International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 137102 - 137102
Published: Oct. 31, 2024
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 855 - 855
Published: Jan. 20, 2025
Curcumin, a compound known for its antioxidant and neuroprotective properties, faces challenges due to low water solubility, which can limit effectiveness. One effective method address this issue is through amorphization. Incorporating curcumin into polymeric matrix form amorphous solid dispersions common approach. Another strategy involves co-amorphous systems, where low-molecular-weight components act as co-formers. A recent innovative approach combines these strategies. This study used tryptophan co-former prepared systems using supercritical fluid technology. The nature of two was confirmed X-ray powder diffraction: one with 10% polymer, another curcumin, tryptophan. Fourier-transform infrared analysis demonstrated molecular interactions among all in the systems. Scanning electron microscopy revealed that amorphization process significantly modified morphology particles. ternary system notably increased solubility by over 300-fold. both exhibited higher dissolution rates compared crystalline form. showed more than threefold improvement permeability according PAMPA test. enhanced led sixfold increase activity 25-fold inhibition enzyme butyrylcholinesterase.
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6099 - 6099
Published: June 1, 2024
Michael acceptors represent a class of compounds with potential anti-cancer properties. They act by binding to nucleophilic sites in biological molecules, thereby disrupting cancer cell function and inducing death. This mode action, as well their ability be modified targeted, makes them promising avenue for advancing therapy. We are investigating the molecular mechanisms underlying interactions cells, particular interfere cellular processes induce apoptosis. The properties not accidental but due chemical structure reactivity. electrophilic nature these allows selectively target residues on disease-associated proteins, resulting significant therapeutic benefits minimal toxicity various diseases. opens up new perspectives development more effective precise drugs. Nevertheless, further studies essential fully understand impact our discoveries translate into clinical practice.
Language: Английский
Citations
13
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: Feb. 28, 2024
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by synovial inflammation, cartilage destruction, pannus formation and bone erosion. Various immune cells, including macrophages, are involved in RA pathogenesis. The heterogeneity plasticity of macrophages render them pivotal regulators both the induction resolution inflammatory response. Predominantly, two different phenotypes have been identified: classically activated M1 exacerbate inflammation via production cytokines, chemokines other mediators, while alternatively M2 inhibit facilitate tissue repair. An imbalance M1/M2 macrophage ratio critical during initiation progression RA. Macrophage polarization modulated various transcription factors, epigenetic elements metabolic reprogramming. Curcumin, an active component turmeric, exhibits potent immunomodulatory effects administered treatment multiple diseases, regulation subsequent cytokine as well migration mechanisms underlying therapeutic effect curcumin on In this review, we summarize which modulates function context to provide evidence for clinical application treatment.
Language: Английский
Citations
12
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3521 - 3521
Published: March 20, 2024
The phrase “Let food be thy medicine…” means that can a form of medicine and food; in other words, the diet we eat have significant impact on our health well-being. Today, this is gaining prominence as more scientific evidence suggests one’s help prevent treat disease. A rich fruits, vegetables, whole grains, lean protein reduce risk heart disease, cancer, diabetes, problems and, hand, processed foods, added sugars, saturated fats increase same diseases. Electrophilic compounds health, they are molecules covalently modify cysteine residues present thiol-rich Keap1 protein. These bind to activate NRF2, which promotes its translocation nucleus binding DNA ARE region, triggering antioxidant response protecting against oxidative stress. include polyphenols flavonoids nucleophilic but converted electrophilic quinones by metabolic enzymes such polyphenol oxidases (PPOs) sulfur foods Brassica genus (broccoli, cauliflower, cabbage, Brussel sprouts, etc.) garlic. This review summarizes current knowledge subject.
Language: Английский
Citations
8
Antioxidants, Journal Year: 2024, Volume and Issue: 13(7), P. 775 - 775
Published: June 27, 2024
Rheumatoid arthritis (RA) is a persistent autoimmune disorder that characterized by joint inflammation, discomfort, and impairment. Despite the existence of several therapeutic approaches, their effectiveness often restricted may be linked to unfavorable side effects. Consequently, there has been growing interest in investigating naturally derived compounds as plausible agents for RA disease. The objective this review summarize existing preclinical clinical evidence regarding efficacy extracted plant extracts treatment RA, focusing on anti-inflammatory, anti-oxidative, immunomodulatory properties. Some problems with using natural chemicals are uneven quality commercially available preparations poor bioavailability these compounds. Future investigations should focus improving formulations, conducting thorough trials, exploring different techniques fully utilize intrinsic potential treating RA.
Language: Английский
Citations
7
Cells, Journal Year: 2024, Volume and Issue: 13(11), P. 915 - 915
Published: May 25, 2024
Rheumatoid arthritis (RA) is a chronic inflammatory joint disease characterised by the formation of hyperplastic pannus, as well cartilage and bone damage. The pathogenesis RA complex involves broad interactions between various cells present in inflamed synovium, including fibroblast-like synoviocytes (FLSs), macrophages, T cells, among others. Under conditions, these are activated, further enhancing responses angiogenesis promoting degradation. Novel treatment methods for greatly needed, mesenchymal stromal (MSCs) have been suggested promising new regenerative immunomodulatory treatment. In this paper, we MSCs RA-FLSs, macrophages summarise studies examining use preclinical clinical studies.
Language: Английский
Citations
4
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 142, P. 112953 - 112953
Published: Sept. 2, 2024
Rheumatoid arthritis (RA) is a chronic, progressive, systemic autoimmune disease. While triptolide (TPL) and curcumin (CUR) are known to have multiple beneficial effects on RA, the combined effect of TPL CUR remains unexplored. This study aimed investigate their synergistic cell proliferation apoptosis via IL-17/NF-κB signaling pathway. The collagen-induced (CIA) rat model was established, showing severe joint synovial damage compared normal rats. Treatment with reduced severity RA in CIA alleviated serum inflammatory cytokines, such as rheumatoid factor, IL-17, TNF-α, IL-1β, IL-6. elevated levels IL-17 NF-κB rats were also inhibited, resistant aggravated by CUR. In vitro, improvement induction observed LPS-stimulated MH7A cells treated CUR, associated inhibition Taken together, may involve relieving symptoms, improving excessive proliferation, inducing resistance, inhibiting
Language: Английский
Citations
4
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 187 - 202
Published: Jan. 1, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 24, 2025
In this study an in situ forming gel for curcumin and piperine delivery is investigated as a long-lasting strategy the local treatment of inflammatory degenerative joint disease, such osteoarthritis rheumatoid arthritis. Particularly glyceryl monooleate, association with phosphatidylcholine ethanol, were employed. Different ratios between excipients tested, aim to obtain liquid form suitable subcutaneous injection, gaining semisolid consistency contact biological fluids. A formulative was conducted assess composition impact on structural properties formulations, particularly focusing injectability phase transition. Curcumin loaded, singularly or jointly, selected gels. Structural characterization, performed by X-ray scattering, revealed disordered reverse micellar phases, undergoing transition hexagonal cubic Pn3m upon hydration. vitro dialysis release demonstrated sustained both drugs over 96 h, faster case jointly loaded drugs. Mechanistic analysis water uptake studies indicated drug governed diffusion swelling/erosion lipid supramolecular structure. Furthermore, ex vivo using human skin explants suggested formulation suitability indicating that presence formed allowed double respect simple gel.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: March 7, 2025
Qingre Huoxue Decoction (QRHXD) is a traditional Chinese herbal prescription widely used in clinical practice with significant therapeutic effects on RA; however, its mechanism of action remains unclear. This study aimed to investigate the efficacy and underlying mechanisms QRHXD treating RA through research, multiomics approaches, animal experiments. We conducted 24-week which was primary treatment, collecting serum samples from patients before after treatment for integrated proteomic metabolomic analysis identify potential targets. Bioinformatics differentially expressed proteins (DEPs) differential metabolites (DMs) performed using hierarchical clustering, volcano plots, heat maps, Gene Ontology (GO), Kyoto Encyclopaedia Genes Genomes (KEGG) analysis. To validate identified targets, we constructed collagen-induced arthritis (CIA) mouse model. Clinical research has shown that can improve symptoms relevant indicators patients, including disease activity score-28 (DAS28), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), tender joint count (TJC), swollen (SJC), visual analogue scale (VAS), patient-reported outcome (PRO), health assessment questionnaire (HAQ). Proteomics metabolomics 83 DEPs 54 DMs, 46 upregulated 37 downregulated proteins, as well 11 43 metabolites. KEGG enrichment revealed are primarily associated fatty acid degradation, ferroptosis, glycerolipid metabolism, related pathways. The DMs AMPK signalling pathway, FoxO glycolysis/gluconeogenesis, MTOR so on. GO indicated were mainly apoptotic mitochondrial changes, modification processes, fatty-acyl-CoA binding, Integrated proteomics analyses increase fructose-1,6-biphosphatase 1 (FBP1) levels reduction AMP-activated kinase (AMPK) RA. inhibited FBP1 activated signalling. Animal experiments validated findings analyses, demonstrating could also delay bone destruction reduce inflammatory factor CIA mice. may RA, attenuate response, by inhibiting activating pathway.
Language: Английский
Citations
0