Ascorbic Acid Enhances the Inhibitory Effect of Theasaponins against Candida albicans DOI Open Access
Yuhong Chen, Ying Gao,

Junfeng Yin

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10661 - 10661

Published: Oct. 3, 2024

Candida albicans (C. albicans) is a main cause of hospital-acquired fungal infections. Combination therapy promising as novel anti-C. strategy because its better efficacy. Theasaponins are pentacyclic triterpenes in the Camellia genus with multiple biological activities. Our previous studies prove that theasaponins display inhibitory activity against C. albicans. Ascorbic acid (VC) vitamin found many plants shows potential combination therapy. However, whether VC enhances remains unclear. In this study, checkerboard micro-dilution method was used to assess effect (0–80 mmol/L) on (0–1000 μg/mL). Then, effects (31.25 μg/mL), (80 mmol/L), and μg/mL) + planktonic cells different stages biofilm formation were assessed. Transcriptomic analysis conducted investigate molecular mechanisms. According results, enhanced anti-planktonic anti-biofilm The minimum concentration significantly decreased fungicidal efficiency increased addition VC. remarkably aggravated suppression regard various virulence factors albicans, including adhesion, early formation, mature biofilm, cell surface hydrophobicity, phospholipase activity. Compared or groups, level intracellular reactive oxygen species higher, while levels mitochondrial membrane adenosine triphosphate lower group, suggesting more severe oxidative stress, injury, energy deficiency. revealed predominantly suppressed pathways glycolysis, glycerophospholipid metabolism, glutathione cysteine methionine metabolism. This implied deficiency redox imbalance associated combination. These results has be topical antifungal disinfectant.

Language: Английский

A Preclinical Model for Parkinson’s Disease Based on Transcriptional Gene Activation via KEAP1/NRF2 to Develop New Antioxidant Therapies DOI Creative Commons
Juan Segura‐Aguilar, Bengt Mannervik

Antioxidants, Journal Year: 2023, Volume and Issue: 12(3), P. 673 - 673

Published: March 9, 2023

Investigations of the effect antioxidants on idiopathic Parkinson's disease have been unsuccessful because preclinical models used to propose these clinical studies do not accurately represent neurodegenerative process disease. Treatment with certain exogenous neurotoxins induces massive and extremely rapid degeneration; for example, MPTP causes severe Parkinsonism in just three days, while degenerative Parkinson´s proceeds over many years. The endogenous neurotoxin aminochrome seems be a good alternative target since it is formed nigrostriatal system neurons where occurs. Aminochrome all mechanisms reported involved processes presence neuromelanin-containing dopaminergic postmortem brain healthy elderly people suggests that neuromelanin synthesis normal harmless despite fact requires oxidation dopamine ortho-quinones are potentially toxic, especially aminochrome. apparent contradiction harmless, its formation via neurotoxic ortho-quinones, can explained by protective roles DT-diaphorase glutathione transferase GSTM2-2 as well neuroprotective role astrocytes secreting exosomes loaded GSTM2-2. Increasing expression may therapeutic goal prevent degeneration new neurons. Several phytochemicals induce discovered and, therefore, an interesting question whether phytochemical KEAP1/NRF2 activators inhibit or decrease aminochrome-induced neurotoxicity.

Language: Английский

Citations

18
The complex interplay between ferroptosis and atherosclerosis DOI Open Access

Mao Zhang,

Jianping Li, Wei Hu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117183 - 117183

Published: July 30, 2024

Atherosclerosis, characterized by the accumulation of plaque within arterial walls, is an intricate cardiovascular disease that often results in severe health issues. Recent studies have emphasized importance ferroptosis, a controlled type cell death dependent on iron, as critical factor this state. Ferroptosis, distinguished its reliance iron and lipid hydroperoxides, offers unique insight into pathology atherosclerotic lesions. This summary encapsulates current knowledge role ferroptosis plays onset progression atherosclerosis. It explores molecular processes through which peroxidation metabolism contribute to development atheromatous plaques evaluates possibility utilizing novel treatment approach for By illuminating relationship between ferroptosis-related atherosclerosis, review paves way future clinical applications personalized medicine approaches aimed at alleviating effects

Language: Английский

Citations

5
Potential Role of Glutathione Antioxidant Pathways in the Pathophysiology and Adjunct Treatment of Psychiatric Disorders DOI Creative Commons
Nicole Poladian,

Inesa Navasardyan,

William Narinyan

et al.

Clinics and Practice, Journal Year: 2023, Volume and Issue: 13(4), P. 768 - 779

Published: July 5, 2023

Oxidative stress is defined as the imbalance between production of free radicals and their removal by antioxidants, leading to accumulation subsequent organ tissue damage. Antioxidant status its role in has been observed a number psychological disorders. Glutathione commonly referred principal antioxidant brain and, therefore, plays critical maintaining redox homeostasis. Reduced levels glutathione increase vulnerability oxidative stress, may be associated with development progression several psychiatric Within this review, we focus on analyzing potential associations pathway disorders: major depressive disorder, schizophrenia, bipolar generalized anxiety disorder. Our research suggests that studies regarding these four disorders have shown decreased GSH association diseased states; however, conflicting results note no significant variance enzymes and/or metabolites based state. In studying NAC administration an adjunct therapy, various augment therapy aid symptomatic management for disorders, while contrasting exist within literature. Based findings throughout there room study further suggest need such pathways consideration interactions first-line pharmacotherapy, use antioxidants supplemental therapy.

Language: Английский

Citations

12
Temporal colonization and metabolic regulation of the gut microbiome in neonatal oxen at single nucleotide resolution DOI Creative Commons
Quanbin Dong,

Dongxu Hua,

Xiuchao Wang

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

The colonization of microbes in the gut is key to establishing a healthy host-microbiome symbiosis for newborns. We longitudinally profiled microbiome model consisting 36 neonatal oxen from birth up 2 months postpartum and carried out microbial transplantation reshape their microbiome. Genomic reconstruction deeply sequenced fecal samples resulted total 3931 metagenomic-assembled genomes 472 representative species, which 184 were identified as new species when compared with existing databases oxen. Single nucleotide level metagenomic profiling shows rapid influx after birth, followed by dynamic shifts during first few weeks life. Microbial was found genetic makeup 33 (FDR < 0.05), mainly Prevotella Bacteroides species. further linked over 20 million single variations 736 plasma metabolites, enabled us characterize 24 study-wide significant associations (P 4.4 × 10-9) that identify potential regulation host immune neuro-related including glutathione L-dopa. Our integration analyses revealed may influence health status growth performance modulating metabolites via structural encoded proteins. For instance, we albumin levels antioxidant capacity correlated L-dopa, determined regulations metabolic enzymes. current results indicate temporal transplantation-driven strain replacement are crucial newborn development, offering insights enhancing growth.

Language: Английский

Citations

4
Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging DOI Creative Commons
Estefanía Díaz‐Del Cerro, Irene Martínez de Toda, Judith Félix

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(8), P. 1529 - 1529

Published: July 30, 2023

The oxidative-inflammatory theory of aging states that is the result establishment a chronic stress situation in which immune system implicated. Among redox parameters, those involved glutathione cycle have been suggested as essential aging. Thus, first objective this study was to determine if several components (glutathione reductase (GR) and peroxidase (GPx) activities, concentrations oxidized (GSSG) reduced (GSH)) leukocytes) are associated with biological age (ImmunolAge) estimated using Immunity Clock 190 men women. second identify best blood fraction (whole blood, cells, erythrocytes, or plasma) quantify these correlate them ImmunolAge. results show oxidative state peripheral leukocytes correlates their functionality, supporting idea basis immunosenescence. In correlations more significant cells respect ImmunolAge (positive GSSG concentration GSSG/GSH ratio, negative GPx GR activities). Therefore, proposed most effective sample estimate individuals clinical settings.

Language: Английский

Citations

10
Association of sulfur mustard toxicity with oxidant/antioxidant system in veterans: A meta-analysis of case-control studies DOI
Hossein Hassanpour,

Marzieh Mojtahed,

Leila Nasiri

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 114007 - 114007

Published: Jan. 9, 2025

Language: Английский

Citations

0
Gliadin-Rich Diet Worsens Immune and Redox Impairments in Prematurely Aging Mice DOI Creative Commons
Estefanía Díaz‐Del Cerro, António Garrido, Julia Cruces

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(4), P. 279 - 279

Published: Feb. 14, 2025

Gliadin is one of the most important fractions gluten, a glycoprotein closely linked to development negative effects on physiological functions and gastrointestinal diseases, such as celiac disease (CD). Research suggests that inadequate stress responses anxiety states may trigger or at least contribute these pathological conditions. Peritoneal leukocytes from Prematurely Aging Mice (PAM), which are chronologically adult mice with compromised anxiety, exhibit functional changes when exposed in vitro gliadin peptides, resembling some immune alterations found also CD patients. This observation prompted us investigate gliadin-rich diet function redox state PAM. In this study, female PAM were fed either gluten-enriched (PAMD, 120 g/kg) standard (PAMC) for four weeks. Immune parameters peritoneal, splenic, thymic (phagocytosis, chemotaxis, Natural Killer activity, lymphoproliferation) markers (glutathione reductase, glutathione peroxidase, reduced/oxidized glutathione, xanthine oxidase lipid peroxidation) evaluated. The results showed PAMD exhibited more impaired function, lower antioxidant enzyme activities, reduced concentrations, well higher oxidized increased activity compared PAMC. These findings suggest worsens impairments PAM, previously described CD, indicating potential animal studying gluten-induced dysregulation.

Language: Английский

Citations

0
Polystyrene nanoplastics exacerbate gentamicin-induced nephrotoxicity in adult rat by activating oxidative stress, inflammation and apoptosis pathways DOI

Khadija Boukholda,

Awatef Elwej,

Sabrine Ben Slimen

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Language: Английский

Citations

0
Unlocking the Potential of l-α-Glycerylphosphorylcholine: From Metabolic Pathways to Therapeutic Applications DOI
Xiaohang Che, Yang Zhao,

Zhongtian Xu

et al.

Nutrition Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

l-α-Glycerylphosphorylcholine (GPC), also known as choline alphoscerate or α-glycerophosphorylcholine, serves both a pharmaceutical product and dietary supplement. Through its metabolic pathways, GPC acts the precursor not only of acetylcholine but various phospholipids. Extensive preclinical clinical evidence demonstrates that effectively alleviates cognitive impairment associated with Alzheimer's disease, vascular dementia, cerebral ischemia, stress, epilepsy, among other conditions. Additionally, has beneficial effects on such conditions measures ischemic/hypoxic conditions, ionizing radiation-induced damage, exercise performance, growth hormone release, liver damage. As well facilitating cholinergic neurotransmission, indicates GPC, activities, can promote γ-aminobutyric acid enhance protein kinase C activity, facilitate hippocampal neurogenesis, upregulate neurotrophic factors, inhibit inflammation. In studies, results indicate is genotoxic in vitro vivo. human studies causes no severe adverse effects. Possible risks atherosclerosis stroke await necessary validation. this review, GPC-related pharmacological effects, mechanisms action, safety evaluation are discussed aim providing comprehensive understanding GPC.

Language: Английский

Citations

0
Peroxidase-like Active Cu-MOFs Nanozymes for Colorimetric Detection of Total Antioxidant Capacity in Fruits and Vegetables DOI Creative Commons
Yanyan Huang,

Jiatong Han,

Ping Yi

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(8), P. 1311 - 1311

Published: April 9, 2025

In this study, two types of Cu-MOFs (Cu-TCPP and CuO-TCPP) with a two-dimensional layered porous structure were prepared via in situ polymerization using Cu2+, CuO, TCPP as raw materials. Both exhibited peroxidase-like activity, capable catalyzing the oxidation TMB by H2O2 to form oxTMB, resulting an absorption peak at 652 nm color change from colorless blue. Subsequently, addition AA can reduce oxTMB back TMB, causing system lighten or become colorless. Based on principle, simple rapid colorimetric method for detection was established successfully applied TAC fruits vegetables. The results showed that Cu-TCPP CuO-TCPP had large linear range ascorbic acid 0.01–100 mM (Cu-TCPP) 0.05–100 (CuO-TCPP). This study not only provides novel preparing nanozymes but also offers approach analyzing food.

Language: Английский

Citations

0