The role of long noncoding RNA MEG3 in fibrosis diseases DOI
Wenlong Wu,

Sijing Zhou,

Guanghe Fei

et al.

Postgraduate Medical Journal, Journal Year: 2023, Volume and Issue: 100(1186), P. 529 - 538

Published: Dec. 8, 2023

Fibrosis is a prevalent pathological condition observed in various organs and tissues. It primarily arises from the excessive abnormal accumulation of extracellular matrix, resulting structural functional impairment tissues organs, which can culminate death. Many forms fibrosis, including liver, cardiac, pulmonary, renal are considered irreversible. Maternally expressed gene 3 (MEG3) an imprinted RNA gene. Historically, downregulation MEG3 has been linked to tumor pathogenesis. However, recent studies indicate emerging association with fibrotic diseases. In this review, we delve into current understanding MEG3's role aiming shed light on molecular mechanisms fibrosis potential as novel therapeutic target.

Language: Английский

LncRNADisease v3.0: an updated database of long non-coding RNA-associated diseases DOI Creative Commons
Lin Xiao,

Yingyu Lu,

Chenhao Zhang

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 52(D1), P. D1365 - D1369

Published: Oct. 11, 2023

Abstract Systematic integration of lncRNA-disease associations is great importance for further understanding their underlying molecular mechanisms and exploring lncRNA-based biomarkers therapeutics. The database long non-coding RNA-associated diseases (LncRNADisease) designed the above purpose. Here, an updated version (LncRNADisease v3.0) has curated comprehensive lncRNA (including circRNA) disease from burgeoning literatures. LncRNADisease v3.0 exhibits over 2-fold increase in experimentally supported associations, with a total 25440 entries, compared to last version. Besides, each pair assigned confidence score based on experimental evidence. Moreover, all between lncRNAs/circRNAs are classified into general causal representing whether lncRNAs or circRNAs can directly lead development progression corresponding diseases, 15721 9719 respectively. In case study, we used data calculate phenotypic similarity human mouse lncRNAs. This will continue serve as valuable resource potential clinical applications related circRNAs. freely available at http://www.rnanut.net/lncrnadisease.

Language: Английский

Citations

43
BMSC‑derived exosome‑mediated miR‑25‑3p delivery protects against myocardial ischemia/reperfusion injury by constraining M1‑like macrophage polarization DOI Creative Commons
Jingxia Du, Yibo Dong, Jingjing Song

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 30(2)

Published: June 18, 2024

Myocardial ischemia/reperfusion injury (MIRI) is a significant challenge in the management of myocardial ischemic disease. Extensive evidence suggests that macrophage‑mediated inflammatory response may play vital role MIRI. Mesenchymal stem cells and, particular, exosomes derived from these cells, be key mediators and repair. However, whether protect heart by regulating polarization macrophages exact mechanisms involved are poorly understood. The present study aimed to determine secreted bone marrow mesenchymal (BMSC‑Exo) harboring miR‑25‑3p can alter phenotype affecting JAK2/STAT3 signaling pathway, which reduces protects against An in vivo MIRI model was established rats ligating anterior descending region left coronary artery for 30 min followed reperfusion 120 min, BMSC‑Exo carrying (BMSC‑Exo‑25‑3p) were administered through tail vein injection. A hypoxia‑reoxygenation H9C2 established, cocultured with BMSC‑Exo‑25‑3p vitro. results demonstrated or could taken up cardiomyocytes powerful cardioprotective effects decreasing cardiac infarct size, reducing incidence malignant arrhythmias attenuating enzyme activity, as indicated lactate dehydrogenase creatine kinase levels. It induced M1‑like macrophage after (I/R), evidenced increase iNOS expression immunofluorescence staining upregulation proinflammatory cytokines RT‑qPCR, such interleukin‑1β (IL‑1β) interleukin‑6 (IL‑6). As hypothesized, inhibited cytokine while promoting M2‑like polarization. Mechanistically, pathway activated I/R LPS‑stimulated vitro, pretreatment this activation. indicate attenuation related inactivation subsequent inhibition

Language: Английский

Citations

10
Role of gut microbiota in thalassemia: a review of therapeutic prospects DOI Creative Commons
Guanjun Chen, Yulan Li,

S. H. Wei

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

In recent years, the study of gut microbiota has gradually become a research hotspot in field medicine, as dysbiosis is closely related to various diseases. Thalassemia, hereditary hemoglobinopathy, complex pathophysiological mechanism, and traditional treatment methods show limited efficacy. With deeper understanding microbiome, researchers have begun focus on its role pathogenesis thalassemia therapeutic effects. This article aims review potential prospects, analyze latest findings, explore impact mechanisms patients with thalassemia, goal providing new ideas directions for future clinical thalassemia.

Language: Английский

Citations

0
ncRNA-Mediated Pyroptosis in Cerebral Ischemia-Reperfusion Injury: Pathophysiology, Mechanisms, and Therapeutic Perspectives DOI Creative Commons

Ruiyi Xu,

Peng Quan, Wen Chen

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 141 - 141

Published: Feb. 22, 2025

Cerebral ischemia-reperfusion injury (CIRI) is a complex pathological process triggered by transient obstruction of blood flow and subsequent reperfusion, ultimately leading to intracellular disturbances such as oxidative stress, inflammatory responses, programmed cell death. Among the various types death, pyroptosis (an kind regulated death) has received increasing attention due its involvement in key neurovascular unit cells, including endothelial neurons, microglia, astrocytes. Intriguingly, accumulating evidence demonstrates that non-coding RNAs (ncRNAs), long RNAs, microRNAs, circular can modulate multiple stages CIRI. This review synthesizes recent findings on ncRNAs-regulated We highlight molecular underpinnings pyroptotic activation following ischemic discuss how ncRNAs shape these mechanisms. By elucidating interactions between pyroptosis-related pathways, we intend present innovative viewpoints for early diagnosis development potential therapeutic strategies mitigate

Language: Английский

Citations

0
Harnessing the Role of ESR1 in Breast Cancer: Correlation with microRNA, lncRNA, and Methylation DOI Open Access
Shengping Yang, Claudio Manna, Pulak R. Manna

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3101 - 3101

Published: March 27, 2025

Breast cancer (BC) is a multifactorial condition and it primarily expresses the estrogen receptor α (ERα) that encoded by gene 1 (ESR1), which modulates signaling. ESR1, facilitating overproduction, plays an indispensable role in progression survival of majority BCs. To obtain molecular insights into these phenomena, we analyzed The Cancer Genome Atlas (TCGA) breast invasive carcinoma (BRCA) RNA-Seq datasets for expression ESR1 its correlation to microRNAs (miRNAs) long non-coding RNAs (lncRNAs), along with methylation patterns. Regulation was also assessed total 43 cancerous non-cancerous cell lines. Analyses both TCGA BRCA line data revealed specific lncRNAs, i.e., MEG3, BIK, MLL, FAS are negatively correlated PARP1 demonstrates positive association. Additionally, miR-30a miR-145 showed negative correlations expression. Of 54 loci analyzed, them exhibited expression, highlighting potentially modifiable regulatory mechanism. These findings underscore complex events influencing interaction diverse signaling pathways, demonstrating novel pathogenesis potential therapeutics.

Language: Английский

Citations

0
The Regulatory Role of NcRNAs in Pyroptosis and Disease Pathogenesis DOI

Shaocong Wang,

Xinzhe Chen, Kun Wang

et al.

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Language: Английский

Citations

0
Salvianolic acids and its potential for cardio-protection against myocardial ischemic reperfusion injury in diabetes DOI Creative Commons
Yuxin Jiang, Yin Cai,

Ronghui Han

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 12, 2024

The incidence of diabetes and related mortality rate increase yearly in modern cities. Additionally, elevated glucose levels can result an reactive oxygen species (ROS), ferroptosis, the disruption protective pathways heart. These factors collectively heighten vulnerability diabetic individuals to myocardial ischemia. Reperfusion therapies have been effectively used clinical practice. There are limitations current methods treat ischemia-reperfusion injury. As a result, reducing post-treatment ischemia/reperfusion injury remains challenge. Therefore, efforts underway provide more efficient therapy. Salvia miltiorrhiza Bunge (Danshen) has for centuries ancient China cardiovascular diseases (CVD) with rare side effects. Salvianolic acid is water-soluble phenolic compound potent antioxidant properties greatest hydrophilic property Danshen. It recently discovered that salvianolic acids A (SAA) B (SAB) capable inhibiting apoptosis by targeting JNK/Akt pathway NF-κB pathway, respectively. This review delves into most recent discoveries regarding therapeutic cardioprotective benefits diabetes. shows great potential protection mellitus. thorough understanding mechanism could expand its uses developing medicines treating mellitus ischemia-reperfusion.

Language: Английский

Citations

2
MEG3 shuttled by exosomes released from human bone marrow mesenchymal stem cells promotes TP53 stability to regulate MCM5 transcription in keloid fibroblasts DOI

Feibin Zhu,

Yuanjian Ye,

Ying Shao

et al.

The Journal of Gene Medicine, Journal Year: 2024, Volume and Issue: 26(5)

Published: April 30, 2024

Despite the interest in mesenchymal stem cells (MSC), their potential to treat abnormal scarring, especially keloids, is yet be described. The present study aimed investigate therapeutic of exosomes derived from human bone marrow MSCs (hBMSC-Exos) alleviating keloid formation.

Language: Английский

Citations

2
Long Non-Coding RNAs and Proliferative Retinal Diseases DOI Creative Commons
Anamika Sharma, Nikhlesh K. Singh

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(5), P. 1454 - 1454

Published: May 10, 2023

Retinopathy refers to disorders that affect the retina of eye, which are frequently caused by damage retina’s vascular system. This causes leakage, proliferation, or overgrowth blood vessels through retina, can lead retinal detachment breakdown, resulting in vision loss and, rare cases, blindness. In recent years, high-throughput sequencing has significantly hastened discovery new long non-coding RNAs (lncRNAs) and their biological functions. LncRNAs rapidly becoming recognized as critical regulators several key processes. Current breakthroughs bioinformatics have resulted identification lncRNAs may a role disorders. Nevertheless, mechanistic investigations yet reveal relevance these Using lncRNA transcripts for diagnostic and/or therapeutic purposes aid development appropriate treatment regimens long-term benefits patients, traditional medicines antibody therapy only provide temporary must be repeated. contrast, gene-based therapies tailored, solutions. Here, we will discuss how different retinopathies, including age-related macular degeneration (AMD), diabetic retinopathy (DR), central vein occlusion (CRVO), proliferative vitreoretinopathy (PVR), prematurity (ROP), cause visual impairment blindness, retinopathies identified treated using lncRNAs.

Language: Английский

Citations

6
The lncRNA MEG3/miRNA-21/P38MAPK axis inhibits coxsackievirus 3 replication in acute viral myocarditis DOI Creative Commons
Feng He, Zhuo Liu, Feng Miao

et al.

Virus Research, Journal Year: 2023, Volume and Issue: 339, P. 199250 - 199250

Published: Nov. 1, 2023

Evidence is emerging on the roles of long noncoding RNAs (lncRNAs) as regulatory factors in a variety viral infection processes, but mechanisms underlying their functions coxsackievirus group B type3 (CVB3)-induced acute myocarditis have not been explicitly delineated. We previously demonstrated that CVB3 decreases miRNA-21 expression; however, lncRNAs regulate miRNA-21-dependent disease process yet to be identified. To evaluate upstream miRNA-21, differentially expressed CVB3-infected mouse hearts were identified by microarray analysis and lncRNA/miRNA-21 interactions predicted bioinformatically. MEG3 was candidate miRNA-21-interacting lncRNA upregulated hearts. expression verified HeLa cells 48 h post act competitive endogenous RNA miRNA-21. knockdown resulted upregulation which inhibited replication attenuating P38-MAPK signaling vitro vivo. Knockdown before alleviated cardiac injury, improved survival. Furthermore, CREB5, bioinformatically function MEG3, decrease replication. This study identifies MEG3/miRNA-21/P38 MAPK axis replication, for CREB5 could serve an modulator.

Language: Английский

Citations

5