The Imperative Use of Bacillus Consortium and Quercetin Contributes to Suppress Fusarium Wilt Disease by Direct Antagonism and Induced Resistance DOI Creative Commons
Ali Hassan, Waheed Akram, Humaira Rizwana

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(10), P. 2603 - 2603

Published: Oct. 21, 2023

Fusarium wilt diseases severely influence the growth and productivity of numerous crop plants. The consortium antagonistic rhizospheric Bacillus strains quercetin were evaluated imperatively as a possible remedy to effectively manage disease tomato selection was made based on in-vitro bioassays against oxysporum f.sp. lycoprsici (FOL). Quercetin selected after screening library phytochemicals during in-silico molecular docking analysis using LysM receptor kinases "SILKY12" its dual role in symbiosis plant defense responses. After test materials, pot trials conducted where plants provided soil drenching foliar spray different concentrations. combined application (Bacillus velezensis strain BS6, thuringiensis BS7, fortis BS9) (1.0 mM) reduced index up 69%, also resulting increased attributes. Likewise, imperative significantly total phenolic contents activities enzymes phenylpropanoid pathway. Non-targeted metabolomics performed investigate perturbation metabolites. FOL pathogen negatively affected range metabolites including carbohydrates, amino acids, phenylpropanoids, organic acids. Thereinto, treatment ameliorated production These findings prove use an effective sustainable promote under stress conditions.

Language: Английский

Uniting the Role of Endophytic Fungi against Plant Pathogens and Their Interaction DOI Creative Commons

Shazia Akram,

Ayesha Ahmed,

Pengfei He

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(1), P. 72 - 72

Published: Jan. 3, 2023

Endophytic fungi are used as the most common microbial biological control agents (MBCAs) against phytopathogens and ubiquitous in all plant parts. Most of fungal species have roles a variety pathogens. Fungal endophytes provide different services to be pathogen agents, using an important aspect form enhanced growth induced systemic resistance, produce antifungal secondary metabolites (lipopeptides, antibiotics enzymes) through colonization, compete with other pathogenic microorganisms for factors (space nutrients). The purpose this review is highlight potential properties We focused on introduction, biology, isolation, identification endophytic fungi, their activity endosymbionts developed specific genes that exhibited behavior demonstrated defensive responses pathogens such antibiosis, parasitism, lytic enzyme competition, siderophore production, indirect by resistance (ISR) host plant. Finally, microscopic detection techniques study interactions (endophytic interactions) plants briefly discussed.

Language: Английский

Citations

102
Ralstonia solanacearum, a deadly pathogen: Revisiting the bacterial wilt biocontrol practices in tobacco and other Solanaceae DOI
Waqar Ahmed, Jun Yang,

Yujiao Tan

et al.

Rhizosphere, Journal Year: 2022, Volume and Issue: 21, P. 100479 - 100479

Published: Jan. 26, 2022

Language: Английский

Citations

78
Antimicrobial Metabolites of Bacillus velezensis FZB42 Reshape Rice Rhizosphere Microbial Community Composition and Induce Host Resistance Against Rhizoctonia solani DOI Creative Commons
Qurban Ali,

Abdur Rashid Khan,

Yujie Wang

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: 41, P. 100440 - 100440

Published: Jan. 15, 2025

Language: Английский

Citations

2
Plant-Microbe Interaction: Mining the Impact of Native Bacillus amyloliquefaciens WS-10 on Tobacco Bacterial Wilt Disease and Rhizosphere Microbial Communities DOI Creative Commons
Waqar Ahmed,

Zhenlin Dai,

Jinhao Zhang

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(4)

Published: Aug. 1, 2022

The plant rhizosphere acts as the first line of defense against invasion pathogens. perturbation in microbiome is directly related to health and disease development.

Language: Английский

Citations

68
Bacillus amyloliquefaciens WS-10 as a potential plant growth-promoter and biocontrol agent for bacterial wilt disease of flue-cured tobacco DOI Creative Commons
Waqar Ahmed,

Guisu Zhou,

Jun Yang

et al.

Egyptian Journal of Biological Pest Control, Journal Year: 2022, Volume and Issue: 32(1)

Published: March 19, 2022

Abstract Background Bacterial wilt disease caused by the soilborne bacterium Ralstonia solanacearum is a serious threat to flue-cured tobacco production. In this study, an indigenous suppressive Bacillus strain was isolated from rhizosphere soil of healthy plants, and its biocontrol plant growth promoting (PGP) potential were evaluated in in-vivo in-vitro assays. Results Through isolation screening 250 isolates, WS-10 found be best candidate antagonistic against R. (WS-001). In-vitro assays revealed that ( amyloliquefaciens ) showed effective activity WS-001 several plant-pathogenic fungi. As promising PGP rhizobacteria, had ability nitrogen fixation, solubilization inorganic potassium phosphate, biosynthesis indole-3-acetic. co-culture assay, it significantly inhibits WS-001. Our greenhouse experiments physicochemical properties accumulation dry matter contents different parts (roots, stems, leaves) increased presence B. WS-10. The treated with displayed higher values average well color development index, utilization 6 types carbon sources microorganisms, diversity indices microbial communities. planta reduced bacterial incidence up 73.36, 43.82, 86.82% under three treatments improving functional biological community. Conclusions Obtained findings suggested excellent as growth-promoting agent due multiple beneficial traits nutrient suppression. Thus, we conclude high for production crop.

Language: Английский

Citations

42
Seed coat treatment by plant-growth-promoting rhizobacteria Lysobacter antibioticus 13–6 enhances maize yield and changes rhizosphere bacterial communities DOI

Zhenlin Dai,

Waqar Ahmed, Jun Yang

et al.

Biology and Fertility of Soils, Journal Year: 2023, Volume and Issue: 59(3), P. 317 - 331

Published: Feb. 11, 2023

Language: Английский

Citations

33
Pre-soil fumigation with ammonium bicarbonate and lime modulates the rhizosphere microbiome to mitigate clubroot disease in Chinese cabbage DOI Creative Commons
Jinhao Zhang,

Xinghai Zhou,

Yü Zhang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 15, 2024

Plasmodiophora brassicae is an ever-increasing threat to cruciferous crop production worldwide. This study investigated the impact of pre-soil fumigation with ammonium bicarbonate (N) and lime (NB) manage clubroot disease in Chinese cabbage through 16S rRNA gene amplification sequencing. We found that soil N NB suppressed incidence by reducing acidity population P. rhizosphere. Minimum maximum relative control effect about 74.68 66.28% were achieved greenhouse field experiments, respectively, under combined application (LNB) as compared N, NB, (GZ). Microbial diversity analysis Miseq sequencing proved LNB clearly manipulated rhizosphere microbial community composition changed structure microbes GZ. Bacterial phyla such Proteobacteria, Bacteriodetes, Acidobacteria fungal including Olpidiomycota Ascomycota most dominant plants. Soil significantly reduced abundance pathogen at genus (Plasmodiophora) level GZ, while decreased further LNB. co-occurrence network showed a highly connected complex less competition for resources among conclude environmentally friendly sustainable agriculture, plays crucial role mitigating alleviating pH, population, manipulating microbiome.

Language: Английский

Citations

14
Comparative transcriptome analysis reveals molecular mechanisms of resistance in Chinese cabbage to Plasmodiophora brassicae pathotype 11 DOI Creative Commons
Yue Qiu, Jinhao Zhang,

C. Y. Deng

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Background and aims Clubroot caused by the soilborne obligate parasite Plasmodiophora brassicae , is a devastating disease of Chinese cabbage other crucifers. The innate diversity adaptability this pathogen pose significant challenges to effective control measures. However, varied response mechanisms exhibited hosts pathotype 11 at molecular level are still unclear. Methods results This study investigated resistance underlying mechanism two ( Brassica rapa ) varieties (JP 83-1) P. through comparative transcriptome analysis microscopic study. Results demonstrated that 14 days after inoculation (dai) critical time point infection process for resistant variety inhibit proliferation brassica . Although highly JP did not exhibit complete immune 11, it against restricting its in xylem vessels. Microscopic 21 dai revealed cultivar (JP) root structure remained largely unaffected, while roots susceptible (83-1) tissue distortion gall formation, underscoring effectiveness mechanisms. Comparative substantial differences number types differentially expressed genes (DEGs) between cultivars, highlighting key pathways involved response. In (JP), total 9,433 DEGs were identified, with 4,211 up-regulated 5,222 down-regulated. contrast, 6,456 DEGs, 2,781 3,675 showed pronounced activation hormone signaling, cell wall, secondary metabolism, redox state, signaling process. Therefore, our speculation revolves around potential variety, which inhibits via metabolites, ROS also regulates physiological mediated plant hormones such as ABA adapt adverse environmental conditions water scarcity induced pathogen. Conclusion unveils intricate defense potentially activated within when confronted offering valuable insights breeding programs development novel strategies managing clubroot crops. Furthermore, highlights pivotal role host-specific underlie 11.

Language: Английский

Citations

1
Control Strategies of Clubroot Disease Caused by Plasmodiophora brassicae DOI Creative Commons

Christine Struck,

Stefanie Rüsch,

Becke Strehlow

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(3), P. 620 - 620

Published: March 14, 2022

The clubroot disease caused by the soil-borne pathogen Plasmodiophora brassicae is one of most important diseases cruciferous crops worldwide. As with many plant pathogens, spread closely related to cultivation suitable host plants. In addition, temperature and water availability are crucial determinants for occurrence reproduction disease. Current global changes contributing widespread incidence On hand, trade high prices leading an increase in rapeseed other climate change improving living conditions P. temperate climates its increased occurrence. Well-known ways control efficiently this include arable farming strategies: growing plants wide crop rotations, liming contaminated soils, using resistant Since chemical not possible or ecologically compatible, more alternative options being investigated. review, we address challenges control, a focus on biological options.

Language: Английский

Citations

35
Microbial Cross-Talk: Dissecting the Core Microbiota Associated With Flue-Cured Tobacco (Nicotiana tabacum) Plants Under Healthy and Diseased State DOI Creative Commons
Waqar Ahmed,

Zhenlin Dai,

Qi Liu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: April 14, 2022

Bacterial wilt caused by Ralstonia solanacearum is a devastating disease of flue-cured tobacco production which poses significant yield losses all around the world. In this study, we evaluated rhizosphere microbiome healthy and bacterial wilt-infected (diseased) plants through amplification V3-V4 ITS1-5f variable regions 16S internal transcribed spacer (ITS) rRNA. The study was based on location (Qujing, Shilin, Wenshan), plant components (rhizosphere soil roots), sample types (healthy diseased) to assess diversity fungal communities. communities present in roots primarily emanated from soil. Healthy exhibit high microbial compared diseased plants. Among three variables, significantly influence communities, whereas harbors higher than roots. phyla Cyanobacteria Proteobacteria were found relative abundance samples, respectively. As far as fungi concerned, Ascomycota Basidiomycota both root. genera such Bacillus, Bradyrhizobium, Ensifer, Neorhizobium, Lysobacter related growth promotion suppressing abilities dominant genera. Analysis at specie-level revealed that most species are pathogenic could provide conducive environment for infection. conclusion, R. influences negatively affects community composition. Altogether, our demonstrates complexity possibly interact with each other (microbe-microbe) host (host-microbe). This cross-talk be helpful induce resistance against disease.

Language: Английский

Citations

29