Gut microbiota and atopic dermatitis: a two-sample Mendelian randomization study

Frontiers in Medicine, Journal Year: 2023, Volume and Issue: 10

Published: June 22, 2023

Accumulating evidence suggests that alterations in gut microbiota composition and diversity are associated with Atopic dermatitis (AD). But until now, the causal association between them has been unclear.We employed a two-sample Mendelian Randomization (MR) study to estimate potential causality of on AD risk. The summary statistics related were obtained from large-scale genome-wide genotype 16S fecal microbiome dataset 18,340 individuals (24 cohorts) analyzed by MiBioGen Consortium, comprising 211 microbiota. data also derived strictly defined collected FinnGen biobank analysis, which included 218,467 European ancestors (5,321 patients 213,146 controls). inverse variance weighted method (IVW), median (WME), MR-Egger used determine changes pathogenic bacterial taxa, followed sensitivity analysis including horizontal pleiotropy Cochran's Q test, leave-one-out assess reliability results. In addition, MR Steiger's test was suppositional relationship exposure outcome.A total 2,289 SNPs (p < 1 × 10-5) included, 5 taxa 17 characteristics (1 phylum, 3 classes, order, 4 families, 8 genera), after excluding IVs linkage disequilibrium (LD). Combining results IVW models, there 6 biological (2 genera) intestinal flora positively risk 7 2 family, negatively associated. showed Tenericutes, Mollicutes, Clostridia, Bifidobacteriaceae, Bifidobacteriales, Bifidobacterium, Christensenellaceae R group correlated AD, while Clostridiaceae 1, Bacteroidaceae, Bacteroides, Anaerotruncus, unknown genus, Lachnospiraceae UCG001 opposite trend. And robust. above but not vice versa.The present genetically abundance risk, thus only providing support for microecological therapy laying groundwork further exploration mechanisms contributes pathogenesis AD.

Language: Английский

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Gut microbiota and sepsis: bidirectional Mendelian study and mediation analysis

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 17, 2023

Background There is a growing body of evidence that suggests connection between the composition gut microbiota and sepsis. However, more research needed to better understand causal relationship two. To gain deeper insight into association microbiota, C-reactive protein (CRP), sepsis, we conducted several Mendelian randomization (MR) analyses. Methods In this study, publicly available genome-wide study (GWAS) summary statistics were examined determine correlation including various sepsis subgroups (such as under 75, 28-day death, Critical Care Units (ICU), death in ICU). Initially, two-sample reverse analyses identify causality Subsequently, multivariable two-step MR revealed was mediated by CRP. The robustness findings confirmed through sensitivity Findings our positive correlations 24 taxa different outcomes, while 30 demonstrated negative with outcomes. Following correction for multiple testing, found Phylum Lentisphaerae (OR: 0.932, p = 2.64E-03), class Lentisphaeria, order Victivallales 0.927, 1.42E-03) displayed risk. contrast, Tenericutes Mollicutes 1.274, 2.89E-03) positively related risk within 28 days. It notable 1.108, 1.72E-03) also indicated individuals 75. From analysis, it shown (CRP) 32.16% pathway from Additionally, CRP mediate 31.53% effect genus Gordonibacter on Despite these findings, analysis did not indicate any influence levels. Conclusion showcased CRP, which sheds new light potential role mediator facilitating impact

Language: Английский

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Clarifying the effect of gut microbiota on allergic conjunctivitis risk is instrumental for predictive, preventive, and personalized medicine: a Mendelian randomization analysis

The EPMA Journal, Journal Year: 2023, Volume and Issue: 14(2), P. 235 - 248

Published: May 22, 2023

Language: Английский

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Causal association of leisure sedentary behavior and cervical spondylosis, sciatica, intervertebral disk disorders, and low back pain: a Mendelian randomization study

Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 23, 2024

Background Some studies suggest sedentary behavior is a risk factor for musculoskeletal disorders. This study aimed to investigate the potential causal association between leisure (LSB) (including television (TV) viewing, computer use, and driving) incidence of sciatica, intervertebral disk degeneration (IVDD), low back pain (LBP), cervical spondylosis (CS). Methods We obtained data LSB, CS, IVDD, LBP, sciatica proposed mediators from gene-wide (GWAS). The effects were examined by Inverse Variance Weighted (IVW) test, MR-Egger, weighted median, mode simple mode. And sensitivity analysis was performed using MR-Pleiotropy Residual Sum Outlier (MR-PRESSO) MR-Egger intercept test. Multivariable MR (MVMR) conducted independent other LSB; while two-step used explore including Body mass index (BMI), smoking initiation, type 2 diabetes mellitus (T2DM), major depressive disorder (MDD), schizophrenia, bipolar LSB these diseases based on previous studies. Results Genetically associated TV viewing positively with CS (OR = 1.61, 95%CI 1.25 2.07, p 0.002), IVDD 2.10, 1.77 2.48, 3.79 × 10 −18 ), LBP 1.84, 1.53 2.21, 1.04 −10 ) 1.82, 95% CI 1.45 2.27, 1.42 −7 ). While use reduced 0.66, 0.55 0.79, 8.06 −6 0.49, 0.40 0.59, 2.68 −13 0.58, 0.46 0.75, 1.98 −5 Sensitivity validated robustness outcomes. MVMR showed that effect 1.59, 1.13 2.25, 0.008), 2.15, 1.50 3.08, 3.38 1.03 2.52, 0.037) LSB. Furthermore, indicated BMI, T2DM may mediate diseases. Conclusion provides empirical evidence supporting positive which potentially mediated initiation T2DM.

Language: Английский

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Genetic evidence supporting the causal role of gut microbiota in chronic kidney disease and chronic systemic inflammation in CKD: a bilateral two-sample Mendelian randomization study

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 2, 2023

Background The association of gut microbiota (GM) and chronic kidney disease (CKD), the relevancy GM systemic inflammation in CKD, were revealed on basis researches gut–kidney axis previous studies. However, their causal relationships are still unclear. Objective To uncover between as well all known from eligible statistics we performed two-sample Mendelian randomization (MR) analysis. Materials methods We acquired latest most comprehensive summary genome-wide study (GWAS) published materials GWAS involving GM, estimated glomerular filtration rate (eGFR), c-reactive protein (CRP) urine albumin creatine ratio (UACR). Subsequently, MR analysis using inverse-variance weighted (IVW) method was used to determine causality exposure outcome. Based it, additional sensitivity verified significant results, possibility reverse also assessed by during this study. Results At locus-wide significance threshold, IVW suggested that protective factors for CKD included family Lachnospiraceae ( P =0.049), genus Eubacterium eligens group =0.002), Intestinimonas =0.009), Streptococcu =0.003) order Desulfovibrionales =0.001). Simultaneously, results showed LachnospiraceaeUCG010 =0.029) a risk factor CKD. Higher abundance Desulfovibrio =0.048) correlated with higher eGFR; Parasutterella =0.018) UACR; class Negativicutes =0.003), Eisenbergiella =0.021), Selenomonadales CRP levels; Mollicutes (0.024), Prevotellaceae =0.030), phylum Tenericutes =0.024) lower levels CRP. No pleiotropy or heterogeneity found analysis, no Conclusion This highlighted associations within gut-kidney axis, revealed. Meanwhile, expanded specific through searches. With further studies microbiota, they may have potential be new biomarkers targeted prevention

Language: Английский

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