Role of immunocytochemistry in cervical cancer screening DOI Open Access
Vinod B. Shidham

CytoJournal, Journal Year: 2022, Volume and Issue: 19, P. 42 - 42

Published: June 14, 2022

The cervical cancer screening has been based conventionally on cytologic analysis. With advances in understanding the role of human papillomavirus, cotesting applied. But most patients subjected to colposcopy did not benefit, except cases with HSIL [high-grade squamous intraepithelial lesion] cytology. Because this, a step increase sensitivity detect cancers and pre-cancers but higher specificity minimal overdiagnosis leading prevention unindicated biopsies is highly desired. Such triaging abnormal results expected minimize invasive interventions because low false positivity. availability methodologies quantitatively qualitatively enhanced cell-blocks from residual liquid cytology specimens, immunostaining can be performed for multiple immunomarkers potential objectivity triage initial test results. This further inclusion AV marker application SCIP (subtractive coordinate immunoreactivity pattern) approach. are also resource performing other ancillary studies including molecular pathology proteomics/metabolomics as tests future. review explores specimen cell-blocking algorithmic manner adjunct ASCCP management guidelines improved patient care.

Language: Английский

Human Papillomavirus Epidemiology and Prevention: Is There Still a Gender Gap? DOI Creative Commons
Giovanna Milano, Giovanni Guarducci, Nicola Nante

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(6), P. 1060 - 1060

Published: June 4, 2023

Background and aim: Human papillomavirus (HPV) is sexually transmitted, one of the three most common transmitted infections (STIs) in both males females, viral STI. A crucial public health strategy to protect people against HPV vaccination, which has shown its effectiveness preventing HPV-related diseases. Presently, types vaccines are available (bivalent, quadrivalent, nonvalent), they all target two oncogenic virus genotypes (HPV 16 18). In recent years, need implement vaccination programmes that include genders been discussed order achieve herd immunity HPV. To date, only a few countries have included young their programmes. Thus, our objective with this review provide an overview epidemiology prevention strategies report latest findings from scientific literature.

Language: Английский

Citations

30
MicroRNA dysregulations in Merkel cell carcinoma: Molecular mechanisms and clinical applications DOI
Chiara Mazziotta,

Christian Felice Cervellera,

Carmen Lanzillotti

et al.

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)

Published: Dec. 7, 2022

Merkel cell carcinoma (MCC) is an aggressive skin malignancy with two distinct etiologies. The first, which accounts for the highest proportion, caused by polyomavirus (MCPyV), a DNA tumor virus. A second, UV-induced, MCC form has also been identified. Few diagnostic, prognostic, and therapeutic options are available. MicroRNAs (miRNAs) small noncoding RNA molecules, play key role in regulating various physiologic cellular functions including cycling, proliferation, differentiation, apoptosis. Numerous miRNAs dysregulated cancer, acting as either suppressors or oncomiRs. aim of this review to collect, summarize, discuss recent findings on whose dysregulation assumed MCC. potential clinical application diagnostic prognostic biomarkers described. In future, will potentially gain significance improvement options.

Language: Английский

Citations

33
Integration of scRNA-Seq and TCGA RNA-Seq to Analyze the Heterogeneity of HPV+ and HPV- Cervical Cancer Immune Cells and Establish Molecular Risk Models DOI Creative Commons

Erdong Wei,

Amin Reisinger,

Jiahua Li

et al.

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: June 1, 2022

Numerous studies support that Human papillomavirus (HPV) can cause cervical cancer. However, few have surveyed the heterogeneity of HPV infected or uninfected (HPV+ and HPV-) cancer (CESC) patients. Integration scRNA-seq TCGA data to analyze HPV+ HPV- patients on a single-cell level could improve understanding cellular mechanisms during HPV-induced cancer.CESC obtained from Gene Expression Omnibus (GEO) database Seurat, Monocle3 package were used for analysis. The ESTIMATE was single-sample gene immune score, CIBERSORT identify scores cells, "WGCNA" weighted correlation network Univariate Cox LASSO regression performed establish survival relapse signatures. KEGG GO analyses signature gene. Profiling Interactive Analysis Pan-cancer analysis.In CESC group, CD8+ T cells B down-regulated, whereas reg CD4+ epithelial up-regulated according data. Survival analysis TCGA-CESC revealed increased expression naive favors probability WGCNA, univariate Cox, established 9-genes 7-gene model. identified IKZF3, FOXP3, JAK3 had similar distribution effects in HPV-associated HNSC.Analysis bulk RNA-seq samples transcriptional state infiltration. models adjusted assess prognostic risk accurately. Hub genes represent protection associated HNSC while showing irrelevant other potential HPV-related cancers.

Language: Английский

Citations

25
Tumor associated macrophage in HPV+ tumors: Between immunosuppression and inflammation DOI Creative Commons
Laurent Gorvel, Daniel Olive

Seminars in Immunology, Journal Year: 2022, Volume and Issue: 65, P. 101671 - 101671

Published: Nov. 29, 2022

Language: Английский

Citations

22
Human Papillomavirus Epidemiology and Prevention: Is There Still a Gender Gap? DOI Open Access
Giovanna Milano, Giovanni Guarducci, Nicola Nante

et al.

Published: April 19, 2023

Background and aim: Human Papillomavirus (HPV) is sexually transmitted, one of the three most common transmitted infections (STIs) in both males females, viral STI. A crucial public health strategy to protect people against HPV through vaccination, which has shown its effectiveness preventing related diseases. Presently types vaccines are available (bivalent, quadrivalent, nonavalent) they all target at least two oncogenic virus genotypes (HPV 16, 18). In past few years, need implement vaccination programmes that include genders been discussed order achieve herd immunity HPV. To date, only a countries have included young their programmes, so we aim this review provide an overview epidemiology prevention strategies prevent it, reporting latest findings from scientific literature.

Language: Английский

Citations

11
Expression of miRNAs in the Presence of HPV Infection in Cervical Dysplasia Samples: A Pilot Study DOI Creative Commons

A.V. Svintsitska,

N.F. Lygyrda,

V. S. Svintsitskyi

et al.

Experimental Oncology, Journal Year: 2025, Volume and Issue: 46(4), P. 387 - 392

Published: Feb. 20, 2025

Background. Cervical cancer is a major health concern, with human papillomaviruses (HPV) infection being key risk factor. However, not all HPV-infected individuals develop cancer, suggesting the additional factors may be involved. This study aims to evaluate differences in miR-155 and -205 expression cervical tissue dysplasia depending on presence of HPV confirmed diagnosis. Materials Methods. The 30 formalin-fixed paraffin-embedded primary biopsy samples was evaluated using RT-PCR. Results. levels miRNA-155 without malignant transformation lower than these carcinoma situ tissues (0.74 ± 0.21 1.65 0.42 vs. 1.37 0.18 2.35 0.32, respectively). In cases, we found higher (1.6 1.38 times, respectively) CIN-3/ HSIL compared CIN-2/HSIL samples. both miRNAs tended increase HPV-positive cases HPV-negative signs transformation, respectively. Conclusions. obtained data indicate potential relationship between profile -205.

Language: Английский

Citations

0
Role of infectious agents in Cancer pathogenesis and therapy DOI Creative Commons

Ezichi F. Ofoezie,

C Ogbonna,

Sandra C. Olisakwe

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100284 - 100284

Published: March 1, 2025

Language: Английский

Citations

0
Role of Epigenetics in Cervical Cancer Radiotherapy Treatment Outcomes DOI
Kamalesh Dattaram Mumbrekar, S P Srinivas Nayak,

Karthika Balaji

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Role of miRNAs in Cervical Cancer DOI
Sachin Shetty, Ayush Khandelwal,

Roopal Behera

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Bibliometric Analysis of Research on cervical cancer and miRNAs from 2010 to 2024: Research Trends, HotSpots, and Prospects DOI

Cong Xu,

Yonghong Xu,

Guangming Wang

et al.

Published: April 28, 2025

Abstract Background and Purpose MicroRNAs (miRNAs) play multifaceted roles in cervical cancer therapy, including regulating progression, metastasis, drug resistance, HPV control, metabolic alterations. This study aims to provide a comprehensive bibliometric analysis of the existing literature on miRNAs cancer, offering insights into research trends, key contributors, emerging themes guide future investigations enhance therapeutic strategies. Method We conducted systematic search Web Science PubMed database for published between January 2010 December 2024. A total 4034 records were retrieved analyzed using VOSviewer CiteSpace software visualization trend analysis. Results Over past fifteen years, miRNA showed significant upward before 2020, then gradually declined starting from 2021. The reveals that Tang Hua are most active authors, China is influential country. "Plos One" journal publishes articles. Besides, Tianjin Medical University productive institution. top three high-frequency keywords "cervical cancer", "expression" "invasion". Recent keyword indicates notable feature current deep integration basic clinical application. Particularly, cross-integration non-coding RNA network with technologies such as nanotechnology artificial intelligence promoting precise diagnosis treatment system cancer. These findings highlight interest understanding miRNA-mediated pathways their significance Conclusion provides overview landscape identifying institutions, trends. While does not predict direction treatment, it offers valuable state potential areas further exploration. underscore importance continued investigation mechanisms applications advance management.

Language: Английский

Citations

0