Deciphering core microbiota in rhizosphere soil and roots of healthy and Rhizoctonia solani-infected potato plants from various locations

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 22, 2024

Black scurf caused by Rhizoctonia solani severely affects potato production. Through amplification of V3-V4 and ITS1-5f variable regions 16S internal transcribed spacer (ITS) rRNA, the study was based on location (Kunming, Qujing, Zhaotong), plant components (rhizosphere soil roots), sample types (healthy diseased) to assess diversity bacterial fungal communities. We found significantly influence microbial diversity, with rhizosphere being more diverse than roots, community in root is mainly derived from soil. Moreover, roots healthy plants exhibit greater compared those infected . Bacterial phyla Actinobacteriota Acidobacteriota were enriched that whereas Proteobacteria Cyanobacteria showed opposite trend. Fungal phylum Ascomycota low relative abundance Basidiomycota genera including Streptomyces , Lysobacter Bacillus Pseudomonas Ensifer Enterobacter Rhizobium group ( Allorhizobium Neorhizobium Pararhizobium ), along such as Aspergillus Penicillium Purpureocillium Gibberella moniliformis have potential ability growth promotion disease resistance. However, most species some are pathogenic could provide a conducive environment for black infection. Interaction within network increased plants, contrasting trend network. Our findings indicate R. alters underscoring complexity interactions between communities promoting health resistance against scurf.

Language: Английский

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Characterization of a broad-spectrum antifungal strain, Streptomyces graminearus STR-1, against Magnaporthe oryzae

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 8, 2024

Fungal diseases such as the devastating rice blast pose severe threats to crop production worldwide. Biological control of caused by fungal pathogens is an environment-friendly approach for safeguarding production. But insufficient availability microbial agents effective against various has hampered development green in crops. In this study, we identified a broad-spectrum antifungal bacterium, Streptomyces graminearus STR-1, showing antagonistic activity diverse including Magnaporthe oryzae , Rhizoctonia solani Fusarium graminearum Ustilaginoidea virens and Bipolaris maydis . Its was relatively stable less affected temperature pH. Evaluation biocontrol STR-1 revealed that prevented controlled disease via eliciting plant immunity suppressing infection-structure development. broth extract inhibited spore germination, likely through inhibiting protein synthesis. Combining LC–MS chromatography analysis antimicrobial compounds purified from extract, together with decoding genomic sequence, 4-oxo-4-[(1-phenylethyl)amino]but-2-enoic acid, 1,3,5-Trimethylpyrazole SMA-1 potential main secondary metabolites associated its effects. This study suggests bacterial strain could be used identifying highly containing other diseases. The application active offers promising measure tackle disease.

Language: Английский

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Screening, identification and evaluation of an acidophilic strain of Bacillus velezensis B4-7 for the biocontrol of tobacco bacterial wilt

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 1, 2024

Tobacco ( Nicotiana tabacum L.) bacterial wilt, caused by Ralstonia solanacearum , is indeed a highly destructive plant disease, leading to substantial damage in tobacco production. While biological control considered an effective measure for managing related research this area has been relatively limited compared other methods. In order discover new potential antagonistic bacteria with high biocontrol efficacy against we conducted analysis of the microbial composition differences between disease-suppressive and disease-conducive soils using Illumina sequencing. As result, successfully isolated six strains from soil that exhibited antibacterial activity . Among these strains, B4-7 showed strongest activity, even at acidic conditions pH 4.0. Based on genome Average Nucleotide Identity (ANI), was identified as Bacillus velezensis greenhouse field trials, strain significantly reduced disease index efficiencies reaching 74.03% 46.88% respectively. Additionally, plant-promoting abilities led 35.27% increase production conditions. Quantitative real-time (qPCR) demonstrated effectively abundance R. rhizosphere. Genome sequencing Liquid Chromatography-Mass Spectrometry (LC-MS) revealed potentially produces various lipopeptide metabolites, such microlactin, bacillaene, difficidin, bacilysin, surfactin. Furthermore, influenced structure rhizosphere community, increasing fungal diversity, while also promoting growth different beneficial microorganisms. addition, enhanced tobacco’s resistance activities defense enzymes, including superoxide dismutase (SOD), phenylalanine ammonia-lyase (PAL), peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO). Collectively, findings suggest B. holds significant can be promising candidate eco-friendly management wilt.

Language: Английский

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Microbial Consortia: An Engineering Tool to Suppress Clubroot of Chinese Cabbage by Changing the Rhizosphere Bacterial Community Composition

Biology, Journal Year: 2022, Volume and Issue: 11(6), P. 918 - 918

Published: June 15, 2022

Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Chinese cabbage (Brassica rapa subsp. pekinensis) production, which results in extensive yield losses. At present, clubroot control mainly depends upon pesticides, provoke food-safety concerns, and the application of sole biocontrol agents cannot successfully disease. In this study, we investigated effect Bacillus cereus BT-23, Lysobacter antibioticus 13-6, capsici ZST1-2 as strains, intra-/inter-genus co-culture, microbial consortia on plant growth, rhizosphere bacterial diversity field experiment. The efficiently controlled incidence with about 65.78%, decreasing soil acidity enhancing (17,662.49 kg/acre). high-throughput sequencing demonstrated that phyla Proteobacteria Bacteroidetes were present high relative abundance cabbage. Furthermore, Firmicutes was found unique phylum CK-H T1-T7, except for CK-D. recovers imbalance indigenous communities. Therefore, conclude can reduce altering structure This study highlights potential an engineering tool devastating soilborne diseases commercial crops.

Language: Английский

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Evaluation of the novel endophytic fungus Chaetomium ascotrichoides 1‐24‐2 from Pinus massoniana as a biocontrol agent against pine wilt disease caused by Bursaphelenchus xylophilus

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(10), P. 4924 - 4940

Published: June 11, 2024

Abstract BACKGROUND Bursaphelenchus xylophilus , the causative agent of pine wilt disease (PWD), is an ever‐increasing threat to Pinus forests worldwide. This study aimed develop biological control PWD by application endophytic fungi isolated from healthy trees. RESULTS We successfully a novel fungal strain 1‐24‐2 branches massoniana . The culture filtrates (CFs) exhibited strong nematicidal activity against xylophilus, with corrected mortality rate 99.00%. Based on morphological and molecular characteristics, was identified as Chaetomium ascotrichoides In in‐planta assay, seedlings (2‐years‐old) treated CFs + wood nematode (T2) showed significant effect 80%. A total 24 toxic compounds were first through gas chromatography–mass spectrometry (GC–MS) analysis, which O ‐methylisourea, 2‐chlorobenzothiazole, 4,5,6‐trihydroxy‐7‐methylphthalide robust binding sites at Tyr119 phosphoethanolamine methyltransferase (PMT) protein docking approach could be used potential for developing effective nematicides. Interestingly, produces volatile organic (VOCs), disturb natural development process B. whose number decreased up 83.32% in treatment group compared also reduced Botrytis cinerea growth 71.01%. CONCLUSION Our results highlight C. promising biocontrol solid report P. exhibiting world. © 2024 Society Chemical Industry.

Language: Английский

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Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola

The Journal of Microbiology, Journal Year: 2022, Volume and Issue: 60(5), P. 496 - 510

Published: March 31, 2022

Language: Английский

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Co-inoculation of antagonistic Bacillus velezensis FH-1 and Brevundimonas diminuta NYM3 promotes rice growth by regulating the structure and nitrification function of rhizosphere microbiome

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 9, 2023

Microbial inoculation with plant growth-promoting microorganisms (PGPMs) is one of the most promising technologies to solve current global challenges. Co-inoculants more efficient and stable than mono-inoculants. However, growth promoting mechanism co-inoculants in complex soil system still poorly understood. In this study, effects on rice, microbiome mono-inoculant Bacillus velezensis FH-1 (F) Brevundimonas diminuta NYM3 (N) co-inoculant FN obtained previous works were compared. Correlation analysis PLS-PM used explore primary different inoculants rice growth. We hypothesized that promoted (i) by themselves, (ii) improving nutrient availability or (iii) regulating rhizosphere system. also assumed had ways The results showed significantly nitrogen absorption slightly increased total microbial network complexity compared F, N control (CK). B. interfered each other’s colonization FN. F N. bacterial community was quite from CK N, while fungal not other treatments. species functions enriched inhibited are part F. correlation (F/N/FN) mainly rather themselves availability. Co-inoculant promotes specifically enhancing nitrification function through enriching related This may provide theoretical guidance for construction application future.

Language: Английский

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Crop Rotation with Marigold Promotes Soil Bacterial Structure to Assist in Mitigating Clubroot Incidence in Chinese Cabbage

Plants, Journal Year: 2022, Volume and Issue: 11(17), P. 2295 - 2295

Published: Sept. 2, 2022

Clubroot caused by Plasmodiophora brassicae is an economically important soilborne disease of Chinese cabbage worldwide. Integrated biological control through crop rotation considered a good management approach to suppress the incidence diseases. In this study, we evaluated effect marigold plant (root exudates, crude extract, and powder) on germination death resting spores P. in vitro assays. Additionally, also performed 16S high throughput sequencing, investigate impact marigold-Chinese soil bacterial community composition, manage devastating pathogen. This study revealed that root powder significantly promoted spores. Under field conditions, with empty period at least 15 days enhanced spores, shifted rhizosphere suppressed clubroot up 63.35%. Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Verrucomicrobia were most dominant phyla present relative levels cabbage. We concluded can reduce next crop. To our knowledge, first comprehensive prevention marigold.

Language: Английский

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Metagenomic Analysis of Garden Soil-Derived Microbial Consortia and Unveiling Their Metabolic Potential in Mitigating Toxic Hexavalent Chromium

Life, Journal Year: 2022, Volume and Issue: 12(12), P. 2094 - 2094

Published: Dec. 13, 2022

Soil microbial communities connect to the functional environment and play an important role in biogeochemical cycle waste degradation. The current study evaluated distribution of core population garden soil Varanasi region Uttar Pradesh, India their metabolic potential for mitigating toxic hexavalent chromium from wastewater. Metagenomes contain 0.2 million reads 56.5% GC content. metagenomic analysis provided insight into relative abundance revealed domination around 200 bacterial species belonging different phyla four archaeal phyla. top 10 abundant genera were Gemmata, Planctomyces, Steroidobacter, Pirellula, Pedomicrobium, Rhodoplanes, Nitrospira Mycobacterium, Pseudonocardia, Acinetobacter. In this study, Gemmata was dominating genera. Euryarchaeota, Parvarchaeota, Crenarchaeota present with low samples. X-ray photoelectric spectroscopy (XPS) indicates presence carbon, nitrogen–oxygen, calcium, phosphorous, silica soil. Soil-derived consortia showed high [Cr (VI)] removal efficiency (99.37%). isolated had bioremediation, thus, could be beneficial design a heavy-metal treatment system.

Language: Английский

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Recent advances in experimental design of synthetic microbial communities for biocontrol application

BioControl, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Language: Английский

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