Preliminary study based on methylation and transcriptome gene sequencing of lncRNAs and immune infiltration in hypopharyngeal carcinoma DOI Creative Commons

Kainan Wu,

Fen Chang,

Wenming Li

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: April 25, 2023

Background Hypopharyngeal squamous cell cancer (HSCC) is one of the most malignant tumors head and neck. It not easy to detect in early stage due its hidden location; thus, lymph node metastasis highly likely at diagnosis, leading a poor prognosis. believed that epigenetic modification related invasion metastasis. However, role m6A-related lncRNA tumor microenvironment (TME) HSCC remains unclear. Methods The whole transcriptome methylation sequencing 5 pairs tissues adjacent were performed identify profiles lncRNAs. biological significance lncRNAs differentially expressing m6A peak was analyzed by Gene Ontology Kyoto Encyclopedia Genes Genomes. By constructing an lncRNA-microRNA network, mechanism analyzed. relative expression levels selected examined quantitative polymerase chain reaction. CIBERSORT algorithm used evaluate proportion immune infiltration paracancerous tissues. Results Based on in-depth analysis results, 14413 expressed revealed, including 7329 up-regulated 7084 down-regulated Additionally, 4542 up-methylated 2253 down-methylated detected. We demonstrated patterns gene transcriptome. In intersection methylated lncRNAs, 51 with 40 screened, significantly differentiated further studied. analysis, B memory elevated tissue, while γδT amount decreased. Conclusion might be involved pathogenesis. Infiltration cells provide new direction for treatment. This study provides insights exploring possible pathogenesis searching potential therapeutic targets.

Language: Английский

Long non‐coding RNA as a potential diagnostic and prognostic biomarker in melanoma: A systematic review and meta‐analysis DOI Creative Commons
Mahdi Masrour, Shaghayegh Khanmohammadi, Parisa Fallahtafti

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(3)

Published: Jan. 9, 2024

Abstract Recently, long noncoding RNAs (lncRNAs) have been applied as biomarkers for melanoma patients. In this systematic review and meta‐analysis, we investigated the diagnostic prognostic value of lncRNAs. We used keywords ‘lncRNA’ ‘melanoma’ to search databases studies published before June 14th, 2023. The specificity, sensitivity AUC were utilized assess accuracy was assessed using overall survival, progression‐free survival disease‐free hazard ratios. After screening 1191 articles, included seven in evaluation section 17 prognosis section. Reitsma bivariate model estimated a cumulative 0.724 (95% CI: 0.659–0.781, p < 0.001) specificity 0.812 0.752–0.859, 0.001). pooled 0.780 0.749–0.811, 0.0001). HR 2.723 2.259–3.283, Two reported an less than one, with 0.348 0.200–0.607, 0.0002). 2.913 2.050–4.138, Four 0.457 0.256–0.817). 2.760 2.009–3.792, conclusion, expression lncRNAs patients affects prognosis. LncRNAs can also be employed biomarkers.

Language: Английский

Citations

9
Genetics and RNA Regulation of Uveal Melanoma DOI Open Access
Cristina Barbagallo, Michele Stella, Giuseppe Broggi

et al.

Cancers, Journal Year: 2023, Volume and Issue: 15(3), P. 775 - 775

Published: Jan. 26, 2023

Uveal melanoma (UM) is the most common intraocular malignant tumor and frequent not affecting skin. While rate of UM occurrence relatively low, about 50% patients develop metastasis, primarily to liver, with lethal outcome despite medical treatment. Notwithstanding that etiopathogenesis still under investigation, a set known mutations chromosomal aberrations are associated its pathogenesis have relevant prognostic value. The frequently mutated genes BAP1, EIF1AX, GNA11, GNAQ, SF3B1, mutually exclusive occurring in GNAQ almost ones BAP1 EIF1AX. Among aberrations, monosomy chromosome 3 frequent, followed by gain 8q, full or partial loss chromosomes 1 6. In addition, epigenetic mechanisms regulated non-coding RNAs (ncRNA), namely microRNAs long RNAs, also been investigated. Several papers investigating role ncRNAs reported their dysregulated expression affects cancer-related processes both vitro vivo models. This review will summarize current findings genetic mutations, ncRNA dysregulation establishing biology.

Language: Английский

Citations

22
1,4-dihydroxy quininib activates ferroptosis pathways in metastatic uveal melanoma and reveals a novel prognostic biomarker signature DOI Creative Commons
Valentina Tonelotto, Marcel Costa-García,

Eve O’Reilly

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Feb. 10, 2024

Uveal melanoma (UM) is an ocular cancer, with propensity for lethal liver metastases. When metastatic UM (MUM) occurs, as few 8% of patients survive beyond two years. Efficacious treatments MUM are urgently needed. 1,4-dihydroxy quininib, a cysteinyl leukotriene receptor 1 (CysLT

Language: Английский

Citations

5
From diagnosis to therapy: The transformative role of lncRNAs in eye cancer management DOI
Ali Hazazi,

Abdulmajid A. AlShehah,

Farhan R. Khan

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 254, P. 155081 - 155081

Published: Jan. 3, 2024

Language: Английский

Citations

4
A novel defined risk signature of ferroptosis-related lncRNAs for predicting prognosis, immune infiltration, and chemotherapy response in multiple myeloma DOI Creative Commons
Wei Yu,

Zizi Jing,

Jialin Tang

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 11, 2025

Language: Английский

Citations

0
Rediscovery of PHI-1/PPP1R14B: Emerging Roles of Cellular PP1 Signaling Mediated by the PPP1R14B Gene Product in Multiple Cancers and Beyond DOI Creative Commons
Masumi Eto

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 344 - 344

Published: Feb. 27, 2025

PHI-1, encoded by PPP1R14B, regulates cellular protein phosphatase-1 (PP1) signaling and has emerged as both a biomarker therapeutic target. Initially identified phospholipase-neighboring gene (PNG), PHI-1 is now known for its phosphorylation-dependent inhibition of PP1 holoenzymes, with bi-directional roles depending on expression levels. Under physiological conditions, selectively activity to maintain homeostasis, whereas pathological upregulation promotes oncogenic pathways, stabilizes tumor-promoting proteins, modulates immune responses. This article explores PHI-1’s emerging role pan-cancer in parallel emphasizing functions networks, smooth muscle contraction, cytoskeletal dynamics, selective proteostasis. The mechanistic insights highlight potential precision oncology, offering opportunities developing diagnostics therapies that target conditional functions.

Language: Английский

Citations

0
N6-Methyladenosine regulator RBM15B acts as an independent prognostic biomarker and its clinical significance in uveal melanoma DOI Creative Commons
Tianyu Wang,

Jianhao Bai,

Yuanyuan Zhang

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 8, 2022

Uveal melanoma (UM) is the most frequent intraocular malignant tumor in adults. N 6 -Methyladenosine (m A) methylation recognized as critical epigenetic change and implicated development of many malignancies. However, its prognostic value UM poorly understood. RNA-seq clinical data from The Cancer Genome Atlas (TCGA) help us better understand relationship between m A regulators patients. Herein, four groups established by consensus clustering were shown to have different immune cell infiltrations survival. Five regulators, including RBM15B, IGF2BP1, IGF2BP2, YTHDF3, YTHDF1, associated with prognosis Intriguingly, RBM15B was confirmed be only independent factor for it significantly correlated clinicopathologic characteristics UM. Notably, expression negatively checkpoints. Furthermore, LINC00665/hsa-let-7b-5p/RBM15B axis LINC00638/hsa-miR-103a-3p/RBM15B found potential biomarkers In a nutshell, this work, through bioinformatics analysis, systematically described gene signatures values regulators. an protective factor, which may crosstalk within

Language: Английский

Citations

15
Methylation-driven mechanisms of allergic rhinitis during pollen and non-pollen seasons using integrated bioinformatics analysis DOI Creative Commons
Pengcheng Sun, Yì Wáng, Xing Liu

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: April 18, 2024

Background: Allergic rhinitis (AR) is a widespread allergic airway disease that results from complex interplay between genetic and environmental factors affects approximately 10%–40% of the global population. Pollen common allergen, exposure to pollen can cause epigenetic changes. However, mechanism underlying pollen-induced DNA methylation changes their potential effects on march are still unclear. The purpose this study was explore methylation-driven mechanisms AR during non-pollen seasons using bioinformatics analysis investigate relationship with asthma. Methods: We downloaded gene expression data GEO database (GSE50387: GSE50222, GSE50101) identified differentially methylated positions (DMPs) expressed genes (DEGs) CHAMP limma packages. Through correlation analysis, we performed pathway enrichment annotate functions. incorporated external combined asthma (GSE101720) for identify key CpGs promote transformation also utilized olive allergy (GSE54522) validate genes. Weighted network (WGCNA) employed modules significantly correlated allergy. extracted related ZNF667-AS1 significant module intelligent clustering KOBAS-i. set function . Results: 20 24 CpG-Gene pairings seasons. After incorporating GSE101720, found may facilitate into season. This finding further validated in another dataset, GSE54522, which associated WGCNA 17 modules, among blue showed allergies. located module. prominent cluster pathways KOBAS-i results, including Toll-like receptor ( TLR ) family, MyD88 , MAPK oxidative stress. Gene around cg05508084 (paired indicated its involvement initiating modulating inflammation perspective signaling. Conclusion: patients due exposure. provides new insights molecular

Language: Английский

Citations

2
Ferroptosis induced by DCPS depletion diminishes hepatic metastasis in uveal melanoma DOI Creative Commons
Bei Jin, Yang Luo,

Qianyun Ye

et al.

Biochemical Pharmacology, Journal Year: 2023, Volume and Issue: 213, P. 115625 - 115625

Published: May 26, 2023

Hepatic metastasis develops in ∼50% of uveal melanoma (UM) patients with scarcely effective treatment resulting lethality. The underlying mechanism liver remains elusive. Ferroptosis, a cell death form characterized by lipid peroxide, cancer cells may decrease metastatic colonization. In the present study, we hypothesized that decapping scavenger enzymes (DCPS) impact ferroptosis regulating mRNA decay during colonization UM to liver. We found inhibition DCPS shRNA or RG3039 induced gene transcript alteration and through reducing turnover GLRX. Ferroptosis eliminates stem-like UM. Inhibition hampered growth proliferation both vitro vivo. Furthermore, targeting diminished hepatic cells. These findings shed light on understanding DCPS-mediated pre-mRNA metabolic pathway which disseminated gain enhanced malignant features promote metastasis, providing rational target for

Language: Английский

Citations

6
A comprehensive analysis of FBN2 in bladder cancer: A risk factor and the tumour microenvironment influencer DOI Creative Commons
Zechao Lu, Zeguang Lu, Yongchang Lai

et al.

IET Systems Biology, Journal Year: 2023, Volume and Issue: 17(4), P. 162 - 173

Published: June 19, 2023

Abstract Bladder cancer (BLCA) is a common and difficult‐to‐manage disease worldwide. Most type of BLCA urothelial carcinoma (UC). Fibrillin 2 (FBN2) was first discovered while studying Marfan syndrome, its encoded products are associated with elastin fibres. To date, the role FBN2 in remains unclear. The authors downloaded data from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO). patients were divided into high expression low groups, survival curve, clinical characteristics, tumour microenvironment (TME), immune cell differences analysed between two groups. Then, differentially expressed genes (DEGs) filtered, functional enrichment for DEGs performed. Finally, chemotherapy drug susceptibility analysis based on groups conducted. found upregulated proved that could be an independent prognostic factor BLCA. TME showed affects several aspects TME. stromal score, which may lead to immunosuppression detrimental immunotherapy. In addition, NK cells resting, macrophage M0 infiltration, other phenomena infiltration appeared group FBN2. related sensitivity some drugs. systematically investigated effects mechanisms provided new understanding as risk influencer

Language: Английский

Citations

5