Transcriptomic Profiling of Heat-Treated Oriental Lily Reveals LhERF109 as a Positive Regulator of Anthocyanin Accumulation DOI Creative Commons

Mei Zhou,

Lijia Zeng,

Fan Li

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(5), P. 1071 - 1071

Published: April 28, 2025

Pink-flowered Oriental lily cultivars exhibit significant color fading under high temperatures, but the underlying regulatory mechanisms remain unclear. We subjected ‘Souvenir’ plants to temperature treatments (20 °C and 35 °C) performed transcriptome sequencing weighted gene co-expression network analysis (WGCNA). The (35 significantly reduced anthocyanin content in tepals. identified 8354 differentially expressed genes, with GO KEGG analyses revealing a dynamic transition from early stress responses metabolic adaptation. WGCNA revealed module strongly correlated content, which we constructed using known anthocyanin-related including key transcription factor LhMYB12 structural genes involved biosynthetic pathway (LhANS, LhDFR, LhUGT78, LhF3′H). Through this comprehensive analysis, successfully screened LhERF109 as promising candidate. transient overexpression of was found enhance accumulation upregulate LhMYB12, while silencing expression produced opposite effects. These findings identify positive regulator biosynthesis providing new targets for breeding heat-tolerant lilies stable flower coloration.

Language: Английский

Multifaceted roles of LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Lilium spp.) DOI Creative Commons
Mengmeng Bi, Liang Rui, Jiawen Wang

et al.

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(9)

Published: Aug. 22, 2023

The Asiatic hybrid lily (Lilium spp.) is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns. However, the regulatory mechanism underlying flower color has been largely unexplored. Here, we identified WRKY transcription factor from tepals, LhWRKY44, whose expression was closely associated accumulation. Functional verification indicated that LhWRKY44 positively regulated physically interacted LhMYBSPLATTER directly bound to promoter, which enhanced effect of LhMYBSPLATTER-LhbHLH2 MBW complex activator on Moreover, EMSA dual-luciferase assays revealed activated promoters gene LhF3H intracellular anthocyanin-related glutathione S-transferase LhGST. Interestingly, our further results showed participated in light drought-induced accumulation, improved drought tolerance via activating stress-related genes. These generated multifaceted for LhWRKY44-meditaed enhancement by environmental signal pathway accumulation expanded understanding WRKY-mediated transcriptional hierarchy modulating lilies.

Language: Английский

Citations

23
Applications of Virus-Induced Gene Silencing in Cotton DOI Creative Commons
Yue Tian, Fang Yao, Kaixin Zhang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 272 - 272

Published: Jan. 17, 2024

Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become effective tool to investigate function in plants. Cotton one of the most important economic crops globally. In past decade, VIGS been successfully applied cotton functional genomic studies, including those examining abiotic and biotic stress responses vegetative reproductive development. This article summarizes traditional vectors used system, visible markers for endogenous silencing, applications genomics, limitations how they can be addressed cotton.

Language: Английский

Citations

8
Heat-Induced Modulation of Flavonoid Biosynthesis via a LhMYBC2-Mediated Regulatory Network in Oriental Hybrid Lily DOI
Jie Yang, Cong Guo, Feng Chen

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 214, P. 108966 - 108966

Published: July 24, 2024

Language: Английский

Citations

7
For a Colorful Life: Recent Advances in Anthocyanin Biosynthesis during Leaf Senescence DOI Creative Commons

Ziqi Pei,

Huang Yifei, Junbei Ni

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(5), P. 329 - 329

Published: May 9, 2024

Leaf senescence is the last stage of leaf development, and it accompanied by a color change. In some species, anthocyanins are accumulated during senescence, which vital indicators for both ornamental commercial value. Therefore, essential to understand molecular mechanism anthocyanin accumulation would provide new insight into autumn coloration breeding more colorful plants. Anthocyanin surprisingly complex process, significant advances have been made in past decades. this review, we focused on senescence. We emphatically discussed several networks linked genetic, hormonal, environmental, nutritional factors regulating This paper aims regulatory model put forward prospects future development.

Language: Английский

Citations

6
Development and application of CRISPR/Cas9 to improve anthocyanin pigmentation in plants: Opportunities and perspectives DOI
Enerand Mackon, Guibeline Charlie Jeazet Dongho Epse Mackon, Yongqiang Guo

et al.

Plant Science, Journal Year: 2023, Volume and Issue: 333, P. 111746 - 111746

Published: May 23, 2023

Language: Английский

Citations

13
LhANS-rr1, LhDFR, and LhMYB114 Regulate Anthocyanin Biosynthesis in Flower Buds of Lilium ‘Siberia’ DOI Open Access
Shaozhong Fang, Mi Lin, Muhammad Moaaz Ali

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(3), P. 559 - 559

Published: Feb. 23, 2023

The bulb formation of Lilium is affected by many physiological and biochemical phenomena, including flower bud differentiation, starch sucrose accumulation, photoperiod, carbon fixation, plant hormone transduction, etc. transcriptome analysis buds hybrid ‘Siberia’ at different maturity stages showed that floral associated with the accumulation anthocyanins. results HPLC-MS cyanidin major anthocyanin found in ‘Siberia’. Transcriptome KEGG enrichment qRT-PCR validation two genes related to flavonoid biosynthesis (LhANS-rr1 LhDFR) were significantly up-regulated. functional differential revealed LhMYB114 was directly among 19 MYB transcription factors. Furthermore, suggested their expression patterns very similar developmental lily bulbs. Virus-induced gene silencing (VIGS) down-regulation LhANS-rr1, LhDFR, could lead a decrease turning purple phenotype into white color. Moreover, this first report reveal can regulate mature stage anthocyanins an important sign maturity. Therefore, these findings have laid solid theoretical foundation for further discussion on development future.

Language: Английский

Citations

12
Multi-omics analysis uncovers novel gene regulatory networks of flower coloration in Lagerstroemia indica DOI
Fenni Lv,

Rutong Yang,

Qing Wang

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 216, P. 118710 - 118710

Published: May 14, 2024

Language: Английский

Citations

4
LvbHLH13 Regulates Anthocyanin Biosynthesis by Activating the LvMYB5 Promoter in Lily (Lilium ‘Viviana’) DOI Creative Commons

Wenzhong An,

Yibo Sun,

Zhenhua Gao

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(9), P. 926 - 926

Published: Aug. 30, 2024

Anthocyanins, constituents of flavonoid compounds prevalent in plants, possess significant value both plant development and human nutrition. The regulation anthocyanin biosynthesis primarily involves the orchestration MYB, bHLH, WD40 transcription factors. Consequently, bHLH family assumes a pivotal role modulating developmental processes. In present investigation, factor, denoted as LvbHLH13, was identified positive regulator pigmentation lily petals. LvbHLH13 is classified within IIId subgroup Arabidopsis proteins. Functional analyses involving transient expression gene silencing revealed its capacity to enhance diminish accumulation, respectively, by LvMYB5 expression, thereby influencing downstream structural expression. overexpression resulted an increase genes related synthesis, whereas correspondingly decreased Yeast one-hybrid EMSA assays demonstrated interaction between promoter, leading activation biosynthesis. A further luciferase (LUC) analysis corroborated stimulatory effect on promoter sequence. assumed crucial lily-petal pigmentation. yeast two-hybrid that diverged from typical factor behavior it did not form complex with MYB regulate This discrepancy could be attributed deletion N-terminal conserved sequence LvbHLH13. study provides new candidate bHLH-MYB partner explore regulatory network research opportunities for breeding lilies various contents. These findings lay theoretical foundation subsequent investigations into flower coloring mechanisms.

Language: Английский

Citations

4
Rosa1, a Transposable Element-Like Insertion, Produces Red Petal Coloration in Rose Through Altering RcMYB114 Transcription DOI Creative Commons
Maofu Li, Hui Zhang, Yuan Yang

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: April 29, 2022

Rose (Rosa sp.) flowers have a rich diversity of colors resulting from the differential accumulation anthocyanins, flavonols, and carotenoids. However, genetic molecular determinants red-petal trait in roses remains poorly understood. Here we report that transposable element-like insertion (Rosa1) into RcMYB114, R2R3-MYB transcription factor's promoter region causes its transcription, red petals. In varieties, RcMYB114 is expressed specifically flower organs, but absent non-red varieties. Sequencing, yeast two-hybrid, transient transformation, activity assays independently confirmed role Rosa1 altering RcMYB114's downstream effects on color. Genetic evidence insertion, which previously unknown DNA element, different those other plants reliable marker to screen roses.

Language: Английский

Citations

19
The R2R3-MYB transcription factor GaPC controls petal coloration in cotton DOI Creative Commons

Caiping Cai,

Fan Zhou, Weixi Li

et al.

The Crop Journal, Journal Year: 2023, Volume and Issue: 11(5), P. 1319 - 1330

Published: April 30, 2023

Although a few cases of genetic epistasis in plants have been reported, the combined analysis genetically phenotypic segregation and related molecular mechanism remains rarely studied. Here, we identified gene (named GaPC) controlling petal coloration Gossypium arboreum following heritable recessive epistatic model. Petal is controlled by single dominant gene, GaPC. A loss-of-function mutation GaPC leads to Gapc that masks phenotype other color genes shows interactions. Map-based cloning showed encodes an R2R3-MYB transcription factor. 4814-bp long terminal repeat retrotransposon insertion at second exon led loss function disabled coloration. regulating anthocyanin flavone biosynthesis pathways. Expression core phenylpropanoid pathways was higher colored than white petals. conferred flavonoids anthocyanins, with red yellow petals rich flavonol glycosides, respectively. This study provides new insight on epistasis, also has potential breeding value engineering develop or fibers for multi-functional utilization cotton.

Language: Английский

Citations

10