Plant Stress, Journal Year: 2024, Volume and Issue: unknown, P. 100720 - 100720
Published: Dec. 1, 2024
Language: Английский
Life, Journal Year: 2025, Volume and Issue: 15(4), P. 554 - 554
Published: March 28, 2025
In the present study, strain Rhizopus oryzae EMM was isolated from germinated autochthonous red maize seeds, which were harvested in a region of San Diego-Buenavista, Papalotla, Tlaxcala, Mexico, where cobs with fungal infections have been observed. This caused wilting seedlings. Pseudomonas protegens EMM-1 tested for its ability to inhibit R. EMM, both culture media and association plantlets. P. inhibited growth under all conditions explored. The associated plants evaluated hydroponic system vermiculite. systems, strongly EMM. dry weight root infected inoculated increased 0.43 g, while that only reached just 0.19 g conditions. However, no differences observed vermiculite aerial greater than These results indicate inhibits infection by thereby improving plant growth. Moreover, genome analysis revealed presence several genes potentially encode antimicrobial compounds, could strengthen potential use as biocontrol agent plants.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 145 - 172
Published: Jan. 1, 2025
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
Citations
0
Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102720 - 102720
Published: May 1, 2025
Language: Английский
Citations
0
Sustainability, Journal Year: 2024, Volume and Issue: 16(5), P. 2006 - 2006
Published: Feb. 29, 2024
Microbial interactions, which regulate the dynamics of eco- and agrosystems, can be harnessed to enhance antagonism against phytopathogenic fungi in agriculture. This study tests hypothesis that plant growth-promoting rhizobacteria (PGPR) also potential biological control agents (BCAs). Antifungal activity assays potentially were caried out using cultures cell-free filtrates nine PGPR strains previously isolated from agricultural soils. Cultures Bacillus sp. BS36 inhibited growth Alternaria AF12 Fusarium AF68 by 74 65%, respectively. Cell-free same strain both 54 14%, Furthermore, co-cultivation with Pseudomonas BS95 target improved their antifungal activity. A subsequent metabolomic analysis Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) identified fengycin- surfactin-like lipopeptides (LPs) filtrates, could explain The co-production multiple families LPs is an interesting feature practical applications. These results highlight work as a BCA need for more integrative approaches develop biocontrol tools accessible adoptable farmers.
Language: Английский
Citations
3
Microbiological Research, Journal Year: 2023, Volume and Issue: 272, P. 127396 - 127396
Published: April 28, 2023
Language: Английский
Citations
9
Antibiotics, Journal Year: 2024, Volume and Issue: 13(7), P. 597 - 597
Published: June 27, 2024
Pseudomonas bacteria are renowned for their remarkable capacity to synthesize antibiotics, namely mupirocin, gluconic acid, pyrrolnitrin, and 2,4-diacetylphloroglucinol (DAPG). While these substances extensively employed in agricultural biotechnology safeguard plants against harmful fungi, potential human medicine healthcare remains highly promising common science. However, the challenge of obtaining stable producers that yield higher quantities antibiotics continues be a pertinent concern modern biotechnology. Although interest has persisted over past century, many uncertainties still surround regulation biosynthetic pathways compounds. Thus, present review comprehensively studies genetic organization biosynthesis provides comprehensive summary antibiotic pseudomonas strains, appealing both molecular biologists biotechnologists. In addition, attention is also paid application plant protection.
Language: Английский
Citations
2
Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127887 - 127887
Published: Aug. 30, 2024
Language: Английский
Citations
2
Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14
Published: Dec. 7, 2023
Gray mold, caused by Botrytis cinerea is a major cause of post-harvest rot fresh fruits and vegetables. The utilization selected microorganisms as biocontrol agents promising alternative to effectively control gray mold on tomatoes. current study was conducted explore potential mechanisms the Pseudomonas strain infections Among 8 tested bacterial isolates, protegens ML15 demonstrated antagonistic activity . Moreover, P. exhibited production siderophores, hydrogen cyanide, ammonia, exopolysaccharides, lipase, biosurfactant, 2,4-diacetylphloroglucinol, several other antifungal compounds, such 1-tetradecanol, cyclododecane, 2,4-di-tert-butylphenol, 2-methyl-1-hexadecanol. A comprehensive genomic analysis unravels 18 distinct genetic regions with for biosynthesizing secondary metabolites, known their pivotal role in responses against plant pathogens. In vivo , experiments showed that both culture suspension cell-free supernatant significantly reduced fungal growth (53.0 ± 0.63%) mitigated disease development (52.8 1.5%) cherry tomatoes at four days post- B inoculation. During infection, application resulted augmentation total antioxidant, phenolic content, ascorbic acids content. Thus, our results suggested ML15’s agent B. -induced postharvest tomato decay achieved through secretion substances, induction defense responses, inhibition mycelial These findings provide significant contribution ongoing search alternative, eco-friendly methods controlling products. could help reduce reliance chemical fungicides promote sustainable agriculture practices.
Language: Английский
Citations
6
Rhizosphere, Journal Year: 2024, Volume and Issue: 30, P. 100897 - 100897
Published: May 16, 2024
The search for new biological products with a positive impact on crop performance is typically initiated by laboratory based in vitro assays. However, live plants and their associated microbes are often removed from testing assays as way to reduce complexity (variation) facilitate molecular techniques the pursuit of uncovering mode-of-action (MoA) mechanisms. Nevertheless, when studying candidates intended use agriculture, it essential incorporate this validate mechanisms under conditions close situ possible order understand capacities MoA biologicals application environments. To address paradox, we have developed high-capacity early-stage plant assay that incorporates non-sterile while also enabling investigations, can be conducted laboratories without greenhouse facilities. design features grown 8-chamber transparent boxes allow multiplex imaging increased replicates greater statistical power. box allows visualization shoots, roots, tagged-microbes, or visible substrates, further non-destructive access shoots roots sampling. held racks hold eight during growth 19 17 cm space, increasing throughput > 670 per m2 easing logistical challenges Furthermore, support various levels microbial option select medium meets experimental objectives, well using sterile seeds. A script-based post-imaging quantification was automate image processing individual readings, confidence. As proof concept, system evaluate biocontrol potential Pseudomonas protegens biostimulation koreensis, both cases able show statistically significant differing biomass between treatments these closer-to-nature conditions. We demonstrate suitable paired investigations performing metabolomics qPCR DNA directly explore chemical MoA, confirm survival P. strains role improved phenotype. In conclusion, study presents modular enables functional biostimulant planta. This novel assaying saves time, reduces human error, provides quantitative planta data, used therefore believe potent bridges testing, will expedite discovery superior next-generation agriculture.
Language: Английский
Citations
1