SIRT6 Overexpression Enhances Diabetic Foot Ulcer Healing via Nrf2 Pathway Activation DOI Creative Commons
Wěi Li,

Mengyang Kang,

Guofeng Zhang

et al.

Inflammation, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Sirtuin-6 (SIRT6) has a pivotal role in wide array of cellular biological functions and is linked to the progression various diseases. Previous findings have identified SIRT6 as protective modulator against numerous diabetic complications. However, whether exerts foot ulcer (DFU) remains unstudied. This work established rat model DFU evaluated possible mediating wound healing DFU. Marked reductions levels were observed samples from patients rats. Increasing expression tissues remarkably decreased area, accelerated epithelialisation, increased collagen deposition improved angiogenesis. Moreover, up-modulation relieved oxidative stress inflammation The increase cultured vascular endothelial cells restrained cell apoptosis, elicited by high glucose (HG). HG-impaired migration capacity angiogenesis was also recovered increasing expression. Mechanism research revealed that overexpression reinforced activation Nrf2 pathway rats HG-exposed cells. Pharmacological suppression reversed effect on HG-triggered dysfunction. this indicate positive related which contributes improvement study highlights previously unaddressed healing, providing novel insights into its functions. hold significant clinical value identifying promising therapeutic target for improving healing.

Language: Английский

NIR-responsive electrospun nanofiber dressing promotes diabetic-infected wound healing with programmed combined temperature-coordinated photothermal therapy DOI Creative Commons

Jinlang Fu,

Ding Wang,

Zinan Tang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 1, 2024

Abstract Background Diabetic wounds present significant challenges, specifically in terms of bacterial infection and delayed healing. Therefore, it is crucial to address local issues promote accelerated wound In this investigation, we utilized electrospinning fabricate microgel/nanofiber membranes encapsulating MXene-encapsulated microgels chitosan/gelatin polymers. Results The film dressing facilitates programmed photothermal therapy (PPT) mild (MPTT) under near-infrared (NIR), showcasing swift extensive antibacterial biofilm-disrupting capabilities. PPT effect achieves prompt sterilization within 5 min at 52 °C disperses mature biofilm 10 min. Concurrently, by adjusting the NIR power induce heating (42 °C), stimulates fibroblast proliferation migration, significantly enhancing vascularization. Moreover, vivo experimentation successfully validates dressing, underscoring its immense potential addressing intricacies diabetic wounds. Conclusions MXene microgel-loaded nanofiber employs temperature-coordinated therapy, effectively amalgamating advantageous features high-temperature low-temperature promotion It exhibits rapid, broad-spectrum capabilities, exceptional biocompatibility, noteworthy effects on promoting cell These results affirm efficacy our highlighting challenge struggling heal due infection. Graphical

Language: Английский

Citations

12
Curcumin Promotes Diabetic Foot Ulcer Wound Healing by Inhibiting miR-152-3p and Activating the FBN1/TGF-β Pathway DOI Creative Commons
Mei Cao,

Zhisheng Duan,

Xianting Wang

et al.

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: 66(5), P. 1266 - 1278

Published: Jan. 11, 2024

Abstract The objective of this study was to investigate the mechanism curcumin in diabetic foot ulcer (DFU) wound healing. A DFU rat model established, and fibroblasts were cultured a high-glucose (HG) environment create cell model. Various techniques, including Western blot, RT‒qPCR, flow cytometry, Transwell, scratch test H&E staining, employed measure levels relevant genes proteins, as well assess proliferation, apoptosis, migration, pathological changes. results showed that miR-152-3p overexpressed patients, while FBN1 underexpressed. Curcumin found inhibit fibroblast promote angiogenesis rats, accelerate healing rats. In addition, overexpression weakened therapeutic effect curcumin, reversed effects mimic. Further investigations into underlying mechanisms revealed expedited rats by restoring FBN1/TGF-β pathway through inhibition miR-152-3p. conclusion, can suppress activity miR-152-3p, which, turn, leads rejuvenation accelerates

Language: Английский

Citations

10
Insights of biopolymeric blended formulations for diabetic wound healing DOI
Ameya Sharma, Divya Dheer, Vivek Puri

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 656, P. 124099 - 124099

Published: April 16, 2024

Language: Английский

Citations

10
Dissolvable microneedle-based wound dressing transdermally and continuously delivers anti-inflammatory and pro-angiogenic exosomes for diabetic wound treatment DOI Creative Commons
Yanpeng Cao, Bei Chen,

Qixing Liu

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 32 - 51

Published: Aug. 27, 2024

Due to overactive inflammation and hindered angiogenesis, self-healing of diabetic wounds (DW) remains challenging in the clinic. Platelet-derived exosomes (PLT-Exos), a novel exosome capable anti-inflammation pro-angiogenesis, show great potential DW treatment. However, previous administration into skin is topical daub or intradermal injection, which cannot intradermally deliver PLT-Exos dermis layer, thus impeding its long-term efficacy pro-angiogenesis. Herein, dissolvable microneedle-based wound dressing (PLT-Exos@ADMMA-MN) was developed for transdermal delivery PLT-Exos. Firstly, photo-crosslinking methacrylated acellular dermal matrix-based hydrogel (ADMMA-GEL), showing physiochemical tailorability, fast-gelling performance, excellent biocompatibility, pro-angiogenic capacities, synthesized as base material our dressing. For endowing with were encapsulated ADMMA-GEL minimum effective concentration determined by in-vitro experiments. Then, results that this exhibits properties Lastly, in-vivo experiments showed could continuously transdermally switch local macrophage M2 phenotype while stimulating neovascularization, proving low-inflammatory microenvironment healing. Collectively, study provides suppressing vascularization

Language: Английский

Citations

9
Research progress and application of chitosan dressings in hemostasis: A review DOI
Wenwen Zhang,

Xinrong Geng,

Song Qin

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 136421 - 136421

Published: Oct. 9, 2024

Language: Английский

Citations

9
Engineered exosomes as a prospective therapy for diabetic foot ulcers DOI Creative Commons

Lifei Guo,

Dan Xiao,

Helin Xing

et al.

Burns & Trauma, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 1, 2024

Abstract Diabetic foot ulcer (DFU), characterized by high recurrence rate, amputations and mortality, poses a significant challenge in diabetes management. The complex pathology involves dysregulated glucose homeostasis leading to systemic local microenvironmental complications, including peripheral neuropathy, micro- macro-angiopathy, recurrent infection, persistent inflammation re-epithelialization. Novel approaches accelerate DFU healing are actively pursued, with focus on utilizing exosomes. Exosomes natural nanovesicles mediating cellular communication containing diverse functional molecular cargos, DNA, mRNA, microRNA (miRNA), lncRNA, proteins, lipids metabolites. While some exosomes show promise modulating function promoting healing, their efficacy is limited low yield, impurities, loading content inadequate targeting. Engineering enhance curative activity represents potentially more efficient approach for DFUs. This could facilitate focused repair regeneration of nerves, blood vessels soft tissue after development. review provides an overview pathogenesis, strategies exosome engineering the targeted therapeutic application engineered addressing critical pathological changes associated

Language: Английский

Citations

8
Advanced multifunctional hydrogels for diabetic foot ulcer healing: Active substances and biological functions DOI Creative Commons

Yuetong Li,

Yuxin Leng, Liu Yang

et al.

Journal of Diabetes, Journal Year: 2024, Volume and Issue: 16(4)

Published: April 1, 2024

Abstract Aim Hydrogels with excellent biocompatibility and biodegradability can be used as the desirable dressings for therapy of diabetic foot ulcer (DFU). This review aimed to summarize biological functions hydrogels, combining pathogenesis DFU. Methods The studies in last 10 years were searched summarized from online database PubMed using a combination keywords such hydrogel diabetes. hydrogels their healing mechanism on DFU elaborated. Results In this review, classified by active substances drugs, cytokines, photosensitizers, biomimetic peptide. Based this, associating DFU, including oxidative stress, chronic inflammation, cell phenotype change, vasculopathy, infection. also pointed out some shortcomings present researches. Conclusions into carrier self‐functioning review. Besides, components existing clarified provide assistance future researches clinical applications. image

Language: Английский

Citations

6
Diabetic Wound Healing: Navigating Physiology, Advancements and Research Frontiers DOI Open Access
Prabhakar Kumar,

Vijender Kumar

Journal of Diabetes Research Review & Reports, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: April 15, 2024

Diabetes, a multifaceted metabolic disorder characterized by inadequate insulin production or resistance, intricately intertwines with the challenging landscape of wound healing, particularly in context diabetic wounds. This review embarks on comprehensive exploration, commencing an overview diabetes physiology, setting stage for understanding its intricate interplay healing complexities. Navigating through physiology wounds, this encapsulates their classification and molecular cellular mechanisms orchestrating process. However, amidst commendable advancements, discernible research gap remains, urging more nuanced comprehension pathophysiology. Examining treatment approaches, article critically assesses various methods efficacy, rooted recent studies. It explores advanced therapies emerging technologies, envisioning possibilities such as 3D printing smart dressing applications. Nonetheless, integrating these technologies into clinical practice awaits highlighting standardizing advancements. Infection control antibiotics are pivotal management. underscores importance, stressing need refined strategies to optimize antibiotic use combat evolving bacterial resistance. Furthermore, advocating patient-centered care education, emphasizes holistic underscoring persistent seamlessly patient education routine management protocols. offers insights modalities, while gaps demanding further exploration refinement.

Language: Английский

Citations

4
Periosteal distraction as a new surgical technique for the treatment of senile diabetic foot: A retrospective case analysis DOI Creative Commons
Rui Yang, Yue Zhou,

Yunxiang Hu

et al.

Medicine, Journal Year: 2025, Volume and Issue: 104(1), P. e41183 - e41183

Published: Jan. 3, 2025

This study investigates the clinical effect and safety of periosteal distraction in treatment senile diabetic foot. The data 45 patients with foot treated Central Hospital Dalian University Technology from January 2020 to May 2024 were retrospectively analyzed. Finally, 42 followed up, 3 lost follow-up, including 29 males 13 females, aged (71.17 ± 6.43), (62-84) years respectively. Wagner grade ulcer surface affected was 2 25 cases, 4 cases; sites toes 18 soles 14 dorsum 8 heels 1 case, ankles case. toe oxygen saturation, ankle-brachial index (ABI), skin temperature visual analogue score (VAS) recorded before operation at day, 7 days, month, months, months after operation. therapeutic observed evaluated combination Michigan neurological sign lower limb computed tomography angiography. wound healing rate, amputation rate recurrence also counted. ABI, temperature, VAS all significantly improved compared those operation, differences statistically significant (P < .05); After a 3-month post-surgery period, 37 have microcirculation formation limb, as well increased thickened extremity arterioles comparison pre-surgery conditions, forming an interwoven network. During follow-up 21 exhibited healed ulcers post-surgery, while by end 38 had ulcers, 5 experienced reduction area, overall 90%. Notably, no instances or during treatment. Periosteal is new surgical method for ulcer, which can obtain satisfactory short-term efficacy worthy promotion.

Language: Английский

Citations

0
Ghrelin Promotes Chronic Diabetic Wound Healing by Regulating Keratinocyte Proliferation and Migration Through the ERK1/2 Pathway DOI
Yukang Zhang, Yuan Chen, Kailin Li

et al.

Peptides, Journal Year: 2025, Volume and Issue: 184, P. 171350 - 171350

Published: Jan. 15, 2025

Language: Английский

Citations

0