Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: June 26, 2023
Abstract
Background
:
Genome-wide
DNA
methylation
(DNAme)
profiling
of
the
placenta
with
Illumina
Infinium
Methylation
bead
arrays
is
often
used
to
explore
connections
between
in
utero
exposures,
placental
pathology,
and
fetal
development.
However,
many
technical
biological
factors
can
lead
signals
DNAme
variation
samples
cohorts,
understanding
accounting
for
these
essential
ensure
meaningful
replicable
data
analysis.
Recently,
“epiphenotyping”
approaches
have
been
developed
whereby
be
impute
information
about
phenotypic
variables
such
as
gestational
age,
sex,
cell
composition,
ancestry.
These
epiphenotypes
offer
avenues
compare
across
understand
how
relate
variability.
relationships
epiphenotyping
other
variables,
their
application
downstream
epigenome
analyses,
not
well
studied.
Results
Using
from
204
placentas
three
we
applied
PlaNET
R
package
estimate
ancestry,
composition
samples.
ancestry
estimates
were
highly
correlated
independent
polymorphic
informative
markers,
epigenetic
on
average,
was
estimated
within
4
days
reported
underscoring
accuracy
tools.
Cell
varied
both
but
reassuringly
robust
processing
time.
Interestingly,
ratio
cytotrophoblast
syncytiotrophoblast
proportion
decreased
increasing
differed
slightly
by
maternal
ethnicity
(lower
white
vs.
non-white)
genetic
higher
probability
European
ancestry).
The
cohort
origin
largest
drivers
this
dataset,
based
associations
first
principal
component.
Conclusions
This
work
confirms
that
cohort,
array
(technical)
batch,
type
proportion,
self-reported
ethnicity,
sex
are
important
consider
any
analyses
data.
Further,
demonstrate
estimating
epiphenotype
itself,
when
possible,
provides
an
check
clinically-obtained
provide
a
approach
different
datasets.
Signal Transduction and Targeted Therapy,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: Feb. 28, 2024
Metabolic
diseases
and
their
complications
impose
health
economic
burdens
worldwide.
Evidence
from
past
experimental
studies
clinical
trials
suggests
our
body
may
have
the
ability
to
remember
metabolic
environment,
such
as
hyperglycemia
or
hyperlipidemia,
thus
leading
chronic
inflammatory
disorders
other
even
after
elimination
of
these
environments.
The
long-term
effects
that
aberrant
metabolism
on
been
summarized
memory
are
found
assume
a
crucial
role
in
states
disease.
Multiple
molecular
mechanisms
collectively
participate
management,
resulting
different
cellular
alterations
well
tissue
organ
dysfunctions,
culminating
disease
progression
affecting
offspring.
elucidation
expansion
concept
provides
more
comprehensive
insight
into
pathogenic
underlying
promises
be
new
target
detection
management.
Here,
we
retrace
history
relevant
research
summarize
its
salient
characteristics.
We
provide
detailed
discussion
by
which
involved
development
at
molecular,
cellular,
levels,
with
emphasis
impact
epigenetic
modulations.
Finally,
present
some
pivotal
findings
arguing
favor
targeting
develop
therapeutic
strategies
for
latest
reflections
consequences
implications
human
diseases.
Investigative Ophthalmology & Visual Science,
Journal Year:
2025,
Volume and Issue:
66(2), P. 16 - 16
Published: Feb. 6, 2025
Purpose:
This
study
aims
to
determine
the
impact
of
preconception
paternal
alcohol
consumption
(PPAC)
on
retinal
function
and
morphology
in
PPAC-offspring.
Fetal
spectrum
disorder
(FASD)–related
ocular
defects
caused
by
maternal
exposure
has
been
well
investigated,
but
influence
PPAC
offspring
eyes
remains
unknown.
Methods:
Adult
C57BL/6J
male
mice
were
exposed
either
10%
ethanol
or
water
(control)
for
six
weeks
bred
naïve
females.
Dark-adapted
light
responses
at
two,
four,
months
old
assessed
using
electroretinography
(ERG)
born
control
males.
The
thicknesses
whole
retinas
different
layers
PPAC-offspring
analyzed
two
old.
Results:
Some
had
only
one
developed
eye.
ERG
a-
b-wave
amplitudes
reduced
compared
controls,
with
a
more
pronounced
effect
significant
effects
inner
function.
At
old,
there
was
thinning
nuclear
plexiform
thickness
similar
between
both
treatment
groups.
Conclusions:
provides
pioneering
evidence
that
contributes
FASD-related
including
negative
impacts
young
adult
offspring.
Thus
adverse
prior
conception
their
(from
childhood
early
adulthood)
should
be
considered
as
seriously
contribution
FASD.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(11), P. 1445 - 1445
Published: Nov. 13, 2024
Peroxisome
proliferator-activated
receptors
(PPARs)
belong
to
a
family
of
nuclear
receptors.
To
date,
three
types
PPARs,
namely
PPARα,
PPARδ,
and
PPARγ,
have
been
identified,
demonstrating
co-expression
across
numerous
tissues.
PPARγ
is
primarily
distributed
in
adipose
tissue,
the
colon,
immune
system,
retina,
while
PPARα
predominantly
expressed
metabolic
tissues
such
as
brown
liver,
kidneys.
Both
play
crucial
roles
various
cellular
processes.
Recent
data
suggest
that
PPAR
family,
among
other
mechanisms,
might
also
be
regulated
by
epigenetic
mechanisms.
Our
recent
studies,
alongside
others,
highlighted
pivotal
DNA
methylation
histone
modifications
regulation
implicating
them
deterioration
disorders
via
This
still
not
fully
understood
mechanism
has
summarized
described
present
paper.
The
review
summarizes
available
on
elucidating
link
between
development
dysregulation
resulting
from
these
Nutrients,
Journal Year:
2023,
Volume and Issue:
15(19), P. 4094 - 4094
Published: Sept. 22, 2023
This
Special
Issue
of
Nutrients,
"Influence
Maternal
Diet
and
Environmental
Factors
on
Fetal
Development",
requests
articles
the
roles
maternal
diet
environmental
factors
such
as
microbiota,
plastics,
endocrine
disruptive
chemicals
impact
fetal
development
[...].
Epigenetics & Chromatin,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: Oct. 4, 2023
Genome-wide
DNA
methylation
(DNAme)
profiling
of
the
placenta
with
Illumina
Infinium
Methylation
bead
arrays
is
often
used
to
explore
connections
between
in
utero
exposures,
placental
pathology,
and
fetal
development.
However,
many
technical
biological
factors
can
lead
signals
DNAme
variation
samples
cohorts,
understanding
accounting
for
these
essential
ensure
meaningful
replicable
data
analysis.
Recently,
"epiphenotyping"
approaches
have
been
developed
whereby
be
impute
information
about
phenotypic
variables
such
as
gestational
age,
sex,
cell
composition,
ancestry.
These
epiphenotypes
offer
avenues
compare
across
understand
how
relate
variability.
relationships
epiphenotyping
other
variables,
their
application
downstream
epigenome
analyses,
not
well
studied.
Using
from
204
placentas
three
we
applied
PlaNET
R
package
estimate
ancestry,
composition
samples.
ancestry
estimates
were
highly
correlated
independent
polymorphic
ancestry-informative
markers,
epigenetic
on
average,
was
estimated
within
4
days
reported
underscoring
accuracy
tools.
Cell
varied
both
over
very
long
processing
times.
Interestingly,
ratio
cytotrophoblast
syncytiotrophoblast
proportion
decreased
increasing
differed
slightly
by
maternal
ethnicity
(lower
white
vs.
non-white)
genetic
higher
probability
European
ancestry).
The
cohort
origin
largest
drivers
this
dataset,
based
associations
first
principal
component.
This
work
confirms
that
cohort,
array
(technical)
batch,
type
proportion,
self-reported
ethnicity,
sex
are
important
consider
any
analyses
data.
We
further
demonstrate
specific
utility
tools
use
data,
show
(i)
provide
an
check
clinically
obtained
(ii)
a
robust
approach
different
datasets.
Finally,
present
general
framework
analysis
integrating
epiphenotype
discussed
here.
