Role of immunocytochemistry in cervical cancer screening DOI Open Access
Vinod B. Shidham

CytoJournal, Journal Year: 2022, Volume and Issue: 19, P. 42 - 42

Published: June 14, 2022

The cervical cancer screening has been based conventionally on cytologic analysis. With advances in understanding the role of human papillomavirus, cotesting applied. But most patients subjected to colposcopy did not benefit, except cases with HSIL [high-grade squamous intraepithelial lesion] cytology. Because this, a step increase sensitivity detect cancers and pre-cancers but higher specificity minimal overdiagnosis leading prevention unindicated biopsies is highly desired. Such triaging abnormal results expected minimize invasive interventions because low false positivity. availability methodologies quantitatively qualitatively enhanced cell-blocks from residual liquid cytology specimens, immunostaining can be performed for multiple immunomarkers potential objectivity triage initial test results. This further inclusion AV marker application SCIP (subtractive coordinate immunoreactivity pattern) approach. are also resource performing other ancillary studies including molecular pathology proteomics/metabolomics as tests future. review explores specimen cell-blocking algorithmic manner adjunct ASCCP management guidelines improved patient care.

Language: Английский

miRNAs that regulate apoptosis in breast cancer and cervical cancer DOI
Elmira Aboutalebi Vand Beilankouhi,

Maral Salek Maghsoodi,

Maryam Zamani Sani

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(3), P. 1993 - 2006

Published: July 6, 2024

Language: Английский

Citations

3
Impact of Human Papillomavirus on microRNA-21 Expression in Oral and Oropharyngeal Cancer—A Systematic Review DOI Open Access

Mario Kordić,

Dinko Martinović,

Ema Puizina

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8038 - 8038

Published: July 23, 2024

Recently, microRNAs (miR) were identified to have potential links with oral squamous cell carcinoma (OSCC) and oropharyngeal (OPSCC) oncogenesis, specifically miR-21. Since HPV is a major risk factor for the development of these diseases, we aimed search literature regarding miR-21 expression in both HPV-positive HPV-negative OSCC/OPSCC. The was performed PubMed (MEDLINE), Scopus, Web Science, Cochrane electronic databases. research question as follows: Is there difference tissue between patients those OSCC/OPSCC? After conducting meticulous strategy, four studies included, they had pooled sample size 621 subjects OSCC and/or OPSCC. Three did not find any significant findings this systematic review showed that are no differences Nevertheless, it worth noting still insufficient important subject, because understanding how influences its downstream effects can provide insights into molecular mechanisms underlying OSCC/OPSCC progression.

Language: Английский

Citations

3
miRNA in Molecular Diagnostics DOI Creative Commons
Maja Matulić, Paula Gršković, Andreja Petrović

et al.

Bioengineering, Journal Year: 2022, Volume and Issue: 9(9), P. 459 - 459

Published: Sept. 9, 2022

MicroRNAs are a class of small non-coding RNA molecules that regulate gene expression on post-transcriptional level. Their biogenesis consists complex series sequential processes, and they many genes involved in all cellular processes. function is essential for maintaining the homeostasis single cell; therefore, their aberrant contributes to development progression diseases, especially malignant tumors viral infections. Moreover, can be associated with certain states specific disease, obtained least invasive manner patients analyzed basic molecular methods used clinical laboratories. Because this, have promising potential become very useful biomarkers tools personalized medicine approaches. In this review, miRNAs biogenesis, significance cancer infectious current available test detection summarized.

Language: Английский

Citations

14
Future directions in cervical cancer treatment DOI
Botle Precious Damane, Thanyani Victor Mulaudzi,

Mahlatse Cordelia Kgokolo

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 155 - 177

Published: Jan. 1, 2024

Language: Английский

Citations

2
The role of microRNA-9 in Ovarian and Cervical Cancers: an Updated Overview DOI
Riccardo Di Fiore,

Rosa Drago‐Ferrante,

Sherif Suleiman

et al.

European Journal of Surgical Oncology, Journal Year: 2024, Volume and Issue: unknown, P. 108546 - 108546

Published: July 1, 2024

Language: Английский

Citations

2
Identification of proteins’ expression pathway and the effective miRNAs for the treatment of human papillomavirus-induced cervical cancer: in-silico analyses-experimental research DOI Open Access
Marzieh Monemi, Lida Garrosi,

Samira Mirzaei

et al.

Annals of Medicine and Surgery, Journal Year: 2024, Volume and Issue: 86(10), P. 5784 - 5792

Published: Aug. 30, 2024

Cervical cancer is the fourth most common in women. The risk factors for cervical include human papillomavirus (HPV) infection, age, smoking, number of pregnancies, use oral contraceptives, and diet. However, long-term HPV infection appears to be main factor developing cancer. This in-silico analysis aims identify expression network proteins miRNAs that play a role development HPV-induced

Language: Английский

Citations

2
The Role of microRNA Expression and DNA Methylation in HPV-Related Cervical Cancer: A Systematic Review DOI Open Access
Alessandra Pulliero,

Giulia Cassatella,

Pietro Astuni

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12714 - 12714

Published: Nov. 26, 2024

Human papillomavirus (HPV) infection is a major etiologic factor in cervical cancer, cause of cancer-related morbidity and mortality among women worldwide. The role microRNA (miRNA) dysregulation carcinogenesis still largely unknown, but epigenetic changes, including DNA methylation miRNA regulation, are crucial factors. integration HPV into the host genome can lead to alterations patterns expression, contributing progression from normal epithelium intraepithelial neoplasia and, ultimately, cancer. This review aimed examine relationship between changes development associated with A systematic literature search was conducted databases using predefined inclusion exclusion criteria. Studies that investigated function, clinical significance miRNAs, methylation, expression oncoproteins HPV-related cancer were included. Data extraction, quality assessment, synthesis performed provide comprehensive overview current state knowledge. We an studies investigating relation progression, highlighting their common outcomes weaknesses/strengths. To achieve this, we systematically searched Pubmed database for all articles published January 2018 December 2023. Our revealed substantial body evidence supporting pivotal pathogenesis related oncoproteins. From 28 retrieved, miR-124, FAM194/miR-124-2, most frequently down- or up-regulated CC progression. Notably, FAM194/miR-124-2 emerged as promising molecular marker distinguishing cases requiring immediate surgical intervention those amenable more conservative wait-and-see approach. underscores critical involvement context sheds light on potential utility discriminatory tool guiding treatment decisions. identification patients who may benefit early versus suitable observation has important implications personalized targeted management strategies era precision medicine.

Language: Английский

Citations

2
Bioinformatics and cheminformatics approaches to identify pathways, molecular mechanisms and drug substances related to genetic basis of cervical cancer DOI
K. M. Salim Andalib, Md Habibur Rahman, Ahsan Habib

et al.

Journal of Biomolecular Structure and Dynamics, Journal Year: 2023, Volume and Issue: 41(23), P. 14232 - 14247

Published: Feb. 28, 2023

Cervical cancer (CC) is a global threat to women and our knowledge frighteningly little about its underlying genomic contributors. Our research aimed understand the molecular genetic mechanisms of CC by integrating bioinformatics network-based study. Transcriptomic analyses three microarray datasets identified 218 common differentially expressed genes (DEGs) within control samples specimens. KEGG pathway analysis revealed pathways in cell cycle, drug metabolism, DNA replication significant GO terms were cornification, proteolysis, division replication. Protein–protein interaction (PPI) network 20 hub survival validated CDC45, MCM2, PCNA TOP2A as biomarkers. Subsequently, 10 transcriptional factors (TFs) post-transcriptional regulators detected through TFs-DEGs miRNAs-DEGs regulatory assessment. Finally, biomarkers subjected drug-gene relationship find best target inhibitors. Standard cheminformatics method including silico ADMET docking study substantiated PD0325901 Selumetinib most potent candidate-drug for treatment. Overall, this meticulous holds promises further vitro vivo on diagnosis, prognosis therapies.Communicated Ramaswamy H. Sarma

Language: Английский

Citations

6
The mechanisms and diagnostic potential of lncRNAs, miRNAs, and their related signaling pathways in cervical cancer DOI Creative Commons
Yan Xu, Yu Sun,

Song Xiao-bin

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: May 4, 2023

Cervical cancer (CC), the fourth most prevalent type of among women worldwide, is associated with high rates morbidity and mortality. Due to long period latency in CC, patients are already middle late stages when initially diagnosed, which greatly reduces clinical cure rate quality survival, thus resulting poor outcomes. In recent years, continuous exploration fields bioinformatics molecules, it has been found that ncRNAs, including miRNAs lncRNAs, without ability translate proteins capable activating or inhibiting certain signaling pathways by targeting modulating level expression involved these pathways. ncRNAs play important roles assisting diagnosis, drug administration, prediction prognosis during CC progression. As an entry point, mechanisms interaction between miRNAs, have a focus basic research relating numerous experimental studies confirmed close relationship development. Against this background, we summarize latest advances involvement lncRNA- miRNA-related development provide guidance for treatment.

Language: Английский

Citations

6
Hsa-miR-194-5p and hsa-miR-195-5p are down-regulated expressed in high dysplasia HPV-positive Pap smear samples compared to normal cytology HPV-positive Pap smear samples DOI Creative Commons
Ali Dehghani,

Fardin Khajepour,

Mohammad Amin Dehghani

et al.

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 12, 2024

The human papillomavirus (HPV) infection may affect the miRNA expression pattern during cervical cancer (CC) development. To demonstrate association between high-risk HPVs and development of cervix dysplasia, we examined patterns hsa-miR-194-5p hsa-miR-195-5p in Pap smear samples from southeast Iranian women. We compared that were HPV-positive but showed no abnormality cytological examination to had severe dysplasia.

Language: Английский

Citations

1