Diagnostic Microbiology and Infectious Disease, Journal Year: 2022, Volume and Issue: 105(2), P. 115860 - 115860
Published: Nov. 12, 2022
Language: Английский
Vaccines, Journal Year: 2024, Volume and Issue: 12(7), P. 795 - 795
Published: July 18, 2024
Immunity against respiratory pathogens is often short-term, and, consequently, there an unmet need for the effective prevention of such infections. One infectious disease coronavirus 19 (COVID-19), which caused by novel Beta SARS-CoV-2 that emerged around end 2019. The World Health Organization declared illness a pandemic on 11 March 2020, and since then it has killed or sickened millions people globally. development COVID-19 systemic vaccines, impressively led to significant reduction in severity, hospitalization, mortality, contained pandemic’s expansion. However, these vaccines have not been able stop virus from spreading because restricted mucosal immunity. As result, breakthrough infections frequently occurred, new strains emerging. Furthermore, will likely continue circulate like influenza virus, co-exist with humans. upper tract nasal cavity are primary sites infection thus, mucosal/nasal vaccination induce response virus’ transmission warranted. In this review, we present status both approved those under evaluation clinical trials. our approach B-cell peptide-based applied prime-boost schedule elicit
Language: Английский
Citations
5
Frontiers in Medicine, Journal Year: 2022, Volume and Issue: 9
Published: Aug. 22, 2022
While all efforts have been undertaken to propagate the vaccination and develop remedies against SARS-CoV-2, no satisfactory management of this infection is available yet. Moreover, poor availability any preventive treatment measures SARS-CoV-2 in economically disadvantageous communities aggravates course pandemic. Here, we studied a new immunomodulatory phytotherapy (IP), an extract blackberry, chamomile, garlic, cloves, elderberry as potential low-cost solution for these problems given reported efficacy herbal medicine during previous SARS virus outbreak. The key feature infection, excessive inflammation, was vitro vivo assays under application IP. First, changes tumor-necrosis factor (TNF) lnteurleukin-1 beta (IL-1β) concentrations were measured culture human macrophages following lipopolysaccharide (LPS) challenge with IP or prednisolone. Second, chronically IP-pre-treated CD-1 mice received agonist Toll-like receptors (TLR)-7/8 resiquimod examined lung spleen expression pro-inflammatory cytokines blood formula. Finally, challenged LPS injection "sickness" behavior. Additionally, analyzed using high-potency-liquid chromatography (HPLC)-high-resolution-mass-spectrometry (HRMS). LPS-induced release TNF IL-1β reduced by both treatments. IP-treated displayed blunted over-expression SAA-2, ACE-2, CXCL1, CXCL10 decreased formula response resiquimod. injected showed normalized locomotion, anxiety, exploration behaviors but not abnormal forced swimming. Isoquercitrin, choline, leucine, chlorogenic acid, other constituents identified HPLC-HRMS likely underlie effects. Herbal IP-therapy decreases inflammation and, partly, "sickness behavior," suggesting its potency combat first via
Language: Английский
Citations
20
Healthcare, Journal Year: 2024, Volume and Issue: 12(2), P. 162 - 162
Published: Jan. 10, 2024
Introduction: The COVID-19 pandemic caused an international health emergency situation where nursing took on a fundamental role. high number of patients in hospital ICUs led to shift nurses’ working conditions and workload. Objective: objective this study was describe the experiences nurses who worked during acute phase pandemic. Methodology: A qualitative, descriptive carried out, with participation 21 ICU Data collection place between May July 2021 through in-depth interviews. Results: Three main themes emerged: (1) ICUs: frontline. (2) United against adversity: teamwork. (3) New optics critical care profession. perceived harshness, lack knowledge about virus generated confusion, anxiety fear due risk transmission family members relatives. marked management human, material economic resources. Novice learned at accelerated pace, significant physical psychological strain. Expert burden training new nurses. Although there were tense situations, experiencing these adverse situations as team feelings increased belonging, togetherness professional bonding for While participants noted increase motivation continue their profession, they also had feeling not having been cared deserve by healthcare institutions.
Language: Английский
Citations
4
Avicenna Journal of Medical Biotechnology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 25, 2025
Background: Since the initial outbreak, SARS-CoV-2 virus has continued to circulate and mutate, resulting in emergence of new viral sublineages. Due lack effective protection therapeutic measures against these variants, is able further evolve diversify. This study aimed screen a phage antibody library identify monoclonal antibodies single-chain variable fragment (scFv) format that target Receptor Binding Domain (RBD) different strains. The newly discovered scFv potential for use as diagnostic or option SARS-CoV-2. Methods: RBD protein was produced, purified, used an antigen during bio-panning. Six rounds panning enriched RBD-specific phages binding affinity binders were monitored by polyclonal ELISA. Subsequently, ELISA employed specific binders. After sequence confirmation, reactivity isolated anti-RBD evaluated. Additionally, bioinformatics tools determined interaction between selected Results: analysis demonstrated expressed retains its structural integrity effectively interacts with present sera COVID-19 patients. Through screening display library, strong-binding discovered, which can neutralize novel variants. Conclusion: findings this have led discovery neutralizes strains, offering immense research therapy purposes.
Language: Английский
Citations
0
Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
Bioinformation, Journal Year: 2022, Volume and Issue: 18(10), P. 908 - 911
Published: Oct. 31, 2022
Patients sero-positive for the Systemic Acute Respiratory Syndrome Corona virus2 (SARS-CoV2) virus develop Virus Disease 2019 (CoViD-19). CoViD-19 may be asymptomatic in some individuals, proffer mild symptoms other patients, and can a serious even lethal disease sub-group of population. The variables that determine severity have not been fully characterized. What is clear patients who survive return to state sero-negativity SARS-CoV2 generally within 3-5 weeks. However, several cases repeated infection reported, large proportion CoViD-19-recovered manifest multi-system multi-organ symptomatic pathologies weeks-to-months after resuming SARS-CoV2. This new pathological condition, originally termed Long Covid, now recognized as Post (PACS). original principal clusters signs PACS: likelihood relapse reinfection, physical fatigue cognitive slowdown, actually broadened include immune deregulation, cardiovascular coagulation abnormalities. development evaluation improved clinical interventions PACS are critical timely.
Language: Английский
Citations
13
Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(14), P. 5535 - 5546
Published: March 30, 2023
Cell entry of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) depends on specific host cell proteases, which are the key targets for preventing and treating viral infections. Herein, we describe miyabenol C trans-ε-viniferin, two resveratrol oligomers that specifically inhibit SARS-CoV-2 by targeting protease cathepsin L. Several cell-based assays were used to demonstrate effect oligomers, their target was identified via screening antiviral targets. Molecular docking analysis suggested could occupy active cavity The surface plasmon resonance assay showed equilibrium dissociation constant (KD) values C-cathepsin L trans-ε-viniferin-cathepsin 5.54 8.54 μM, respectively, indicating excellent binding ability Our study demonstrated potential application as lead compounds in controlling infection
Language: Английский
Citations
8
British Journal of Pharmacology, Journal Year: 2022, Volume and Issue: 180(1), P. 5 - 24
Published: Oct. 5, 2022
Epigenetic modifications have received increasing attention and been shown to be extensively involved in kidney development disease progression. Among them, the most common RNA modification, N6 -methyladenosine (m6 A), has dynamically reversibly exert its functions multiple ways, including splicing, export, decay translation initiation efficiency regulate mRNA fate. Moreover, m6 A also reported biological effects by destabilizing base pairing modulate various of RNAs. Most importantly, an number diseases, such as renal cell carcinoma, acute injury chronic disease, found associated with aberrant patterns. In this review, we comprehensively review critical roles diseases discuss possibilities relevance A-targeted epigenetic therapy, integrated comprehensive description detailed alterations specific loci that contribute cellular processes are diseases.
Language: Английский
Citations
12
Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12
Published: July 22, 2022
The world has seen the emergence of a new virus in 2019, SARS-CoV-2, causing COVID-19 pandemic and millions deaths worldwide. Microscopy can be much more informative than conventional detection methods such as RT-PCR. This review aims to present up-to-date microscopy observations patients, vitro studies viral proteins their interaction with host, discuss techniques for study summarize reagents used SARS-CoV-2 detection. From basic fluorescence high resolution combined technologies, this article shows power potential techniques, especially field virology.
Language: Английский
Citations
5
Current Oncology, Journal Year: 2022, Volume and Issue: 29(5), P. 3364 - 3374
Published: May 6, 2022
Background: Information on immune responses in cancer patients following mRNA COVID-19 vaccines is still insufficient, but generally, had impaired serological responses, especially those with hematological malignancies. We evaluated response to vaccine receiving chemotherapy compared healthy controls. Methods: In total, 195 and 400 randomly selected controls who been administered a Pfizer-BioNTech or Moderna two doses were compared. The threshold of positivity was 4.33 BAU/mL. Patients anticancer treatment after the first second dose vaccines. Results: TOTAL OF 169 (87%) solid tumors 26 hemolymphopoietic diseases. Seropositivity rate lower than (91% vs. 96%), an age/gender-adjusted ratio (RR) 0.95 (95% CL = 0.89–1.02). Positivity found 97% cancers 50% tumors. Both advanced adjuvant therapy seemed slightly reduce seropositivity rates when (RR 0.97, 95% 0.89–1.06; RR 0.94, 0.87–1.01). Conclusions: vaccination similar affected by On contrary, hemolymphopietic show much
Language: Английский
Citations
5