IL-17A inhibitors alleviate Psoriasis with concomitant restoration of intestinal/skin microbiota homeostasis and altered microbiota function

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 28, 2024

Disturbed gut microbiota and associated metabolic dysfunction exist in Psoriasis. Despite the growing use of interleukin-17 inhibitor (anti-IL17) therapy, effect anti-IL17 on gut/skin function is not fully understood patients with Therefore, we explored whether Psoriasis alterations selected a study cohort, longitudinal cohort to reveal effects IL-17A treatment In case-control study, 14 10 age, sex body mass index-matched Healthy Controls were recruited. Longitudinal mapping microbiome was performed using 16S rRNA gene sequencing. Mouse models used further validate interrelationship between skin PICRUST2 applied predict bacterial community. patients, dysbiosis present increased heterogeneity: decreased Bacteroidota Firmicutes as well Actinobacteriota predominating Escherichia-Shigella enrichment reduction serum levels total bile acid markers Apoptotic pathways. After studies observed trend toward normal distribution modulation apoptosis-related Results from mouse model showed dysregulation characterized by Staphylococcus colonization. The psoriatic exhibits loss community stability pathogen enrichment. inhibitors restore homeostasis pathways, reduce pro-inflammatory cytokine expression, alleviate symptoms

Language: Английский

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Exploring the mediating role of blood metabolites in the relationship between gut microbiota and gastric cancer risk: a Mendelian randomization study

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 7, 2025

Prior studies have established correlations between gut microbiota (GM) dysbiosis, circulating metabolite alterations, and gastric cancer (GC) risk. However, the causal nature of these associations remains uncertain. We utilized summary data from genome-wide association (GWAS) on GM (European, n=8,956), blood metabolites n=120,241; East Asian, n=4,435), GC n=476,116; n=167,122) to perform a bidirectional Mendelian randomization (MR) analysis, investigating effects Additionally, we conducted mediation analysis (two-step MR) identify potential mediators in GM-GC relationship. identified twelve negative seven positive specific taxa For metabolites, traits were found be significantly associated with reduced risk European population, findings subsequently validated Asian cohort. Three showed five metabolic traits: Bacteroidia class Bacteroidales order positively correlated (all P < 0.013), while Bacteroides OTU97_27 exhibited correlation one (P = 0.007). Two-step MR indicated that total lipids intermediate-density lipoprotein (IDL), IDL particle concentration, phospholipids medium low-density (LDL), small LDL, free cholesterol LDL indirectly influenced class/Bacteroidales GC, proportions 1.71% 0.048), 1.69% 2.05% 0.045), 1.85% 1.99% respectively. The present study provides suggestive evidence relationship GM, risk, potentially offering new insights into etiology.

Language: Английский

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Investigating causal associations between pork intake and multiple sclerosis using Mendelian randomization: Insights from large-scale GWAS data in European populations

Nutrition and Health, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Background Observational studies propose associations between dietary factors and multiple sclerosis (MS). However, the causal nature of these relationships remains unclear. This study aims to determine whether nutritional causally influence MS risk through Mendelian randomization (MR) analysis. Methods Summary statistics for 15 common were derived from Genome-Wide Association Study (GWAS) conducted by Medical Research Council Integrated Epidemiology Unit (MRC-IEU). The GWAS data sourced International Multiple Sclerosis Genetics Consortium (IMSGC), including 14,498 cases 24,091 controls. inverse-variance weighted (IVW) method was utilized as primary analytical approach estimate causality, while Cochran's Q test assessed heterogeneity. MR-Egger (MRE) MR-Pleiotropy RESidual Sum Outlier (MR-PRESSO) employed detect pleiotropy conduct sensitivity analyses. Results We executed univariable- multivariable-adjusted MR analyses, incorporating adjustments past tobacco smoking, particulate matter air pollution (PM2.5) moderate vigorous physical activity. univariable (UVMR) analysis elucidated potential concerning only two factors: pork intake (odds ratio [OR] = 6.764, 95% confidence interval [CI]: 1.772–25.814, p 0.005 IVW) fish/liver oil supplements (OR 0.119, 95%CI: 0.032–0.444, 0.002 IVW). In multivariate (MVMR) analysis, significant relationship remained robust even after adjusting confounders. Conversely, confounders, initially observed ceased be statistically significant. Conclusions Our findings suggest that could increase MS. Other analyzed in this did not exhibit correlations with

Language: Английский

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Multi-Omics Approach to Evaluate Effects of Dietary Sodium Butyrate on Antioxidant Capacity, Immune Function and Intestinal Microbiota Composition in Adult Ragdoll Cats

Metabolites, Journal Year: 2025, Volume and Issue: 15(2), P. 120 - 120

Published: Feb. 11, 2025

Objectives: Sodium butyrate (SB) is a typical postbiotic known to positively affect economic animals in recent years, but research on SB pet cats scarce. Consequently, this study sought explore the influence of anti-inflammatory and antioxidant capacity, immune function, gut microbiota adult through assessment biochemical parameters comprehensive integrative omics analysis. Methods: A total 30 were divided into three groups: basal diet (NC), with 0.05% (SB5), 0.1% (SB10). The experiment lasted for 6 weeks. Results: results indicated that fecal level calprotectin was lower SB10 group than SB5 NC groups. reduced serum levels TNF-α, IL-1β DAO compared (p < 0.05). In addition, increased GSH-Px decreased MDA content Transcriptomic analysis showed gene expression VCAM1 exhibited notable decrease revealed richness higher 0.05), abundance Lachnospiraceae, Lachnoclostridium, Blautia, Roseburia greater Conclusions: Dietary could enhance improve regulate composition cats.

Language: Английский

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Diagnosis of Cholangiocarcinoma: The New Biological and Technological Horizons

Diagnostics, Journal Year: 2025, Volume and Issue: 15(8), P. 1011 - 1011

Published: April 16, 2025

The diagnosis of cholangiocarcinoma (CCA) remains challenging. Although new technologies have been developed and validated, their routine use in clinical practice is needed. Conventional cytology obtained during endoscopic retrograde cholangiopancreatography-guided brushings the first-line technique for CCA, but it has shown limited sensitivity when combined with ultrasound-guided biopsy. Other diagnostic tools proposed respective advantages limitations. Cholangioscopy biopsy or FISH analysis, intraductal biliary ultrasound confocal laser microscopy made significant advances last decade. More recently, developments analytical “omics” sciences allowed mapping microbiota patients liquid proteomic extracellular vesicle analysis identification biomarkers that can be incorporated into predictive diagnostics. Furthermore, preoperative setting, radiomics, radiogenomics integrated artificial intelligence may provide useful foundations personalized therapy hepatobiliary diseases. This review aims to evaluate current approaches innovative translational research CCA.

Language: Английский

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Identifying Proteins and Amino Acids Associated with Liver Cancer Risk: A Study Utilizing Mendelian Randomization and Bulk RNA Sequencing Analysis

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(3), P. 262 - 262

Published: Feb. 28, 2024

Background: Primary liver cancer (PLC) ranks third in terms of fatality rate among all malignant tumors worldwide. Proteomics and metabolomics have become widely utilized identifying causes diagnostic indicators PLC. Nevertheless, studies aiming to identify proteins/metabolites that experienced significant changes before PLC, the potential impact reverse causation confounding variables still needs be fully addressed. Methods: This study thoroughly investigated causal relationship between 4719 blood proteins, 21 amino acids, risk PLC using Mendelian randomization (MR) method. In addition, through a comprehensive analysis TCGA-LIHC cohort GEO databases, we evaluated differentially expressed genes (DEGs) related serine metabolism diagnosing predicting prognosis patients with Results: A total 63 proteins been identified as connected Additionally, there has confirmation positive cause–effect concentration serine. The integration findings from both MR analyses determined protein associated exhibited correlation metabolism. Upon careful cohort, it was found eight DEGs are linked After validating database, two DEGs, TDO2 MICB, emerged biomarkers for Conclusions: Two involved metabolism, MICB TDO2, causally could potentially used indicators.

Language: Английский

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BACTEREMIA CAUSED BY VEILLONELLA PARVULA. TWO CASE REPORTS AND A REVIEW OF THE LITERATURE

Anaerobe, Journal Year: 2024, Volume and Issue: 88, P. 102879 - 102879

Published: June 19, 2024

Language: Английский

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Causal associations of gut microbiota and pulmonary tuberculosis: a two-sample Mendelian randomization study

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 14, 2024

The prevalence of pulmonary tuberculosis (PTB) as an infectious disease continues to contribute significantly global mortality. According recent studies, the gut microbiota PTB patients and healthy controls (HCs) show significant disparities. However, causal relationship between them has yet be elucidated.

Language: Английский

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Decoding the microbiota metabolome in hepatobiliary and pancreatic cancers: Pathways to precision diagnostics and targeted therapeutics

Pharmacological Research, Journal Year: 2024, Volume and Issue: 208, P. 107364 - 107364

Published: Aug. 23, 2024

We delve into the critical role of gut microbiota and its metabolites in pathogenesis progression hepatobiliary pancreatic (HBP) cancers, illuminating an urgent need for breakthroughs diagnostic therapeutic strategies. Given high mortality rates associated with HBP which are attributed to aggressive recurrence, metastasis, poor responses chemotherapy, exploring microbiome research presents a promising frontier. This highlights how microbial metabolites, including secondary bile acids, short-chain fatty lipopolysaccharides, crucially influence cancer cell behaviors such as proliferation, apoptosis, immune evasion, significantly contributing oncogenesis cancers. By integrating latest findings, we discuss association alterations key their implications, metabolomics microbiomics can enhance precision. Furthermore, paper explores strategies targeted therapies through metabolomics, direct effects potential synergistic on conventional therapies. also recognize that field diagnosis treatment tumors still has lot problems be solved. The aim this study is pioneer metabolite provide reference diagnosis, treatment, prognosis.

Language: Английский

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A bidirectional Mendelian randomisation study of the association between gut microbiota and malignant non-melanoma skin cancer

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 23, 2024

Gut microbiota are suggested to be associated with a variety of cancers.However, the correlation between gut and development malignant non-melanoma skin cancer (MNMSC) remains unknown. Thus, this study employed bidirectional Mendelian randomisation (MR) method investigate potentially causal association MNMSC. Here detailed genetic data on available from MiBioGen consortium Dutch Microbiota Project were analysed, as well MNMSC UK Biobank.Several sophisticated statistical methods involving inverse variance weighting, weighted median estimator, MR Egger, simple mode-based estimator models utilised assess potential Reverse sensitivity analyses also performed. Eight identified causally related Genera Holdemanella (odds ratio [OR]=0.99, 95% confidence interval [CI]: 0.99–1.00, 4.5×10-3), Ruminococcaceae UCG014 (OR=0.99, CI: P=1.3×10-2), Sutterella P=4.2×10-2), Bifidobacterium adolescentis P=8.8×10-3), Bacteroides dorei (OR=1.00, P=9.9×10-3), family Veillonellaceae 0.99–1.00,P=3.8×10-4) negatively correlated Clostridia (OR=1.01, 1.00–1.02, P=2.1×10-3) Clostridiales positively The was more pronounced following Bonferroni correction. No relationship determined any these eight by reverse analysis. This suggests that specific have effects indicates certain may effect modulation means prevent

Language: Английский

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