Milk composition and health status of quarters adjacent to an on-farm protocol-treated clinical-mastitis gland DOI
Marcel Gomes Paixão, Renata Golin Bueno Costa, Guilherme Nunes de Souza

et al.

Animal Production Science, Journal Year: 2024, Volume and Issue: 64(4)

Published: Feb. 16, 2024

Context The hypothesis of interdependency among cows’ mammary glands has been shown, and quarters adjacent to with clinical mastitis may have their composition health status impaired. Aims main focus this study was compare foremilk (fat, total protein, lactose, solids non-fat (SNF), chloride) (somatic cell count (SCC), differential leukocytes) healthy a gland previously treated (on average, 24 days after treatment, varying from 20 39 days) for samples cows. Our previous compared these traits (foremilk status) between mastitis-infected quarter cows (no mastitis) immediately the identification on case quarters. Methods designed as prospective case–control enrolled (cases controls) were matched by in milk parity. Case (n = 50) defined that had single infected mild or moderate mastitis, which according an on-farm protocol 1 day identification. Control not suffered current lactation. Foremilk each control collected so assess concentrations fat, SCC, SNF, conduct microbiological analyses, determine concentration chloride leukocyte counts. Multivariate linear regression logistic analyses performed possible associations milk-composition health-status types (case cows). Key results On treatment began, some (lymphocyte macrophage counts) returned similar levels quarters, while neutrophils remained greater No effect position identified. Conclusions suggested at least (average episode neighbouring gland, still recovering episode, we conclude is physiological systemic two-way immune response route, particular component different behaviour mastitis. Implications suggest far more costly producers industry than reported. Bovine disease causes most economical losses dairy farmers, including veterinary expenses, disposal, loss production reduction animal pregnancies. affected only one compromised, because quality entire udder also affected. A period enough animals fully recover inflammation. Mastitis

Language: Английский

Update on the roles and applications of extracellular vesicles in depression DOI
Jing Wu, Jian Lü,

Muyi Pan

et al.

World Journal of Psychiatry, Journal Year: 2025, Volume and Issue: 15(3)

Published: Feb. 26, 2025

Depression is a prevalent mental disorder that affects numerous individuals, manifesting as persistent anhedonia, sadness, and hopelessness. Despite extensive research, the exact causes optimal treatment approaches for depression remain unclear. Extracellular vesicles (EVs), which carry biological molecules such proteins, lipids, nucleic acids, metabolites, have emerged crucial players in both pathological physiological processes. EVs derived from various sources exert distinct effects on depression. Specifically, released by neurons, astrocytes, microglia, oligodendrocytes, immune cells, stem even bacteria contribute to pathogenesis of Moreover, there growing interest potential diagnostic therapeutic tools This review provides comprehensive overview recent research different sources, their roles depression, clinical applications.

Language: Английский

Citations

0
The Oral–Gut Microbiome–Brain Axis in Cognition DOI Creative Commons

Noorul Ain Adil,

Christabel Omo-Erigbe,

Hariom Yadav

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 814 - 814

Published: April 3, 2025

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline and neuronal loss, affecting millions worldwide. Emerging evidence highlights the oral microbiome—a complex ecosystem of bacteria, fungi, viruses, protozoa as significant factor in health. Dysbiosis microbiome contributes to systemic inflammation, disrupts blood–brain barrier, promotes neuroinflammation, processes increasingly implicated pathogenesis AD. This review examines mechanisms linking dysbiosis through oral–brain oral–gut–brain axis. These interconnected pathways enable bidirectional communication between cavity, gut, brain via neural, immune, endocrine signaling. Oral pathogens, such Porphyromonas gingivalis, along with virulence factors, including lipopolysaccharides (LPS) gingipains, contribute while metabolic byproducts, short-chain fatty acids (SCFAs) peptidoglycans, further exacerbate immune activation. Additionally, this explores influence external diet, pH balance, medication use, smoking, alcohol consumption, hygiene, on microbial diversity stability, highlighting their role shaping outcomes. The dynamic interplay gut microbiomes reinforces importance homeostasis preserving neurological interventions, probiotics, prebiotics, dietary modifications, offer promising strategies support function reduce risk diseases, AD, maintaining diverse microbiome. Future longitudinal research needed identify long-term impact cognition.

Language: Английский

Citations

0
Exploring Gut Microbiota in Systemic Lupus Erythematosus: Insights and Biomarker Discovery Potential DOI

Lingyue Jin,

Jie Xiao, Yuhong Luo

et al.

Clinical Reviews in Allergy & Immunology, Journal Year: 2025, Volume and Issue: 68(1)

Published: April 11, 2025

Language: Английский

Citations

0
Segatella copri Outer-Membrane Vesicles Are Internalized by Human Macrophages and Promote a Pro-Inflammatory Profile DOI Open Access

Alison Sepúlveda-Pontigo,

Karissa Chávez-Villacreses,

Cristóbal Madrid-Muñoz

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3630 - 3630

Published: April 11, 2025

Increased abundance of Segatella copri (S. copri) within the gut microbiota is associated with systemic inflammatory diseases, including rheumatoid arthritis. Although outer-membrane vesicles (OMVs) Gram-negative bacteria are important players in microbiota–host communication, effect S. copri-derived OMVs on immune cells unknown. Macrophages engulf and eliminate foreign material conditioned by environmental signals to promote either homeostasis or inflammation. Thus, we aimed explore impact copri-OMVs human macrophages vitro, employing THP-1 monocyte-derived macrophage models. The uptake DiO-labeled into was monitored confocal microscopy flow cytometry. Furthermore, phenotype cytokine secretion naïve (M0), pro-inflammatory (M1), anti-inflammatory (M2) analyzed cytometry ELISA, respectively. We show that enter through macropinocytosis clathrin-dependent mechanisms. copri-OMVs, but not parental bacterium, induced a dose-dependent increase expression M1-related surface markers M0 M2 activated large amounts cytokines M1 macrophages. These results highlight an role promoting responses, which might contribute diseases.

Language: Английский

Citations

0
Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression DOI Creative Commons
Pengcheng Ma, Ruoyi Wang,

Huizhi Chen

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 11, 2025

Purpose To study whether fecal microbiota transplantation (FMT) can alleviate lipopolysaccharide (LPS)-induced osteoporosis (OP) by regulating the composition and abundance of gut expression level long non-coding RNA (lncRNA) TUG1 . Methods Twenty C57BL/6 mice were selected. Two randomly designated as donors, while remaining divided into control group, LPS + FMT group. Each group consisted 6 mice. The in groups intraperitoneally injected with to establish OP model, treated donor feces gavage. Micro-CT was used scan femur specimens mice, bone structural parameters compared verify effectiveness model. HE staining compare microstructure femurs 3 groups. 16S rRNA gene sequencing analyze Immunofluorescence levels Runt-related transcription factor 2 (RUNX2) Real-time quantitative reverse PCR (qRT-PCR) lncRNA intestines serum Results showed that had more loss. mineral density, trabecular number, thickness higher, separation smaller. models validated effectively. trabeculae thinner sparse, dense clear. 16s Bacteroides Lactobacillus LPS+FMT significantly higher than RUNX2 similar, both qRT-PCR results mRNA similar Conclusion enhance osteoblast improve structure modulating (such increasing populations) promoting , thereby alleviating LPS-induced OP.

Language: Английский

Citations

0
NDM-5-carried outer membrane vesicles impair the efficacy of antibiotics against bacterial infections DOI Creative Commons
Lin Li, Yanfang Zhang,

Lihui Weng

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

ABSTRACT The intensifying use of antimicrobials in the rapidly growing livestock industry has heightened concerns over proliferation antibiotic resistance, particularly among Enterobacteriaceae producing β-lactamase. Elucidating role β-lactamase could unlock novel strategies to combat drug-resistant and poultry farming. Outer membrane vesicles (OMVs) produced by gram-negative bacteria have ability encapsulate transport components derived from their parental bacteria. This raises intriguing possibility that OMVs harbor drug-resistance enzymes, thereby conferring protection susceptible against antibiotics. Here, we successfully extracted New Delhi metallo-β-lactamase-5 (NDM-5)-expressing Escherichia coli confirmed these indeed carry NDM-5 protein. Furthermore, bacterial assays showed cause sensitive treated with meropenem restore growth activity, degradation was verified using high-performance liquid chromatography. Lastly, survival rate intervention group significantly lower than drug-treated a Galleria mellonella larvae infection model, validating protective effect on increasing tolerance meropenem. These findings illustrate can serve as vehicles for resistance-related factors, promoting

Language: Английский

Citations

0
Engineered Bacterial Outer Membrane Vesicles Co-Delivering Angio-3 and Doxorubicin to Enhance Tumor Therapy DOI
Shuai Zhao, Wenxuan Xu,

Wanting Zeng

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114707 - 114707

Published: April 18, 2025

Language: Английский

Citations

0
Bacteroidetes promotes esophageal squamous carcinoma invasion and metastasis through LPS-mediated TLR4/Myd88/NF-κB pathway and inflammatory changes DOI Creative Commons
Zhongbing Wu, Jianxin Guo, Zhenhuan Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 4, 2024

Abstract Gut microbiota plays a crucial role in gastrointestinal tumors. Additionally, gut microbes influence the progression of esophageal cancer. However, major bacterial genera that affect invasion and metastasis cancer remain unknown, underlying mechanisms unclear. Here, we investigated flora metabolites patients with squamous cell carcinoma found abundant Bacteroides increased secretion entry surface antigen lipopolysaccharide (LPS) into blood, causing inflammatory changes body. We confirmed these results mouse model 4NQO-induced situ further identified epithelial–mesenchymal transition (EMT) occurrence TLR4/Myd88/NF-κB pathway activation vitro experiments revealed LPS from fragile promoted proliferation, migration, invasion, induced EMT by activating pathway. These reveal are closely associated through higher response level signaling both common to inflammation tumors LPS, providing new biological target for prevention or treatment.

Language: Английский

Citations

3
Dynamic shielding of bacterial outer membrane vesicles for safe and efficient chemo-immunotherapy against tumors DOI

Shuping Li,

Xin Li, Jiaqi Meng

et al.

Nano Research, Journal Year: 2023, Volume and Issue: 17(2), P. 836 - 847

Published: Nov. 17, 2023

Language: Английский

Citations

7
The role of the microbiome in head and neck squamous cell cancers DOI
Taha Koray Şahin, Meli̇ha Çağla Sönmezer

European Archives of Oto-Rhino-Laryngology, Journal Year: 2024, Volume and Issue: 282(2), P. 623 - 637

Published: Sept. 21, 2024

Language: Английский

Citations

2