Toxics,
Journal Year:
2023,
Volume and Issue:
11(12), P. 1001 - 1001
Published: Dec. 8, 2023
The
use
of
bacteria
the
genus
Pseudomonas-destructors
persistent
pollutants
for
biotechnologies
environmental
purification-is
an
interesting
area
research.
aim
this
work
was
to
study
potential
Pseudomonas
mosselii
strain
5(3)
isolated
from
pesticide-contaminated
soil
as
a
degrader
C7-C10
perfluorocarboxylic
acids
(PFCAs)
and
analyze
its
complete
genome.
genome
has
been
fully
sequenced.
It
consists
chromosome
with
length
5,676,241
b.p.
containing
total
5134
genes,
in
particular,
haloalkane
dehalogenase
gene
(dhaA),
haloacetate
H-1
(dehH1),
fluoride
ion
transporter
(crcB)
alkanesulfonate
monooxygenase
(ssuE),
responsible
degradation
fluorinated
compounds.
P.
cultivated
7
days
liquid
medium
various
PFCAs
sole
source
carbon
energy,
completely
disposed
them.
results
LC-MS
analysis
showed
that
transformation
takes
place
due
perfluorohexanoic
acid
release
levels
stoichiometry
(depending
on
PFCA)
fluorine
mineralization
indicators
determined
by
chromatography.
Thus,
demonstrates
genetically
confirmed
high
decomposition
PFCA.
Frontiers in Endocrinology,
Journal Year:
2023,
Volume and Issue:
14
Published: Feb. 20, 2023
As
emerging
organic
contaminants,
per-
and
polyfluoroalkyl
substances
(PFASs)
have
aroused
worldwide
concern
due
to
their
environmental
persistence,
ubiquitous
presence,
bioaccumulation,
potential
toxicity.
It
has
been
demonstrated
that
PFASs
can
accumulate
in
human
body
cause
multiple
adverse
health
outcomes.
Notably,
detected
the
semen
of
human,
posing
a
hazard
male
fecundity.
This
article
reviews
evidence
about
toxic
effects
exposure
on
reproduction,
focusing
sperm
quality.
Epidemiological
studies
showed
PFASs,
such
as
perfluorooctanoic
acid
(PFOA)
perfluorooctane
sulfonic
(PFOS),
were
adversely
associated
with
parameters
humans,
including
count,
morphology
motility.
Experimental
results
also
confirmed
PFAS
led
testicular
epididymal
damage,
therefore
impairing
spermatogenesis
The
mechanisms
reproductive
toxicity
may
be
involved
blood-testosterone
barrier
destruction,
apoptosis,
testosterone
synthesis
disorder,
membrane
lipid
composition
alteration,
oxidative
stress
Ca
2+
influx
sperm.
In
conclusion,
this
review
highlighted
threat
spermatozoa.
Environment International,
Journal Year:
2024,
Volume and Issue:
186, P. 108577 - 108577
Published: March 16, 2024
Male
fertility
has
been
declining
worldwide
especially
in
countries
with
high
levels
of
endocrine
disrupting
chemicals
(EDCs).
Per-
and
polyfluorinated
alkyl
Substances
(PFAS)
have
classified
as
EDCs
linked
to
adverse
male
reproductive
health.
The
mechanisms
these
associations
their
implications
on
offspring
health
remain
unknown.
aims
the
current
study
were
assess
effect
PFAS
mixtures
sperm
methylome
transcriptional
changes
metabolic
tissues
(i.e.,
liver
fat).
C57BL/6
mice
exposed
a
mixture
(PFOS,
PFOA,
PFNA,
PFHxS,
Genx;
20
µg/L
each)
for
18-weeks
or
water
control.
Genome-wide
methylation
was
assessed
F0
epidydimal
using
reduced
representation
bisulfite
sequencing
(RRBS)
Illumina
mouse
array,
while
gene
expression
by
bulk
RNA
8-week-old
derived
from
unexposed
females.
resulted
2,861
83
(Illumina)
DMRs
(q
<
0.05).
Functional
enrichment
revealed
that
PFAS-induced
associated
behavior
developmental
pathways
RRBS,
related
lipid
metabolism
cell
signaling.
Additionally,
40
53
differentially
expressed
genes
(DEGs)
fat
males,
9
31
DEGs
females,
respectively.
alterations
cholesterol
mitotic
cycle
regulation
myeloid
leukocyte
migration
offspring,
female
erythrocyte
development
carbohydrate
catabolism
affected
fat.
Our
results
demonstrate
exposure
legacy
newly
emerging
adult
result
aberrant
altered
sex-specific
manner.
These
data
indicate
preconception
males
can
be
transmitted
affect
phenotype
next
generation.
Although
epidemiological
studies
have
explored
the
association
between
poly-
and
perfluoroalkyl
substances
(PFAS)
concentrations
semen
quality,
existing
findings
are
often
inconsistent.
Our
work
aimed
to
explore
of
PFAS
in
plasma
with
repeated
measures
quality
parameters
healthy
adults.
Plasma
was
collected
at
initial
recruitment
least
once
within
five
predetermined
intervals
during
an
approximately
3-month
period.
Semen
volume,
concentration,
motility,
total
sperm
count
were
measured
each
specimen.
individual
samples
(n
=
1252)
pooled
1019)
from
multiple
collections
(or
a
single
sample
if
no
collection
available).
The
results
reveal
seminal
perfluorooctanoic
acid,
perfluorooctanesulfonic
perfluorohexanesulfonic
6:2
chlorinated
polyfluoroalkyl
ether
sulfonate
significantly
associated
decreased
progressive
motility
while
positively
concentration
count.
By
contrast,
null
associations
observed
quality.
study
provides
evidence
that
exposure
may
affect
male
fertility
should
be
for
precise
assessment
impact
on
reproductive
performances.
Best Practice & Research Clinical Obstetrics & Gynaecology,
Journal Year:
2022,
Volume and Issue:
86, P. 102298 - 102298
Published: Dec. 16, 2022
Semen
quality
has
declined
especially
among
Western
men.
Experimental
and
epidemiological
studies
have
shown
potential
links
between
exposure
to
environmental
toxicants
poor
male
fertility.
Some
exposures
in
utero
can
disrupt
fetal
testicular
function
result
cryptorchidism,
low
semen
quality,
serum
testosterone
levels,
Environmental
childhood
adulthood
also
adversely
affect
germ
cells,
Sertoli
Leydig
or
the
hypothalamic-pituitary-testicular
axis,
resulting
impaired
In
this
review,
we
report
latest
results
from
human
that
investigated
role
of
endocrine
disrupting
chemicals,
heavy
metals,
tobacco
smoking,
alcohol
drinking,
use
marijuana
Current
evidence
suggests
relationship
these
factors
fertility;
however,
some
showed
conflicting
which
need
further
investigation.
Environment International,
Journal Year:
2023,
Volume and Issue:
173, P. 107857 - 107857
Published: Feb. 28, 2023
Perfluoroalkyl
substances
(PFAS)
are
persistent
and
pose
a
risk
to
human
health.
High
throughput
screening
(HTS)
cell-based
bioassays
may
inform
assessment
of
PFAS
provided
that
quantitative
in
vitro
vivo
extrapolation
(QIVIVE)
can
be
developed.
The
QIVIVE
ratio
is
the
nominal
(Cnom)
or
freely
dissolved
concentration
(Cfree)
blood
Cnom
Cfree
bioassays.
Considering
concentrations
plasma
vary
by
orders
magnitude,
we
tested
hypothesis
anionic
bind
proteins
concentration-dependently
therefore
binding
differs
substantially
between
bioassays,
which
has
an
impact
on
QIVIVE.
Solid
phase
microextraction
(SPME)
with
C18-coated
fibers
served
quantify
four
(perfluorobutanoate
(PFBA),
perfluorooctanoate
(PFOA),
perfluorohexane
sulfonate
(PFHxS)
perfluorooctane
(PFOS))
presence
lipid,
medium
components,
cells
over
five
magnitude
concentrations.
C18-SPME
method
was
used
non-linear
proteins,
medium,
partition
constants
cells.
These
parameters
were
predict
cell
concentration-dependent
mass
balance
model
(MBM).
approach
illustrated
reporter
gene
assay
indicating
activation
peroxisome
proliferator-activated
receptor
gamma
(PPARγ-GeneBLAzer).
Blood
levels
collected
from
literature
for
occupational
exposure
general
population.
QIVIVEnom
ratios
higher
than
QIVIVEfree
due
strong
affinity
large
differences
protein
contents
For
health
assessment,
many
assays
need
combined
cover
all
relevant
endpoints.
If
cannot
measured,
they
estimated
MBM
distribution
ratios.
Toxicological Sciences,
Journal Year:
2024,
Volume and Issue:
200(1), P. 57 - 69
Published: April 11, 2024
Abstract
Per-
and
polyfluoroalkyl
substances
(PFAS)
are
a
group
of
synthetic
chemicals
that
resistant
to
biodegradation
environmentally
persistent.
PFAS
found
in
many
consumer
products
major
source
water
soil
contamination.
This
study
investigated
the
effects
an
relevant
mixture
(perfluorooctanoic
acid
[PFOA],
perfluorooctanesulfonic
[PFOS],
perfluorohexanesulfonic
[PFHxS])
on
transcriptome
function
human
granulosa
cells
(hGCs).
Primary
hGCs
were
harvested
from
follicular
aspirates
healthy,
reproductive-age
women
who
undergoing
oocyte
retrieval
for
vitro
fertilization.
Liquid
Chromatography
with
tandem
mass
spectrometry
(LC/MS-MS)
was
performed
identify
compounds
pure
fluid.
Cells
cultured
vehicle
control
or
(2
nM
PFHxS,
7
PFOA,
10
PFOS)
96
h.
Analyses
cell
proliferation/apoptosis,
steroidogenesis,
gene
expression
measured
via
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide
assays/immunofluorescence,
ELISA/western
blotting,
RNA
sequencing/bioinformatics,
respectively.
PFOS,
PFHxS
detected
100%
follicle
fluid
samples.
Increased
proliferation
observed
treated
no
impacts
cellular
apoptosis.
The
also
altered
steroid
hormone
synthesis,
increasing
both
follicle-stimulating
hormone-stimulated
basal
progesterone
secretion
concomitant
upregulation
STAR
protein.
sequencing
revealed
inherent
differences
transcriptomic
profiles
after
exposure.
demonstrates
functional
changes
exposure
mixture,
improving
our
knowledge
about
exposures
female
reproductive
health.
These
findings
suggest
can
disrupt
normal
possible
long-term
consequences
overall
