ABSTRACT
This
study
investigates
the
phytochemical
composition
and
biopharmacological
potential
of
three
Hypericum
species
(
H.
scabrum
,
lysimachioides
uniglandulosum
)
from
Turkey.
Aqueous
hydroalcoholic
extracts
were
analyzed
for
their
total
phenolic
content
(TPC),
flavonoid
(TFC),
individual
components
(by
UHPLC–HRMS
technique).
Antioxidant
activities
investigated
by
DPPH,
ABTS,
CUPRAC,
FRAP,
phosphomolybdenum,
metal
chelating
assays.
The
inhibition
effects
tested
on
acetylcholinesterase
(AChE),
butyrylcholinesterase
(BChE),
tyrosinase,
amylase,
glucosidase
examined.
One
hundred
compounds
identified
in
chemical
composition,
specific
genus
such
as
hyperoside,
hypericin,
pseudohypericin,
detected.
highest
TPC
was
detected
ethanol/water
extract
with
69.21
mg
GAE/g.
Furthermore,
showed
strongest
free
radical
reducing
effect.
more
active
than
water
extracts.
Neuroprotective
assessments
indicated
downregulation
COX‐2
NOS‐2
genes
LPS‐stimulated
mouse
cortex
models,
alongside
modulation
SERT
NET
expression,
suggesting
reduced
neuroinflammation
enhanced
neurotransmitter
release.
Molecular
docking
dynamics
analyses
highlighted
strong
binding
interactions,
especially
NET_hyperoside
NET_myricitrin
complexes.
results
indicate
significant
therapeutic
these
extracts,
supporting
development
natural
agents
against
oxidative
stress,
neuroinflammation,
related
neurodegenerative
diseases.
Nutrients,
Journal Year:
2024,
Volume and Issue:
16(8), P. 1167 - 1167
Published: April 14, 2024
Polygonati
Rhizoma
(PR)
has
certain
neuroprotective
effects
as
a
homology
of
medicine
and
food.
In
this
study,
systematic
pharmacology,
molecular
docking,
in
vitro
experiments
were
integrated
to
verify
the
antidepressant
active
ingredients
PR
their
mechanisms.
A
total
seven
compounds
found
be
associated
with
45
targets
depression.
Preliminarily,
DFV
docking
cyclooxygenase
2
(COX2)
showed
good
affinity.
vitro,
inhibited
lipopolysaccharide
(LPS)-induced
inflammation
BV-2
cells,
reversed
amoeba-like
morphological
changes,
increased
mitochondrial
membrane
potential.
malondialdehyde
(MDA)
overexpression
superoxide
dismutase
(SOD)
expression
inhibition
LPS-induced
cells
decreased
interleukin-1β
(IL-1β),
tumor
necrosis
factor-α
(TNF-α),
IL-6
mRNA
levels
dose-dependent
manner.
both
protein
COX2
induced
by
LPS,
activation
NACHT,
LRR,
PYD
domains-containing
3
(NLRP3)
caspase1
was
suppressed,
thus
exerting
an
effect.
This
study
proves
that
may
important
component
basis
for
play
role.
Frontiers in Psychiatry,
Journal Year:
2024,
Volume and Issue:
15
Published: Aug. 23, 2024
Background
The
incidence
rate
of
adolescent
depression
and
anxiety
has
been
increasing
since
the
outbreak
COVID-19,
which
there
are
no
effective
therapeutic
drugs
available.
Si-ni
San
is
commonly
used
in
traditional
Chinese
medicine
for
treatment
depression-like
as
well
anxiety-like
behavior,
but
its
mechanism
treating
combined
with
during
adolescence
not
yet
clear.
Methods
Network
pharmacology
was
to
explore
potential
drug
molecules
related
targets,
molecular
docking
dynamics
(MD)
simulation
were
evaluate
interaction
between
a
model
rats
following
behavioral
tests
biology
verify
results
from
network
docking.
Results
As
result,
256
active
ingredients
1128
targets
screened
out.
Among
them,
quercetin,
Luteolin,
kaempferol,
7-Methoxy-2-methyl
isoflavone,
formononetin
showed
be
most
ingredients;
while
STAT3,
IL6,
TNF,
AKT1,
TP53,
IL1B,
MAPK3,
VEGFA,
CASP3,
MMP9
targets.
AGE-RAGE
signaling
pathway
diabetic
complications,
IL-17
pathway,
HIF-1
PI3K-Akt
TNF
involved
anti-inflammation
processes,
probable
pathways
regulated
by
San.
Molecular
MD
compounds
inflammation-associated
revealed
good
binding
abilities
nobiletin
PTGS2
PPARγ.
In
experiment
rats,
markedly
suppressed
early
maternal
separation
(MS)
chronic
unpredictable
mild
stress
(CUMS)-induced
anxiety.
qPCR
further
indicated
that
oxidative
inflammatory
response.
Conclusion
This
study
demonstrates
anxiety-
behavior
induced
MS
CUMS
can
ameliorated
improved
inflammation
hippocampus
via
targeting
Nrf2
helping
reveal
ACS Central Science,
Journal Year:
2024,
Volume and Issue:
10(5), P. 1105 - 1114
Published: April 25, 2024
Cyclooxygenase-2
(COX-2)
is
an
enzyme
that
plays
a
pivotal
role
in
peripheral
inflammation
and
pain
via
the
prostaglandin
pathway.
In
central
nervous
system
(CNS),
COX-2
implicated
neurodegenerative
psychiatric
disorders
as
potential
therapeutic
target
biomarker.
However,
clinical
studies
with
have
yielded
inconsistent
results,
partly
due
to
limited
mechanistic
understanding
of
how
activity
relates
CNS
pathology.
Therefore,
developing
positron
emission
tomography
(PET)
radiotracers
for
human
neuroimaging
interest.
This
study
introduces
[11C]BRD1158,
which
potent
uniquely
fast-binding,
selective
PET
radiotracer.
[11C]BRD1158
was
developed
by
prioritizing
potency
at
COX-2,
isoform
selectivity
over
COX-1,
fast
binding
kinetics,
free
fraction
brain.
Evaluated
through
vivo
rodent
models
overexpression,
demonstrated
high
brain
uptake,
target-engagement,
functional
reversibility,
excellent
specific
binding,
advantageous
imaging
applications.
Lastly,
post-mortem
samples
from
Huntington's
disease
(HD)
patients
preclinical
HD
mouse
showed
levels
were
elevated
specifically
disease-affected
regions,
primarily
increased
expression
microglia.
These
findings
indicate
holds
promise
novel
marker
onset
progression,
one
many
applications
PET.
Journal of Integrative Neuroscience,
Journal Year:
2024,
Volume and Issue:
23(12)
Published: Dec. 24, 2024
The
coexistence
of
anxiety
or
depression
with
coronary
heart
disease
(CHD)
is
a
significant
clinical
challenge
in
cardiovascular
medicine.
Recent
studies
have
indicated
that
hypothalamic-pituitary-adrenal
(HPA)
axis
activity
could
be
promising
focus
understanding
and
addressing
the
development
treatments
for
comorbid
CHD
depression.
HPA
helps
to
regulate
levels
inflammatory
factors,
thereby
reducing
oxidative
stress
damage,
promoting
platelet
activation,
stabilizing
gut
microbiota,
which
enhance
survival
regeneration
neurons,
endothelial
cells,
other
cell
types,
leading
neuroprotective
cardioprotective
benefits.
This
review
addresses
relevance
nervous
systems,
as
well
latest
research
advancements
regarding
its
mechanisms
action.
discussion
includes
detailed
function
regulating
processes
mentioned.
Above
all,
it
summarizes
therapeutic
potential
biomarker
atherosclerotic
combined
