Dysregulation of extracellular vesicle protein cargo in female ME/CFS cases and sedentary controls in response to maximal exercise DOI Open Access
Ludovic Giloteaux, Katherine A. Glass, Arnaud Germain

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 29, 2023

ABSTRACT In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue, reduces risk of chronic diseases. Although is suggested as a lifestyle intervention to manage various illnesses, it negatively affects people with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), who suffer from intolerance. We hypothesized that altered extracellular vesicle (EV) signaling in ME/CFS patients after an challenge may contribute their prolonged exacerbated negative response exertion (post-exertional malaise). EVs were isolated by size exclusion chromatography the plasma 18 female 17 age- BMI-matched sedentary controls at three time points: before, 15 minutes, 24 hours maximal cardiopulmonary test. characterized using nanoparticle tracking analysis protein cargo was quantified Tandem Mass Tag-based (TMT) proteomics. The results show EV proteome differently than individuals changes proteins are strongly correlated symptom severity ME/CFS. Differentially abundant vs. involved many pathways systems, including coagulation processes, contraction (both smooth skeletal muscle), cytoskeletal proteins, immune system, brain signaling.

Language: Английский

Cell Type-Specific Extracellular Vesicles and Their Impact on Health and Disease DOI Open Access
Sohil Amin,

Hamed Massoumi,

Deepshikha Tewari

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2730 - 2730

Published: Feb. 27, 2024

Extracellular vesicles (EVs), a diverse group of cell-derived exocytosed particles, are pivotal in mediating intercellular communication due to their ability selectively transfer biomolecules specific cell types. EVs, composed proteins, nucleic acids, and lipids, taken up by cells affect variety signaling cascades. Research the field has primarily focused on stem with particular focus mesenchymal cells, for potential therapeutic benefits. Recently, tissue-specific EVs or type-specific extracellular (CTS-EVs), have garnered attention unique biogenesis molecular composition because they enable highly targeted cell-specific communication. Various studies outlined roles that CTS-EVs play physiological function maintenance homeostasis, including immune modulation, tissue regeneration, organ development. These properties also exploited disease propagation, such as cancer, neurological disorders, infectious diseases, autoimmune conditions, more. The insights gained from analyzing different biological not only enhance our understanding pathogenesis but open new avenues innovative diagnostic biomarkers targets wide spectrum medical conditions. This review comprehensively outlines current CTS-EV origins, within normal physiology, implications diseased states.

Language: Английский

Citations

20
Proteomic profiling of mesenchymal stem cell‐derived extracellular vesicles: Impact of isolation methods on protein cargo DOI Creative Commons
Morteza Abyadeh, Shahab Mirshahvaladi, Sara Assar Kashani

et al.

Journal of Extracellular Biology, Journal Year: 2024, Volume and Issue: 3(6)

Published: June 1, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell‐to‐cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)‐derived obtained through different techniques: ultracentrifugation (UC), high‐speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS‐EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cell‐cell interactions extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Language: Английский

Citations

12
Dysregulation of extracellular vesicle protein cargo in female myalgic encephalomyelitis/chronic fatigue syndrome cases and sedentary controls in response to maximal exercise DOI Creative Commons
Ludovic Giloteaux, Katherine A. Glass, Arnaud Germain

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue reduces the risk of chronic diseases. Although is suggested as a lifestyle intervention to manage various illnesses, it negatively affects people with myalgic encephalomyelitis/chronic syndrome (ME/CFS), who suffer from intolerance. We hypothesized that altered extracellular vesicle (EV) signalling in ME/CFS patients after an challenge may contribute their prolonged exacerbated negative response exertion (post-exertional malaise). EVs were isolated by size exclusion chromatography plasma 18 female 17 age- BMI-matched sedentary controls at three time points: before, 15 min, 24 h maximal cardiopulmonary test. characterized using nanoparticle tracking analysis protein cargo was quantified Tandem Mass Tag-based (TMT) proteomics. The results show EV proteome differently than individuals changes proteins are strongly correlated symptom severity ME/CFS. Differentially abundant versus involved many pathways systems, including coagulation processes, contraction (both smooth skeletal muscle), cytoskeletal proteins, immune system brain signalling.

Language: Английский

Citations

7
Insights into optimizing exosome therapies for acute skin wound healing and other tissue repair DOI

Tianjing Sun,

Mo Li,

Qi Liu

et al.

Frontiers of Medicine, Journal Year: 2024, Volume and Issue: 18(2), P. 258 - 284

Published: Jan. 13, 2024

Language: Английский

Citations

6
Non-Clinical Safety Evaluation of Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells in Cynomolgus Monkeys DOI Creative Commons

Xinmei Hu,

Can-Can Wang,

Yu Xiao

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 4923 - 4939

Published: May 1, 2024

Purpose: In recent years, exosomes have been proved to be used treat many diseases. However, due the lack of uniform quality control standards for exosomes, safety is still a problem solved, especially now more and are in clinical trials, its non-clinical evaluation particularly important. there no standard at present. Therefore, this study will refer criteria therapeutic biological products, adopt non-human primates evaluate human umbilical cord mesenchymal stem cell from general pharmacology immunotoxicity, aiming establishing system providing reference application future. Methods: 3.85 × 10 12 derived cells were injected into cynomolgus monkeys intravenously. The changes conditions, hematology, immunoglobulin, Th1/Th2 cytokines, T lymphocytes B lymphocytes, immune organs observed before within 14 days after injection. Results: results showed that did not obvious pathological effects on blood, coagulation function, organ coefficient, major organs, (spleen, thymus, bone marrow) monkeys. number granulocyte-macrophage colonies group was significantly higher than group. Conclusion: To sum up, pharmacological immunotoxicity injection may adverse reactions This dose relatively safe treatment, which provides basis research reliable future exosomes. Keywords: monkeys, safety,

Language: Английский

Citations

5
Advances of mesenchymal stem cells and their derived extracellular vesicles as a promising therapy for acute respiratory distress syndrome: from bench to clinic DOI Creative Commons

Xiaoli Zhuang,

Yu Jiang, Xiaofang Yang

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 29, 2023

Acute respiratory distress syndrome (ARDS) is an acute inflammatory lung injury characterized by diffuse alveolar damage. The period prevalence of ARDS was 10.4% ICU admissions in 50 countries. Although great progress has been made supportive care, the hospital mortality rate severe still up to 46.1%. Moreover, now, there no effective pharmacotherapy for and most clinical trials focusing on consistently drugs have met disappointing results. Mesenchymal stem cells (MSCs) their derived extracellular vesicles (EVs) spawned intense interest a wide range researchers clinicians due robust anti-inflammatory, anti-apoptotic tissue regeneration properties. A growing body evidence from preclinical studies confirmed promising therapeutic potential MSCs EVs treatment ARDS. Based inspiring experimental results, designed evaluate safety efficacy patients. exploring optimal time window regimen drug administration are ongoing. Therefore, this review aims present overview characteristics mesenchymal EVs, mechanisms research that over past 5 years.

Language: Английский

Citations

9
Proteomic Profiling of Mesenchymal Stem Cell-Derived Extracellular Vesicles: Impact of Isolation Methods on Protein Cargo DOI Open Access
Morteza Abyadeh, Shahab Mirshahvaladi, Sara Assar Kashani

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell-to-cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)-derived obtained through different techniques: ultracentrifugation (UC), high-speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS-EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cellLJcell interactions, extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Language: Английский

Citations

2
Immunomodulatory potential of human clonal mesenchymal stem cells and their extracellular vesicle subpopulations in an inflammatory-mediated diabetic Rhesus monkey model DOI
Sara Assar Kashani,

Roghayeh Navabi,

Azadeh Amini

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 329, P. 121950 - 121950

Published: July 18, 2023

Language: Английский

Citations

6
Dysregulation of extracellular vesicle protein cargo in female ME/CFS cases and sedentary controls in response to maximal exercise DOI Open Access
Ludovic Giloteaux, Katherine A. Glass, Arnaud Germain

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 29, 2023

ABSTRACT In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue, reduces risk of chronic diseases. Although is suggested as a lifestyle intervention to manage various illnesses, it negatively affects people with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), who suffer from intolerance. We hypothesized that altered extracellular vesicle (EV) signaling in ME/CFS patients after an challenge may contribute their prolonged exacerbated negative response exertion (post-exertional malaise). EVs were isolated by size exclusion chromatography the plasma 18 female 17 age- BMI-matched sedentary controls at three time points: before, 15 minutes, 24 hours maximal cardiopulmonary test. characterized using nanoparticle tracking analysis protein cargo was quantified Tandem Mass Tag-based (TMT) proteomics. The results show EV proteome differently than individuals changes proteins are strongly correlated symptom severity ME/CFS. Differentially abundant vs. involved many pathways systems, including coagulation processes, contraction (both smooth skeletal muscle), cytoskeletal proteins, immune system, brain signaling.

Language: Английский

Citations

1