Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract

Food Bioengineering, Journal Year: 2024, Volume and Issue: 3(1), P. 41 - 64

Published: March 1, 2024

Abstract Probiotics have become increasingly popular over the past two decades due to continuously expanding scientific evidence indicating their beneficial effects on human health. Therefore, they been applied in food industry produce functional food, which plays a significant role health and reduces disease risk. However, maintaining viability of probiotics targeting successful delivery gastrointestinal tract remain challenging tasks applications. Specifically, this paper reviews potentially properties probiotics, highlighting use challenges application associated benefits. Of foremost importance, also explores potential underlying molecular mechanisms enhanced effect epithelial cells, including discussion various surface adhesion‐related proteins probiotic cell that facilitate colonization.

Language: Английский

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Microencapsulation and Probiotic Characterization of Lactiplantibacillus plantarum LM-20: Therapeutic Application in a Murine Model of Ulcerative Colitis

Nutrients, Journal Year: 2025, Volume and Issue: 17(5), P. 749 - 749

Published: Feb. 20, 2025

Background: Microencapsulation improves the storage, handling, and administration of probiotics by protecting them from environmental factors adverse conditions in gastrointestinal tract. This process facilitates their controlled delivery body, which can simplify use therapies without compromising therapeutic efficacy. Objectives: study investigates microencapsulation Lactiplantibacillus plantarum LM-20, its probiotic properties, effects a murine model ulcerative colitis. Methods/Results: Synbiotic was carried out using spray drying with maltodextrin, gum Arabic, inulin, achieving an encapsulation efficiency 90.76%. The resulting microcapsules exhibited remarkable resistance to simulated vitro, maintaining survival rate 90%. did not compromise characteristics bacteria, as they demonstrated enhanced auto-aggregation, hydrophobicity, phenol tolerance. potential microencapsulated synbiotic evaluated dextran sodium sulfate-induced results revealed that mice treated LM-20 showed 83.3% reduction disease activity index (DAI) compared colitis control group. Moreover, significant decrease observed pro-inflammatory cytokine levels (IL-1β TNF-α) myeloperoxidase activity, values comparable those healthy Conclusions: These findings suggest could be promising candidate for applications prevention management

Language: Английский

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The Networked Interaction between Probiotics and Intestine in Health and Disease: A Promising Success Story

Microorganisms, Journal Year: 2024, Volume and Issue: 12(1), P. 194 - 194

Published: Jan. 18, 2024

Probiotics are known to promote human health either precautionary in healthy individuals or therapeutically patients suffering from certain ailments. Although this knowledge was empirical past tomes, modern science has already verified it and expanded new limits. These microorganisms can be found nature various foods such as dairy products supplements formulated for clinical preventive use. The current review examines the different mechanisms of action probiotic strains how they interact with organism host. Emphasis is put on therapeutic use these beneficial conditions gastrointestinal tract. Diseases tract particularly any malfunction inflammation intestines seriously compromise whole organism. interaction between host’s microbiota alleviate signs symptoms while some cases, due course, intervene underlying pathology. Various safety issues probiotics also discussed.

Language: Английский

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An integrative multi-omic analysis defines gut microbiota, mycobiota, and metabolic fingerprints in ulcerative colitis patients

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 8, 2024

Background Ulcerative colitis (UC) is a multifactorial chronic inflammatory bowel disease (IBD) that affects the large intestine with superficial mucosal inflammation. A dysbiotic gut microbial profile has been associated UC. Our study aimed to characterize UC bacterial, fungal, and metabolic fingerprints by omic approaches. Methods The 16S rRNA- ITS2-based metataxonomics gas chromatography–mass spectrometry/solid phase microextraction (GC–MS/SPME) metabolomic analysis were performed on stool samples of 53 patients 37 healthy subjects (CTRL). Univariate multivariate approaches applied separated integrated data, define microbiota, mycobiota, signatures in interaction between bacteria fungi was investigated network analysis. Results In cohort, we reported increase Streptococcus , Bifidobacterium Enterobacteriaceae, TM7-3, Granulicatella Peptostreptococcus Lactobacillus Veillonella Enterococcus Peptoniphilus Gemellaceae, phenylethyl alcohol; also decrease Akkermansia ; Ruminococcaceae; Ruminococcus Gemmiger Methanobrevibacter Oscillospira Coprococus Christensenellaceae Clavispora Vishniacozyma Quambalaria hexadecane; cyclopentadecane; 5-hepten-2-ol, 6 methyl; 3-carene; caryophyllene; p-Cresol; 2-butenal; indole, 3-methyl-; 6-methyl-3,5-heptadiene-2-one; 5-octadecene; 5-hepten-2-one, methyl. integration multi-omic data confirmed presence distinctive fingerprint microbiota. Moreover, highlighted bacterial fungal synergistic and/or divergent interkingdom interactions. Conclusion this study, identified intestinal UC-associated biomarkers. Furthermore, evidence relationships ecosystems provides comprehensive perspective dysbiosis ecological interactions microorganisms framework

Language: Английский

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Bacteria-based drug delivery for treating non-oncological diseases

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 366, P. 668 - 683

Published: Jan. 18, 2024

Language: Английский

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Advances and optimization strategies in bacteriophage therapy for treating inflammatory bowel disease

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 8, 2024

In the advancement of Inflammatory Bowel Disease (IBD) treatment, existing therapeutic methods exhibit limitations; they do not offer a complete cure for IBD and can trigger adverse side effects. Consequently, exploration novel therapies multifaceted treatment strategies provides patients with broader range options. Within framework IBD, gut microbiota plays pivotal role in disease onset through diverse mechanisms. Bacteriophages, as natural microbial regulators, demonstrate remarkable specificity by accurately identifying eliminating specific pathogens, thus holding promise. Although clinical trials have affirmed safety phage therapy, its efficacy is prone to external influences during storage transport, which may affect infectivity regulatory roles within microbiota. Improving stability precise dosage control bacteriophages—ensuring robustness consistent dosing, targeted delivery infection sites—is crucial. This review thoroughly explores latest developments inherent challenges, focusing on interaction between bacteriophages. It highlights bacteriophages’ potential microbiome modulators offering detailed insights into research bacteriophage encapsulation Particular attention paid functionality various carrier systems, especially regarding their protective properties ability colon-specific delivery. aims provide theoretical foundation using bacteriophages paving way enhanced regulation intestinal

Language: Английский

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Healing from Within: How Gut Microbiota Predicts IBD Treatment Success—A Systematic Review

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8451 - 8451

Published: Aug. 2, 2024

Recent research indicates that the microbiome has a significant impact on progression of inflammatory bowel disease (IBD) and creating therapies change its composition could positively outcomes IBD treatment. This review summarizes results extensive studies examined patients undergoing several therapies, including anti-TNF medication, vedolizumab, ustekinumab, probiotics, fecal microbiota transplantation (FMT), alterations in their gut microbiota's function. The objective was to investigate variety effectiveness microbial species order discover new biomarkers or therapeutic targets improve outcome treatment for these patients. aimed offer useful insights into personalized medicine techniques managing IBD. Beneficial bacteria such as Faecalibacterium prausnitzii Roseburia have been consistently linked favorable clinical outcomes, whereas pathogenic Escherichia coli Clostridioides difficile are associated with worsening conditions. Although many role IBD, there is still need more targeted connection between specific communities outcomes. study sought address this gap by exploring intricate relationship medications.

Language: Английский

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Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 22, 2025

Numerous studies have confirmed that gut microbiota is a key driver in the occurrence and progression of inflammatory bowel disease (IBD). Based on bacterial collection constructed our previous studies, we founded Bifidobacterium adolescentis AF91-08b2A has potential beneficial function. We designed cohort genomic animal experiments to further explore probiotic function its therapeutic effect IBD. The depletion B. individuals with IBD suggested significance for intestinal health. Genomic analysis highlighted attributes AF91-08b2A, including resistance antibiotics stress, metabolic pathways related energy carbohydrate metabolism, which are likely enhance efficacy. In DSS-induced mice colitis model, strain significantly enhanced activity index (DAI), curbed weight loss, attenuated colonic damage. It effectively modulated immune response by reducing levels pro-inflammatory cytokines such as IL-6, IL-1β, IL-17A, IFN-γ, TNF-α, while promoting secretion anti-inflammatory like IL-4, IL-10, TGF-β1. restoration tight junction proteins ZO-1, occludin, claudin-2 demonstrated capacity safeguard epithelial barrier. Collectively, findings indicate valuable option UC, multifaceted approach inflammation fortifying

Language: Английский

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Pediatric kidney transplant recipients are at an increased risk for dysbiosis

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 30, 2025

Introduction This study aimed to compare the gut microbiota composition in pediatric kidney transplant (KTx) recipient with that of healthy children. Methods cross-sectional observational included 30 KTx recipients aged between 7 and 21 years 25 The was assessed using 16S rRNA gene sequencing, alpha beta diversity, as well all statistical analyses, conducted Phyloseq library R programming language. Taxonomic profiles were evaluated QIIME2, differences compared linear discriminant analysis effect size (LEFSe) an LDA threshold >2 p < 0.05. Results No significant found diversity controls. However, exhibited alterations composition, including higher relative abundances Verrucomicrobiota at phylum level, Akkermansia Neisseria genus level ( 0.05 for all). Conversely, there a decrease bacterial genera belonging Firmicutes. In addition, history frequent urinary tract infections, diarrhea reduced GFR showed increases abundance Discussion Pediatric demonstrated alterarions indicating dysbiosis. Further studies are needed elucidate cause-and-effect relationships these changes their impact on clinical consequencies long-term prognosis.

Language: Английский

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Conversion of whey and other dairy waste into antimicrobial and immunoregulatory compounds by fermentation

Frontiers in Food Science and Technology, Journal Year: 2025, Volume and Issue: 5

Published: March 5, 2025

Fermentation by lactic acid bacteria has been used for millennia to preserve food and make it more palatable. These microorganisms may also generate bioactive compounds with the potential serve as components in active edible packaging or alternative therapeutics. Dairy waste products, especially whey, provide a substrate growth of these bacteria, can be incorporated into formulations packaging. This minireview deals use dairy grow produce compounds, specifically antimicrobial peptides immunoregulatory molecules, their therapeutic applications.

Language: Английский

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