Towards sustainable agriculture: rhizosphere microbiome engineering

Applied Microbiology and Biotechnology, Journal Year: 2021, Volume and Issue: 105(19), P. 7141 - 7160

Published: Sept. 11, 2021

Language: Английский

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Climate change-associated multifactorial stress combination: A present challenge for our ecosystems

Journal of Plant Physiology, Journal Year: 2022, Volume and Issue: 276, P. 153764 - 153764

Published: July 7, 2022

Humans negatively influence Earth ecosystems and biodiversity causing global warming, climate change as well man-made pollution. Recently, the number of different stress factors have increased, when impacting simultaneously, multiple conditions cause dramatic declines in plant ecosystem health. Although much is known about how plants are affected by each individual stress, recent research efforts diverted into these biological systems respond to several applied together. Studies such "multifactorial combination" concept reported a severe decrease survival microbiome along increasing consistent directional trend. In addition, results concert with studies microbiota natural imposed change. Therefore, all this evidence should serve an important warning order pollutants, create strategies deal increase tolerance stressful combination. Here we review focused on impact abiotic stresses plants, agrosystems including forests microecosystems. mitigate discussed.

Language: Английский

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Strategies to Enhance the Use of Endophytes as Bioinoculants in Agriculture

Horticulturae, Journal Year: 2022, Volume and Issue: 8(6), P. 498 - 498

Published: June 3, 2022

The findings on the strategies employed by endophytic microbes have provided salient information to researchers need maximally explore them as bio-input in agricultural biotechnology. Biotic and abiotic factors are known influence microbial recruitments from external plant environments into tissues. Endophytic exhibit mutualism or antagonism association with host plants. beneficial types contribute growth soil health, directly indirectly. Strategies enhance use of desirable modern agriculture, such that these can be applied individually combined bioinoculants bioprospecting crop breeding systems. Scant is available for shaping microbiome; hence, unravel yield enhancement pathogen suppressiveness become imperative. Therefore, this review focuses microbiome, mechanisms, influencing endophyte recruitment, possible exploration bioinoculants.

Language: Английский

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Environmental microbiome engineering for the mitigation of climate change

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(8), P. 2050 - 2066

Published: Jan. 20, 2023

Environmental microbiome engineering is emerging as a potential avenue for climate change mitigation. In this process, microbial inocula are introduced to natural communities tune activities that regulate the long-term stabilization of carbon in ecosystems. review, we outline process environmental and synthesize key considerations about ecosystem functions target, means sourcing microorganisms, strategies designing inocula, methods deliver factors enable establish within resident community modify an function target. Recent work, enabled by high-throughput technologies modeling approaches, indicate designed from top-down, particularly through directed evolution, may generally have higher chance establishing existing than other historical approaches engineering. We address outstanding questions determinants establishment provide suggestions further research possibilities challenges tool combat change.

Language: Английский

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Phyto-microbiome to mitigate abiotic stress in crop plants

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 2, 2023

Plant-associated microbes include taxonomically diverse communities of bacteria, archaebacteria, fungi, and viruses, which establish integral ecological relationships with the host plant constitute phyto-microbiome. The phyto-microbiome not only contributes in normal growth development plants but also plays a vital role maintenance homeostasis during abiotic stress conditions. Owing to its immense metabolic potential, provides capability mitigate through various mechanisms like production antioxidants, hormones, bioactive compounds, detoxification harmful chemicals toxins, sequestration reactive oxygen species other free radicals. A deeper understanding structure functions complex mediated mitigation would enable utilization for alleviation crop stress-resistant crops. This review aims at exploring potential alleviate drought, heat, salinity heavy metal finding sustainable solutions enhance agricultural productivity. mechanistic insights into phytomicrobiome imparting tolerance have been summarized, that be helpful novel bioinoculants. high-throughput modern approaches involving candidate gene identification target modification such as genomics, metagenomics, transcriptomics, metabolomics, based genetic engineering discussed wake ever-increasing demand climate resilient plants.

Language: Английский

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Plants and endophytes interaction: a “secret wedlock” for sustainable biosynthesis of pharmaceutically important secondary metabolites

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: Nov. 4, 2023

Abstract Many plants possess immense pharmacological properties because of the presence various therapeutic bioactive secondary metabolites that are great importance in many pharmaceutical industries. Therefore, to strike a balance between meeting industry demands and conserving natural habitats, medicinal being cultivated on large scale. However, enhance yield simultaneously manage pest infestations, agrochemicals routinely used have detrimental impact whole ecosystem, ranging from biodiversity loss water pollution, soil degradation, nutrient imbalance enormous health hazards both consumers agricultural workers. To address challenges, biological eco-friendly alternatives looked upon with high hopes where endophytes pitch as key players due their tight association host plants. The intricate interplay endophytic microorganisms has emerged captivating subject scientific investigation, profound implications for sustainable biosynthesis pharmaceutically important metabolites. This review delves into hidden world "secret wedlock" endophytes, elucidating multifaceted interactions underpin synthesis compounds significance plant hosts. Here, we briefly diversity highlight potential role core endomicrobiome. We also propose successful implementation situ microbiome manipulation through high-end techniques can pave way towards more enriched future.

Language: Английский

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Fungal endophyte bioinoculants as a green alternative towards sustainable agriculture

Heliyon, Journal Year: 2023, Volume and Issue: 9(9), P. e19487 - e19487

Published: Aug. 25, 2023

Over the past half century, limited use of synthetic fertilizers, pesticides, and conservation environment natural resources have become interdependent goals sustainable agriculture. These practices support agriculture sustainability with less environmental climatic impacts. Therefore, there is an upsurge in need to introduce compatible booster methods for maximizing net production. The best straightforward strategy explore utilize plant-associated beneficial microorganisms their products. Bioinoculants are bio formulations consisting selected microbial strains on a suitable carrier used enhancement crop Fungal endophytes as bioinoculants confer various benefits host, such protection against pathogens by eliciting immune response, mineralization essential nutrients, promoting plant growth. Besides, they also produce bioactive metabolites, phytohormones, volatile organic compounds. To design bioformulations, transdisciplinary approaches like genomics, transcriptomics, metabolomics, proteomics, microbiome modulation strategies gene editing metabolic reconstruction been explored. studies will refine existing knowledge diversity, phylogeny traits microbes. This help synthesizing consortia evaluating role structural functional elements communities controlled manner. present review summarizes aspects associated fungal capitalizing agricultural outputs, enlists multi-omics techniques understanding modulating mechanism involved endophytism generation new bioformulations providing novel solutions

Language: Английский

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Identification of synthetic consortia from a set of plant‐beneficial bacteria

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(2)

Published: Jan. 30, 2024

Abstract The use of microbial inoculants in agriculture as biofertilisers and/or biopesticides is an appealing alternative to replace or reduce the practice agrochemicals. Plant microbiota studies are revealing different bacterial groups which populating plant microbiomes re‐energising probiotic bacteria (PPB) translational research sector. Some single‐microbial strain bioinoculants have proven valid (e.g., based on Trichoderma , mycorrhiza rhizobia); however, it now recommended consider multistrain consortia since plant‐beneficial effects often a result community‐level interactions microbiomes. A limiting step selection fitting combination strains order accomplish best beneficial effect upon inoculation. In this study, we used subset 23 previously identified and characterised rice‐beneficial colonisers design test series associated experiments aimed identify potential PPB able co‐colonise induce growth promotion. Bacterial were co‐inoculated vitro planta using several methods their co‐colonisation co‐persistence monitored. Results include identification two 5‐strain one 2‐strain displayed growth‐promoting features. Future practical applications microbiome must at identifying can be most effective crop amendments.

Language: Английский

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Cyanobacteria-Mediated Immune Responses in Pepper Plants against Fusarium Wilt

Plants, Journal Year: 2022, Volume and Issue: 11(15), P. 2049 - 2049

Published: Aug. 5, 2022

Research in plant pathology has increasingly focused on developing environmentally friendly, effective strategies for controlling diseases. Cyanobacteria, including Desmonostoc muscorum, Anabaena oryzae, and Arthrospiraplatensis, were applied to Capsicum annuum L. induce immunity against Fusarium wilt. Soil irrigation foliar shoots (FS) application used this investigation. The disease symptoms, index, osmotic contents, total phenol, Malondialdehyde (MDA), hydrogen peroxide (H2O2), antioxidant enzymes (activity isozymes), endogenous hormone content, response stimulation of defense resistance infected plants assessed. Results demonstrated that using all cyanobacterial aqueous extracts significantly reduced the risk infection with oxysporum. One most ways combat was through spraying Arthrospira platensis, oryzae (which provided 95, 90, 69% protection percent, respectively). All metabolic indices increased following extracts. Growth, characteristics, phenols due cyanobacteria. Polyphenol oxidase (PPO) peroxidase (POD) expressions improved cyanobacteria application. Furthermore, treatment by enhanced salicylic acid (SA) Indole-3-Acetic Acid (IAA) while decreasing Abscisic (ABA). pepper recovered from wilt because extract contained many biologically active compounds. seems be an approach relieve toxic influences F. oxysporum as green alternative therapeutic nutrients chemical fungicides.

Language: Английский

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Integrating a tailored recurrent neural network with Bayesian experimental design to optimize microbial community functions

PLoS Computational Biology, Journal Year: 2023, Volume and Issue: 19(9), P. e1011436 - e1011436

Published: Sept. 29, 2023

Microbiomes interact dynamically with their environment to perform exploitable functions such as production of valuable metabolites and degradation toxic for a wide range applications in human health, agriculture, environmental cleanup. Developing computational models predict the key bacterial species factors build optimize are crucial accelerate microbial community engineering. However, there is an unknown web interactions that determine highly complex dynamic behavior these systems, which precludes development based on known mechanisms. By contrast, entirely data-driven machine learning can produce physically unrealistic predictions often require significant amounts experimental data learn system behavior. We develop physically-constrained recurrent neural network preserves model flexibility but constrained consistent show it outperform existing methods prediction certain experimentally measured abundance metabolite concentrations. Further, we present closed-loop, Bayesian design algorithm guide collection by selecting conditions simultaneously maximize information gain target functions. Using bioreactor case study, demonstrate how proposed framework be used efficiently navigate large space identify optimal operating conditions. The methodology offers flexible approach specifically tailored microbiome through sequential informative experiments seek explore exploit

Language: Английский

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