Plant Virus-Derived Vectors for Plant Genome Engineering DOI Creative Commons
Muhammad Arslan Mahmood, Rubab Zahra Naqvi,

Saleem Ur Rahman

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(2), P. 531 - 531

Published: Feb. 14, 2023

Advances in genome engineering (GE) tools based on sequence-specific programmable nucleases have revolutionized precise editing plants. However, only the traditional approaches are used to deliver these GE reagents, which mostly rely Agrobacterium-mediated transformation or particle bombardment. These techniques been successfully for past decades genetic of plants with some limitations relating lengthy time-taking protocols and transgenes integration-related regulatory concerns. Nevertheless, era climate change, we require certain faster developing climate-smart resilient crops through deal global food security. Therefore, alternative needed robustly reagents. In this case, plant viral vectors could be an excellent option delivery reagents because they efficient, effective, precise. Additionally, autonomously replicating considered as natural specialists transient delivery. present review, discussed potential use efficient We further described different vectors, such DNA RNA viruses, gene targeting systems model plants, other important including potato, tomato, wheat, rice. The achievements gained so far a carrier reagent depicted along benefits each vector. Moreover, recent advances explored employing adapting technology future research.

Language: Английский

Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing DOI Creative Commons
Song Zhang, Jiangtao Shen, Dali Li

et al.

Theranostics, Journal Year: 2020, Volume and Issue: 11(2), P. 614 - 648

Published: Oct. 28, 2020

CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy been hindered by efficient delivery materials target cells. Direct system as a ribonucleoprotein (RNP) complex consisting Cas9 protein and single guide RNA (sgRNA) emerged powerful widespread method for due to its advantages transient reduced off-target effects. In review, we summarized current RNP systems including physical approaches synthetic carriers. The mechanisms beneficial roles these strategies intracellular were reviewed. Examples development stimuli-responsive targeted carriers are highlighted. Finally, challenges perspectives rational design next generation promising field will be discussed.

Language: Английский

Citations

311
The future of CRISPR technologies in agriculture DOI
Caixia Gao

Nature Reviews Molecular Cell Biology, Journal Year: 2018, Volume and Issue: 19(5), P. 275 - 276

Published: Jan. 31, 2018

Language: Английский

Citations

247
Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses DOI Creative Commons
Kumar Nishant Chourasia, Milan Kumar Lal, Rahul Kumar Tiwari

et al.

Life, Journal Year: 2021, Volume and Issue: 11(6), P. 545 - 545

Published: June 10, 2021

Among abiotic stresses, salinity is a major global threat to agriculture, causing severe damage crop production and productivity. Potato (Solanum tuberosum) regarded as future food by FAO ensure security, which severely affected salinity. The growth of the potato plant inhibited under salt stress due osmotic stress-induced ion toxicity. Salinity-mediated leads physiological changes in plant, including nutrient imbalance, impairment detoxifying reactive oxygen species (ROS), membrane damage, reduced photosynthetic activities. Several biochemical phenomena, such maintenance water status, transpiration, respiration, use efficiency, hormonal balance, leaf area, germination, antioxidants are adversely affected. ROS increased plasma permeability extravasations substances, causes imbalance plasmolysis. However, plants cope with mediated oxidative conditions enhancing both enzymatic non-enzymatic antioxidant osmoprotectants, proline, polyols (sorbitol, mannitol, xylitol, lactitol, maltitol), quaternary ammonium compound (glycine betaine) synthesized overcome adverse effect response tolerance include complex multifaceted mechanisms that controlled multiple proteins their interactions. This review aims redraw attention researchers explore current physiological, molecular responses subsequently develop potential mitigation strategies against potatoes.

Language: Английский

Citations

148
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement DOI Open Access
Chao Li, Eleanor J. Brant, Hikmet Budak

et al.

Journal of Zhejiang University SCIENCE B, Journal Year: 2021, Volume and Issue: 22(4), P. 253 - 284

Published: April 1, 2021

Language: Английский

Citations

134
CRISPR ribonucleoprotein-mediated genetic engineering in plants DOI Creative Commons
Yingxiao Zhang, Brian J. Iaffaldano, Yiping Qi

et al.

Plant Communications, Journal Year: 2021, Volume and Issue: 2(2), P. 100168 - 100168

Published: Feb. 10, 2021

CRISPR-derived biotechnologies have revolutionized the genetic engineering field and been widely applied in basic plant research crop improvement. Commonly used

Language: Английский

Citations

120
Prevention of Enzymatic Browning by Natural Extracts and Genome-Editing: A Review on Recent Progress DOI Creative Commons
Norfadilah Hamdan, Chia Hau Lee, Syie Luing Wong

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(3), P. 1101 - 1101

Published: Feb. 7, 2022

Fresh fruits and vegetable products are easily perishable during postharvest handling due to enzymatic browning reactions. This phenomenon has contributed a significant loss of food quality appearance. Thus, safe effective alternative method from natural sources is needed tackle prevention. The capabilities anti-browning agents derived plant- animal-based resources in inhibiting activity have been demonstrated the literature. Some also possess strong antioxidants properties. review aims summarize recent investigation regarding use extracts different for controlling browning. potential applications genome-editing preventing improving discussed. Moreover, patents on extract presented this review. information reviewed here could provide new insights, contributing development techniques prevention

Language: Английский

Citations

79
Enzymatic browning and polyphenol oxidase control strategies DOI Creative Commons
Xu Sui, Zan Meng, Tiantian Dong

et al.

Current Opinion in Biotechnology, Journal Year: 2023, Volume and Issue: 81, P. 102921 - 102921

Published: March 23, 2023

Significant amounts of fresh and fresh-cut fruits vegetables are wasted every year due to enzymatic browning. Polyphenol oxidase (PPO) is the key enzyme involved in In past decades, various methods have been developed inhibit browning produce items. However, for most horticultural produce, ideal measures accepted by industries consumers still scarce. This review provides up-to-date knowledge control technologies, including physical methods, chemical such as natural inhibitors, molecular biotechnology, nanotechnology. addition, we propose some ideas improve efficacies these strategies with fewer side effects. To better tissue browning, new research directions also discussed, example, regulation PPO substrate techniques.

Language: Английский

Citations

75
Simultaneous CRISPR/Cas9 Editing of Three PPO Genes Reduces Fruit Flesh Browning in Solanum melongena L. DOI Creative Commons
Alex Maioli, Silvia Gianoglio, Andrea Moglia

et al.

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: Dec. 3, 2020

Polyphenol oxidases (PPOs) catalyze the oxidization of polyphenols, which in turn causes browning eggplant berry flesh after cutting. This has a negative impact on fruit quality for both industrial transformation and fresh consumption. Ten PPO genes (named SmelPPO1 - 10 ) were identified thanks to recent availability high-quality genome sequence. A CRISPR/Cas9-based mutagenesis approach was applied knock-out three target ( SmelPPO4, SmelPPO5 , SmelPPO6) showed high transcript levels An optimized protocol cotyledons used obtain plants Cas9 is directed conserved region shared by genes. The successful editing SmelPPO6 loci vitro regenerated plantlets confirmed Illumina deep sequencing amplicons sites. Besides, potential off-target silico proved absence detectable non-specific mutations. induced mutations stably inherited T 1 2 progeny associated with reduced activity Our results provide first example use CRISPR/Cas9 system biotechnological applications open way development genotypes low maintain polyphenol content berries.

Language: Английский

Citations

101
Can gene editing reduce postharvest waste and loss of fruit, vegetables, and ornamentals? DOI Creative Commons

Emma N. Shipman,

Jingwei Yu, Jiaqi Zhou

et al.

Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)

Published: Jan. 1, 2021

Abstract Postharvest waste and loss of horticultural crops exacerbates the agricultural problems facing humankind will continue to do so in next decade. Fruits vegetables provide us with a vast spectrum healthful nutrients, along ornamentals, enrich our lives wide array pleasant sensory experiences. These commodities are, however, highly perishable. Approximately 33% produce that is harvested never consumed since these products naturally have short shelf-life, which leads postharvest waste. This loss, could be reduced by breeding new retain desirable traits accrue less damage over course long supply chains. New gene-editing tools promise rapid inexpensive production varieties enhanced more easily than was previously possible. Our aim this review critically evaluate gene editing as tool modify biological pathways determine fruit, vegetable, ornamental quality, especially after storage. We brief accessible overviews both CRISPR–Cas9 method chain. Next, we survey literature last 30 years, catalog genes control or regulate quality senescence are “ripe” for editing. Finally, discuss barriers implementing postharvest, from limitations experimental methods international policy. conclude spite hurdles remain, ornamentals likely measurable impact on reducing 5–10 years.

Language: Английский

Citations

92
Advances in Genome Editing With CRISPR Systems and Transformation Technologies for Plant DNA Manipulation DOI Creative Commons
Satya Swathi Nadakuduti, Felix E. Enciso-Rodríguez

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 11

Published: Jan. 14, 2021

The year 2020 marks a decade since the first gene-edited plants were generated using homing endonucleases and zinc finger nucleases. advent of CRISPR/Cas9 for gene-editing in 2012 was major science breakthrough that revolutionized both basic applied research various organisms including consequently honored with “The Nobel Prize Chemistry, 2020.” CRISPR technology is rapidly evolving field multiple CRISPR-Cas derived reagents collectively offer wide range applications beyond. While most these technological advances are successfully adopted to advance functional genomics development innovative crops, others await optimization. One biggest bottlenecks plant has been delivery reagents, genetic transformation methods only established limited number species. Recently, alternative delivering being explored. This review mainly focuses on recent (1) current Cas effectors variants target range, reduced size increased specificity along tissue specific genome editing tool kit (2) cytosine, adenine, glycosylase base editors can precisely install all possible transition transversion mutations sites (3) prime directly copy desired edit into DNA by search replace method (4) mechanisms bypass culture regeneration procedures de novo meristem induction, viral vectors prospects nanotechnology-based approaches.

Language: Английский

Citations

78