant infections, mostly healthy humans. However, growing number reports last decade have confirmed convergence these two distinct lineages superpathogen clones possessing properties both, thus imposing significant threat public health worldwide. This process horizontal gene transfer, which plasmid conjugation plays very important role. Therefore, investigation structures ways plasmids spread within between bacterial species will provide benefits prevention measures against powerful pathogens. this work, we investigated multidrug-resistant isolates using long- short-read whole-genome sequencing, allowed us reveal fusion IncHI1B/IncFIB ST512 capable simultaneously carrying hypervirulence (iucABCD, iutA, prmpA, peg-344) determinants (armA, blaNDM-1 others), obtain insights their formation transmission mechanisms. Comprehensive phenotypic, genotypic phylogenetic analysis isolates, as well repertoire, was performed. data obtained facilitate epidemiological surveillance high-risk development strategies them.

Language: Английский

Citations

7