Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

BMC Microbiology, Journal Year: 2023, Volume and Issue: 23(1)

Published: March 30, 2023

Abstract The opportunistic human pathogen Pseudomonas aeruginosa is the causal agent of a wide variety infections. This non-fermentative Gram-negative bacillus can colonize zones where skin barrier weakened, such as wounds or burns. It also causes infections urinary tract, respiratory system bloodstream. P. are common in hospitalized patients for which multidrug-resistant, respectively extensively drug-resistant isolates be strong contributor to high rate in-hospital mortality. Moreover, chronic cystic fibrosis especially concerning, since very tedious treat. exploits diverse cell-associated and secreted virulence factors, play essential roles its pathogenesis. Those factors encompass carbohydrate-binding proteins, quorum sensing that monitor production extracellular products, genes conferring extensive drug resistance, secretion deliver effectors kill competitors subvert host functions. In this article, we highlight recent advances understanding pathogenicity well efforts identification new targets development therapeutic strategies against These provide innovative promising circumvent infection caused by important pathogen.

Language: Английский

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trans-Cinnamaldehyde as a Novel Candidate to Overcome Bacterial Resistance: An Overview of In Vitro Studies

Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 254 - 254

Published: Jan. 27, 2023

The increasing of drug-resistant bacteria and the scanty availability novel effective antibacterial agents represent alarming problems modern society, which stimulated researchers to investigate strategies replace or assist synthetic antibiotics. A great deal attention has been devoted over years essential oils that contain mixtures volatile compounds have traditionally exploited as antimicrobial remedies. Among oil phytochemicals, remarkable antibiotic-potentiating activities highlighted for cinnamaldehyde, an α,β-unsaturated aldehyde, particularly abundant in Cinnamomum spp., widely used a food additive industrial products. In line with this evidence, present study, overview available literature carried out order define bacterial sensitizing profile cinnamaldehyde. vitro studies displayed ability substance resensitize microbial strains drugs increase efficacy different antibiotics, especially cefotaxime, ciprofloxacin, gentamicin; however, vivo, clinical trials are lacking. Based on collected findings, cinnamaldehyde appears be interest adjuvant agent overcome superbug infections antibiotic resistance; future more in-dept investigations should encouraged clarify its mechanisms involved.

Language: Английский

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Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2100 - 2100

Published: Feb. 8, 2024

The rising prevalence of drug-resistant bacteria underscores the need to search for innovative and nature-based solutions. One approaches may be use plants that constitute a rich source miscellaneous compounds with wide range biological properties. This review explores antimicrobial activity seven bioactives their possible molecular mechanisms action. Special attention was focused on antibacterial properties berberine, catechin, chelerythrine, cinnamaldehyde, ellagic acid, proanthocyanidin, sanguinarine against Staphylococcus aureus, Enterococcus spp., Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Serratia marcescens Pseudomonas aeruginosa. growing interest in novel therapeutic strategies based new plant-derived formulations confirmed by number articles. Natural products are one most promising intensively examined agents combat consequences overuse misuse classical antibiotics.

Language: Английский

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Quercetin: a promising virulence inhibitor of Pseudomonas aeruginosa LasB in vitro

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Jan. 5, 2024

Abstract With the inappropriate use of antibiotics, antibiotic resistance has emerged as a major dilemma for patients infected with Pseudomonas aeruginosa . Elastase B (LasB), crucial extracellular virulence factor secreted by P. , been identified key target antivirulence therapy. Quercetin, natural flavonoid, exhibits promising potential an agent. We aim to evaluate impact quercetin on LasB and elucidate underlying mechanism. Molecular docking molecular dynamics simulation revealed rather favorable intermolecular interaction between LasB. At sub-MICs ≤256 μg/ml, was found effectively inhibit production activity elastase, well downregulate transcription level lasB gene in both PAO1 clinical strains Through correlation analysis, significant positive correlations were shown QS system regulatory genes lasI lasR rhlI rhlR Then, we expression significantly deficient ΔlasI ΔlasIΔrhlI mutants. In addition, downregulated levels regulated rhlI, pqsA pqsR attenuated synthesis signaling molecules 3-oxo-C12-HSL C4-HSL PAO1. Quercetin also able compete ligands OdDHL, BHL, PQS binding receptor proteins LasR, RhlR, PqsR, respectively, resulting formation more stabilized complexes. Taken together, enormous combating disrupting vitro thereby offering alternative approach therapy infections. Key points • diminished content elastase aeruginosa. inhibited acted based system.

Language: Английский

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Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 2, 2024

Pseudomonas aeruginosa biofilm is a community of bacteria that adhere to live or non-living surfaces and are encapsulated by an extracellular polymeric substance. Unlike individual planktonic cells, biofilms possess notable inherent resistance sanitizers antibiotics. Overcoming this substantial barrier in the medical food industries. Hence, while antibiotics ineffective eradicating P. biofilm, scientists have explored alternate strategies, including utilization natural compounds as novel treatment option. To end, curcumin, carvacrol, thymol, eugenol, cinnamaldehyde, coumarin, catechin, terpinene-4-ol, linalool, pinene, linoleic acid, saponin, geraniol major extensively utilized for management community. Noteworthy, exact interaction bacterium not elucidated yet; however, interference with quorum sensing system inhibition autoinducer production main possible mechanisms. use different drug platforms can overcome some drawbacks compounds, such insolubility water, limited oral bioavailability, fast metabolism, degradation. Additionally, deliver antibiofilm agents simultaneously, which enhances potential compounds. This article explores many facets utilizing inhibit eradicate biofilms. It also examines techniques protocols employed enhance effectiveness these

Language: Английский

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Bioorganic compounds in quorum sensing disruption: strategies, Mechanisms, and future prospects

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108192 - 108192

Published: Jan. 1, 2025

Language: Английский

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Enhancing Commercial Antibiotics with Trans-Cinnamaldehyde in Gram-Positive and Gram-Negative Bacteria: An In Vitro Approach

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 192 - 192

Published: Jan. 10, 2024

One strategy to mitigate the emergence of bacterial resistance involves reducing antibiotic doses by combining them with natural products, such as trans-cinnamaldehyde (CIN). The objective this research was identify in vitro combinations (CIN + commercial (ABX)) that decrease minimum inhibitory concentration (MIC) seven antibiotics against 14 different Gram-positive and Gram-negative pathogenic bacteria, most classified ESKAPE. MIC values were measured for all compounds using broth microdilution method. effect on these microorganisms analyzed through checkboard assay determine type activity (synergy, antagonism, or addition). This analysis complemented a kinetic study synergistic combinations. Fifteen characterized nine tested bacteria. CIN demonstrated effectiveness chloramphenicol, streptomycin, amoxicillin, erythromycin (94-98%) when

Language: Английский

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Critical review on plant-derived quorum sensing signaling inhibitors in pseudomonas aeruginosa

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 151, P. 107649 - 107649

Published: July 15, 2024

Language: Английский

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Design, green synthesis, and quorum sensing quenching potential of novel 2-oxo-pyridines containing a thiophene/furan scaffold and targeting a LasR gene on P. aeruginosa

RSC Advances, Journal Year: 2023, Volume and Issue: 13(39), P. 27363 - 27384

Published: Jan. 1, 2023

The current trend in fighting bacteria is attacking the virulence and quorum-sensing (QS) signals that control bacterial communication factors, especially biofilm formation. This study reports new Schiff bases tetracyclic rings based on a pyridine pharmacophore by two methods: green approach using CAN conventional method. structure of designed derivatives was confirmed different spectroscopies (IR 1H/13C NMR) elemental analysis. exhibited good to moderate inhibition zones against fungal pathogens. In addition, six compounds 2a,b, 3a,b, 6a,b displayed potency tested pathogens with eligible MIC MBC values compared standard antimicrobial agents. Compound 2a 15.6 μg mL-1 Gentamicin (MIC = 250 K. pneumoniae), while compound 6b super-potent activity P. aeruginosa, pneumoniae 62.5 125 mL-1, as well 31.25 mL-1), respectively. Surprisingly, these revealed bactericidal fungicidal remarkable anti-biofilm could significantly reduce formation MRSA, E. coli, C. albicans. Furthermore, most active reduced LasR gene's production between 10-40% at 1/8 MICs untreated aeruginosa. Besides, they demonstrated promising safety profile Vero cells (normal cell lines) IC50 ranging (175.17 ± 3.49 344.27 3.81 mL-1). silico ADMET prediction carried out results be used oral bioavailability low toxicity when administered candidate drug. Finally, molecular docking simulation performed inside predicted key binding interactions responsible for corroborated biological results.

Language: Английский

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Essential Oil Compounds in Combination with Conventional Antibiotics for Dermatology

Molecules, Journal Year: 2024, Volume and Issue: 29(6), P. 1225 - 1225

Published: March 8, 2024

Antimicrobial resistance has emerged as a significant threat to public health, prompting novel combinations comprising of natural sources such essential oil compounds with conventional antibiotics. This study aimed determine the possible interactions between six eight antibiotics/antifungals against pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Acinetobacter baumannii, Cutibacterium acnes, and Candida albicans) commonly implicated in skin infections. The minimum inhibitory concentrations (MICs) for antibiotics were evaluated singularly combination using broth microdilution assay. fractional (FIC) calculated interactive profile combinations. synergistic (FIC ≤ 0.5) further analysed at varying ratios depicted on isobolograms. toxicity was determined brine shrimp lethality Eight identified selected Gram-positive P. aeruginosa pathogens, also demonstrated reduced toxicity. amoxicillin eugenol lowest (LC50 = 1081 µg/mL) highest selectivity index (14.41) when 70:30 ratio. provides insight into vitro antimicrobial that can form basis newer therapies.

Language: Английский

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