Investigating Immunomodulatory Biomaterials for Preventing the Foreign Body Response

Bioengineering, Journal Year: 2023, Volume and Issue: 10(12), P. 1411 - 1411

Published: Dec. 11, 2023

Implantable biomaterials represent the forefront of regenerative medicine, providing platforms and vessels for delivering a creative range therapeutic benefits in diverse disease contexts. However, chronic damage resulting from implant rejection tends to outweigh intended healing benefits, presenting considerable challenge when implementing treatment-based biomaterials. In response rejection, proinflammatory macrophages activated fibroblasts contribute synergistically destructive process uncontrolled inflammation excessive fibrosis. Understanding complex biomaterial-host cell interactions that occur within tissue microenvironment is crucial development promote integration minimize foreign body response. Recent modifications specific material properties enhance immunomodulatory capabilities biomaterial actively aid taming immune by tuning with surrounding either directly or indirectly. By incorporating amplify anti-inflammatory pro-regenerative mechanisms, can be optimized maximize their harmony host system.

Language: Английский

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A new ginger extract characterization: Immunomodulatory, antioxidant effects and differential gene expression

Food Bioscience, Journal Year: 2023, Volume and Issue: 53, P. 102746 - 102746

Published: May 20, 2023

Ginger (Zingiber officinale) is commonly consumed as spice or herbal medicine with anti-inflammatory, antioxidant, and anticancer properties. It rich of many bioactive constituents, mainly gingerols shogaols. Although the constituents have been identified, molecular mechanisms ginger action are still limited, related signalling pathways not completely defined. Here, we used a simple ethanol/freeze-drying method to obtain new extract (GE), which was chemically characterised by Folin-Ciocalteu, antioxidant ORAC HPLC assays. At cellular level, anti-inflammatory/antioxidant properties GE, in addition commercial [6]-gingerol, were evaluated using RAW264.7 murine macrophages. Cell viability tests verified non-toxic doses GE glutathione assay confirmed property GE. By quantitative PCR, analysed differential expression various genes LPS-treated cells, after GE/[6]-gingerol pre-treatments. The belonged different categories: immune signalling, pro-/anti-inflammatory cytokines, pro-/anti-antioxidant enzymes, hallmarks macrophage polarization endoplasmic reticulum stress response. Results showed that pre-treatment two reduced LPS-induced TLR4, MyD88, Rel-A, IL-1α, IL-6, TNF-α, IL-10, iNOS TRIB3 varying degrees, whereas increased Jun, Light/Tnfsf14, HO-1 Arg-1 gene expression. No effect found on MIF cells These results also suggested promotes specific markers LPS-stimulated trend activate an anti-inflammatory M2 phenotype. Further analyses will broaden understanding role individual components inflammation/immunomodulation.

Language: Английский

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Exploring the role of CITED transcriptional regulators in the control of macrophage polarization

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 5, 2024

Macrophages are tissue resident innate phagocytic cells that take on contrasting phenotypes, or polarization states, in response to the changing combination of microbial and cytokine signals at sites infection. During opening stages an infection, macrophages adopt proinflammatory, highly antimicrobial M1 state, later shifting anti-inflammatory, pro-tissue repair M2 state as infection resolves. The changes gene expression underlying these transitions primarily governed by nuclear factor kappaB (NF-κB), Janus kinase (JAK)/signal transducer activation transcription (STAT), hypoxia-inducible 1 (HIF1) factors, activity which must be carefully controlled ensure effective yet spatially temporally restricted inflammatory response. While much this control is provided pathway-specific feedback loops, recent work has shown transcriptional co-regulators CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxy-terminal domain (CITED) family serve common controllers for pathways. In review, we describe how CITED proteins regulate polarization-associated controlling ability factors form chromatin complexes histone acetyltransferase, CBP/p300. We will also cover differences interactions between CITED1 2 CBP/p300 drive their effects pro-inflammatory expression.

Language: Английский

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Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 1, 2024

Introduction Recently, the use of botanicals as an alternative to coccidiostats has been appealing approach for controlling coccidiosis. Therefore, this study was conducted evaluate potential role aqueous methanolic extract (200 mg/kg) Krameria lappacea (roots) (KLRE) against infection induced by Eimeria papillata . Methods A total 25 male C57BL/6 mice were divided into five groups (I, II, III, IV, and V). On 1 st day experiment, all except I (control) II (non-infected-treated group with KLRE), inoculated orally 10 3 sporulated E. oocysts. infection, IV treated KLRE. Group V served infected-treated amprolium (coccidiostat). Results Treatment coccidiostat continued consecutive days. While not reaching efficacy level reference drug (amprolium), KLRE exhibited notable anticoccidial activity assessed key criteria, including oocyst suppression rate, parasitic stages, maintenance nutrient homeostasis. The presence phenolic flavonoid compounds in is thought be responsible its positive effects. increased oxidative damage jejunum. treatment significantly catalase superoxide dismutase. contrary, decreased malondialdehyde nitric oxide. Moreover, macrophage infiltration jejunal tissue, well extent CD4 T cells NFkB. caused a state systemic inflammatory response revealed upregulation inducible oxide synthase ( iNOs )-mRNA. Upon KLRE, reduced from 3.63 1.46 fold. able downregulate expression pro-inflammatory cytokines interferon-γ, nuclear factor kappa B, interleukin-10 -mRNA 1.63, 1.64, 1.38 fold, respectively. showed significant reduction IL-10 protein 104.27 ± 8.41 pg/ml 62.18 pg/ml. Conclusion Collectively, K. promising herbal medicine that could ameliorate stress inflammation jejunum, mice.

Language: Английский

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SARS-CoV-2 envelope protein regulates innate immune tolerance

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109975 - 109975

Published: May 15, 2024

Language: Английский

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