Peganum harmala Polysaccharide Mitigates LPS‐Induced Inflammatory Response in Macrophages by Activating Autophagy Pathway

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 1, 2025

ABSTRACT Peganum harmala, a member of the Zygophyllaceae family, is known for its diverse biological activities, including anti‐inflammatory properties. The mechanisms through which P. harmala polysaccharide (LTP) induces autophagy, however, remain largely unexplored. This study aims to elucidate role LTP in autophagy induction and efficacy mitigating inflammation within macrophages. Autophagosome formation was evaluated using GFP‐LC3 vectors, LC3‐II levels induced by were analyzed laser scanning confocal microscopy. Western blotting assessed expression autophagy‐related proteins phosphorylation state p70S6K NRK cells, treated both with without LTP, alongside inducers inhibitors. Additionally, RAW264.7 cells 1 μg/mL lipopolysaccharides (LPS), followed ELISA assays quantify inflammatory markers. study's outcomes demonstrate that facilitates an increase autophagic activity, as evidenced enhanced reduced p62 effect mediated activation AMPK‐mTOR signaling pathway, inhibiting autophagosome–lysosome fusion process. In vitro experiments LPS showed markedly decreased TNF‐α IL‐6. Our findings indicate effectively reduces LPS‐stimulated macrophages promoting via mTOR‐dependent mechanism.

Language: Английский

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5mC DNA methylation modification-mediated regulation in tissue functional differentiation and important flavor substance synthesis of tea plant (Camellia sinensis L.)

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(8)

Published: June 10, 2023

In plants, 5mC DNA methylation is an important and conserved epistatic mark involving genomic stability, gene transcriptional regulation, developmental abiotic stress response, metabolite synthesis, etc. However, the roles of modification (5mC methylation) in tea plant growth development (in pre-harvest processing) flavor substance synthesis pre- post-harvest processing are unknown. We therefore conducted a comprehensive analysis four key tissues (root, leaf, flower, fruit) two processed leaves during oolong processing. found that differential among closely related to tissue functional differentiation genes expressed tissue-specifically, responsible for tissue-specific functions, maintain relatively low levels relative non-tissue-specifically genes. Importantly, hypomethylation modifications CsAlaDC TS/GS roots provided molecular basis dominant theanine roots. addition, integration methylationomics, metabolomics, transcriptomics revealed content changes metabolites were associated with transcription level corresponding genes, transcript these strictly regulated by methylation. further report some methylation, which can effectively explain aroma metabolites, including α-farnesene, nerolidol, lipids, taste substances such as catechins. Our results not only highlight processing, but also provide epimutation-related targets future improvement quality or breeding whole-tissue high-theanine varieties.

Language: Английский

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Metabolomic and Proteomic Profile of Dried Hop Inflorescences (Humulus lupulus L. cv. Chinook and cv. Cascade) by SPME-GC-MS and UPLC-MS-MS

Separations, Journal Year: 2022, Volume and Issue: 9(8), P. 204 - 204

Published: Aug. 6, 2022

Hop (Humulus lupulus L.) is grown mainly for the production of beer. The flowers female plant give it bitter taste and pungent aroma. There are a large number hop varieties differing in their α-acid content, essential oil levels odor profiles. Aside from use brewing, more recently, hops have been used pharmacological properties its derivatives that great importance to pharmaceutical industry. known fairly complex chemistry characterized by presence variety sesquiterpenoids, diterpenoids triterpenoids, phytoestrogens flavonoids. Additionally, considering countless applications sector recent years, chemical characterization different cultivars better identify source specific secondary metabolites. For this purpose, dried inflorescences two cultivars, Chinook Cascade, were investigated using Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry Liquid Chromatography-Tandem Mass (SPME-GC-MS LC-MS-MS) describe metabolomic proteomic profile. Furthermore, thanks an in-depth statistical survey, was possible carry out comparative study highlighting interesting implications deriving investigative study.

Language: Английский

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Effectiveness of phytoproducts against pathogenic free-living amoebae - A scoping and critical review paving the way toward plant-based pharmaceuticals

Fitoterapia, Journal Year: 2025, Volume and Issue: 182, P. 106404 - 106404

Published: Feb. 6, 2025

Language: Английский

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An Update to Alkaloids Derived from Tryptophan: Harmine and Relative Alkaloids

Springer eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 33

Published: Jan. 1, 2025

Language: Английский

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In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4966 - 4966

Published: May 2, 2024

Herpes simplex virus (HSV) infections are highly widespread among humans, producing symptoms ranging from ulcerative lesions to severe diseases such as blindness and life-threatening encephalitis. At present, there no vaccines available, some existing antiviral treatments can be ineffective or lead adverse effects. As a result, is need for new anti-HSV drugs. In this report, the in vitro effect of 9,9′-norharmane dimer (nHo-dimer), which belongs β-carboline (βC) alkaloid family, was evaluated. The exhibited virucidal properties did not impede either attachment penetration steps viral particles. only exerted under constant presence incubation media, mechanism action found involve later events infection. Analysis fluorescence lifetime imaging data showed that nHo-dimer internalized well into cells when present extracellular medium, with preferential accumulation perinuclear organelles including mitochondria. After washing host fresh medium free nHo-dimer, signal decreased, suggesting partial release compound cells. This agrees observation solely manifested consistently media.

Language: Английский

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Synthesis and investigation of nano-biologic structures’ effect on soil pathogenic fungi in maize

Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 133, P. 102336 - 102336

Published: June 20, 2024

Language: Английский

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Alkaloids from the entheogenic plant Peganum harmala

Australian Journal of Chemistry, Journal Year: 2023, Volume and Issue: 76(5), P. 264 - 278

Published: May 4, 2023

Entheogenic natural products (e.g., psylocybin and dimethyltryptamine) are emerging as effective therapeutics to treat debilitating mood disorders that unresponsive conventional treatment. The detailed evaluation of psychotropic plants will conceivably lead the discovery structurally distinct entheogens may offer improved or complementary medicinal properties classic entheogens. plant Peganum harmala has a rich history in traditional medicine, with consumption inducing host central nervous system (CNS) symptoms, including hallucinations. Given alkaloids uniquely capable altering CNS physiology owing their ability cross blood–brain barrier, product(s) responsible for entheogenic likely hidden its diverse alkaloid profile. Herein, an overview 160 isolated from P. is provided. Remarkably, bioactivity data scarce, limited inhibition monoamine oxidases cholinesterases few cases, majority having no reported at all. As none have been detected harmala, this collection provides useful reference point search unique

Language: Английский

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Characterization and identification of antimicrobial compounds from endophytic Fusarium incarnatum isolated from Cymbidium orchids

International Microbiology, Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 17, 2023

Language: Английский

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Screening and Characterization of Streptomyces spp. Isolated from Soil Producing a Potential Inhibitors of the Proteus mirabilis

Journal of Biotechnology & Bioinformatics Research, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 10

Published: Dec. 7, 2023

Proteus mirabilis is a common cause of urinary tract infections and other nosocomial that can lead to severe health consequences. Despite the availability various antibiotics, increasing prevalence multidrug-resistant (MDR) strains has necessitated development novel therapeutic compounds. The ability species produce wide variety secondary metabolites, such as antibiotics Streptomyces, immunosuppressants, antivirals, well known. In this study, Streptomyces strain was isolated from soil near saltwater bodies, its metabolic extract evaluated for antimicrobial activity against mirabilis. isolate demonstrated significant mirabilis, with minimum inhibitory concentration (MIC) 34 µg/mL. potential confirmed through additional examination metabolite using deterministic assays, nucleic acid protein release time-kill curve assay. Molecular identification showed similarity triticirhizae. GC-MS analysis revealed presence compounds Octadecanoic n-Hexadecanoic acid, which are frequently associated potent activity. HR-LCMS Neomycin, Polymyxin B, Maculosin, also have These findings strongly indicate extracts compound

Language: Английский

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