Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay

Virus Research, Journal Year: 2023, Volume and Issue: 332, P. 199130 - 199130

Published: May 15, 2023

Nipah virus (NiV) is a zoonotic pathogen with airborne transmission and high case fatality rates in humans. There currently no treatment or vaccine against NiV infection approved for humans animals, therefore early diagnosis the key to control any potential outbreaks. In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled CRISPR/Cas13a molecular detection of NiV. The RPA-CRISPR/Cas13a was specific did not cross-react other selected (re)-emerging pathogens. sensitivity can detect as little 103 cp/μL total synthetic cDNA. then validated simulated clinical samples. results could be visualized either fluorescence lateral flow strips convenient field diagnostics, providing useful supplement gold-standard qRT-PCR detecting detections.

Language: Английский

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Discovery of multitarget-directed small molecule inhibitors from Andrographis paniculata for Nipah virus disease therapy: Molecular docking, molecular dynamics simulation and ADME-Tox profiling

Chemical Physics Impact, Journal Year: 2024, Volume and Issue: 8, P. 100493 - 100493

Published: Jan. 24, 2024

Nipah virus (NiV), an emerging highly infectious agent, causes fatal infection to humans through zoonosis, particularly in Asian countries including India. No specific treatment or vaccine is available cure combat the NiV infection. Targetable plant-based computer-assisted drug discovery a novel approach disease. The purpose of current silico study was evaluate potential Andrographis paniculata phytochemicals act as molecules disease by targeting multiple proteins: attachment glycoprotein (NiV-G), nucleoprotein (NiV-N) and phosphoprotein (NiV-P). Six from A. paniculata: andrograpanin (AGNN), andrographidine E (AGDN), andrographin (AGPN), andrographolide (AGLD), deoxyandrographolide (DGLD) neoandrographolide (NGLD) were docked against NiV-G, NiV-N NiV-P. Outcomes molecular docking approved anti-NiV capacity compounds with top binding affinity NGLD all targets NiV-P energies -8.1, -7.7 -6.0 kcal/mol, respectively. dynamic simulation results further validated protein-ligand complexes' stability. Compared phytochemicals, standard antivirals, favipiravir ribavirin, had low efficacy target proteins. Furthermore, phytocompounds displayed acceptable drug-likeness ADME-Tox (absorption, distribution, metabolism, excretion, toxicity) profiles. Overall, present findings can be translated into preclinical clinical investigations inspect mainstream drugs for

Language: Английский

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Advancements and Challenges in Addressing Zoonotic Viral Infections with Epidemic and Pandemic Threats

Viruses, Journal Year: 2025, Volume and Issue: 17(3), P. 352 - 352

Published: Feb. 28, 2025

Zoonotic viruses have significant pandemic potential, as evidenced by the coronavirus pandemic, which underscores that zoonotic infections historically caused numerous outbreaks and millions of deaths over centuries. induce types illnesses in their natural hosts. These are transmitted to humans via biological vectors, direct contact with infected animals or bites, aerosols. continuously evolve adapt human hosts, resulting devastating consequences. It is very important understand pathogenesis pathways associated viral across various hosts develop countermeasure strategies accordingly. In this review, we briefly discuss advancements diagnostics therapeutics for infections. provides insight into recent outbreaks, dynamics, licensed vaccines, well vaccine candidates progressing clinical investigations. Despite advancements, challenges persist combating due immune evasion, unpredicted complexity responses. Most these lack effective treatments relying entirely on supportive care preventive measures. Exposure animal reservoirs, limited access, insufficient coverage further pose efforts. This review highlights critical need ongoing interdisciplinary research collaboration strengthen preparedness response against emerging infectious threats.

Language: Английский

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Henipavirus zoonosis: outbreaks, animal hosts and potential new emergence

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 17, 2023

Hendra virus (HeV) and Nipah (NiV) are biosafety level 4 zoonotic pathogens causing severe often fatal neurological respiratory disease. These agents have been recognized by the World Health Organization as top priority expected to result in future outbreaks. HeV has caused sporadic infections horses a small number of human cases Australia since 1994. The NiV Malaysia genotype (NiV-M) was responsible for 1998–1999 epizootic outbreak pigs with spillover humans Singapore. Since 2001, Bangladesh (NiV-B) predominant strain leading outbreaks almost every year India, hundreds humans. natural reservoir hosts fruit bats, which carry viruses without clinical manifestation. transmission pathways henipaviruses from bats remain poorly understood. Transmissions bridged an intermediate animal host, amplifies spreads Horses known NiV-M epidemic Singapore, respectively. During NiV-B Bangladesh, following initial thought be through consumption date palm sap, spread infection largely human-to-human transmission. Spillover recent India is less understood, primary route bat case(s) unknown no host established. This review aims provide concise update on epidemiology covering their previous current emphasis potential role livestock disease Also included up-to-date summary newly emerging henipa-like hosts. In these contexts we discuss knowledge gaps new challenges field propose directions.

Language: Английский

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Possible high risk of transmission of the Nipah virus in South and South East Asia: a review

Tropical Medicine and Health, Journal Year: 2023, Volume and Issue: 51(1)

Published: Aug. 10, 2023

Abstract Nipah virus (NiV) is a zoonotic, single-stranded RNA from the family Paramyxoviridae, genus Henipavirus . NiV biosafety-level-4 pathogen that mostly spread by Pteropus species, which serve as its natural reservoir host. one of major public health challenges in South and East Asia. However, few molecular studies have been conducted to characterise specific region. The main objective this review understand epidemiology, pathogenesis, surveillance, transmission dynamics, genetic diversity, host, clinical characteristics, phylogenetics NiV. Asian nations experienced outbreaks. Phylogenetic analysis confirmed two primary clades are circulation. In humans, causes severe respiratory illness and/or deadly encephalitis. mainly diagnosed ELISA along with PCR. Therefore, we recommend governments region support One Health approach reducing risk zoonotic disease their respective countries.

Language: Английский

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Nipah virus strikes Kerala: recent cases and implications

The Egyptian Journal of Internal Medicine, Journal Year: 2024, Volume and Issue: 36(1)

Published: Jan. 29, 2024

Abstract The Nipah virus, a highly pathogenic zoonotic pathogen, has once again surfaced in the state of Kerala, India, with recent cases reported 2023. This article delves into epidemiological details these cases, shedding light on two fatalities that occurred September 2023 and August as well suspected have undergone testing. known for its severe neurological respiratory disease manifestations, remains formidable health threat due to lack specific treatments or vaccines. government’s response including mass testing quarantine measures, is discussed, drawing lessons from past outbreaks region. Kerala experienced multiple virus since 2018, prompting closer examination environmental factors, such deforestation urbanization, which increase risk transmission. underscores global implications resurgence emphasizes importance preparedness vigilance face emerging infectious diseases. learned Kerala’s experience provide valuable insights public professionals policymakers worldwide.

Language: Английский

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Exploiting cellulose-based hydrogels for sustainable, intelligent wearables in pandemic preparedness and control

European Polymer Journal, Journal Year: 2024, Volume and Issue: 212, P. 113041 - 113041

Published: April 15, 2024

Language: Английский

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Henipavirus Immune Evasion and Pathogenesis Mechanisms: Lessons Learnt from Natural Infection and Animal Models

Viruses, Journal Year: 2022, Volume and Issue: 14(5), P. 936 - 936

Published: April 29, 2022

Nipah henipavirus (NiV) and Hendra (HeV) are zoonotic emerging paramyxoviruses causing severe disease outbreaks in humans livestock, mostly Australia, India, Malaysia, Singapore Bangladesh. Both bat-borne viruses humans, their mortality rates can reach 60% the case of HeV 92% for NiV, thus being two deadliest known humans. Several factors, including a large cellular tropism wide potential, con-tribute to high pathogenicity. This review provides an overview NiV pathogenicity mechanisms summary interactions with immune systems different host species, natural hosts bats, spillover-hosts pigs, horses, as well experimental animal models. A better understanding between henipaviruses could facilitate development new therapeutic strategies vaccine measures against these re-emerging viruses.

Language: Английский

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Cannabis sativa: 2023-Outbreak and Re-emergence of Nipah virus (NiV) in India: Role of Hemp oil

GSC Biological and Pharmaceutical Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 063 - 077

Published: Oct. 10, 2023

This review paper highlights and updates the outbreak of Nipah virus (NiV), an emerging zoonotic bat borne that can cause severe respiratory illness deadly encephalitis in humans. (NiV) was first reported 1998 from Malaysia then recorded Bangladesh, India, Philippines, Singapore Thailand. A recent fifth during August-September 2023 Kerala State, India brought this emerging-re-emerging into spotlight again. Due to lack vaccines drugs with proven effectiveness against treatment patients is limited supportive prophylactic. Fruit bats are main reservoir for virus, which disease humans animals. be transmitted or livestock humans, typically via contaminated food (fruit raw date palm sap), person-to-person through secretions. Hemp seed oil has gained remarkable popularity due its perceived therapeutic properties, particularly antiviral properties potential health benefits. During local traditional healers used hemp as mouth wash controlling throat infections, head ache, vomiting suggested consumption seeds functional food. However, clinical trials scientific data supporting activity Cannabis (CBD oil) lacking. Therefore, further research should focus on exploring molecular mechanisms warranted. Multicenter performed validate efficacy alone form formulations viral infections.

Language: Английский

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Establishment of a novel minigenome system for the identification of drugs targeting Nipah virus replication

Journal of General Virology, Journal Year: 2024, Volume and Issue: 105(1)

Published: Jan. 5, 2024

Nipah virus (NiV) is a deadly zoonotic pathogen with high potential to cause another pandemic. Owing biosafety concerns, studies on living NiV must be performed in level 4 (BSL-4) laboratories, which greatly hinders the development of anti-NiV drugs. To overcome this issue, minigenome systems have been developed study viral replication and screen for antiviral This aimed develop two (transient stable expression) based helper cell line expressing P, N L proteins required initiate RNA replication. Stable cells were resistant ribavirin, remdesivir favipiravir but sensitive interferons. Cells transient system ribavirin suitable drug screening. Our demonstrates feasible effective platform studying shows great high-throughput screening BSL-2 laboratory environment.

Language: Английский

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