Caloric restriction: A potential approach for mitigating neuronal damage: Lesson from cellular model of Alzheimer disease DOI Creative Commons
Apoorv Sharma,

Monika Bhardwaj,

Vijay Kumar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Alzheimer’s disease is a neurodegenerative disorder and characterized by amyloid beta accumulation, synaptic dysfunction, oxidative stress, lacks effective therapies. Caloric restriction mimetics such as fisetin chlorogenic acid, natural polyphenols with antioxidant autophagy-inducing properties, show promise in mitigating age-related diseases. This study investigates their neuroprotective effects against induced toxicity differentiated human neuroblastoma SHSY5Y cells. Amyloid exposure disrupted redox homeostasis, impaired autophagy, mitochondrial exacerbating neuronal degeneration. Fisetin acid treatments reversed these deleterious restoring balance, suppressing reactive oxygen species upregulating critical enzymes like SOD1, GSR, catalase. These compounds also attenuated mitophagy via reduced PINK1 expression restored fusion Mfn2. Autophagy-related pathways were significantly modulated, evidenced increased AMPK decreased mTOR mRNA levels, alongside elevated of ATG101, ATG13, ULK1, P62 ATG5 levels. Docking studies revealed binding CGA within the pockets FKBP12 supporting interaction. Furthermore, improved integrity PSD95 synaptophysin reducing acetylcholinesterase expression. findings highlight potential ameliorating through autophagy activation, preservation, function enhancement. While this demonstrates transcriptional impact affinities caloric further translational biophysical analyses are required to elucidate mechanisms confirm therapeutic viability. research underscores agents, offering promising avenues for combating related neuropathies. Figure

Language: Английский

The Role of Long Non-Coding RNA in the Pathogenesis of Psoriasis DOI Creative Commons
Kajetan Kiełbowski,

Anna Jędrasiak,

Estera Bakinowska

et al.

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(1), P. 7 - 7

Published: Jan. 17, 2025

Psoriasis is a chronic immune-mediated disease with complex pathogenesis. The altered proliferation and differentiation of keratinocytes, together the activity dendritic cells T cells, are crucial drivers psoriasis progression. Long non-coding RNAs (lncRNAs) composed over 200 nucleotides exert large variety functions, including regulation gene expression. Under pathological conditions, expression lncRNAs frequently dysregulated. Recent studies demonstrated that significantly affect major cellular processes, their aberrant likely involved in pathogenesis various disorders. In this review, we will discuss role pathophysiology psoriasis. We summarize recent investigated relationships between keratinocyte pro-inflammatory responses.

Language: Английский

Citations

1
Exploring the role of autophagy in psoriasis pathogenesis: Insights into sustained inflammation and dysfunctional keratinocyte differentiation DOI
Xinxin Wu, Jiankun Song, Ying Zhang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 135, P. 112244 - 112244

Published: May 21, 2024

Language: Английский

Citations

7
MATN3 delivered by exosome from synovial mesenchymal stem cells relieves knee osteoarthritis: Evidence from in vitro and in vivo studies DOI Creative Commons
Long Long, Guoyou Zou,

Yi Cheng

et al.

Journal of Orthopaedic Translation, Journal Year: 2023, Volume and Issue: 41, P. 20 - 32

Published: July 1, 2023

Synovial mesenchymal stem cell (SMSC) exerts chondroprotective effects in osteoarthritis (OA) clinical models. However, the regulatory potentials of SMSC-derived exosomes (SMSC-Exo) OA still need to be discovered, which attracted our attention.

Language: Английский

Citations

16
Immune Dysregulation and Cellular Composition in Lichen Sclerosus Revealed by Integrative Epigenetic Analysis with Cell Type Deconvolution DOI Creative Commons
Jianwei Wang,

Hailang Fan,

Zhengqing Bao

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 283 - 299

Published: Jan. 1, 2025

Lichen sclerosus (LS) is a chronic inflammatory disease affecting skin and mucosal tissues, particularly external genitalia, with risk of cancer. Its etiology unknown, possibly involving immune dysregulation inflammation. Study used DNA methylation (DNAme) single-cell RNA sequencing (scRNA-seq) to compare LS normal skin. A detailed DNAme profile was created, analyzing differentially methylated probes (DMPs) cell type-specific DMPs. EpiSCORE deconvolution infiltration analyses identified altered types in LS. Immunohistochemistry confirmed cellular changes. Enrichment analysis significantly pathways, communication described interactions among patterns generally distinguished from skin, few exceptions. Data showed that T cells increased fibroblasts decreased Immunohistochemical staining the changes cells. DMPs indicated significant impacts on fibroblast-related processes key pathways. The COLLAGEN signal most prominent communication. CD99-CD99 interaction strongest between fibroblasts. Combining scRNA-seq data revealed composition pathways LS, enhancing understanding its pathogenesis highlighting potential therapeutic targets diagnostic markers.

Language: Английский

Citations

0
In Silico Screening of Phytochemicals as Potential Inhibitors of the JAK/STATs Pathway in Psoriasis DOI

Lokendra Singh Rathor,

Divya Sahu,

Manju Rawat Singh

et al.

Assay and Drug Development Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

Citations

0
Exploring the therapeutic promise of fisetin: molecular mechanisms and clinical aspects in lung cancer DOI

Kavita Goyal,

M. Arockia Babu, Muhammad Inam Afzal

et al.

Journal of Complementary and Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract Fisetin, a flavonol belonging to the flavonoid subclass, is ubiquitous dietary present in fruits and vegetables, including fruit peels, has proven potential for anticancer activity, especially lung cancer – leading cause of cancer-related deaths globally. The current paper provides most detailed elaborate list various roles fisetin experimentally induced cells, these include promotion apoptosis, inhibition cell proliferation, migration, invasion, as well regulation autophagy. Among molecular targets, some identified pathways, such PI3K/Akt, MAPK, NF-κB, that affects are crucial tumor formation, so it can be considered chemopreventive agent. Moreover, improves effectiveness conventional treatments chemo- radiosensitizer minimizes side effects. However, overall utility clinical use now somewhat restricted by its poor solubility short half-life. It predicted future development nanotechnologies drug delivery, nanoparticle encapsulation, might help solve difficulties. Further Preclinical investigations required uniformly determine safety, efficacy, standard dosage consumption therapy.

Language: Английский

Citations

0
Fisetin alleviates Salmonella typhimurium-induced colitis through the TLR2/TLR4-NF-κB pathway, regulating microbiota, and repressing intracellular bacterial proliferation by focal adhesion kinase DOI
Jun Li, Yang Yang, Xinyu Zhang

et al.

European Journal of Nutrition, Journal Year: 2025, Volume and Issue: 64(3)

Published: March 19, 2025

Language: Английский

Citations

0
The Importance and Key Point of TCM Prevention and Control of Psoriasis DOI

建刚 周

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 2059 - 2064

Published: Jan. 1, 2025

Language: Английский

Citations

0
CRISPR/Cas9-Mediated Knockout and Overexpression Studies Unveil the Role of PD-L1 in Immune Modulation in a Psoriasis-like Mouse Model DOI Creative Commons

Chunjie Gao,

Yunxi Cai,

Xinxin Wu

et al.

Inflammation, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

The role of programmed death-ligand 1 (PD-L1), an essential immune checkpoint protein, has garnered considerable interest in recent years due to its influence on responses, particularly inhibiting immature Th cells into Th17 cells. This study aims examine the effect PD-L1 psoriasis progress, which is condition characterized by response dominated We constructed knockout (PD-L1KO) and overexpression (PD-L1OE) mice through CRISPR/Cas9 technology assess impact imiquimod (IMQ)-induced psoriasis-like mouse model. In comparison IMQ, ear thickness exhibited a reduction, PASI score decreased, HE sections revealed thinning epidermal spines PD-L1OE mice. PD-L1KO mice, however, showed opposite results. Moreover, immunohistochemical assessments skin lesion tissues demonstrated heightened proliferation inflammatory infiltration group, accompanied elevated tissue expression proliferating cell nuclear antigen (PCNA), Nuclear factor kappa-light-chain-enhancer activated B (NF-κB) p50, F4/80 IMQ-treated WT absence IMQ-induced was found intensify response, as evidenced phosphorylated signal transducers activators transcription 3 (pSTAT3) CD3 affected compared both According our findings, plays important roles inflammation, proliferation, regulating responses. Targeting may present promising therapeutic strategy for management psoriasis.

Language: Английский

Citations

0
Effects of shikonin and fisetin on dapsone metabolism in vitro and in vivo DOI Creative Commons

Hualu Wu,

Hongchang Fu, Jun Wu

et al.

Arabian Journal of Chemistry, Journal Year: 2025, Volume and Issue: 0, P. 1 - 7

Published: April 16, 2025

Language: Английский

Citations

0