Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: March 21, 2024
Abstract
The
corneal
epithelium
acts
as
a
barrier
to
pathogens
entering
the
eye;
epithelial
cells
are
continuously
renewed
by
uni-potent,
quiescent
limbal
stem
(LSCs)
located
at
limbus,
where
cornea
transitions
conjunctiva.
There
has
yet
be
consensus
on
LSC
markers
and
their
transcriptome
profile
is
not
fully
understood,
which
may
due
using
cadaveric
tissue
without
an
intact
cell
niche
for
transcriptomics.
In
this
study,
we
addressed
problem
single
nuclei
RNA
sequencing
(snRNAseq)
healthy
human
that
was
immediately
snap-frozen
after
excision
from
patients
undergoing
cataract
surgery.
We
identified
LSCs
sub-population
of
with
low
level
total
transcript
counts.
Moreover,
TP63,
KRT15,
CXCL14,
ITGβ4
were
found
highly
expressed
in
transiently
amplifying
(TACs),
constitute
progenitor
populations
limbus.
surface
SLC6A6
could
used
enrich
progenitors,
also
specifically
express
putative
MMP10
AC093496.1.
Aggregate,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 6, 2024
Abstract
Diabetes
significantly
impairs
the
body's
wound‐healing
capabilities,
leading
to
chronic,
infection‐prone
wounds.
These
wounds
are
characterized
by
hyperglycemia,
inflammation,
hypoxia,
variable
pH
levels,
increased
matrix
metalloproteinase
activity,
oxidative
stress,
and
bacterial
colonization.
complex
conditions
complicate
effective
wound
management,
prompting
development
of
advanced
diabetic
care
strategies
that
exploit
specific
characteristics
such
as
acidic
pH,
high
glucose
stress
trigger
controlled
drug
release,
thereby
enhancing
therapeutic
effects
dressings.
Among
solutions,
hydrogels
emerge
promising
due
their
stimuli‐responsive
nature,
making
them
highly
for
managing
these
The
latest
advancements
in
mono/multi‐stimuli‐responsive
smart
showcase
superiority
potential
healthcare
materials,
highlighted
relevant
case
studies.
However,
traditional
dressings
fall
short
meeting
nuanced
needs
wounds,
adjustable
adhesion,
easy
removal,
real‐time
status
monitoring,
dynamic
release
adjustment
according
wound's
conditions.
Responsive
represent
a
significant
leap
forward
proficient
sensing
responding
environment,
offering
more
targeted
approach
treatment.
This
review
highlights
recent
dressing,
delivery,
emphasizing
role
improving
healing.
It
addresses
ongoing
challenges
future
directions,
aiming
guide
clinical
adoption.
Journal of Personalized Medicine,
Journal Year:
2025,
Volume and Issue:
15(1), P. 14 - 14
Published: Jan. 2, 2025
Background/Objectives:
There
is
a
growing
interest
in
the
research
of
wound
healing
mechanisms
worldwide.
Particular
attention
has
been
paid
to
expression
tissue
remodeling-
and
inflammation-related
factors.
Herein,
we
investigate
patterns
TGF-β1,
IL-6,
TNF-a,
uPA,
uPA
receptors,
MMP-2,
MMP-9
through
four
phases
normal
wound-healing
process
humans.
Methods:
Twenty-two
individuals
presenting
with
first
episode
pilonidal
sinus
underwent
surgical
excision
was
left
heal
by
secondary
intention.
Sequential
biopsies
were
collected
on
day
0
(operation),
2
(inflammatory
phase),
9
(proliferative
14
(tissue
remodeling
phase).
The
levels
proteins
evaluated
using
reverse
transcription-quantitative
PCR.
Statistical
analyses
performed
GraphPad
Prism
software.
One-way
analysis
variance
(ANOVA)
Dunn's
Multiple
Comparison
post
hoc
test
used.
Results:
results
showed
statistically
significant
differences
expressions
factors
during
(p
<
0.05).
TGF-b1
increased
days
9.
TNF-a
then
decreased
Il-6
14.
uPa
mRNA
up
but
its
receptor
exhibited
high
throughout
observation
time.
Finally,
MMP-2
declined
14,
while
highly
expressed
until
14th
postoperative
day.
Conclusions:
Each
factor
investigated
this
study
an
important
distinct
role
repair
procedure.
Further
investigation
required
evaluate
tissue-specific
regulation
these
their
potential
use
as
therapeutic
targets
or
prognostic
biomarkers
healing.
Journal of Theoretical Biology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 112083 - 112083
Published: Feb. 1, 2025
Understanding
the
biochemistry
and
pharmacodynamics
of
chronic
wounds
is
key
importance,
due
to
millions
people
in
UK
affected
significant
cost
NHS.
Chronic
are
characterised
by
elevated
concentrations
matrix
metalloproteinases
(MMPs)
that
destroy
surrounding
extracellular
(ECM).
However,
fibroblasts
can
produce
tissue
inhibitors
MMPs
(TIMPs)
order
regulate
wound
healing.
Therefore,
role
TIMPs
both
acute
needs
be
properly
understood
develop
therapeutic
treatments.
In
this
work,
we
propose
a
reaction-diffusion
system
four
partial
differential
equations
describe
interaction
ECM,
fibroblasts,
MMPs,
wound.
We
observe
that,
subject
parameter
sets
corresponding
healing,
mathematical
model
gives
rise
travelling
wave
solutions.
Using
bifurcation
analysis,
demonstrate
excessive
degradation
ECM
results
emergence
wounds,
reversal
these
prohibited
for
lower
TIMP
production
values.
These
replicated
within
simplified
obtained
via
sensitivity
analysis.
This
further
extended
more
realistic
spatial
domains
where
effectiveness
hydrogel
containing
as
treatment
wounds.
Microscopy Research and Technique,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 27, 2025
ABSTRACT
The
current
study
assessed
the
wound
healing
potential
of
Bergenia
ciliata
(BC)
extract,
BC‐loaded
Salvia
hispanica
hydrogel
(CH‐BC)
and
nanoparticles
(NPs)–loaded
(CH‐BC
AgNPs/NPs‐loaded
hydrogel)
using
in
vitro
vivo
studies.
prepared
its
components
were
analyzed
UV–visible
spectrophotometry
(UV–vis),
scanning
electron
microscopy
(SEM),
dynamic
light
scattering
(DLS)
analysis/particle
size
analyzer
(PSA),
zeta
potential,
fourier
transform
infrared
spectrometry
(FT‐IR),
x‐ray
diffraction
(XRD).
Peaks
peak
shifts
at
450–460,
250–260,
270–280
nm
UV–vis
FT‐IR
(500–4000
cm
−1
)
confirmed
extract
loading
various
functional
group
presence.
DLS
nanometric
BC
AgNP
while
indicated
slightly
negative
charge
hydrogel.
SEM
average
topography
–mediated
(BC
AgNPs)
AgNPs
loaded
AgNPs),
XRD
peaks
2ϴ
(10–70)
topographical
crystalline
porous
nature
materials.
showed
higher
radical
scavenging
(74.19%
±
1.84%)
iron
chelation
activity
(93.70
2.20)
50
25
μL/mL,
respectively,
CH‐BC
produced
lowest
(48.97%
3.0%)
75
μL/mL
(54.35
3.24).
Moreover,
Chia
(CH)
synergistically
enhanced
(1.30%)
DPPH
scavenging.
contraction
percentage
augmented
as
potent
candidate
for
(14th
day).
These
findings
further
supported
by
a
significant
(
p
<
0.001)
restoration
MMP2
(184.6
11.7
pg/mL),
TIMPs
(184.8
15.7
GPx
(153.4
11.5
IL‐6
(10
1
pg/mL)
levels
compared
to
those
diabetic
control.
normal
reepithelization,
angiogenesis,
maturation
wounds
treatment
groups
after
histological
analysis
strengthened
supposed
rationale
that
CH,
along
with
AgNPs,
can
act
improve
therapeutic
results.
Hence,
appeared
sustainable,
biocompatible,
nontoxic
agent
paved
way
futuristic
biomedical
investigations.
Gels,
Journal Year:
2023,
Volume and Issue:
9(11), P. 867 - 867
Published: Oct. 30, 2023
Diabetes
is
a
condition
correlated
with
high
number
of
diagnosed
chronic
wounds
as
result
complex
pathophysiological
mechanism.
Diabetic
are
characterized
by
disorganized
and
longer
stages,
compared
to
normal
wound
healing.
Natural
polymer
hydrogels
can
act
good
dressings
due
their
versatile
physicochemical
properties,
represented
mainly
water
content
biocompatibility.
bioactive
polymers
loaded
compounds
providing
antibacterial
antioxidant
modulation
inflammation
adherence
wounded
tissue,
traditional
dressings,
which
enables
promising
future
applications
for
diabetic
compounds,
such
polyphenols,
polysaccharides
proteins
have
great
advantages
in
promoting
healing
diabetes
antioxidant,
anti-inflammatory,
antimicrobial,
anti-allergic
properties.
The
present
paper
aims
review
the
mechanisms
underlining
main
issues
those
specifically
occurring
diabetes.
Also,
highlights
recent
state
art
related
effect
enriched
natural
developed
biocompatible
functional
materials
improving
diabetic-related
novel
therapeutic
strategies
that
could
prevent
limb
amputation
increase
quality
life
patients.
International Wound Journal,
Journal Year:
2024,
Volume and Issue:
21(2)
Published: Feb. 1, 2024
This
article
describes
the
contemporary
bioengineering
theory
and
practice
of
evaluating
fluid
handling
performance
foam-based
dressings,
with
focus
on
important
clinically
relevant
engineering
structure-function
relationships
advanced
laboratory
testing
methods
for
pre-clinical
quantitative
assessments
this
common
type
wound
dressings.
The
effects
key
dressing
material-related
treatment-related
physical
factors
absorbency
overall
dressings
are
thoroughly
quantitively
analysed.
Discussions
include
exudate
viscosity
temperature,
action
mechanical
forces
microstructure
associated
interactions.
Based
comprehensive
review,
we
propose
a
newly
developed
method,
experimental
metrics
clinical
benchmarks
that
can
set
standard
robust
evaluations.
purpose
evaluative
framework
is
to
translate
characteristics
determinants
foam
into
achievable
best
outcomes.
These
guiding
principles
distinguishing
desirable
properties
contribute
optimal
in
settings.
The International Journal of Lower Extremity Wounds,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 4, 2024
Chronic
wounds
remain
a
significant
clinical
challenge
both
for
those
affected
and
healthcare
systems.
The
treatment
is
often
comprised
complex.
All
patients
should
receive
wound
care
that
integrated
into
holistic
approach
involving
local
management
addresses
the
underlying
etiology
provides
gold
standard
therapy
to
support
healing,
avoid
complications
be
more
cost
effective.
There
have
been
advances
in
medicine
over
last
few
decades.
development
of
new
technologies
therapeutics
also
constantly
increasing.
To
help
standardize
practice
with
regard
multitude
products,
M.O.I.S.T.
concept
was
developed
by
multidisciplinary
expert
group.
M
stands
moisture
balance,
O
oxygen
I
infection
control,
S
supporting
strategies,
T
tissue
management.
Since
concept,
which
originated
German-speaking
countries,
now
intended
provide
professionals
an
adapted
instrument
used
practice,
recent
update
has
undertaken
group
interdisciplinary
experts
align
it
international
standards.
can
internationally
as
educational
tool
practical
implementation
modern
concepts
chronic
routine
practice.
The Journal of Physiology,
Journal Year:
2024,
Volume and Issue:
602(11), P. 2627 - 2648
Published: May 23, 2024
Homeostasis
constitutes
a
key
concept
in
physiology
and
refers
to
self-regulating
processes
that
maintain
internal
stability
when
adjusting
changing
external
conditions.
It
diminishes
entropy
constituting
driving
force
behind
evolution.
Natural
selection
might
act
on
homeostatic
regulatory
mechanisms
control
including
homeodynamics,
allostasis,
hormesis
homeorhesis,
where
different
stable
stationary
states
are
reached.
Regeneration
is
under
through
hormesis.
Damage
tissues
initiates
response
restore
the
impaired
equilibrium
caused
by
mild
stress
using
cell
proliferation,
differentiation
death
recover
structure
function.
Repair
homeorhetic
change
leading
new
state
with
decreased
functionality
fibrotic
scarring
without
reconstruction
of
3-D
pattern.
Mechanisms
determining
entrance
tissue
or
organ
regeneration
repair
include
balance
between
innate
adaptive
immune
cells
relation
plasticity
stromal
stem
responses,
redox
balance.
The
regenerative
reparative
capacities
vary
species,
distinct
organs,
at
stages
development
ageing.
Many
signals
pathways
play
crucial
roles
regulating
protein
synthesis,
cellular
growth,
inflammation,
autophagy,
lysosomal
function,
metabolism
metalloproteinase
signalling.
Attempts
favour
damaged
those
low
proliferative
rates
have
been
made;
however,
there
evolutionary
constraint
poor
proliferation
unfavourable
environments
tumour
development.
More
research
required
better
understand
these
mechanisms.
