Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters DOI Creative Commons
Syed Zawar Shah, Thomas N. Perry, Andrea Graziadei

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract RNA polymerase III (Pol III) is specialized in the transcription of short, essential RNAs, including U6 small nuclear RNAs (snRNAs). At snRNA genes, Pol recruited by Activating Protein Complex (SNAPc) forming, together with a Brf2-containing TFIIIB complex, transcriptionally competent pre-initiation complex (PIC). Additionally, SNAPc responsible for recruitment II at remaining snRNAs genes (U1, 2, 4 and 5), representing unique example multi subunit factor shared among different Polymerases. The mechanism cross-polymerase engagement role SNAPC2 SNAPC5 subunits remain poorly defined. Here, we present cryo-EM structures full-length SNAPc-containing PIC assembled on promoter open melting states 3.2-4.2Å resolution. Comparative structural analysis revealed unexpected differences yeast molecular basis selective structurally distinct within PICs. Harnessing crosslinking mass spectrometry, also localize proximity to bound DNA, expanding upon existing descriptions structure.

Language: Английский

Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation DOI Open Access
Liliana Villao-Uzho, Tatiana Chávez-Navarrete,

Ricardo Pacheco-Coello

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(6), P. 1226 - 1226

Published: June 6, 2023

One of the strategies to overcome diseases or abiotic stress in crops is use improved varieties. Genetic improvement could be accomplished through different methods, including conventional breeding, induced mutation, genetic transformation, gene editing. The function and regulated expression promoters are necessary for transgenic improve specific traits. variety promoter sequences has increased generation genetically modified because they lead responsible trait a manner. Therefore, characterization activity biotechnological crops. That why several analyses have focused on identifying isolating using techniques such as reverse transcriptase-polymerase chain reaction (RT-PCR), libraries, cloning, sequencing. Promoter analysis involves plant transformation method, potent tool determining genes plants, contributing understanding regulation development. Furthermore, study that play fundamental role highly relevant. development organisms made it possible understand benefits directing temporal, spatial, even controlled manner, confirming great diversity discovered developed. crucial processes ensure correct gene. This review highlights various types their functionality

Language: Английский

Citations

34
Recent Developments in CRISPR/Cas9 Genome-Editing Technology Related to Plant Disease Resistance and Abiotic Stress Tolerance DOI Creative Commons
İbrahim Erdoğan,

Birsen Cevher-Keskin,

Özlem Bilir

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 1037 - 1037

Published: July 22, 2023

The revolutionary CRISPR/Cas9 genome-editing technology has emerged as a powerful tool for plant improvement, offering unprecedented precision and efficiency in making targeted gene modifications. This practical approach to genome editing offers tremendous opportunities crop surpassing the capabilities of conventional breeding techniques. article provides an overview recent advancements challenges associated with application improvement. potential terms developing crops enhanced resistance biotic abiotic stresses is highlighted, examples genes edited confer disease resistance, drought tolerance, salt cold tolerance. Here, we also discuss importance off-target effects efforts made mitigate them, including use shorter single-guide RNAs dual Cas9 nickases. Furthermore, alternative delivery methods, such protein- RNA-based approaches, are explored, they could potentially avoid integration foreign DNA into genome, thus alleviating concerns related genetically modified organisms (GMOs). We emphasize significance accelerating processes, reducing time costs, enabling introduction desired traits at nucleotide level. As field continues evolve, it anticipated that will remain prominent adaptation challenging environmental conditions.

Language: Английский

Citations

32
Plant Promoters and Terminators for High-Precision Bioengineering DOI Creative Commons
Emily Brooks,

Estefania Elorriaga,

Yang Liu

et al.

BioDesign Research, Journal Year: 2023, Volume and Issue: 5, P. 0013 - 0013

Published: Jan. 1, 2023

High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional posttranscriptional levels. Gene transcription is tightly regulated by promoters terminators. Promoters determine the timing, tissues cells, levels of genes. Terminators mediate termination genes affect mRNA posttranscriptionally, e.g., 3'-end processing, stability, translation efficiency, nuclear to cytoplasmic export mRNAs. The promoter terminator combination affects expression. In present article, we review function features plant core promoters, proximal distal terminators, their effects on benchmarking strategies for regulating

Language: Английский

Citations

29
Progress and challenges in CRISPR/Cas applications in microalgae DOI
Quynh-Giao Tran, Trang Thi Le,

Dong‐Yun Choi

et al.

The Journal of Microbiology, Journal Year: 2025, Volume and Issue: 63(3), P. e2501028 - e2501028

Published: March 28, 2025

Language: Английский

Citations

1
Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding DOI Creative Commons
Junming Zhou,

Xinchao Luan,

Yixuan Liu

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(7), P. 1478 - 1478

Published: March 28, 2023

Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has become increasingly mature is being widely used for crop improvement. The application of CRISPR/Cas9 enables the generation transgene-free genome-edited plants in a short period advantages simplicity, high efficiency, specificity, low production costs, which greatly facilitate study gene functions. In plant molecular breeding, efficiency system proven to be key step influencing effectiveness with improvements recently becoming focus reported scientific research. This review details strategies methods improving editing including Cas9 variant enzyme engineering, effect multiple promoter driven Cas9, gRNA efficient optimization expression strategies. It also briefly introduces CRISPR/Cas12a BE PE precision editing. These are beneficial further development systems field breeding.

Language: Английский

Citations

19
Gene editing tool kit in millets: present status and future directions DOI

Vidhi Sapara,

Mitesh Khisti,

Kalenahalli Yogendra

et al.

The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 157 - 179

Published: April 1, 2024

Language: Английский

Citations

4
Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters DOI Creative Commons
Syed Zawar Shah, Thomas N. Perry, Andrea Graziadei

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

RNA polymerase III (Pol III) transcribes short, essential RNAs, including the U6 small nuclear (snRNA). At snRNA genes, Pol is recruited by Activating Protein Complex (SNAPc) and a Brf2-containing TFIIIB complex, forming pre-initiation complex (PIC). Uniquely, SNAPc also recruits II at remaining splicesosomal genes (U1, 2, 4 5). The mechanism of cross-polymerase engagement role SNAPC2 SNAPC5 subunits remain poorly defined. Here, we present cryo-EM structures full-length SNAPc-containing PIC assembled on promoter in open melting states 3.2–4.2 Å resolution. structural comparison revealed differences with Saccharomyces cerevisiae basis selective within PICs. Additionally, crosslinking mass spectrometry localizes near DNA, expanding upon existing descriptions structure. mediates transcription III. authors have used to resolve structure SNAPc-bound revealing its dual interaction mechanism.

Language: Английский

Citations

0
Eliminating Tissue Culture from Plant Gene Editing in the Near Future: A Wish or Reality? DOI Creative Commons

Nadali Babaeianjelodar,

Jayati Trivedi, Claudia Uhde‐Stone

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100433 - 100433

Published: Jan. 1, 2025

Language: Английский

Citations

0
Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption DOI Creative Commons
K Senthil,

Maniraj Rathinam,

Manisha Parashar

et al.

Journal of Genetic Engineering and Biotechnology, Journal Year: 2025, Volume and Issue: 23(1), P. 100465 - 100465

Published: Feb. 6, 2025

Language: Английский

Citations

0
Overexpression of Ghcad6 in Upland Cotton Enhances Fiber Quality and Increases Lignin Content in Fibers DOI

Zumuremu Tuerxun,

Chenyu Li, Yuanxin Li

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0