Biological effects and mechanisms of fisetin in cancer: a promising anti-cancer agent

European journal of medical research, Journal Year: 2023, Volume and Issue: 28(1)

Published: Aug. 25, 2023

Abstract Fisetin, a natural flavonoid, possesses numerous biological activities that have been extensively studied in various diseases. When it comes to cancer, fisetin exhibits range of effects, such as suppressing cell growth, triggering programmed death, reducing the formation new blood vessels, protecting against oxidative stress, and inhibiting migration. Moreover, has ability enhance effectiveness chemotherapy. The anticancer properties can be attributed diverse array molecules signaling pathways, including vascular endothelial growth factor (VEGF), mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB), PI3K/Akt/mTOR, Nrf2/HO-1. Consequently, holds promise therapeutic agent for treatment. In this review, we place emphasis on functions molecular targets therapy.

Language: Английский

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Genotoxic and Cytotoxic Activity of Fisetin on Glioblastoma Cells

Anticancer Research, Journal Year: 2024, Volume and Issue: 44(3), P. 901 - 910

Published: Feb. 29, 2024

Background/Aim: Fisetin is a yellow-coloring flavonoid that can be found in wide variety of plants, vegetables, and fruits, such as strawberries, apples, grapes. It has been shown to have biological activity by targeting different pathways regulating survival death bear antioxidant anti-inflammatory activity. was cytotoxic on cancer cell lines the ability kill therapy-induced senescent cells. The aim study investigate DNA damaging potential fisetin its enhance killing effect temozolomide glioblastoma Materials Methods: We used LN229 cells measured apoptosis flow cytometry, strand breaks alkaline comet γH2AX assay, damage response western blot analysis. Results: cells, inducing apoptosis. In dose range 40-80 μM it also induced damage, triggered response, revealed p53 activation. Furthermore, enhanced genotoxic methyl methanesulfonate, presumably due inhibition repair processes. When administered together with temozolomide, first-line therapeutic for glioblastoma, death, reduced yield following treatment exhibited senolytic Conclusion: Data show high-dose suggest that, harnessing flavonoid, may anticancer drugs eliminate Therefore, useful adjuvant therapy, including which worth studied clinical trials.

Language: Английский

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Effects of Fisetin Treatment on Cellular Senescence of Various Tissues and Organs of Old Sheep

Antioxidants, Journal Year: 2023, Volume and Issue: 12(8), P. 1646 - 1646

Published: Aug. 21, 2023

Fisetin has been shown to be beneficial for brain injury and age-related disease via different mechanisms. The purpose of this study was determine the presence senescent cells effects fisetin on cellular senescence in other vital organs old sheep, a more translational model. Female sheep 6–7 years (N = 6) were treated with 100 mg/kg or vehicle alone two consecutive days week 8 weeks. All harvested at time sacrifice. Histology, immunofluorescence staining, RT-Q-PCR performed regions tissues organs. Our results indicated that treatment current regimen did not affect general morphology brain. both cerebral cortex cerebellum non-Cornu Ammonis (CA) area hippocampus detected by senescent-associated β-galactosidase (SA-β-Gal) staining GL13 (lipofuscin) staining. mainly neurons gray white matter either cortex, cerebellum, non-CA hippocampus. Very few CA1-4 hippocampus, as revealed GLB1 colocalization NEUN. significantly decreased number SA-β-Gal+ GL13+ showed decreasing trend cerebellum. Furthermore, P16+ GLB1+ neuronal nuclear protein (NEUN)+ neurons, glial fibrillary acidic (GFAP)+ astrocytes, ionized calcium binding adaptor molecule 1 (IBA1)+ microglia cortex. cells, NEUN+ plasma S100B. At mRNA level, downregulated liver, lung, heart, spleen tissues, P21 expression liver lung. TREM2 lung downregulation spleen, heart. A significant decrease NRLP3 observed after treatment. Finally, SOD1 while upregulating CAT spleen. In conclusion, we found widely present sheep. addition, gene expressions inflammasomes organs, such liver. represents promising therapeutic strategy diseases.

Language: Английский

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Activation of Heme Oxygenase-1 by Mangiferin in Human Retinal Pigment Epithelial Cells Contributes to Blocking Oxidative Damage

Biomolecules & Therapeutics, Journal Year: 2024, Volume and Issue: 32(3), P. 329 - 340

Published: April 8, 2024

Mangiferin is a kind of natural xanthone glycosides and known to have various pharmacological activities. However, since the beneficial efficacy this compound has not been reported in retinal pigment epithelial (RPE) cells, study aimed evaluate whether mangiferin could protect human RPE ARPE-19 cells from oxidative injury mimicked by hydrogen peroxide (H

Language: Английский

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Fisetin alleviates Salmonella typhimurium-induced colitis through the TLR2/TLR4-NF-κB pathway, regulating microbiota, and repressing intracellular bacterial proliferation by focal adhesion kinase

European Journal of Nutrition, Journal Year: 2025, Volume and Issue: 64(3)

Published: March 19, 2025

Language: Английский

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Phloroglucinol Attenuates DNA Damage and Apoptosis Induced by Oxidative Stress in Human Retinal Pigment Epithelium ARPE-19 Cells by Blocking the Production of Mitochondrial ROS

Antioxidants, Journal Year: 2022, Volume and Issue: 11(12), P. 2353 - 2353

Published: Nov. 28, 2022

Phloroglucinol, a phenolic compound, is known to possess potent antioxidant ability. However, its role in retinal cells susceptible oxidative stress has not been well elucidated yet. Thus, the objective of this study was evaluate whether phloroglucinol could protect against damage cultured human pigment epithelium ARPE-19 cells. For purpose, were stimula ted with hydrogen peroxide (H2O2) mimic stress. Cell viability, cytotoxicity, apoptosis, reactive oxygen species (ROS) generation, mitochondrial function, DNA damage, and autophagy then assessed. Our results revealed that ameliorated cell H2O2-exposued blocked production ROS. Phloroglucinol also counteracted H2O2-induced apoptosis by reducing Bax/Bcl-2 ratio, blocking activation caspase-3, inhibiting degradation poly (ADP-ribose) polymerase. H2O2 caused impairment increased expression levels mitophagy markers such as PINK1and PARKIN be associated ROS (mtROS) generation cytosolic release cytochrome c. these changes significantly attenuated phloroglucinol. Mito-TEMPO, selective antioxidant, further enhanced protective effect dysfunctional mitochondria. Furthermore, induced autophagy, but when pretreated phloroglucinol, meaning contributed pro-survival mechanism protected from autophagy. Taken together, suggest can inhibit stress-induced dysfunction protecting subsequent through mitigation mtROS generation. might have therapeutic potential prevent stress-mediated RPE

Language: Английский

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Fisetin Protects C2C12 Mouse Myoblasts from Oxidative Stress-Induced Cytotoxicity through Regulation of the Nrf2/HO-1 Signaling

Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 33(5), P. 591 - 599

Published: Jan. 20, 2023

Language: Английский

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The interplay between mitochondria, the gut microbiome and metabolites and their therapeutic potential in primary mitochondrial disease

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 25, 2024

The various roles of the mitochondria and microbiome in health disease have been thoroughly investigated, though they are often examined independently context chronic disease. However, closely connected, namely, through their evolution, maternal inheritance patterns, overlapping role many diseases importance maintenance human health. concept known as "mitochondria-microbiome crosstalk" is ongoing bidirectional crosstalk between these two entities warrants further exploration consideration, especially primary mitochondrial disease, where dysfunction can be detrimental for clinical manifestation composition rarely investigated. A potential mechanism underlying this metabolites from both microbiome. During digestion, gut microbes modulate compounds found food, which produce with bioactive effects. Similarly, produced substrates that undergo biochemical processes during cellular respiration. This review aims to provide an overview current literature examining mitochondria-microbiome crosstalk, commonly studied serve signaling mediating pathways, impact diet has on As a final point, highlights up-to-date implications its therapeutic tool or target.

Language: Английский

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The protective effects of fisetin in metabolic disorders: a focus on oxidative stress and associated events

Journal of Diabetes & Metabolic Disorders, Journal Year: 2024, Volume and Issue: 23(2), P. 1753 - 1771

Published: Oct. 21, 2024

Language: Английский

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Effects of Fisetin Treatment on Cellular Senescence of the Brain and Other Multiple Organs of Old Sheep

Published: June 27, 2023

Fisetin has been shown to be beneficial for brain injury and age-related disease via different mechanisms. The purpose of this study was determine the presence senescent cells effects fisetin on cellular senescence in other organs old sheep, a more translational model. Approximately 6-7 years female sheep (N=6) were treated with 100mg/kg or vehicle two consecutive days week 8 weeks. All harvested at time sacrifice. Histology, immunofluorescent staining, as well Q-PCR performed regions tissues organs. Our results indicated that treatment current regimen did not affect general morphology brain. both cerebral cortex cerebellum detected by SA-β-Gal staining. More observed gray matter when compared white cortex. These are mainly neurons either cerebellum. showed trend decrease significantly decreased matter. Furthermore, P16+ NEUN+ neurons, GFAP+ astrocytes, IBA+ microglia also astrocytes non- (Cornu Ammonis) CA area hippocampus. However, change CA1-4 At mRNA level, GLB1 heart ventricle muscle tissue spleen but tested. antioxidant gene SOD1 increased CAT bone marrow. variable SASP inflammasome genes In conclusion, we found widely present sheep. addition, non-CA represents promising therapeutic strategy disease.

Language: Английский

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