Spatial patterns of Hyalomma marginatum -borne pathogens in the Occitanie region (France), a focus on the intriguing dynamics of Rickettsia aeschlimannii

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(8)

Published: July 16, 2024

Hyalomma marginatum is an invasive tick species recently established in mainland southern France. This known to host a diverse range of human and animal pathogens. While information about the dynamics these pathogens crucial assess disease risk develop effective monitoring strategies, few data on spatial are currently available. We collected ticks 27 sites Occitanie region characterize patterns H. marginatum-borne Several have been detected: Theileria equi (9.2%), orientalis (0.2%), Anaplasma phagocytophilum (1.6%), marginale (0.8%), Rickettsia aeschlimannii (87.3%). Interestingly, we found clustered distribution for pathogen R. between two geographically isolated areas with infection rates bacterial loads significantly lower Hérault/Gard departments (infection rate 78.6% average) compared Aude/Pyrénées-Orientales 92.3% average). At smaller scale, varied from one site another, ranging 29% 100%. Overall, such high (87.3% maternal transmission might suggest role as symbiont marginatum. Further studies thus needed understand both status ticks.IMPORTANCETicks obligatory hematophagous arthropods that transmit medical veterinary importance. Pathogen infections cause serious health issues humans considerable economic loss domestic animals. Information presence their public health. Analyzing tick-borne region, our results highlight clear strengthen postulate it essential strategies consider scale better circulation

Language: Английский

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Genomic and Proteomic Analyses of Bacterial Communities of Ixodes scapularis Ticks from Broome County, New York

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 258 - 258

Published: Jan. 24, 2025

The microbial communities of Ixodes scapularis, the primary vector Lyme disease in North America, exhibit regional variations that may affect pathogen transmission and competence. We analyzed bacterial I. scapularis ticks collected from Broome County, New York, using 16S rRNA gene sequencing (18 ticks) as well mass spectrometry-based proteomics (36 ticks). According to analysis, endosymbiont Rickettsia buchneri was most abundant species, with significantly higher (p = 0.0011) abundance females (54.76%) compared males (31.15%). detected Borreliella burgdorferi 44.44% Anaplasma phagocytophilum two nymphs but high relative abundances (12.73% 46.46%). Male exhibited diversity, although community composition showed no significant clustering by sex or life stage. Co-occurrence analysis revealed negative associations between R. Pseudomonas 0.0245), B. burgdorferi. Proteomic identified 12 buchneri-specific proteins, additionally detecting protozoan Babesia microti 18.18% females. These findings provide first comprehensive characterization microbiomes Southern Tier region York suggest broader distribution across tick stages than previously recognized, potential implications for dynamics.

Language: Английский

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A systematic review of the microbiome of Hyalomma Koch, 1844 ticks using next-generation sequencing of the 16S ribosomal RNA gene

Veterinary World, Journal Year: 2025, Volume and Issue: unknown, P. 1090 - 1100

Published: Jan. 1, 2025

Background and Aim: Ticks are critical vectors of pathogens affecting humans livestock globally. The microbiome ticks, comprising diverse bacterial communities, plays a crucial role in tick biology vector competence. Hyalomma ticks prominent the Middle East North Africa (MENA) region known carriers significant pathogens. This study aimed to systematically evaluate existing literature regarding composition MENA region, identify predominant genera, highlight knowledge gaps. Materials Methods: A systematic search was conducted using four databases: ScienceDirect, PubMed, Google Scholar, Scopus. covered studies published between 2014 2024 employing 16S ribosomal RNA gene sequencing analyze microbiomes within region. Studies not fulfilling these criteria were excluded through independent assessment by two authors. Results: Out 1,220 screened articles, seven met inclusion criteria, involving five species: dromedarii, anatolicum, excavatum, marginatum, scupense. Most (57.14%) focused on H. primarily collected from camels. geographical distribution included United Arab Emirates (42.86%), Saudi Arabia (28.57%), Iran (14.29%), Tunisia (14.29%). Common genera identified across multiple Acinetobacter, Bacillus, Flavobacterium, Francisella, Rickettsia, Staphylococcus, Pseudomonas, Corynebacterium. However, substantial gaps noted, particularly concerning variations related lifecycle stages, host interactions, temporal dynamics, extensive geographic coverage Conclusion: review underscores presence key revealing their potential roles pathogen transmission. Major research include limited scope, insufficient exploration variation life host-specific environmental factors influencing microbial communities. Addressing comprehensive, longitudinal, multi-regional is imperative for improving public health strategies developing targeted tick-control methods. Keywords: sequencing, Hyalomma, microbiome, ticks.

Language: Английский

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Effects of Purified Tick Saliva Toxins on Various Bio-Molecules in Blood Serum, Liver and Muscles of Albino Mice

American Journal of Animal and Veterinary Sciences, Journal Year: 2024, Volume and Issue: 19(1), P. 43 - 57

Published: Jan. 1, 2024

In the present study, salivary toxins from Rhipicephalus microplus were isolated and purified on a Sepharose CL 6B 200 gel column at constant flow rate with regular fractions of 5 mL/min. Pooled lyophilized LD50 was then determined in albino mice. The value protein toxin found 36.11 mg/kg body weight To determine physiological effects, sub-lethal dose 40-80% 24 h injected into different biological assays, total serum protein, free amino acids, uric acid, cholesterol, pyruvic lipids glucose levels stages. addition, effects tick saliva observed rectus abdomens, gastrocnemius, muscles, atria ventricles rats muscle glycogen. A significant increase bimolecular activity serum, liver group showed an content 79.70% while all increased correspondingly. Toxins broke down glycogen stores liver, abdominal stomach significantly reduced concentrations (67-82%) These due to presence low molecular proteins that act membrane interfere cell function could be used for drug development target molecules generation transmission-blocking vaccines or anti-tick vaccines.

Language: Английский

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Tick symbiosis

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 62, P. 101163 - 101163

Published: Jan. 18, 2024

Language: Английский

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Ixodes Ticks – Carriers of Pathogens of Vector-Borne Infections

International Journal Of Pharmaceutical Research And Allied Sciences, Journal Year: 2024, Volume and Issue: 13(1), P. 74 - 82

Published: Jan. 1, 2024

Ixodes ticks are known as carriers of pathogens many infectious diseases with natural foci. 12 species carrying the virus Crimean-Congo hemorrhagic fever and lumpy skin disease (dermatitis nodulares) cattle were found in foothill zone Kabardino-Balkaria. In Republic Dagestan, Hyalomma. p. plumbeum taileriosis (13.6%), incidence animals is 24.4%. Kaluga region, infection I. ricinus

Language: Английский

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Detection of pathogenic bacteria in ticks from Isiolo and Kwale counties of Kenya using metagenomics

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(4), P. e0296597 - e0296597

Published: April 30, 2024

Ticks are arachnid ectoparasites that rank second only to mosquitoes in the transmission of human diseases including bacteria responsible for anaplasmosis, ehrlichiosis, spotted fevers, and Lyme disease among other febrile illnesses. Due paucity data on transmitted by ticks Kenya, this study undertook a bacterial metagenomic-based characterization collected from Isiolo, semi-arid pastoralist County Eastern Kwale, coastal with monsoon climate southern Kenyan border Tanzania. A total 2,918 belonging 3 genera 10 species were pooled screened study. Tick identification was confirmed through sequencing Cytochrome C Oxidase Subunit 1 ( COI ) gene. Bacterial 16S rRNA gene PCR amplicons obtained above samples sequenced using MinION (Oxford Nanopore Technologies) platform. The resulting reads demultiplexed Porechop, followed trimming filtering Trimmomatic before clustering Qiime2-VSearch. SILVA database pretrained naïve Bayes classifier used classify Operational Taxonomic Units (OTUs) taxonomically. clinical interest detected tick assays as follows: Rickettsia spp . 59.43% pools, Coxiella burnetii 37.88%, Proteus mirabilis 5.08%, Cutibacterium acnes 6.08%, Corynebacterium ulcerans 2.43%. These query fever (Q-fever), urinary tract infections, skin soft tissue eye diphtheria-like infections humans, respectively. P , Isiolo. Additionally, sequences allowed strain levels some pools. Diversity analysis revealed had high Alpha diversity but differences between microbiomes three studied not significant. detection commonly associated flora suggests may have contact humans potentially exposing them infections. findings highlight need further investigation into viability these competency transmit them. Clinicians high-risk areas also be appraised include Rickettsial Q-fever part their differential diagnosis.

Language: Английский

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What do we know about the microbiome of I. ricinus?

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: Nov. 16, 2022

I. ricinus is an obligate hematophagous parasitic arthropod that responsible for the transmission of a wide range zoonotic pathogens including spirochetes genus Borrelia, Rickettsia spp., C. burnetii , Anaplasma phagocytophilum and Francisella tularensis which are part tick´s microbiome. Most studies focus on “pathogens” only very few elucidate role “non-pathogenic” symbiotic microorganisms in . While most members microbiome leading intracellular lifestyle, they able to complement nutrition stress response having great impact survival pathogens. The composition not consistent can be tied environment, tick species, developmental stage, or specific organ tissue. Ovarian tissue harbors stable consisting mainly but exclusively endosymbiotic bacteria, while digestive system rather unstable, together with salivary glands, mostly comprised prevalent endosymbionts found ticks Ricketsiella Coxiella -like endosymbionts, Spiroplasma spp. Candidatus Midichloria Since modify ticks’ behavior, such as mobility, feeding saliva production, results increased rates, we aimed potential, tight relationship, interaction between bacteria Here show provide different types vitamin B (B2, B6, B7, B9) essential eukaryotic organisms. Furthermore, hypothesize Wolbachia bacterial pathogen A. supported by itself since coinfection ixodetis provides complete metabolic pathway folate biosynthesis necessary DNA synthesis cell division. Manipulation could present perspective way control regulation spread emerging

Language: Английский

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Another tick bites the dust: exploring the association of microbial composition with a broad transmission competence of tick vector species

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Oct. 6, 2023

ABSTRACT Ticks harbor and transmit many different pathogens. While some species are competent vectors of a single pathogen, mono-competent (MCV), other proven to be several pathogens, pluri-competent (PCV). Such difference in vector competence might related the microbiome. To better comprehend its influence on ticks for one or data-driven approach using publicly available databases was applied bacterial 16S rRNA from MCV PCV tick species. Alpha beta diversity, co-occurrence networks, functional profiles were analyzed. A differential analysis performed identify genera associated with ticks. These presented higher richness, composition showed significant between The bacteria demonstrated fewer correlations within each comparison revealed 14 species, such as Rickettsia , Staphylococcus Corynebacterium . Using 24 differently abundant genera, samples another data set could classified into either high accuracy concordance. Moreover, pathway regulation reactive oxygen detoxification, β-Lactam resistance, dTDP-L-rhamnose biosynthesis participating tick-borne findings enlighten our understanding community’s role species’ broad competence. IMPORTANCE Some more than pathogen while are, until now, known only any pathogen. pathogens microbiome, what differentiates these two groups help us control diseases aiming at that contribute groups, seemed Our results highlight differences when they divided based number transmit. first step relationship it transmits.

Language: Английский

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Spatial patterns ofHyalomma marginatum-borne pathogens in the Occitanie region (France), a focus on the intriguing dynamics ofRickettsia aeschlimannii

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 5, 2024

ABSTRACT Hyalomma marginatum is an invasive tick species recently established in mainland southern France. This known to host a diverse range of human and animal pathogens such as Rickettsia aeschlimannii , Theileria equi Anaplasma phagocytophilum marginale Ehrlichia minasensis Crimean-Congo hemorrhagic fever West Nile virus. While information about the dynamics these crucial assess disease risk develop effective monitoring strategies, few data on spatial are currently available. We thus collected ticks 27 sites Occitanie region characterize patterns H. - borne pathogens. Several have been detected: (9.2%), orientalis (0.2%), (1.6%), (0.8%) (87.3%). Interestingly, we found clustered distribution for pathogen R. between two geographically isolated areas with infection rates bacterial loads significantly lower Hérault/Gard departments (infection rate 78.6% average) compared Aude/Pyrénées-Orientales 92.3% average). At smaller scale, varied from one site another, ranging 29% 100%. Overall, high (87.3% maternal transmission might suggest role symbiont . Moreover, identified pathogen, results also question its pathogenic status humans given low number cases. Further studies needed understand both ticks. IMPORTANCE Ticks obligatory hematophagous arthropods which transmit medical veterinary importance. Their infections cause serious health issues considerable economic loss domestic animals. Information presence their public health. Analysing tick-borne regions Occitanie, our highlight clear -borne strengthen postulate that it essential strategies consider scale better circulation

Language: Английский

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