Effects of Altitude on the Digestion Performance, Serum Antioxidative Characteristics, Rumen Fermentation Parameters, and Rumen Bacteria of Sanhe Heifers DOI Creative Commons
Xinyu Zhang, Shuai Huang, Shengli Li

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: April 28, 2022

The production efficiency of dairy cows is affected by altitude, with lower reported at higher altitudes. However, only a few studies have investigated the digestion performance, serum antioxidative characteristics, rumen fermentation and bacteria Sanhe heifers different Therefore, in this study, we explored effects altitude on these aspects heifers. We evaluated apparent digestibility nutrients, parameters, Twenty from same herd managed practice were used. two regions China: 10 fed Hulunbuir City, Inner Mongolia Autonomous Region (119°57′E, 47°17′N; approximately 700 m named LA) Lhasa Tibet (91°06′E, 29°36′N; 3,750 HA). dry matter intake (DMI), average daily gain (ADG), DMI/ADG ratio ( p < 0.05) LA than HA heifers, whereas total tract matter, ether extract, crude proteins group. Compared showed decreased concentrations superoxide dismutase glutathione peroxidase increased concentration hydrogen peroxide 0.05). Altitude did not significantly affect volatile fatty acid rumen, but presented acetate-to-propionate LA. 16S rRNA gene sequencing data that microbial composition. At phylum level, relative abundance Actinobacteria Spirochaetae correlation analysis revealed operational taxonomic units belonging to genus Prevotella_1 correlated DMI. results indicate can influence antioxidant capacity, fermentation, composition study provides insights into adaptation mechanism high-altitude areas.

Language: Английский

The adaptive strategies of yaks to live in the Asian highlands DOI Creative Commons
Xiaoping Jing, Luming Ding, Jianwei Zhou

et al.

Animal nutrition, Journal Year: 2022, Volume and Issue: 9, P. 249 - 258

Published: March 4, 2022

The yak (

Language: Английский

Citations

79
Evaluating the Effect of Dietary Protein–Energy Ratios on Yak Intestinal Microbiota Using High-Throughput 16S rRNA Gene Sequencing DOI Creative Commons
Yanbin Zhu,

Yangji Cidan,

Munwar Ali

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 208 - 208

Published: March 1, 2025

This study investigated the impact of varying dietary protein–energy ratios on intestinal microbiota composition in postpartum weaned female yak. For this study, forty yaks were divided into four groups and provided with different treatments (group FA: high-energy high-protein, FB: low-protein, FC: low-energy FD: control group, 48% alfalfa hay, oat grass, 4% premix) to investigate variations microflora profiles metabolic responses. Rectal fecal samples (n = 24 × 2) collected at day 15 30, from all groups, total DNA was extracted estimate microbial heterogeneity community structures by 16S rRNA sequencing focusing V3–V4 regions, using Illumina Nova Seq 6000 platform. The results revealed a 5,669,645 raw data sequences (3,189,115 2,480,530 respectively). Results showed that FA FB had enhanced protein metabolism diversity, which marked significant increase (p < 0.05) abundance Ruminococcus. Conversely, FD group low level diversity predominance Clostridium Proteobacteria, indicating dysbiosis stress. It concluded imbalanced diets (groups FC FD) upregulated stress-related pathways no favorable shifts, whereas, significantly supported involved amino acids carbohydrate beneficially shifted gut microbiota. These findings emphasize importance supplementation appropriate proportions energy feed promote optimal health functioning, particularly for inhabiting high-altitude is challenging environment.

Language: Английский

Citations

1
The gut microbiome across the lifespan: how diet modulates our microbial ecosystem from infancy to the elderly DOI
Hazem Golshany, Shahinaz Helmy, Nashwa F. S. Morsy

et al.

International Journal of Food Sciences and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 27

Published: Dec. 19, 2024

This comprehensive review examines the impact of dietary patterns on gut microbiome composition and diversity from infancy to old age, linking these changes age-related health outcomes. It investigates how develops across life stages, focusing influence factors. The explores early-life feeding practices, including breastfeeding formula feeding, shape infant microbiota have lasting effects. In elderly individuals, alterations in are associated with increased susceptibility infections, chronic inflammation, metabolic disorders cognitive decline. critical role diet modulating throughout is emphasised, particularly potential benefits probiotics fortified foods promoting healthy ageing. By elucidating mechanisms connecting food systems health, this provides insights into interventions that could enhance resilience improve outcomes lifespan.

Language: Английский

Citations

7
Combined transcriptome and metabolome analysis reveals breed-specific regulatory mechanisms in Dorper and Tan sheep DOI Creative Commons
Yuhao Ma,

Ganxian Cai,

Jianfei Chen

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 17, 2024

Abstract Background Dorper and Tan sheep are renowned for their rapid growth exceptional meat quality, respectively. Previous research has provided evidence of the impact gut microbiota on breed characteristics. The precise correlation between gastrointestinal tract peripheral organs in each is still unclear. Investigating metabolic network intestinal organ potential to improve animal performance enhance economic benefits through regulation metabolites. Results In this study, we identified advantage high fat content sheep. A transcriptome study brain, liver, skeletal muscle, tissues both breeds revealed 3,750 differentially expressed genes (DEGs). PPARGC1A , LPL PHGDH were found be highly Doper, resulting up-regulation pathways related lipid oxidation, glycerophospholipid metabolism, amino acid anabolism. express BSEP LDLR ACHE genes, which up-regulate involved bile transport cholesterol homeostasis. Hindgut analysis 200 accumulated metabolites (DAMs). Purines, pyrimidines, acids, fatty substances more abundant Based combined gene metabolite analyses, have glycine, serine, threonine tryptophan secretion, neuroactive ligand-receptor interaction as key factors contributing differences among breeds. Conclusions This indicates that different exhibit unique characteristics various physiological regulatory methods. upregulate signals threonine, an increase purine pyrimidine substances. This, turn, promotes synthesis acids facilitates body development, a faster rate weight gain. accelerate transport, reduce accumulation intestine, homeostasis muscles. intramuscular fat, improved quality. work adopts joint method multi-tissue metabolome, providing successful case analyzing mechanisms underlying formation traits.

Language: Английский

Citations

6
Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers DOI Creative Commons
Xinyu Zhang, Wei Wang, Zhijun Cao

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 24, 2023

Extreme environments at high altitudes pose a significant physiological challenge to animals. We evaluated the gut microbiome and fecal metabolism in Sanhe heifers from different altitudes.Twenty (body weight: 334.82 ± 13.22 kg, 15-month-old) selected two regions of China: Xiertala Cattle Breeding Farm Hulunbeier, Inner Mongolia [119°57' E, 47°17' N; approximately 700 m altitude, low altitude (LA)] Zhizhao Dairy Cow Lhasa, Tibet [91°06' 29°36' 3,650 (HA)], were used this study. Fecal samples collected differences microbiota metabolomics determined using 16S rRNA gene sequencing metabolome analysis.The results showed that did not significantly affect concentrations volatile fatty acids, including acetate, propionate, butyrate, total acids (p > 0.05). However, affected microbial composition. Principal coordinate analysis based on Bray-Curtis dissimilarity revealed difference between groups = 0.001). At family level, relative abundances Peptostreptococcaceae, Christensenellaceae, Erysipelotrichaceae, Family_XIII lower < 0.05) LA than HA heifers. In addition, Lachnospiraceae, Domibacillus, Bacteroidales_S24-7_group, Bacteroidales_RF16_group, Porphyromonadaceae, Spirochaetaceae higher Metabolomic enrichment 10 metabolic pathways, organismal systems, metabolism, environmental information processing, genetic disease induction. The genera Romboutsia, Paeniclostridium, g_unclassified_f_Lachnospiraceae strongly associated with 28 differential metabolites. This study is first analyze reared provides insights into adaptation mechanism high-altitude areas.

Language: Английский

Citations

12
Comparative analysis of gut fungal composition and structure of the yaks under different feeding models DOI Creative Commons
Yanbin Zhu, Yangji Cidan,

Guangming Sun

et al.

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: June 15, 2023

The yaks that inhabit the Tibetan plateau are a rare breed is closely related to local economic development and human civilization. This ancient may have evolved unique gut microbiota due hypoxic high-altitude environment. susceptible external factors, but research regarding effects of different feeding models on fungal community in remains scarce. In this study, we compared analyzed composition variability among wild (WYG), house-feeding domestic (HFG), grazing (GYG). results revealed Basidiomycota Ascomycota were most preponderant phyla community, regardless models. Although types dominant did not change, their abundances did. Intergroup analysis diversity showed Shannon Simpson indices WYG GYG significantly higher than those HFG. Fungal taxonomic there 20 genera (Sclerostagonospora Didymella) between GYG, 16 (Thelebolus Cystobasidium) Furthermore, proportions 14 (Claussenomyces Papiliotrema) decreased, whereas eight (Stropharia Lichtheimia) increased HFG as GYG. Taken together, study indicated structure differ raised breeding groups.

Language: Английский

Citations

12
Dynamic distribution of gut microbiota in cattle at different breeds and health states DOI Creative Commons
Lei Wang,

Dao‐Yi Wu,

Yu Zhang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 16, 2023

Weining cattle is a precious species with high tolerance to cold, disease, and stress, accounts for large proportion of agricultural economic output in Guizhou, China. However, there are gaps information about the intestinal flora cattle. In this study, high-throughput sequencing were employed analyze (WN), Angus (An), diarrheal (DA), explore potential bacteria associated diarrhea. We collected 18 fecal samples from Weining, including cattle, Healthy Angus, Diarrheal Angus. The results microbiota analysis showed no significant differences diversity richness among groups (p > 0.05). abundance beneficial (Lachnospiraceae, Rikenellaceae, Coprostanoligenes, Cyanobacteria) significantly higher than < pathogens Anaerosporobacter Campylobacteria enriched DA group. Furthermore, Lachnospiraceae was very WN group 0.05), which might explain why less prone This first report on furthering understanding relationship between health.

Language: Английский

Citations

11
Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures DOI Creative Commons
Yangji Cidan, Sijia Lü,

Hongzhuang Wang

et al.

Life, Journal Year: 2024, Volume and Issue: 14(11), P. 1458 - 1458

Published: Nov. 10, 2024

The Jiani yak is a nationally renowned species that known for its meat which rich in various minerals, amino acids, and proteins. rumen microbiota plays critical role gastrointestinal health feed degradation, contributing proteins, lipids, volatile fatty acids (VFAs) essential milk production. However, there limited knowledge about the of free-ranging yaks, especially those with 15 ribs. Rumen fluid samples were collected from yaks 14 (PL) ribs (DL) slaughterhouse County, China. total DNA microorganisms was extracted sequencing. Our results revealed 643,713 656,346 raw sequences DL PL animals, respectively, 611,934 622,814 filtered these two groups. We identified 13,498 Amplicon Sequence Variants (ASVs), 2623 shared between animals. ratio Bacteroidota to Firmicutes differed (3.04) (2.35) Additionally, 6 phyla 21 genera showed significant differences ribs, leading altered functions, 51 35 notably different MetaCyc KEGG pathways, respectively. Hence, differs Therefore, microbiota-related comparative investigations will provide insights into husbandry practices genetic selection strategies their improved productivity harsh environments.

Language: Английский

Citations

4
Comparison of Rumen Fermentation Parameters and Microbiota of Yaks From Different Altitude Regions in Tibet, China DOI Creative Commons
Lulu Han,

Wanchao Xue,

Hanwen Cao

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: Feb. 10, 2022

Rumen microbiota are closely linked to feed utilization and environmental adaptability of ruminants. At present, little is known about the influence different extreme environments on rumen yaks. In this study, 30 ruminal fluid samples from healthy female yaks (average 280 kg BW) in 5-8 years life were collected three regions Tibet, China, compared by gas chromatography high-throughput sequencing. Results showed that propionic acid, butyric total volatile fatty acids significantly (

Language: Английский

Citations

19
The Gut Microbiota Determines the High-Altitude Adaptability of Tibetan Wild Asses (Equus kiang) in Qinghai-Tibet Plateau DOI Creative Commons
Hongjin Liu, Xueping Han, Na Zhao

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: July 18, 2022

It was acknowledged long ago that microorganisms have played critical roles in animal evolution. Tibetan wild asses (TWA, Equus kiang) are the only perissodactyls on Qinghai-Tibet Plateau (QTP) and first national protected animals; however, knowledge about relationships between their gut microbiota host's adaptability remains poorly understood. Herein, 16S rRNA meta-genomic sequencing approaches were employed to investigate microbiota-host associations TWA compared against those of co-resident livestock yak (Bos grunnies) sheep (Ovis aries). Results revealed underwent convergent By contrast, intestinal microflora diverged a direction enabling host subsist sparse low-quality forage. Meanwhile, high microbial diversity (Shannon Chao1 indices), cellulolytic activity, abundant indicator species such as Spirochaetes, Bacteroidetes, Prevotella_1, Treponema_2 supported forage digestion short-chain fatty acid production TWA. enterotype identification analysis showed shifted response for better utilization nitrogen production. Metagenomic plant biomass degrading consortia, genes, enzymes like strains (Prevotella ruminicola, Ruminococcus flavefaciens, albus, Butyrivibrio fibrisolvens, Ruminobacter amylophilus), well carbohydrate metabolism genes (GH43, GH3, GH31, GH5, GH10) (β-glucosidase, xylanase, β-xylosidase, etc.) had significantly higher enrichment Our results indicate can improve through degradation energy maintenance by functions face nutritional deficiencies also provide strong rationale understanding adaptation QTP wildlife when facing harsh feeding environments.

Language: Английский

Citations

19