Fine Mapping and Identification of a Fusarium Wilt Resistance Gene FwS1 in Pea DOI Creative Commons

Dong Deng,

Suli Sun, Wenqi Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Abstract Pea Fusarium wilt, incited by Fusarium oxysporum f. sp. pisi (Fop), has always been a devastating disease that causes severe yield losses and economic damage in pea-growing regions worldwide. The utilization of pea cultivars carrying resistance gene is the most efficient approach for managing this disease. In order to finely map gene, F2 population was established through cross between Shijiadacaiwan 1 (resistant) Y4 (susceptible). genetic analysis indicated Fop governed single dominant named as FwS1. Based on bulked segregant sequencing (BSA-seq) analyses, FwS1 initially detected chromosome 6 (i.e., linking group Ⅱ, chr6LG2), subsequent linkage mapping with 589 individuals fine-mapped FwS1 into 91.4 kb region. further functional annotation haplotype confirmed Psat6g003960, characterized NB-ARC (nucleotide-binding adaptor shared APAF-1, R proteins, CED-4) domain, considered promising candidate gene. encoding amino acids were altered “T/C” single-nucleotide polymorphism (SNP) first exon resulting observed differences peas. SNP locus, molecular marker A016180 determined be diagnostic validating its specificity both accessions populations different backgrounds. with KASP could facilitate marker-assisted selection breeding pea. addition, upon comparing gene Psat6g003960 74SN3B SJ1, it noted their sequences are identical, suggesting Fwf may same against wilt.

Language: Английский

Identification of significant marker-trait associations for Fusarium wilt resistance in a genetically diverse core collection of safflower using AFLP and SSR markers DOI
Krishna Nand Singh, Sapna Rawat,

Kuldeep Kumar

et al.

Journal of Applied Genetics, Journal Year: 2022, Volume and Issue: 63(3), P. 447 - 462

Published: May 6, 2022

Language: Английский

Citations

9
Screening for Pea Germplasms Resistant to Fusarium Wilt Race 5 DOI Creative Commons

Dong Deng,

Suli Sun, Wenqi Wu

et al.

Agronomy, Journal Year: 2022, Volume and Issue: 12(6), P. 1354 - 1354

Published: June 1, 2022

Fusarium wilt (FW), caused by oxysporum f. sp. pisi (Fop), has always been an important disease affecting pea production and causing severe yield losses in most pea-growing areas worldwide. Growing resistant cultivars is the economical effective method for controlling disease. In this study, firstly, 21 isolates were identified as races 1 5 of Fop based on morphological molecular characteristics, reactions seven differential cultivars. Then, a detailed resistance evaluation strategy was established validated death rate score, index, percentage leaves showing symptoms each individual plant. Finally, 1311 germplasm collection including 740 accessions from China 571 aboard or unknown sources evaluated to representative isolate PF22b race 5, results showed that 28 164 highly (HR) (R), respectively. Among these accessions, 13 HR 44 R collected 19 provinces China, which came Sichuan, Tibet, Gansu Provinces. The 15 120 10 countries outside sources, majority United States, Australia, Russia. findings would provide information using control wilt. Incorporating into breeding programs will contribute improving

Language: Английский

Citations

8
Size-Dependent Disease Resistance Enhancement of Hollow Mesoporous Silica Nanoparticles in Cowpea Plant Involved in Salicylic Acid Mediated Systemic Acquired Resistance for Fusarium Wilt Control DOI Open Access

Chaopu Ding,

Yunfei Zhang,

Chongbin Chen

et al.

Published: Feb. 27, 2024

In agriculture, soil-borne fungal pathogens, especially Fusarium oxysporum strains, are posing a serious threat to efforts achieve global food security. the search for safer agrochemicals, silica nanoparticles (SiO2NPs) have recently been proposed as new tool alleviate pathogen damage including wilt. Hollow mesoporous (HMSNs), unique class of SiO2NPs, widely accepted desired pesticide carrier. However, their role in enhancing disease resistance and specific mechanism remain unknown. this study, three sizes HMSNs (19, 96 406 nm HMSNs–19, HMSNs–96 HMSNs–406, respectively) were synthesized characterized determine effects on seed germination, seedling growth, f. sp. phaseolus (FOP) suppression cowpea roots by foliar spray using phenotypes, fresh biomass progression indicators. The results revealed that exhibited no adverse impacts germination tended improve plant growth. Also, they exert FOP with size- concentration-dependent manner. HMSNs–406 possessed best control effect at concentration 1000 mg/L showing an upto 40.00% decline severity. Si(OH)4 was also effective lower 100 mg/L, whereas its higher concentrations obviously control, Moreover, we conformed posed wilt activating SA (salicylic acid)-dependent SAR (systemic acquired resistance) responses rather than directly suppressing FOP. A level content elevated expression maker genes PR-1 PR-5 treated provided substantial evidences mode action. Other resistance-related genes, well defense-responsive enzymes, involved HMSNs-activated pathway. Overall, first time, our extended potent elicitor serve versatile alternative protection low cost, highly efficiency sustainability.

Language: Английский

Citations

1
Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum DOI Creative Commons
Abhaya Kumar Sahu, Punam Kumari,

Bhabatosh Mittra

et al.

Stress Biology, Journal Year: 2024, Volume and Issue: 4(1)

Published: April 9, 2024

Susceptibility is defined as the disruption of host defence systems that promotes infection or limits pathogenicity. Glutathione (GSH) a major component signalling pathways maintain redox status and synthesised by γ-glutamyl cysteine synthetase (γ-ECS). On other hand, lignin acts barrier in primary cell wall vascular bundles (VBs) phenylalanine ammonia-lyase (PAL) intracellular system plants. In this study, we used two inhibitors, such L-Buthionine-sulfoximine (BSO), which irreversibly inhibits γ-ECS, 2,4-dichlorophenoxyacetic acid (DPA), reduces PAL activity leads to induction oxidative stress wheat (Triticum aestivum) seedlings after exposure Fusarium oxysporum. Seedlings treated with 1 mM L-BSO 2,4-DPA showed high levels hydrogen peroxide (H

Language: Английский

Citations

1
Fine Mapping and Identification of a Fusarium Wilt Resistance Gene FwS1 in Pea DOI Creative Commons

Dong Deng,

Suli Sun, Wenqi Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Abstract Pea Fusarium wilt, incited by Fusarium oxysporum f. sp. pisi (Fop), has always been a devastating disease that causes severe yield losses and economic damage in pea-growing regions worldwide. The utilization of pea cultivars carrying resistance gene is the most efficient approach for managing this disease. In order to finely map gene, F2 population was established through cross between Shijiadacaiwan 1 (resistant) Y4 (susceptible). genetic analysis indicated Fop governed single dominant named as FwS1. Based on bulked segregant sequencing (BSA-seq) analyses, FwS1 initially detected chromosome 6 (i.e., linking group Ⅱ, chr6LG2), subsequent linkage mapping with 589 individuals fine-mapped FwS1 into 91.4 kb region. further functional annotation haplotype confirmed Psat6g003960, characterized NB-ARC (nucleotide-binding adaptor shared APAF-1, R proteins, CED-4) domain, considered promising candidate gene. encoding amino acids were altered “T/C” single-nucleotide polymorphism (SNP) first exon resulting observed differences peas. SNP locus, molecular marker A016180 determined be diagnostic validating its specificity both accessions populations different backgrounds. with KASP could facilitate marker-assisted selection breeding pea. addition, upon comparing gene Psat6g003960 74SN3B SJ1, it noted their sequences are identical, suggesting Fwf may same against wilt.

Language: Английский

Citations

1