Digital Chinese Medicine,
Journal Year:
2023,
Volume and Issue:
6(3), P. 295 - 306
Published: Sept. 1, 2023
The
present
study
was
aimed
to
investigate
the
neuroprotective
effect
of
Croton
hirtus
(CH)
extract
against
streptozotocin
(STZ)
in
rats.
(i)
sub-chronic
toxicity
consisted
24
adult
rats
either
sex
weighing
from
160
200
g
were
divided
into
four
groups
with
six
each
group.
Rats
group
1
received
Dimethyl
sulfoxide
(DMSO)
mixed
saline;
2,
3,
and
4
100,
200,
400
mg/kg
methanolic
CH
(MECH)
orally
by
gavage
administration
for
28
d,
respectively.
functional
observation
battery
locomotor
activity
graded
as
part
their
neurobehavioral
brain
regions,
including
cortex
hippocampus,
analyzed
neuropathological
abnormalities.
(ii)
main
research
30
male
Wistar
g,
which
five
group,
control
(I),
STZ
(II),
Donepezil
(III),
MECH
(100
mg/kg,
IV),
(200
V)
groups.
I
citrate
buffer
DMSO
saline
14
d.
II
via
intracerebroventricular
injection
(3
bilateral
ICV-STZ)
on
days
3
followed
III,
IV,
V
[3
mg/(kg·d),
p.o.]
[100
tested
learning
memory
parameters
such
Morris
water
maze
(MWM)
passive
avoidance
test
(PAT).
They
sacrificed
after
completing
behavioural
experiments;
brains
harvested
estimate
acetylcholinesterase
(AChE),
lipid
peroxidation
(LPO),
glutathione
(GSH),
superoxide
dismutase
(SOD)
using
UV-Visible
Spectrophotometer;
caspase-3
evaluated
total
fluorescence
emission
spectra;
amyloid
β
(Aβ)
levels
detected
enzyme-linked
immuosorbent
assay
(ELISA);
histopathological
examination
conducted
CA1
region
hippocampus.
results
revealed
that
open
field
line
crossing,
rearing,
entering
middle
square,
defecating,
or
urinating
did
not
differ
significantly
(P
>
0.05).
show
any
lesions
neuronal
cells
inflammation
both
regions
compared
findings
demonstrated
STZ-treated
showed
a
significant
increase
impairment
<
0.001),
AChE,
caspase-3,
Aβ
(1-40
1-42),
LPO
increased
decrease
found
SOD
0.001)
GSH
0.01).
Moreover,
damage
In
contrast,
STZ-induced
biochemical
impairments
considerably
decreased
treatment
dose-dependently.
prevented
deficit
induced
due
antioxidant
action.
Restoration
cholinergic
functioning
may
be
cause
improvement.
Therefore,
able
treat
cognitive
disorders
like
Alzheimer's
disease
(AD).
Frontiers in Cardiovascular Medicine,
Journal Year:
2024,
Volume and Issue:
11
Published: Oct. 1, 2024
Non-alcoholic
fatty
liver
disease
(NAFLD),
now
known
as
metabolic-associated
steatotic
(MASLD),
is
the
most
common
worldwide,
with
a
prevalence
of
38%.
In
these
patients,
cardiovascular
(CVD)
number
one
cause
mortality
rather
than
disease.
Liver
abnormalities
per
se
due
to
MASLD
contribute
risk
factors
such
dyslipidemia
and
obesity
increase
CVD
incidents.
this
review
we
discuss
hepatic
pathophysiological
changes
leading
risks,
including
sinusoidal
endothelial
cells,
insulin
resistance,
oxidative
stress
focus
on
glutathione
metabolism
function.
an
era
where
there
increasingly
robust
recognition
what
causes
CVD,
included
by
American
Heart
Association
in
recently
developed
PREVENT
equation,
inclusion
may
open
doors
how
approach
treatment
for
patients
who
are
at
CVD.
Journal of Pharmaceutical and Biomedical Analysis,
Journal Year:
2024,
Volume and Issue:
242, P. 116010 - 116010
Published: Feb. 10, 2024
In
this
study,
an
alternative
method
to
compendial
analytical
procedures
with
enhanced
detection
and
separation
capabilities
was
validated
for
the
quality
assessment
of
glutathione
(GSH)
drug
substance.
The
related
impurities
A,
B,
C,
D
present
in
GSH
substance
were
characterized
using
a
one-dimension
proton
nuclear
magnetic
resonance
(1D
1H
NMR)
on
600
MHz
spectrometer
equipped
liquid
nitrogen
cryoprobe.
Two
sample
preparations
at
different
pH
optimized
ensure
unambiguous
identification
samples.
Specifically,
A
C
can
be
tested
3.0,
while
7.4
is
more
suitable
testing
B
D.
quantitative
NMR
(qNMR)
following
International
Council
Harmonisation
(ICH)
guidelines.
limit
(LOD)
less
than
0.1%
wt
individual
impurity,
quantitation
(LOQ)
ranged
from
0.14
0.24%
wt,
about
14
minutes
experimental
time
per
spectrum.
Following
validation,
qNMR
applied
assess
commercial
bulk
samples,
in-house
compounded
product,
dietary
supplement
product.
also
monitor
degradation
(hydrolysis
oxidation)
over
provide
information
stability.
results
suggest
that
serve
as
highly
specific
efficient
orthogonal
tool
assessing
pharmaceuticals,
providing
both
qualitative
its
A-D.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 20, 2024
AbstractIntroduction:
Balloon
flower
root-derived
exosome-like
nanoparticles
(BDEs)
have
recently
been
proposed
as
physiologically
active
molecules
with
no
cytotoxicity.
However,
the
therapeutic
effects
of
drug-induced
hepatotoxicity
BDEs
not
elucidated.
contain
a
large
amount
platycodin
D,
which
is
widely
known
to
be
effective
in
regulating
inflammation
and
ameliorating
systemic
toxicity.
Thus,
main
activity
attributed
inhibiting
inflammatory
response
alleviating
Objective:
Considering
potential
BDEs,
achieve
synergistic
improve
outcomes,
we
constructed
hybrid
soy
lecithin-based
liposome
loaded
silymarin
(SM).
Since
liposomes
can
provide
higher
thermal
stability
greater
structural
integrity,
these
might
more
resistant
clearance
enzymatic
degradation
drug
molecules.
Methods:
Hybrid
liposome-loaded
SM
(BDEs@lipo-SM)
were
fabricated
by
thin-film
hydration
extrusion.
BDEs@lipo-SM
characterized
using
dynamic
light
scattering
high-performance
liquid
chromatography.
After
confirmation
physical
properties
BDEs@lipo-SM,
various
evaluated.
Results:
BDEs@lipo-SM
internalized
hepatocytes
immune
cells
significantly
decreased
mRNA
expression
apoptosis
inflammation-relevant
cytokines
hepatocyte
MAPK
pathway.
induced
an
increase
glutathione
levels
inhibited
APAP-induced
hepatotoxicity.
Conclusion:
benefits
for
acute
liver
injury
alleviate
cell
death
or
accelerate
recovery
model,
highlights
thathybrid
represent
attractive
delivery
vehicle
applications.
Ural Medical Journal,
Journal Year:
2024,
Volume and Issue:
23(5), P. 89 - 103
Published: Oct. 18, 2024
Purpose
—
to
investigate
the
dynamics
of
content
antioxidant
enzymes
superoxide
dismutase
1
(SOD1),
glutathione
peroxidase
(GPO1),
reductase
(GR)
and
lipid
peroxidation
products
diene
conjugates
(DC),
malondialdehyde
(MDA)
in
spleen
liver
during
latent
period
growth
metastasis
experimental
tumor.
Materials
methods
.
Using
28
white
male
rats,
a
model
hematogenous
was
created
by
transplanting
sarcoma
45
cells
(S45)
into
spleen,
previously
lead
out
under
skin
3
weeks
before.
Previously,
determined
that
tumor
visualized
at
5
weeks,
metastases
7
after
transplantation
S45.
Levels
SOD1,
GPO1,
GR
MDA
were
using
ELISA
DC
biochemical
method
homogenates
(1–2
post-transplantation).
Results
Significant
changes
(1.5–5.2
times,
р
<
0.050–0.001)
studied
factors
levels
observed
compared
intact
rats
with
out.
Activation
system
noted
(tumor-carrying
organ)
metastasis.
At
same
time,
(the
target
organ
metastasis)
also
increased
but
simultaneously
pronounced
decreased
(5
p
0.001)
without
affecting
SOD1
levels.
Conclusion
Liver
tissue
exhibited
inferiority
protection
formation
pro-oxidant
condition
growth,
which
may
prepare
soil
for
Acta Physiologica,
Journal Year:
2024,
Volume and Issue:
240(3)
Published: Jan. 17, 2024
We
tested
the
hypothesis
that
low
testosterone
alters
effects
of
intermittent
hypoxia
(IH)
on
glucose
homeostasis,
hepatic
oxidative
stress,
and
transcriptomic
profile
in
male
mice.
IP Indian Journal of Clinical and Experimental Dermatology,
Journal Year:
2024,
Volume and Issue:
10(3), P. 323 - 327
Published: Sept. 4, 2024
Glutathione
is
one
of
the
non-enzymatic
antioxidants
that
also
involved
in
regulation
cellular
proliferation
and
apoptosis.
The
application
for
skin
lightening
depigmentation
dermatology
started
after
discovery
its
anti-melanogenic
properties.
There
still
a
lack
evidence
regarding
patient
population,
dosage
schedule,
safety
on
long-term
use.
objective
this
study
to
analyze
usage
pattern
from
dermatologists
India
based
their
clinical
experience.
Questionnaire-based
cross-sectional
conducted
amongst
understand
indications,
administration,
safety,
adverse
events
associated
with
therapy.
Data
entry
was
done
Microsoft
Excel
descriptive
statistics
applied.
Seventy-one
responses
were
collected
average
experience
being
5.24
+
7.32
years.
52.11%
these
routinely
prescribe
most
common
use
given
through
oral
route.
preferred
dose
route
250mg
twice
daily
empty
stomach
4
weeks,
topical
2%
(w/w)
applied
once
10
intravenous
1200mg
injection
weekly
over
30
minutes.
Only
3
out
37
prescribing
doctors
have
seen
side
effects.
54%
feel
there
insufficient
data
available
This
shows
current
practices
therapy
by
while
pointing
need
more
studies
be
larger
sample
size
longer
duration
so
standardized
improved
safety.
Problems of Biological Medical and Pharmaceutical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown, P. 59 - 64
Published: Sept. 1, 2024
Introduction.
The
development
of
safe
and
effective
natural
antioxidant
drugs
is
an
important
task
in
the
prevention
treatment
cardiovascular
diseases,
which
are
a
serious
public
health
problem.
Work
Objective
study
antioxidant,
hypoglycemic
hypolipidemic
activities
herbal
extract
hill-growing
saltwort
(Salsola
collina)
comparison
with
effect
standard
agent.
Material
methods.
object
was
"Hepatosol"
biologically
active
food
supplement
based
on
dry
concentrated
aqueous
Salsola
collina
herb
capsules.
patients
under
observation
were
matched
groups
obese
women
men
comparable
age
risk
factors
for
diseases.
Dur-ing
study,
tolerability
possible
adverse
events
recorded
vital
signs
monitored.
results
biochemical
blood
test,
lipid
pro-file,
oxidative
stress
body
composition
analysis
also
evaluated
interpreted.
Results.
It
has
been
found
out
that
parameters
profile
status
obesity
com-plications
taking
3
months
returned
to
normal.
A
positive
reducing
weight
recorded.
safety
good
drug
have
confirmed.
Conclusions.
studies
show
activities.
Due
these
properties,
favorable
diet
therapy
diabetes
mellitus,
loss
use
complex
cardio-vascular
