New strategies for the treatment of infections caused by biofilm-producing Klebsiella pneumoniae DOI Creative Commons
Т. В. Фадеева, A. V. Nevezhina

Acta Biomedica Scientifica (East Siberian Biomedical Journal), Journal Year: 2024, Volume and Issue: 9(6), P. 63 - 75

Published: Dec. 28, 2024

Treatment of Klebsiella pneumoniae infections is becoming increasingly challenging due to their multiple resistance current antimicrobials. The ability form biofilms a critical virulence feature K. pneumoniae. Biofilms are complex bacterial communities consisting one or more species embedded in an extracellular matrix proteins, carbohydrates, and DNA. Inhibition killing biofilm-producing strains with antibiotics often requires higher concentrations than those required suppress planktonic bacteria. Dosage increases can vary significantly depending on many factors. Therefore, alternative treatments have been sought recently. In this review, the literature was analyzed gain insight into major factors emphasis role enhancing antimicrobial resistance, highlighting importance mechanism for adaptation. search conducted using electronic information resources PubMed, Google Scholar eLibrary. depth limited from 2000 present, share last 5 years 63 %. keywords used were: pneumoniae, biofilm, factors, infection treatment, combination therapy. concepts difference between pathotypes hypervirulent classical, relationship biofilm formation revealed. composition regulation characterized, some influencing structure briefly described. Some new strategies treatment caused by biofilm-forming also presented. Understanding effect antimicrobials paramount clinical practice increased level spread among infectious agents.

Language: Английский

Unveiling the potential of spirulina algal extract as promising antibacterial and antibiofilm agent against carbapenem-resistant Klebsiella pneumoniae: in vitro and in vivo study DOI Creative Commons

M. Selim,

Tarek El-banna,

Fatma Sonbol

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 5, 2025

Abstract Carbapenem-resistant Klebsiella pneumoniae poses a severe risk to global public health, necessitating the immediate development of novel therapeutic strategies. The current study aimed investigate effectiveness green algae Arthrospira maxima (commercially known as Spirulina ) both in vitro and vivo against carbapenem-resistant K. . In this study, thirty isolates were collected, identified, then screened for their susceptibility several antibiotics carbapenemase production genes using PCR. Both bla KPC OXA-48 most predominant detected tested isolates. phytochemical profiling A. algal extract was conducted LC–MS/MS positive mode technique. minimum inhibitory concentrations (MIC) ranged from 500 1000 µg/mL. also resulted decreasing membrane integrity distortion bacterial cells revealed by scanning electron microscope. bioactive compounds that responsible antibacterial action fatty acids, including PUFAs, polysaccharides, glycosides, peptides, flavonoids, phycocyanin, minerals, essential amino vitamins. Moreover, an antibiofilm activity crystal violet assay qRT-PCR. A murine pneumonia model employed assessment extract. showed promising which comparable colistin (standard drug). This manifested improving pulmonary architecture, inflammatory cell infiltration, fibrosis after staining with hematoxylin eosin Masson’s trichrome stain. Using immunohistochemical investigations, percentage immunoreactive significantly decreased monoclonal antibodies tumor necrosis factor-alpha interleukin six. So, may be considered new candidate medications. Graphical

Language: Английский

Citations

2
Hypervirulent and carbapenem-resistant Klebsiella pneumoniae: A global public health threat DOI

Ting-yu Lei,

Bin-bin Liao,

Liang-Rui Yang

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127839 - 127839

Published: Aug. 11, 2024

Language: Английский

Citations

11
Temperature sensing and virulence regulation in pathogenic bacteria DOI Creative Commons
Davide Roncarati, Andrea Vannini, Vincenzo Scarlato

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Pathogenic bacteria can detect a variety of environmental signals, including temperature changes. While sudden and significant variations act as danger signals that trigger protective heat-shock response, minor fluctuations typically signal to the pathogen it has moved from one environment another, such entering specific niche within host during infection. These latter are utilized by pathogens coordinate expression crucial virulence factors. Here, we elucidate critical role in governing factors bacterial pathogens. Moreover, outline molecular mechanisms used fluctuations, focusing on systems employ proteins nucleic acids sensory devices. We also discuss potential implications extent risk climate change poses human pathogenic diseases.

Language: Английский

Citations

7
Design and synthesis of new 1,3,4-thiadiazoles as antimicrobial and antibiofilm agents DOI
Betül Kaya, Ulviye Acar Çevik, Pervin Soyer

et al.

Zeitschrift für Naturforschung C, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Abstract This study assessed the in vitro antibacterial and antibiofilm properties of new 1,3,4-thiadiazole derivatives. 1 H NMR 13 C analyses were employed to confirm structure synthesized compounds, characterization is followed by assessments their efficacy against Bacillus subtilis NRRL B478, Staphylococcus aureus ATCC 29213, Escherichia coli 25922, Pseudomonas aeruginosa 27853, as well for antifungal activity Candida albicans 90028 krusei 6258, using broth microdilution method. Notably, among tested compound 4a exhibited highest antimicrobial activity, with a minimal inhibitory concentration 125 μg/mL P. 27853 significant achieving 94 %, 98 % 100 biofilm inhibition at 250, 500 1,000 μg/mL, respectively. Besides, 4h achieved 81 89 S. 29213 respectively, displayed potent this bacterium. Finally, theoretical ADME compounds - analyzed calculations. has consolidated base development agents advanced our understanding potential mode action biofilm-associated infections.

Language: Английский

Citations

0
Unveiling the potential antibacterial action of acetylcysteine for managing Staphylococcus aureus wound infections: in vitro and in vivo study DOI
Rehab Ahmed, Engy Elekhnawy

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)

Published: April 1, 2025

Language: Английский

Citations

0
Antibiotic conjugates: Using molecular Trojan Horses to overcome drug resistance DOI
Xi Li, Siyuan Dong, Qi Pan

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 186, P. 118007 - 118007

Published: April 21, 2025

Language: Английский

Citations

0
Advancements in nanosensors for detecting pathogens in healthcare environments DOI Creative Commons
Antonia Bruno, Farida Tripodi, Alice Armanni

et al.

Environmental Science Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

ESKAPEE pathogens: where we can find them in hospital environments and how to detect through nanotechnologies devices.

Language: Английский

Citations

2
Evaluation of mrkD, pgaC and wcaJ as biomarkers for rapid identification of K. pneumoniae biofilm infections from endotracheal aspirates and bronchoalveolar lavage DOI Creative Commons
Naveen Kumar Devanga Ragupathi,

Dhiviya Prabaa Muthuirulandi Sethuvel,

Anju Ganesan

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 9, 2024

Abstract Klebsiella pneumoniae has been identified as one of the most important opportunistic pathogens responsible for nosocomial infections. Antibiotic resistance and ability to form biofilms are two main factors involved in persistence Conventional detection methods involve culture isolation identification followed by biofilm assay that takes 48–72 h. Timely biofilm-forming resistant is essential appropriately treat infection with right dose combinations. The present study focuses on evaluating an RT-PCR panel using mrk D, pga C, wca J genes screen K. from ETA/BAL specimens. accurately harboring samples a limit 1 ng/µl total RNA. Representative culture-negative-PCR-positive were subjected metagenomics which reads these confirming specificity RT-PCR. D C act specific whereas acts negative marker . In addition, results correlated well phenotypic assay. This first its kind rapid result this highlights based coupled clinical conditions would be appropriate emerging infections or prevent re-infections settings.

Language: Английский

Citations

2
Fluoroquinolones and Biofilm: A Narrative Review DOI Creative Commons
Nicholas Geremia, F Giovagnorio, Agnese Colpani

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1673 - 1673

Published: Dec. 11, 2024

Background: Biofilm-associated infections frequently span multiple body sites and represent a significant clinical challenge, often requiring multidisciplinary approach involving surgery antimicrobial therapy. These are commonly healthcare-associated related to internal or external medical devices. The formation of biofilms complicates treatment, as they create environments that difficult for most agents penetrate. Fluoroquinolones play critical role in the eradication biofilm-related infections. Numerous studies have investigated synergistic potential combining fluoroquinolones with other chemical augment their efficacy while minimizing toxicity. Comparative research suggests antibiofilm activity is superior beta-lactams glycopeptides. However, remains less effective than minocycline fosfomycin. Noteworthy combinations include fosfomycin aminoglycosides enhanced against Gram-negative organisms rifampin more treatment Gram-positive Despite limitations due intrinsic characteristics this antibiotic, remain fundamental setting thanks bioavailability synergisms drugs. Methods: A comprehensive literature search was conducted using online databases (PubMed/MEDLINE/Google Scholar) books written by experts microbiology infectious diseases identify relevant on biofilm. Results: This review critically assesses managing biofilm-associated various settings also exploring benefits combination therapy these antibiotics. Conclusions: predominantly consists vitro studies, limited vivo investigations. Although real world data scarce, accordance fluoroquinolones’ effectiveness early Also, future perspectives newer options be placed alongside discussed. underscores infections, providing guide physicians regarding best use class antibiotics highlighting existing issues.

Language: Английский

Citations

2
Prevalence and molecular detection of Klebsiella pneumoniae isolated from children with infectious diarrhoea in AL-Ramadi city DOI Creative Commons

Fatima Sabri Al-Omar

IP International Journal of Medical Microbiology and Tropical Diseases, Journal Year: 2024, Volume and Issue: 10(2), P. 108 - 113

Published: June 15, 2024

: Infectious diarrhoea result from a wide range of bacteria, viruses and parasites. This condition is also identified as gastroenteritis, well-known one most common bacterial pathogens causing gastroenteritis. study aims to estimate the incidence identifying both phenotypic genotypic characterization gastroenteritis in children under age five al-Ramadi Maternity Children Teaching Hospital. Stool samples were collected for 106 suffering Cultural microscopical approaches used selection, its characteristic features confirmed using Vitek2 compact system, anti-microbial sensitivity test, biofilm production test. furthermore, DNA extracted, purification Polymerase chain reaction (PCR) accomplished confirmation. In presented research, stool 100 source The bacterium consideration () has percentage 30%. isolate revealed resistance Ceftazidime (80%), nalidixic acid (33%), amikacin (36%), Azithromycin (20%), vancomycin (10%), Imipenem (6%). Likewise, four genes studied via PCR results indicates htrA, iss, Mrka rmpA 15 (50%), 9 (30%), 6 0 (0%) respectively. exposed that 3 (10%) strong, 10 (33%) moderate, 7 (24%) weak, non-producers. research displayed higher rate commonly antibiotics addition, (under consideration) with high showed PCR, addition strong production.

Language: Английский

Citations

0