Functional properties of chia (Salvia hispanica L.) flour, its hydrolyzed protein and phenolic extract on microbiota and gut health in vivo DOI Open Access
Marcella Duarte Villas Mishima

Published: Dec. 19, 2023

Chia has a composition with potential to promote changes in the intestinal tissue that favor its functionality, morphology, and microbiota. Objective: evaluate functional properties of chia flour, hydrolyzed protein phenolics on microbiota gut health vivo. Methodology: four experimental trials were conducted. First experiment: 32 male Wistar rats received following diets (5 weeks): standard (SD), SD+chia, high-fat diet (HFD), or HFD+chia. Second 64 female fed either SD HFD (7 weeks). Then, underwent ovariectomy (OVX) while surgery without removal ovary (SHAM). After 3-week recovery period, divided into eight groups (8 SD, HFD, HFD+chia, for both OVX SHAM groups. Intestinal microbiota, short-chain fatty acids (SCFA), IgA production, pH, histomorphometry, brush border membrane functionality analyzed. systematic review was conducted according PRISMA guidelines, answer question: “How does food derived bioactive peptides can impact inflammatory mediators vivo?”. The third fourth experiments ovo. Third 45 fertile eggs (Gallus gallus) five receiving different treatments: not injected; 18MΩH20; 10mg/mL (1%); protein+106 CFU Lacticaseibacillus paracasei 106 paracasei. Fourth 27 three groups: 18MΩH 20; (1%) phenolic extract. Upon hatching, animals euthanized analysis composition, gene expression related inflammation, barrier proteins. Results: Paper 1: flour consumption increased SCFA production improved circular muscle layer. Diversity abundance bacteria affected, but richness increased. Proteins associated downregulated. 2: In rats, intake acetic butyric decreased cecum content layers crypt thickness, diversity, Ap Si. 3: group propionic acid pH cecal content, reducing concentration HFD+chia group. Nevertheless, it microbial diversity. SD+chia Si Sglt1 Pept1. 4: highlighted positive effects inflammation health. 5: downregulated Tnf-α, Ocln, Muc2, Ap, reduced Bifidobacterium, Lactobacillus morphology. 6: extract Tnf-α expression, E. coli populations Paneth cell diameter, depth crypt, maintained villus height. Conclusion: be considered biological improve demonstrated fractions seed. Key words: Microbiota; Morphology; Short Chain Fatty Acids; Functionality; Intra-Amniotic Administration; paracasei; Brush Border Membrane; Ovariectomy.

Language: Английский

Biochemical Conversion of Passion Fruit Waste Into Highly Bioaccessible, Stable, and Selectively Functional Products DOI Creative Commons

Huyen Ngoc Nguyen,

Anh T. Huynh,

Mariana Bárcenas

et al.

Waste and Biomass Valorization, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Language: Английский

Citations

3
Fermentation-Mediated Enhancement of Safety, Thermal Stability, Bioaccessibility, and Health-Promoting Potential of Watermelon Peel Polyphenol Extract DOI

Lam Vi Chau,

Huyen Ngoc Nguyen,

Minh Thang Le

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Language: Английский

Citations

1
A novel black poplar propolis extract with promising health-promoting properties: focus on its chemical composition, antioxidant, anti-inflammatory, and anti-genotoxic activities DOI Creative Commons
Mattia Acito, Ina Varfaj,

Virginia Brighenti

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(9), P. 4983 - 4999

Published: Jan. 1, 2024

Propolis is a resinous mixture produced by honeybees which has been used since ancient times for its useful properties. However, chemical composition and bioactivity may vary, depending on the geographical area of origin type tree bees use collecting pollen. In this context, research aimed to investigate total phenolic content (using Folin-Ciocalteu assay) antioxidant capacity FRAP, DPPH, ABTS assays) three black poplar (Populus nigra L.) propolis (BPP) solutions (S1, S2, S3), as well (HPLC-ESI-MSn) biological activities (effect cell viability, genotoxic/antigenotoxic properties, anti-inflammatory activity, effect ROS production) one showed highest activity (S1). The hydroalcoholic BPP solution S1 was prototype an innovative, research-type product Italian nutraceutical manufacturer. contrast, S2 S3 were conventional products purchased from local pharmacy stores. For extracts, 50 compounds, encompassing acids flavonoids, identified. summary, results interesting profile remarkable antioxidant, antigenotoxic, ROS-modulating innovative extract S1, paving way future research. vivo investigations will be possible line take, help corroborate hypothesis potential health benefits product, even stimulate further ameliorations new prototype.

Language: Английский

Citations

7
A promising fusion: Traditional Chinese medicine and probiotics in the quest to overcome osteoporosis DOI Open Access
Kunyang Fan, Xin Hua, Shuwan Wang

et al.

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(5)

Published: March 6, 2025

Botanical drugs and probiotic supplements present safer alternative options for the prevention treatment of osteoporosis (OP). However, pathological disorders gut microbiota specific properties probiotics traditional Chinese medicine (TCM) significantly limit their therapeutic efficacy. Given favorable synergistic complementary effects between herbal medicines, a creative combination these approaches may address issue current limited A comprehensive analysis is necessary to provide detailed review potential combination, mechanisms behind synergy, scientific applications, future developments. There exists complex relationship OP, underlying regulatory are multidimensional, involving production pro-inflammatory metabolites, immune system disruption, impairment intestinal mucosal barrier. Furthermore, we analyzed connections probiotics, TCM, combined applications. We highlighted principle gain substantial organic which facilitates release active substances in increases bioavailability enhances delivery efficiency, exerts effects. The use TCM offers safe effective strategy managing OP presents an innovative promising direction development modern phytomedicine.

Language: Английский

Citations

0
Obtainment of Flavonoid-Enriched Fractions from Maqui (Aristotelia chilensis) and Murta (Ugni molinae) Extracts via Preparative HPLC and Evaluation of Their Anti-Inflammatory Effects in Cell-Based Assays DOI Creative Commons

Amador Alburquenque,

Claudia Busch,

Gabriela Gómez-Lillo

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 600 - 600

Published: May 16, 2025

Polyphenols exert anti-inflammatory and antioxidant effects by modulating cell signalling pathways transcription factors involved in inflammatory bowel disease (IBD). However, their stability during digestion can be compromised. Polymer coatings like chitosan (-C) help preserve stability. Maqui (Aristotelia chilensis) murta (Ugni molinae) are rich compounds. This work aims to obtain extracts (E) blends (B) enriched delphinidin quercetin glucosides from maqui (Ma) (Mu) crude using preparative chromatography methodology (Prep-HPLC) evaluate effectiveness through vitro cellular assays. HPLC-DAD analysis revealed a marked increase phenolic compound concentration the BEMaMu BCMaMu extract blends. Total glycosides (TQG) increased 11-fold, total anthocyanins approximately 8-fold compared fruit blend (BMaMu). exhibited significantly higher ORAC value estimated additive mixture, suggesting synergistic effect. No cytotoxicity was observed for BEMaMu, BCMaMu, chitosan-coated versions (BEMaMu-C BCMaMu-C) Caco-2 HT29-MTX-E12 cells at concentrations of 0.1–50 mg/mL. Notably, only inhibited NF-κB expression activated Nrf2 TNF-α-challenged 0.1 0.5

Language: Английский

Citations

0
Deciphering the gut microbiome’s metabolic code: pathways to bone health and novel therapeutic avenues DOI Creative Commons

Daniel Hwang,

Esther G Chong, Yanxiu Li

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: May 22, 2025

The gut microbiome plays an important role in the protection against various systemic diseases. Its metabolic products profoundly influence a wide range of pathophysiological events, including regulation bone health. This review discusses recently established connections between and metabolism, focusing on impact microbiome-derived metabolites such as SCFAs, Bile Acids, tryptophan to control remodeling immunoreactions. Recent advances metagenomics profiling have unveiled new exciting therapeutic opportunities, ranging from use probiotics, prebiotics, engineered microbes, fecal microbiota transplantation. Understanding interplay among diet, microbiota, health provides avenues for tailored interventions aimed at reducing disease risk osteoporosis other related disorders. By drawing knowledge microbiology, biology, this highlights potential microbiome-targeted therapies transform skeletal management

Language: Английский

Citations

0
The Intriguing Connection Between the Gut and Lung Microbiomes DOI Creative Commons
Magdalena Druszczyńska, Beata Sadowska,

Jakub Kulesza

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(11), P. 1005 - 1005

Published: Nov. 15, 2024

Recent advances in microbiome research have uncovered a dynamic and complex connection between the gut lungs, known as gut-lung axis. This bidirectional communication network plays critical role modulating immune responses maintaining respiratory health. Mediated by interactions, metabolic byproducts, microbial communities both organs, this axis demonstrates how gut-derived signals, such metabolites modulators, can reach lung tissue via systemic circulation, influencing function disease susceptibility. To explore implications of connection, we conducted systematic review studies published 2001 2024 (with much nearly 60% covering period 2020-2024), using keywords "gut-lung axis", "microbiome", "respiratory disease", "immune signaling". Studies were selected based on their relevance to mechanisms, impact dysbiosis, microbiota diseases. provides comprehensive overview axis, emphasizing its importance regulating inflammatory linked Understanding intricate pathway opens new avenues for microbiota-targeted therapeutic strategies, which could offer promising interventions diseases like asthma, chronic obstructive pulmonary disease, even infections. The insights gained through underscore potential novel target preventative approaches medicine, with enhancing

Language: Английский

Citations

3
Polyphenols Influence the Development of Endometrial Cancer by Modulating the Gut Microbiota DOI Open Access
Ewa Baranowska‐Wójcik,

Anna Winiarska‐Mieczan,

Piotr Olcha

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(5), P. 681 - 681

Published: Feb. 28, 2024

Dysbiosis of the microbiota in gastrointestinal tract can induce development gynaecological tumours, particularly postmenopausal women, by causing DNA damage and alterations metabolite metabolism. also complicates cancer treatment influencing body’s immune response disrupting sensitivity to chemotherapy drugs. Therefore, it is crucial maintain homeostasis gut through effective use food components that affect its structure. Recent studies have shown polyphenols, which are likely be most important secondary metabolites produced plants, exhibit prebiotic properties. They structure synthesis metabolites. In this review, we summarise current state knowledge, focusing on impact polyphenols endometrial cancer, emphasising polyphenol consumption leads beneficial modifications microbiota.

Language: Английский

Citations

2
The Importance of Argan Oil in Medicine and Cosmetology DOI Open Access
Agata Serrafi, Fatima Chegdani,

Faïza Bennis

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(20), P. 3573 - 3573

Published: Oct. 21, 2024

Argan oil, rich in unsaturated fatty acids and polyphenols, exerts beneficial effects on both the intestinal skin microbiotas. In gut, it promotes growth of bacteria, such as

Language: Английский

Citations

1
miR-595/Cldnd1 axis: a potential risk factor for bone loss in postmenopausal women with hip osteoporotic fracture DOI Creative Commons

Jingyue Sun,

Peixin Liu, Xiao Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Abstract Background Recently researches have reported that miRNA and its target genes are associated with osteoporosis. MiRNAs/mRNA axis might be an potential diagnostic marker for Purposes The aim of this study is to explore the mRNA markers by bioinformatics method clinical analysis. Patients Methods expression profiles were obtained from GSE74209, GSE64433 GSE115773 in Gene Omnibus (GEO). GSE100609. Wayne intersection used different expressed miRNAs (DE-miRs). Select highest Fold Change subsequent research. Screening using TargetScan miRDB tools. GO KEGG analyses (TGs) function performed. Validate selected TGs We collected female patients femural intertrochanteric fractures July 1, 2023 October 31, 2023. Patient's bone marrow data collected. MiRNA differentially verified through RT-qPCR. All subjected Shapiro-Wilk test. Using Pearson or Spearman test detect correlation between various indicators, then incorporating indicators related density into multiple linear regression equations. Partial analysis was analyze final density. Results A total 140 DE-miRs identified high low women. Set fold change “>1” ultimately include 5 miRNAs. miR-595 (highest |log2 FC|) as subject 3542 targeted mRNAs predicted 362 miRDB. 337 intersected, which mainly enriched nucleus. Only Cldnd1 GSE100609 dataset. found highly mass, while gene downregulated. There a strong collinearity Cldnd1. Further showed Conclusions These suggest downstream factor miR-595. miR-595/Cldnd1 independent risk decreased mass.

Language: Английский

Citations

0