Genes and Cells, Journal Year: 2023, Volume and Issue: unknown
Published: May 9, 2023
и биологическая роль И.А
Language: Русский
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14
Published: Jan. 8, 2024
Neutrophils are the most abundant innate immune cells. Multiple mechanisms allow them to engage a wide range of metabolic pathways for biosynthesis and bioenergetics mediating biological processes such as development in bone marrow antimicrobial activity ROS production NET formation, inflammation tissue repair. We first discuss recent work on neutrophil functions regulate granulopoiesis, migration trafficking well effector functions. then syndromes with impaired that influenced by genetic environmental factors nutrient availability usage. Here, we particularly focus role specific macronutrients, glucose, fatty acids, protein, micronutrients vitamin B3, regulating biology how this regulation impacts host health. A special section review primarily discusses ways deficiencies could impact increase infection susceptibility. emphasize biochemical approaches explore metabolism relation Lastly, opportunities challenges neutrophil-centered therapeutic immune-driven diseases highlight unanswered questions guide future discoveries.
Language: Английский
Citations
23
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2929 - 2929
Published: March 2, 2024
Over the past decade, research has prominently established neutrophils as key contributors to intricate landscape of tumor immune biology. As polymorphonuclear granulocytes within innate system, play a pivotal and abundant role, constituting approximately ∼70% all peripheral leukocytes in humans ∼10–20% mice. This substantial presence positions them frontline defense against potential threats. Equipped with diverse array mechanisms, including reactive oxygen species (ROS) generation, degranulation, phagocytosis, formation neutrophil extracellular traps (NETs), undeniably serve indispensable components system. While these functions enable interact adaptive cells such T, B, NK cells, influencing their functions, they also engage dynamic interactions rapidly dividing cells. Consequently, are emerging crucial regulators both pro- anti-tumor immunity. comprehensive review delves into recent illuminate multifaceted roles neutrophils. It explores microenvironment, shedding light on heterogeneity impact recruitment, progression, modulation. Additionally, underscores anti-tumoral capabilities. Finally, it provides valuable insights clinical therapies targeting neutrophils, presenting promising approach leveraging immunity for enhanced cancer treatment.
Language: Английский
Citations
18
Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1307 - 1307
Published: Oct. 16, 2024
Neutrophil extracellular traps (NETs) are intricate, DNA-based, web-like structures adorned with cytotoxic proteins. They play a crucial role in antimicrobial defense but also implicated autoimmune diseases and tissue injury. The process of NET formation, known as NETosis, is regulated cell death mechanism that involves the release these unique to neutrophils. NETosis heavily dependent on production reactive oxygen species (ROS), which can be generated either through NADPH oxidase (NOX) or mitochondrial pathways, leading NOX-dependent NOX-independent respectively. Recent research has revealed an intricate interplay between ROS production, DNA repair, formation different contexts. UV radiation trigger combined apoptosis, apoNETosis, driven by repair. Similarly, calcium ionophore-induced both repair key components, only partial role. In case bacterial infections, early stages pivotal. Interestingly, serum-free conditions, spontaneous occurs NOX-derived ROS, early-stage inhibition halting process, while late-stage increases it. balance processes appears critical factor regulating pathways being activated depending nature stimulus. These findings not deepen our understanding mechanisms behind suggest potential therapeutic targets for conditions where NETs contribute disease pathology.
Language: Английский
Citations
11
Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: 21(9), P. 4217 - 4237
Published: July 26, 2024
Cancer is a significant health concern, increasingly showing insensitivity to traditional treatments, highlighting the urgent need for safer and more practical treatment options. Ribonucleic acid (RNA) gene therapy drugs have demonstrated promising potential in preclinical clinical trials antitumor by regulating tumor-related expression. However, RNA's poor membrane permeability stability restrict its effectiveness entering being utilized cells. An appropriate delivery system crucial achieving targeted tumor effects. The microenvironment (TME), characterized acidity, hypoxia, enzyme overexpression, elevated glutathione (GSH) concentration, excessive reactive oxygen species (ROS), essential survival. Furthermore, these distinctive features can also be harnessed develop intelligent drug systems. Various nanocarriers that respond TME been designed RNA delivery, advantages of targeting low toxicity. This Review discusses abnormal changes components TME, therapeutic RNAs' roles, underlying mechanisms, latest developments utilizing vectors microenvironments treating tumors. We hope it provides insight into creating optimizing improve their effectiveness.
Language: Английский
Citations
9
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: May 15, 2023
The plant immune response to DNA is highly self/nonself-specific. Self-DNA triggered stronger responses by early signals such as H2O2 formation than nonself-DNA from closely related species. Plants lack known receptors. Therefore, we aimed investigate whether a differential sensing of self-versus nonself fragments damage- versus pathogen-associated molecular patterns (DAMPs/PAMPs) or an activation the DNA-damage (DDR) represents more promising framework understand this phenomenon.We treated Arabidopsis thaliana Col-0 plants with sonicated self-DNA other individuals same ecotype, another A. broccoli. We observed self/nonself-DNA-specific induction and jasmonic acid (JA, hormone controlling wound chewing herbivores) salicylic (SA, systemic acquired resistance, SAR, biotrophic pathogens). Mutant lines lacking Ataxia Telangiectasia Mutated (ATM) ATM AND RAD3-RELATED (ATR) - two DDR master kinases retained JA in treatments but completely failed induce SA. Moreover, situ-damaged that had been bleomycin SA infected virulent bacteria Pseudomonas syringae pv. tomato DC3000 glycinea carrying effector avrRpt2, not H2O2-treated challenged non-virulent P. avrRpt2.We conclude both ATR are required for complete extracellular whereas as-yet unknown mechanism allows self/nonself-differential JA-dependent response.
Language: Английский
Citations
10
Pharmaceuticals, Journal Year: 2022, Volume and Issue: 16(1), P. 26 - 26
Published: Dec. 25, 2022
Nanoparticle-based drug delivery systems, which can overcome the challenges associated with poor aqueous solubility and other harmful side effects of drugs, display potent applications in cataract treatment. Herein, we designed a nanosystem gold nanoparticles containing resveratrol (RGNPs) as an anti-aging agent to delay cataracts. The spherical RGNPs had superior ability inhibit hydrogen peroxide-mediated oxidative stress damage, including reactive oxygen species (ROS) production, malondialdehyde (MDA) generation, glutathione (GSH) consumption lens epithelial cells. Additionally, present data showed that could cellular senescence induced by decreasing protein levels p16 p21, reducing ratio BAX/BCL-2 senescence-associated secretory phenotype (SASP) vitro. Moreover, also clearly relieve sodium selenite-induced opacity rat model. Our indicated cell was reduced cataracts were delayed upon treatment through activating Sirt1/Nrf2 signaling pathway. findings suggested serve ingredient, highlighting their potential development.
Language: Английский
Citations
15
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: June 7, 2023
Reactive oxygen species (ROS) is essential for neutrophil extracellular trap formation (NETosis), and generated either by NADPH oxidases (e.g., during infections) or mitochondria sterile injury) in neutrophils. We recently showed that ultraviolet (UV) radiation, a injury-inducing agent, dose-dependently induced mitochondrial ROS generation, increasing levels of shifted the death from apoptosis to NETosis. Nevertheless, how executes UV-induced NETosis unknown. In this study, we first confirmed UV doses used our experiments ROS, inhibition suppressed (Mitosox, SYTOX, immunocytochemistry, imaging). Next, irradiation extensively oxidized DNA, confocal imaging 8-oxyguanine (8-oxoG) NETs. Immunofluorescence microscopy further DNA repair protein, proliferating cell nuclear antigen, was widely distributed throughout indicating machinery active genome Inhibition specific steps base excision (BER) pathway leading up nick formation, but not later steps, summary, study shows (i) high produced following induces extensive oxidative damage, (ii) early BER nicking results chromatin decondensation Collectively, these findings reveal NOX-independent NETosis, also novel biological mechanism irradiation- -mitochondrial ROS-mediated
Language: Английский
Citations
8
Tree Physiology, Journal Year: 2024, Volume and Issue: 44(7)
Published: June 21, 2024
Epigenetic changes serve as a cellular memory for cumulative cold recognition in both herbaceous and tree species, including bud dormancy. However, most studies have discussed predicted chromatin structure with respect to histone marks. In the present study, we investigated structural dynamics of bona fide determine how plants recognize prolonged chilling during initial stage The vegetative axillary buds 'Fuji' apple, which shows typical low temperature-dependent, but not photoperiod, dormancy induction, were used transcriptional change analyses. results integrated using deep-learning model interpreted statistical models, Bayesian estimation. Although our was constructed small dataset two time points, remodelling due random excluded. involvement nucleosome pivotal contribution cold-driven circadian rhythm-dependent pathways regulated by mobility cis-regulatory elements predicted. These findings may help develop potential genetic targets breeding species less overcome effects short winters global warming. Our artificial intelligence concept can improve epigenetic analysis dataset, especially non-model immature genome databases.
Language: Английский
Citations
1
Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: July 17, 2024
Increases in the virulence and survival of some pathogens presence subinhibitory concentrations antibiotics have been reported. However, research on effects antimicrobial substances derived from traditional Chinese medicine is still insufficient. Glabridin a well-known active isoflavone found licorice roots that possesses wide range biological activities. Therefore, this study, Listeria monocytogenes ( L. ) exposed to glabridin was used as object. The minimum inhibitory concentration (MIC) determined for . We investigated impacts morphology, motility, biofilm formation, adherence, results indicated MIC 31.25 μg/mL. At 1/8, 1/4, or 1/2 MIC, did not affect growth, flagellar production, formation inhibited bacterial swimming swarming motility decreased hemolytic activity reduced culture supernatants. also showed had no toxic effect RAW264.7 cells but intracellular growth cells. Furthermore, triggered ROS production induce MET macrophages. In addition, enhance capacity trigger METs extracellular killing macrophages by METs. Thus, we conclude reduce do exhibit activity. could be an interesting food additive bacteriostatic agent with anti-
Language: Английский
Citations
1
Antioxidants and Redox Signaling, Journal Year: 2023, Volume and Issue: 41(4-6), P. 396 - 427
Published: Sept. 19, 2023
Significance: Extracellular traps (ETs) represent structured frameworks that comprised DNA embellished with histones and granular proteins extruded by immune cells in response to various stimuli. Immune contribute adverse effects of chronic inflammation via ET generation, promoting the release nuclear chromatin, reactive oxygen species (ROS), bioactive into extracellular matrix. Recent Advances: The occurrence formation has been documented across diverse cell types. excessive production ROS during activation these potential initiate substantial damage, culminating chromosome decondensation. inflammatory microenvironment fosters impacting tissue remodeling. studies reveal involvement sustaining persistent inflammation, angiogenesis, initiating thrombotic processes. Critical Issues: This review elucidates participation disease etiology, detailing ROS-dependent ROS-independent mechanisms their contextual manifestations. It discusses cell-derived ETs milieu responses therapies. Furthermore, emphasizes significance as biomarkers envisions prophylactic strategies against ET-associated inflammation. Future Directions: Subsequent investigations are warranted uncover intricate governing resolution through normal physiological Moreover, a comprehensive understanding aberrant pathways driving is imperative. Prospective research endeavors should focus on executing expansive clinical discern both diagnostic prognostic facets diseases, thereby shedding light prospective utility biomarkers.
Language: Английский
Citations
2