Beyond antibiotics: mesenchymal stem cells and bacteriophages-new approaches to combat bacterial resistance in wound infections

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 52(1)

Published: Dec. 19, 2024

Language: Английский

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Chitosan nanoparticles of new chromone-based sulfonamide derivatives as effective anti-microbial matrix for wound healing acceleration

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 272, P. 132631 - 132631

Published: May 27, 2024

Language: Английский

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Therapeutic potential of mesenchymal stem cells for fungal infections: mechanisms, applications, and challenges

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 30, 2025

As a particularly serious condition in immunocompromised patients, fungal infections (FIs) have increasingly become public health problem worldwide. Mesenchymal stem cells (MSCs), characterized by multilineage differentiation potential and immunomodulatory properties, are considered an emerging strategy for the treatment of FIs. In this study, therapeutic MSCs FIs was reviewed, including their roles played secreting antimicrobial peptides, regulating immune responses, promoting tissue repair. Meanwhile, status research on controversies were also discussed. However, application still faces numerous challenges, such as heterogeneity cell sources, long-term safety, feasibility large-scale production. By analyzing latest study results, review intends to offer theoretical support FI further research.

Language: Английский

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Preclinical study of engineering MSCs promoting diabetic wound healing and other inflammatory diseases through M2 polarization

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 5, 2025

Diabetic foot ulcer (DFU) represents a common and severe complication of diabetes mellitus. Effective safe treatments need to be developed. Mesenchymal stem cells (MSCs) have demonstrated crucial roles in tissue regeneration, wound repair inflammation regulation. However, the function is limited. The safety efficacy gene-modified MSCs unknown. Therefore, this study aimed investigate whether genetically modified with highly efficient expression anti-inflammatory factors promote diabetic by regulating macrophage phenotype transition. This may provide new approach treating healing. In study, human umbilical cord-derived (hUMSCs) were using recombinant lentiviral vectors simultaneously overexpress three factors, interleukin (IL)-4, IL-10, IL-13 (MSCs-3IL). Cell counting kit-8, flow cytometry differentiation assay used detect criteria MSCs. Overexpression efficiency was evaluated cytometry, quantitative real-time PCR, Western blot, enzyme-linked immunosorbent assay, cell scratch assay. We also assessed MSCs-3IL's ability modulate Raw264.7 PCR. addition, we healing through rate calculation, HE staining, Masson immunohistochemical analysis PCNA, F4/80, CD31, CD86, CD206, IL-4, IL-10 IL-13. MSCs-3IL effect on several other models inflammation. efficiently expressed high levels IL-4 (mRNA transcription increased 15,000-fold 800,000-fold, protein secretion 400 200 ng/mL), 950,000-fold, 6 ng/mL). effectively induced phenotypic polarization pro-inflammatory M1-like macrophages (M1) towards M2-like (M2). enhancement does not change phenotype. dynamic distribution vivo normal no karyotype variation tumor risk observed. mouse model, promoted closure exceeding 96% after 14 days treatment. process aided altering (reduced CD86 CD206 expression) accelerating re-epithelialization. summary, our demonstrates that hUMSCs overexpressed key (IL-4, IL-13). MSCs-3IL-based therapy enhances safety. suggests could as novel therapeutic for DFU repair.

Language: Английский

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Application of Gelatin/Vanillin/Fe³⁺/AGP–AgNPs Hydrogels Promotes Wound Contraction, Enhances Dermal Growth Factor Expression, and Minimizes Skin Irritation

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

This study further investigates the potential of gelatin-based hydrogel cross-linked with vanillin and ferric ion (GVF), combined andrographolide (AGP) silver nanoparticles (AgNPs), as an anti-infection biomaterial for wound dressing, aimed at exploring mechanisms that attenuate inflammation, enhance healing rates, address allergic skin irritation. AGP-AgNPs were evaluated cytotoxicity in human adult epidermal keratinocytes (HEKa) murine macrophage cell line (RAW 264.7), well nitric oxide (NO) production response to lipopolysaccharide-induced inflammation RAW 264.7 cells. Skin-wound specimens from male Wistar rats histologically analyzed thickness inflammatory changes. The mRNA expression profiling dermal growth factors was assessed using RT-qPCR, irritation tests conducted female New Zealand rabbits. These exhibited significantly lower toxicity HEKa no 264.7. Interestingly, specific concentrations produced NO control cells but more effective reducing levels pretreated lipopolysaccharides, suggesting AGP-AgNP composites are safe effectively diminish inflammation. Furthermore, a marked increase reduction histological sites observed treated AGP-AgNPs/GVF hydrogels over 21 days. Upregulation genes promoting healing, including collagen types I III, factor, transforming factor-beta, fibronectin, vascular endothelial not fibroblast time-dependent manner. results suggest anti-inflammatory properties GVF/AGP-AgNP could promote epithelialization, cellular proliferation, support extracellular matrix synthesis, facilitate angiogenesis. Additionally, rabbit contact consistently displayed reduced erythema edema, swelling, standardized scoring system yielded low primary indices this hydrogel. findings novel possess anti-inflammatory-like activity can modulate healing. leads irritation, making formulation potentially suitable topical applications care. However, comprehensive studies clinical trials should be required future.

Language: Английский

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Wound healing and anti-inflammatory effects of LAA, the N-acetyl-d-galactosamine-binding lectin from seeds of Luetzelburgia auriculata (Allemão) ducke

Histochemistry and Cell Biology, Journal Year: 2025, Volume and Issue: 163(1)

Published: March 8, 2025

Language: Английский

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The Role of Keratinocytes in Skin Health and Disease

Dermatological Reviews, Journal Year: 2025, Volume and Issue: 6(2)

Published: March 24, 2025

ABSTRACT Aim This study aims to provide a comprehensive analysis of keratinocytes, the predominant cell type in epidermis, by examining their structural, functional, and regulatory roles skin biology. The explores intricate processes keratinocyte differentiation, proliferation, immune interactions, emphasizing essential contributions homeostasis, wound healing, barrier integrity. Additionally, it investigates involvement keratinocytes dermatological disorders such as psoriasis, atopic dermatitis, cancer, highlighting pathological alterations molecular mechanisms. Methods review covers broad range studies, including vitro vivo research on biology, interactions with growth factors, inflammatory responses skin. It also discusses therapeutic advancements biologics gene therapy that focus restoring function diseases. Results highlights play pivotal role inflammation regulation factors promote healing regeneration. Dysregulation is linked several diseases, need for targeted approaches. Recent treatments, biologics, offer promising outcomes managing conditions driven dysfunction. Conclusion primary aim this an in‐depth overview functions particularly health, inflammation, disease development. highlight latest strategies targeting treatment

Language: Английский

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Dexketoprofen-Loaded Alginate-Grafted Poly(N-vinylcaprolactam)-Based Hydrogel for Wound Healing

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3051 - 3051

Published: March 26, 2025

All acute and chronic wound management strategies have limitations. Therefore, there is an urgent need to develop new treatment options for healing. Hydrogels based on natural polymers offer advantages in because they can reduce patients' pain, fight infection, carry targeted drugs speed up the healing process. In this study, we aimed investigate alginate-grafted N-vinylcaprolactam-based matrix a modified release of dexketoprofen (DEX), which potentially useful Free radical polymerization grafted techniques were used prepare thermo-responsive hydrogels. The obtained hydrogels, unloaded hydrogel (HY) dexketoprofen-loaded (DEXHY), characterized analyzed. concentration DEX encapsulated polymer was 4 mg/mL. IC50 values found samples tested by us 607.4 µg/mL HY, 950.4 DEXHY, 2239 DEX. average value cell viability (%) after exposure cells DEXHY 75.4%. exhibited very good vitro closure rate, given its ability modify kinetics. developed study has shown considerable potential facilitate even accelerate healing, including surgical wounds, inhibiting overexpressed inflammation

Language: Английский

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Skin Telocytes Could Fundament the Cellular Mechanisms of Wound Healing in Platelet-Rich Plasma Administration

Cells, Journal Year: 2024, Volume and Issue: 13(16), P. 1321 - 1321

Published: Aug. 8, 2024

For more than 40 years, autologous platelet concentrates have been used in clinical medicine. Since the first formula used, namely platelet-rich plasma (PRP), other experimented with, including fibrin and concentrated growth factor. Platelet three standard characteristics: they act as scaffolds, serve a source of factors cytokines, contain live cells. PRP has become extensively regenerative medicine for successful treatment variety (non-)dermatological conditions like alopecies, acne scars, skin burns, ulcers, muscle, cartilage, bone repair, an adjuvant post-surgery wound healing, with obvious benefits terms functionality aesthetic recovery affected tissues/organs. These indications were well documented, large amount evidence already published supporting efficacy this method. The primordial principle behind minimally invasive treatments is usage patient’s own platelets. transplantation thrombocytes are significant, representing fast economic method that requires only basic equipment training, it biocompatible, thus being low risk patient (infection immunological reactions can be virtually disregarded). Usually, structural applying attributed to fibroblasts only, considered most numerous cell population within interstitium. However, apparent simplistic explanation still eluding those different types interstitial cells (distinct from fibroblasts) residing stromal tissue, e.g., telocytes (TCs). Moreover, dermal TCs documented potential angiogenesis (extra-cutaneous, but also skin), their implication few dermatological was attested described ultrastructurally immunophenotypically. Interestingly, biochemically consists series factors, molecules, which proven positive expression. Thus, attractive hypothesize document any tissular collaboration between cutaneous administered local recovery/repair/regeneration. Therefore, could perceived missing link necessary provide solid good results achieved by administering skin-repairing processes.

Language: Английский

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F127-SE-tLAP thermosensitive hydrogel alleviates bleomycin-induced skin fibrosis via TGF-β/Smad pathway

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: April 19, 2024

Abstract Background Skin fibrosis affects the normal function of skin. TGF-β1 is a key cytokine that organ fibrosis. The latency-associated peptide (LAP) essential for activation. We previously constructed and prepared truncated LAP (tLAP), confirmed tLAP inhibited liver by affecting TGF-β1. SPACE has both transdermal transmembrane functions. promotes delivery macromolecules through stratum corneum into dermis. This study aimed to alleviate skin SPACE. Methods SPACE-tLAP (SE-tLAP) recombinant plasmid was constructed. SE-tLAP purified nickel affinity chromatography. effects on proliferation, migration, expression fibrosis-related inflammatory factors were evaluated in TGF-β1-induced NIH-3T3 cells. F127-SE-tLAP hydrogel using F127 as carrier load polypeptide. degradation, drug release, biocompatibility evaluated. Bleomycin used induce mice. HE, Masson, immunohistochemistry observe histological characteristics. Results significantly reduced ECM production, collagen deposition, fibrotic pathological changes, thereby alleviating Conclusion could increase LAP, reduce production deposition ECM, inhibit formation dermal fibers, progression It may provide new idea therapy

Language: Английский

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