Low expression of miR-7-5p promotes resistance to radiotherapy in lung cancer through direct upregulation of PKP2 expression DOI Creative Commons
Fan Zhang,

Jiaxue Yang,

Xiafei Qiu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Purpose Lung cancer remains a significant global health challenge, with advanced stages often limiting surgical options and necessitating systemic therapies like radiotherapy. Resistance to radiotherapy frequently undermines treatment efficacy. This study explores the role of miR-7-5p in modulating plakophilin-2 (PKP2) expression its impact on radiosensitivity non-small cell lung (NSCLC) cells. Methods Using clonogenic assays, CCK-8 immunofluorescence staining, Western blotting, reporter gene we assessed effects overexpression inhibition A549 NSCLC Results show that enhances by increasing DNA damage (evidenced higher γ-H2AX foci) inhibiting non-homologous end joining (NHEJ) repair. Bioinformatics experimental validation identified PKP2 as direct target miR-7-5p. Overexpression mitigates radiosensitizing miR-7-5p, confirming miR-7-5p/PKP2 axis's regulating radiosensitivity. Conclusion work highlights potential targeting pathway overcome resistance NSCLC, offering promising therapeutic approach enhance outcomes.

Language: Английский

CKS1B regulates the radiosensitivity of lung cancer via activating the PI3K/AKT signaling pathway DOI
Jiangyue Lu,

Lehui Du,

Pei Zhang

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111828 - 111828

Published: April 1, 2025

Language: Английский

Citations

0
Immunogenic chemotherapy: great potential for improving response rates DOI Creative Commons
Xiaojun Huang,

Qinghuan Ren,

Leixiang Yang

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: Dec. 6, 2023

The activation of anti-tumor immunity is critical in treating cancers. Recent studies indicate that several chemotherapy agents can stimulate by inducing immunogenic cell death and durably eradicate tumors. This suggests holds great potential for improving response rates. However, practice has only had limited success long-term survival or cure cancers when used either alone combination with immunotherapy. We think this because the importance dose, schedule, tumor model dependence chemotherapy-activated under-appreciated. Here, we review immune modulation function representative propose a chemotherapy-induced long-lasting responses rely on synergetic interaction between killing cells immunity. comb through treatment schedules, identify needs dose schedule optimization therapy immunotherapy dosage responsiveness too low. further intrinsic factors affect optimal schedule. Lastly, biomarkers indicating to and/or treatments. A deep understanding how activates monitor its lead development more effective chemo-immunotherapy, thereby efficacy cancer treatment.

Language: Английский

Citations

10
Hypoxia studies in non‑small cell lung cancer: Pathogenesis and clinical implications (Review) DOI Creative Commons
Sirui Zhou, Jiazheng Sun, Weijian Zhu

et al.

Oncology Reports, Journal Year: 2024, Volume and Issue: 53(2)

Published: Dec. 30, 2024

Language: Английский

Citations

3
Unveiling Anticancer Potential of COX-2 and 5-LOX Inhibitors: Cytotoxicity, Radiosensitization Potential and Antimigratory Activity against Colorectal and Pancreatic Carcinoma DOI Creative Commons
Jelena Bošković, Vladimir Dobričić, Otilija Keta

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 826 - 826

Published: June 18, 2024

Apart from cytotoxicity, inhibitors of the COX-2 enzyme have demonstrated additional effects important for cancer treatment (such as radiosensitization tumor cells and cell antimigratory effects); however, relationship between inhibition other inflammation-related 5-LOX anticancer activity is still not well understood. In our study, cytotoxicity thirteen previously presented by group (1–13) was tested on three lines (HCT 116, HT-29 BxPC-3) one healthy line (MRC-5). Compounds 3, 5, 6 7 showed moderate but good selectivity towards lines. IC50 values were in range 22.99–51.66 µM 116 line), 8.63–41.20 (BxPC-3 line) 24.78–81.60 (HT-29 line; compound > 100 µM). comparison to tested, commercially available inhibitors, both increased. The addition compounds irradiation most significant decrease proliferation (p < 0.001). potential best dual (compounds 1, 2, 3 5) a wound-healing assay using SW620 line. 1 singled out with potent effect (relative wound closure 3.20% (24 h), 5,08% (48 h) 3.86% 7.68% 3). Considering all these results, stood optimal biological activity, inhibitory lines, lack toxic at therapeutic doses.

Language: Английский

Citations

2
Additional considerations in cancer cell radioresistance, integrin αvβ3 and thyroid hormones DOI Creative Commons
Gennadi V. Glinsky,

Aleck Hercbergs,

Shaker A. Mousa

et al.

Endocrine Research, Journal Year: 2024, Volume and Issue: 49(4), P. 251 - 254

Published: June 17, 2024

Background The existence of a functional relationship between certain thyroid hormone analogue and cancer cell radioresistance has been shown by Leith coworkers. with relevance to malignant cells' is tetraiodothyroacetic acid (tetrac). Tetrac the deaminated derivative L-thyroxine (T4), principal product gland. Preclinical studies demonstrated that tetrac chemically modified (CMT), e.g. fluorobenzyl-conjugated analogue, restores radiosensitivity in radioresistant tumor cells. Due their molecular, physico-chemical, biological properties, actions CMT analogues are believed be initiated at receptor site on plasma membrane integrin αvβ3.

Language: Английский

Citations

1
Extracellular Vesicle- and Mitochondria-Based Targeting of Non-Small Cell Lung Cancer Response to Radiation: Challenges and Perspectives DOI Open Access
Sergey Leonov, Anna Dorfman,

Elizaveta Pershikova

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(12), P. 2235 - 2235

Published: June 15, 2024

During the cell life cycle, extracellular vesicles (EVs) transport different cargos, including organelles, proteins, RNAs, DNAs, metabolites, etc., that influence proliferation and apoptosis in recipient cells. EVs from metastatic cancer cells remodel matrix of tumor microenvironment (TME), promoting invasion niche preparation. Although process is not fully understood, evidence suggests facilitate genetic material transfer between In context NSCLC, can mediate intercellular mitochondrial (Mt) transfer, delivering mitochondria organelle (MtO), DNA (mtDNA), and/or mtRNA/proteinaceous cargo signatures (MtS) through mechanisms. On other hand, certain populations hijack MtO TME mainly by using tunneling nanotubes (TNTs). This aids restoring function, benefiting benign with impaired metabolism enabling restoration their metabolic activity. However, impact transferring versus transplanting intact organelles remains uncertain subject debate. Some studies suggest EV-mediated delivery to how responds radiation. It might make more resistant or sensitive our review, we aimed point out current controversy surrounding experimental data highlight new paradigm-shifting modalities radiation therapy could potentially overcome resistance mechanisms NSCLC.

Language: Английский

Citations

1
Radiosensitization effect of quinoline-indole-schiff base derivative 10E on non-small cell lung cancer cells in vitro and in tumor xenografts DOI

Hongwei Liu,

Qianqian Wang,

Wanying Lan

et al.

Investigational New Drugs, Journal Year: 2024, Volume and Issue: 42(4), P. 405 - 417

Published: June 17, 2024

Language: Английский

Citations

1
Global profiling of transcriptome, proteome and 2-hydroxyisobutyrylome in radioresistant lung adenocarcinoma cell DOI Creative Commons
Lei Zheng,

Jiang He,

Haonan Yang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 3, 2024

Language: Английский

Citations

1
Lipidome analyses reveal radiation induced remodeling of glycerophospholipid unsaturation in lung tumor DOI Creative Commons
Jingquan He, Qingqing Yuan, Song Gao

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 24, 2024

Radiotherapy is a pivotal treatment for lung cancer, significantly impacting tumor control and patient quality of life. Despite its benefits, the molecular mechanisms underlying radiotherapy-induced biological alterations in cancer cells remain inadequately understood. In this study, we employed mass spectrometry-based lipidomics approach to investigate lipid profile changes mouse model post-radiation. Lewis carcinoma (LLC) were injected into C57BL/6J mice, followed by radiation with varying split doses. Our results showed an increase sterol lipids decrease glycerolipids, specifically triacylglycerides, indicating disrupted storage. Additionally, observed significant glycerophospholipid unsaturation, suggesting remodeling membrane properties that may influence cell survival. Linear regression analysis demonstrated negative correlation between unsaturation index weight, potential role radiation-induced death. These findings provide new insights metabolic pathways affected radiotherapy could inform development improved therapeutic strategies treatment.

Language: Английский

Citations

1
Mathematical Modeling Support for Lung Cancer Therapy—A Short Review DOI Open Access
Jarosław Śmieja

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14516 - 14516

Published: Sept. 25, 2023

The paper presents a review of models that can be used to describe dynamics lung cancer growth and its response treatment at both cell population intracellular processes levels. To address the latter, signaling pathways associated with cellular responses are overviewed. First, options for discussed, main regulatory networks briefly reviewed. Then, approaches model specific therapies discussed. Following that, crucial in presented. is concluded discussion applicability presented context cancer.

Language: Английский

Citations

3