Transcriptome Comparison between Resistant and Susceptible Soybean Cultivars in Response to Inoculation of Phytophthora sojae DOI Creative Commons
Sangrea Shim, In Jeong Kang,

Hee Jin You

et al.

The Plant Pathology Journal, Journal Year: 2024, Volume and Issue: 40(6), P. 641 - 655

Published: Nov. 29, 2024

Phytophthora root and stem rot, caused by sojae, considerably reduces soybean yield worldwide. Our previous study identified two genomic regions on chromosome 18 (2.1-2.6 53.1-53.3 Mbp) that confer resistance to the P. sojae isolate 2457, through linkage analysis using progenies derived from Daepung × Socheong2 population. These contained 51 19 annotated genes, respectively. However, specific gene responsible for 2457 has yet be identified. In this study, we performed a comparative transcriptomic of Daepung, Korean varieties as resistant susceptible RNA sequencing was conducted tissue samples collected at 0, 6, 12 hours after inoculation (HAI), significant differences in expression defense-related genes were observed across time points between cultivars. Genes associated with jasmonic acid, salicylic ethylene, systemic acquired pathways upregulated both cultivars 6 HAI compared 0 HAI, these biological processes more strongly HAI. A comparison differentially expressed (DEGs) candidate within previously QTL revealed an ortholog HS1 PRO-1 2 Arabidopsis thaliana among DEGs Socheong2, particularly This will aid targeted breeding efforts develop improved sojae.

Language: Английский

Isoflavonoid metabolism in leguminous plants: an update and perspectives DOI Creative Commons
Qilin Yang, Guodong Wang

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 9, 2024

Isoflavonoids constitute a well-investigated category of phenylpropanoid-derived specialized metabolites primarily found in leguminous plants. They play crucial role legume development and interactions with the environment. usually function as phytoalexins, acting against pathogenic microbes nature. Additionally, they serve signaling molecules rhizobial symbiosis. Notably, owing to their molecular structure resembling human estrogen, are recognized phytoestrogens, imparting positive effects on health. This review comprehensively outlines recent advancements research pertaining isoflavonoid biosynthesis, transcriptional regulation, transport, physiological functions, particular emphasis soybean we pose several questions encourage exploration into novel contributors metabolism potential roles plant-microbe interactions.

Language: Английский

Citations

7
GWAS and WGCNA Analysis Uncover Candidate Genes Associated with Oil Content in Soybean DOI Creative Commons

Xunchao Zhao,

Zhang Yan,

Jie Wang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(10), P. 1351 - 1351

Published: May 14, 2024

Soybean vegetable oil is an important source of the human diet. However, analysis genetic mechanism leading to changes in soybean content still incomplete. In this study, a total 227 materials were applied and analyzed by genome-wide association study (GWAS). There are 44 quantitative trait nucleotides (QTNs) that identified as associated with content. A six, four, 34 significant QTN loci Xiangyang, Hulan, Acheng, respectively. Of those, 26 QTNs overlapped or near known locus (QTL), 18 new related identified. 594 genes located peak single nucleotide polymorphism (SNP) from three tested environments. These candidate exhibited enrichment tropane, piperidine, pyridine alkaloid biosynthesiss (ko00960), ABC transporters (ko02010), photosynthesis-antenna proteins (ko00196), betalain biosynthesis (ko00965). Combined GWAS weighted gene co-expression network (WGCNA), four (Glyma.18G300100, Glyma.11G221100, Glyma.13G343300, Glyma.02G166100) may regulate addition, Glyma.18G300100 was divided into two main haplotypes studied accessions. The haplotype 1 significantly lower than 2. Our research findings provide theoretical basis for improving regulatory

Language: Английский

Citations

7
Hub Genes and Pathways Related to Lemon (Citrus limon) Leaf Response to Plenodomus tracheiphilus Infection and Influenced by Pseudomonas mediterranea Biocontrol Activity DOI Open Access
Angelo Sicilia, Riccardo Russo, Vittoria Catara

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2391 - 2391

Published: Feb. 17, 2024

The lemon industry in the Mediterranean basin is strongly threatened by “mal secco” disease (MSD) caused fungus Plenodomus tracheiphlilus. Leaf pretreatments with Pseudomonas mediterranea 3C have been proposed as innovative tools for eco-sustainable interventions aimed at controlling disease. In this study, exploiting results of previously performed RNAseq analysis, WCGNA was conducted among gene expression patterns both inoculated (Pt) and pretreated fungus-inoculated plants (Citrus limon L.) (3CPt), two indicators fungal infection, i.e., amount DNA measured planta index (DI). aims work were (a) to identify modules significantly associated those traits, (b) construct co-expression networks related mal secco disease; (c) define effect action mechanisms P. comparing networks. led identification nine hub genes networks, three them belonging receptor-like kinases (RLK), such HERK1, CLAVATA1 LRR, which play crucial roles plant–pathogen interaction. Moreover, comparison between indicated that receptors not induced presence mediterranea, suggesting how powerful discovering must undergo further investigation be eventually knocked out.

Language: Английский

Citations

5
Identification of Genes Responsible for the Synthesis of Glycitein Isoflavones in Soybean Seeds DOI Creative Commons
Masaki Horitani,

Risa Yamada,

Kanami Taroura

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 156 - 156

Published: Jan. 5, 2024

Soybean (Glycine max (L.) Merrill) isoflavones are among the most important secondary metabolites, with functional benefits for human health. Soybeans accumulate three aglycone forms of isoflavones: genistein, daidzein, and glycitein. landrace Kumachi-1 does not malonylglycitin at all. Gene structure analysis indicated that Glyma.11G108300 (F6H4) has a 3.8-kbp insertion, resulting in truncated flavonoid 6-hydroxylase (F6H) sequence compared to wild-type Fukuyutaka. Mapping experiments using mutant line (MUT1246) phenotype similar Kumachi-1, single-nucleotide polymorphism (SNP) F6H4, revealed co-segregation this mutation absence glycitein isoflavones. We also identified (K01) exhibited change HPLC retention time isoflavones, accumulating glycoside malonylglycoside 6-hydroxydaidzein. K01 contains an SNP produces premature stop codon Glyma.01G004200 (IOMT3), novel soybean isoflavone O-methyltransferase (IOMT) gene. further analyzed transgenic hairy roots soybeans expressing (IOMT3). Those overexpressing F6H4 accumulated 6-hydroxydaidzein (M_6HD), co-expression IOMT3 increased level but M_6HD. These results indicate responsible biosynthesis seed hypocotyl.

Language: Английский

Citations

3
Combined dynamic transcriptome and flavonoid metabolome reveal the role of Mo nanoparticles in the nodulation process in soybean DOI

Yining Liu,

Songwei Wu,

Xiaoming Qin

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 945, P. 173733 - 173733

Published: June 7, 2024

Language: Английский

Citations

3
An R2R3‐type MYB transcription factor, GmMYB77, negatively regulates isoflavone accumulation in soybean [Glycine max (L.) Merr.] DOI
Yitian Liu, Shengrui Zhang, Jing Li

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Summary Soybean [ Glycine max (L.) Merr.] is an exceptionally rich in isoflavones, and these compounds attach to oestrogen receptors the human body, lessening risk of breast cancer effectively alleviating menopausal syndrome symptoms. Uncovering molecular mechanisms that regulate soybean isoflavone accumulation crucial for enhancing production compounds. In this study, we combined bulk segregant analysis sequencing (BSA‐seq) a genome‐wide association study (GWAS) discover novel R2R3‐MYB family gene, GmMYB77 , regulates soybean. Using hairy root transient expression system, verified inhibits accumulation. Furthermore, knocking out significantly increased total (TIF) content, particularly malonylglycitin, while its overexpression resulted notable decrease contents malonylglycitin TIF. We found can directly binds core sequence GGT suppresses key biosynthesis genes Isoflavone synthase 1 ( GmIFS1 ), 2 GmIFS2 Chalcone 7 GmCHS7 ) 8 GmCHS8 by using dual‐luciferase assays, electrophoretic mobility shift assays yeast one‐hybrid experiments. Natural variations promoter region affect expression, thereby regulating TIF contents. Hap‐P2, elite haplotype, plays pivotal role breeding substantially enhanced content. These findings enhance our understanding influencing content provide valuable genetic resource efforts future.

Language: Английский

Citations

3
Genome-Wide Association Study Reveals Influence of Cell-specific Gene Networks on Soybean Root System Architecture DOI Creative Commons
Ying Sun,

Charlotte Miller,

Ashish B. Rajurkar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 29, 2024

Abstract Root system architecture (RSA) describes the shape and arrangement of a plant’s roots in soil including angle, rate growth, type individual roots, which facilitates uptake nutrients water. In crop improvement efforts, RSA has been less well studied due to technical challenges associated with phenotyping as focus on above-ground traits such yield. We developed gel-based root called RADICYL (Root Architecture 3D Cylinder), is non-invasive, high-throughput approach that enabled us measure 15 traits. leveraged perform comprehensive genome-wide association study (GWAS) panel 371 diverse soybean elite lines, cultivars, landraces, closely related species identify gene networks underlying RSA. identified 54 significant single nucleotide polymorphisms (SNPs) our GWAS, some were shared across multiple while others specific given trait. generated cell atlas using nuclei RNA sequencing (snRNAseq) explore genes context tissues. Using co-expression network (GCN) analyses applied RNA-seq tissues, we network-level associations predominantly expressed endodermis width, those metaphloem lateral length. Our results suggest pathways active cell-types influence architecture.

Language: Английский

Citations

2
Joint GWAS and WGCNA Identify Genes Regulating the Isoflavone Content in Soybean Seeds DOI

Zhenhong Yang,

Xu Wu,

Yina Zhu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(33), P. 18573 - 18584

Published: Aug. 6, 2024

Isoflavone is a secondary metabolite of the soybean phenylpropyl biosynthesis pathway with physiological activity and beneficial to human health. In this study, isoflavone content 205 germplasm resources from 3 locations in 2020 showed wide phenotypic variation. A joint genome-wide association study (GWAS) weighted gene coexpression network analysis (WGCNA) identified 33 single-nucleotide polymorphisms 11 key genes associated content. Gene ontology enrichment analysis, coexpression, haplotype revealed natural variations

Language: Английский

Citations

2
Bioinformatics Identification and Expression Analysis of Acetyl-CoA Carboxylase Reveal Its Role in Isoflavone Accumulation during Soybean Seed Development DOI Open Access

Xu Wu,

Zhenhong Yang,

Yina Zhu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10221 - 10221

Published: Sept. 23, 2024

Isoflavones belong to the class of flavonoid compounds, which are important secondary metabolites that play a crucial role in plant development and defense. Acetyl-CoA carboxylase (ACCase) is biotin-dependent enzyme catalyzes conversion into Malonyl-CoA plants. It key fatty acid synthesis also production various metabolites. However, information on ACC gene family soybean (Glycine max L. Merr.) genome specific members involved isoflavone biosynthesis still lacking. In this study, we identified 20 genes (GmACCs) from further characterized their evolutionary relationships expression patterns. Phylogenetic analysis showed GmACCs could be divided five groups, structures within same groups were highly conserved, indicating they had similar functions. The randomly distributed across 12 chromosomes, collinearity suggested many originated tandem segmental duplications, with these being under purifying selection. addition, pattern indicated there was functional divergence among different tissues. reached peak levels during early or middle stages seed development. Based transcriptome content data, weighted co-expression network constructed, three candidate (Glyma.06G105900, Glyma.13G363500, Glyma.13G057400) may positively regulate identified. These results provide valuable for characterization application soybean.

Language: Английский

Citations

2
Cytochrome GmGLY1 is Involved in the Biosynthesis of Glycitein in Soybean DOI
Peipei Zhang,

Changyun Yang,

Jiao Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 10944 - 10957

Published: May 6, 2024

Isoflavones, the major secondary metabolites of interest due to their benefits both human and plant health, are exclusively produced by legumes. In this study, we profiled isoflavone content in dry seeds from 211 soybean [Glycine max (L.) Merr.] accessions grown across five environments. Broad discernible phenotypic variations were observed among accessions, regions, years growth. Twenty-six single-nucleotide polymorphisms (SNPs) associated with sum glycitein (GLE), glycitin (GL), 6″-O-acetylglycitin (AGL), 6″-O-malonylglycitin (MGL) contents detected multiple environments via a genome-wide association study (GWAS). These SNPs located on chromosome 11 (8,148,438 bp 8,296,956 bp, renamed qGly11–01). Glyma.11g108300 (GmGLY1), gene that encodes P450 family protein, was identified sequence variation analysis, functional annotation, weighted coexpression network analysis (WGCNA), expression profile candidate gene, hairy roots transformation soybean. Overexpression GmGLY1 increased (GLC) transgenic seeds, while CRISPR/Cas9-generated mutants exhibited decreased GLC daidzein (DAC). Haplotype revealed allelic significantly affect accumulation. findings enhance our understanding genes influencing may guide breeding for lines high stable GLC.

Language: Английский

Citations

1