Use of cryoprotectors and antioxidants in sturgeon semen cryopreservation

Cryobiology, Journal Year: 2023, Volume and Issue: 111, P. 30 - 39

Published: Feb. 14, 2023

Language: Английский

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Phytogenic feed additives as natural antibiotic alternatives in animal health and production: A review of the literature of the last decade

Animal nutrition, Journal Year: 2024, Volume and Issue: 17, P. 244 - 264

Published: March 22, 2024

The use of antibiotics in animal production raises great public safety concerns; therefore, there is an urgent need for the development substitutes antibiotics. In recent decades, plant-derived feed additives have been widely investigated as antibiotic alternatives health and because they exert multiple biological functions are less likely to induce resistance development. This review summarizes research history classification phytogenic their main functions, potential modes action, influencing factors, negative effects. Further, we highlight challenges developing sustainable, safe, affordable livestock production.

Language: Английский

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Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3379 - 3379

Published: Feb. 8, 2023

For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well humans. Nevertheless, the success varies across species, seasons, and latitudes even within same individual. With dawn progressive analytical techniques field genomics, proteomics, metabolomics, new options for more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics spermatozoa that could predict their cryotolerance before freezing process. Understanding changes biology result exposure to low temperatures may contribute development implementation appropriate measures assure high post-thaw quality. Furthermore, an early prediction or cryosensitivity lead establishment customized protocols interconnecting adequate processing procedures, techniques, cryosupplements are most feasible individual needs ejaculate.

Language: Английский

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Advancements in Understanding and Enhancing Antioxidant-Mediated Sperm Cryopreservation in Small Ruminants: Challenges and Perspectives

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 624 - 624

Published: May 21, 2024

Cryopreservation poses significant challenges to the preservation of sperm integrity and function, particularly in small ruminants where cryodamage is pronounced. This review explores molecular mechanisms underlying strategies for improving cryopreservation outcomes, with a focus on role antioxidants. Cryopreservation-induced alterations proteins RNA transcripts critical including motility, capacitation, fertilization, embryo development, are discussed. Proteomic, transcriptomic, epigenomic advancements have provided valuable insights into these mechanisms, offering potential biomarkers predicting freezability enhancing strategies. Combining technologies such as mass spectrometry flow cytometry allows comprehensive understanding cellular changes induced by freezing–thawing process. However, remain optimizing cryoprotectant formulations antioxidant supplementation improve post-thaw fertility. Further research needed explore wider range novel cryoprotectants, antioxidants, media, well validate their efficacy viability function. Additionally, investigations effects epigenetic factors ruminant species warranted advance our preservation. Overall, this highlights importance antioxidants mitigating underscores need continued refine protocols reproductive outcomes ruminants.

Language: Английский

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Antibiotics in semen extenders – a multiplicity of paradoxes

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(5)

Published: March 6, 2024

Addition of antibiotics to semen extenders was taken for granted many years, from the time that commercial artificial insemination in livestock first began decades ago. However, there is now a growing realisation this non-therapeutic utilisation antibacterial agents contrary current recommendations prudent use medical and veterinary professionals are advised follow. Furthermore, not benign, having negative effects on sperm samples, inseminated female, personnel potentially environment. The purpose review three-fold: highlight fact used extenders, with result considerable amounts globally animal breeding, recent studies aspects using purpose, look at possible alternatives. Recent changes legislation regarding occurred some, but all, countries, leaving question marks producers as whether should be added or not.

Language: Английский

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Effect of Some Plant-Based Substances on Microbial Content and Sperm Quality Parameters of Bull Semen

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3435 - 3435

Published: Feb. 8, 2023

The rapid emergence of antibacterial resistance requires alternatives to antibiotics be found, including for semen preservation. One the possible would use plant-based substances with known antimicrobial effects. objective this study was test effect pomegranate powder, ginger, and curcumin extract in two concentrations on bull microbiota after exposure <2 h 24 h. An additional aim evaluate these sperm quality parameters. bacterial count low from beginning; however, a reduction present all tested compared control. A control samples also observed time. Curcumin at concentration 5%, reduced by 32% only substance that had slight positive kinematics. other were associated decline kinematics viability. Neither deleterious viability parameters measured flow cytometry. results indicate 5% can reduce does not have negative influence quality.

Language: Английский

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Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant

Frontiers in Animal Science, Journal Year: 2025, Volume and Issue: 5

Published: Jan. 3, 2025

The objective of the present study was to evaluate protective effects nanoemulsion thyme ( Thymus vulgaris ) essential oil (EO) during liquid state preservation ram semen. Storage media were prepared: one containing (Th), another with its form (NTh), and a control sample without (Dovx). quality semen diluted in prepared after at 4°C 15°C examined by measuring two parameters indicative quality: motility progressivity refer ability sperm cells move actively efficiently, which is crucial for successful fertilization. results showed decrease mobility all mediums, but EO slight decreases compared medium (p&lt;0.05). On other hand, identified negative impact nanoemulsions on catalase concentrations, potentially leading inhibition In addition, significantly (p&lt;0,05) decreased malondialdehyde concentration, increased total protein glucose contents, lactate dehydrogenase activity. A significant calcium content observed (p &lt; 0.05). , combined nanotechnology encapsulation as delivery method (nanoemulsion), demonstrated notable effective role enhancing ovine

Language: Английский

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Enhancement of Semen Cryopreservation from Native Thai Bulls Through Moringa oleifera Leaf Extract Supplementation

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 439 - 439

Published: Feb. 5, 2025

Cryopreservation of native Thai bull semen often results in significant post-thaw quality reduction, underscoring the need for effective cryoprotective strategies. This study investigated effect Moringa oleifera leaf extract (MOLE) as an antioxidant supplementation by incorporating four MOLE concentrations (0–1.5% [w/v]) into a standard extender, followed cryopreservation using liquid nitrogen vapor freezing. Data were analyzed randomized complete block design with Tukey’s post hoc test (p < 0.05). Post-thaw analysis revealed that 1 mg/mL significantly enhanced total sperm motility, progressive viability, and plasma membrane integrity compared to control other concentration also improved amplitude lateral head displacement curvilinear velocity reduced malondialdehyde levels samples 0.05), indicating lipid peroxidation. Higher negatively impacted quality. In conclusion, markedly peroxidation, suggesting its potential enhancing reproductive outcomes bulls.

Language: Английский

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Influence of tannin-rich extract from commercial Acacia mearnsii and gallocatechin on ovine cryopreserved semen viability

Systems Biology in Reproductive Medicine, Journal Year: 2025, Volume and Issue: 71(1), P. 90 - 101

Published: March 14, 2025

The objective of this study was to evaluate the effect a tannin-rich extract from commercial Acacia mearnsii (MTE_0), and gallocatechin, flavonoid compound derived mearnsii, on long-term viability motility cryopreserved ovine semen. Six fresh ejaculates obtained six adult merino rams twice per week for three weeks were allocated five aliquots (0, 12.5, 25, 50, 100 µM gallocatechin added into Optidyl™ extender) before cooling cryopreservation. Effects MTE_0 post-thawed characteristics analyzed using computer-assisted semen analysis (CASA), (LIVE/DEAD® kit, Molecular Invitrogen, Waltham, MA), oxidative stress (2,7-dichlorodihydrofluorescein diacetate (H2DCFDA, Thermo Fisher®, MA)) reactive oxygen species (ROS), mitochondrial membrane potential (JC-1 MitoTracker, acrosomal integrity (lectin PNA), capacitation merocyanine 540 (M540) YO-PRO-1 dyes in flow cytometry. Data one-way ANOVA (IBM SPSS 21.0 Windows, Armonk, NY). Gallocatechin at 25 positively affected (p ≤ .001) kinematic parameters including average path velocity (VAP), progressive (VSL), beat cross frequency (BCF) Similarly, µM- improved sperm (live 21.99 ± 2.06%), reduced ROS levels (26.45 1.10%), mitigated premature (viable stable 20.08 1.48%) compared other treatments. addition resulted significant positive with extract. It is concluded that inclusion significantly reduces deterioration following This first introduce as an efficient antioxidant additive improve its quality during storage. Our findings will help post-thaw longevity. Future studies elucidate mechanism anti-oxidative action derivatives longevity are recommended.

Language: Английский

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Uncaria tomentosa extract exerts antimicrobial activity against boar seminal bacteria and influences sperm resilience under different conditions

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 21, 2025

Uncaria tomentosa (UT) or cat's claw, is a vine belonging to the Rubiaceae family and native South Central America. Various parts of plant, including bark, showed many therapeutic activities (e.g., antioxidant antibacterial), but in vitro effects on gametes have still not been investigated. During boar semen storage for artificial insemination purposes, oxidative stress bacterial contamination negatively affect sperm quality. In this study, we evaluated tolerance UT ethanolic extract at four concentrations (1.6 0.025 μg/mL). The analyses were carried out samples under stress, induced by H 2 O Fe 2+ /Ascorbate, during 96 h 17°C. antibacterial activity (1,024 8 μg/mL) was tested against commercial strains bacteria isolated from semen. treatments ranging 0.4 μg/mL protected membrane ( p &lt; 0.05) preserved some kinetic parameters (Fe /Ascorbate). storage, did show any cytotoxicity, mean values higher than control group, although significant &gt; 0.05). All Gram-positive exhibited growth inhibition. most frequently Gram-negative (i.e., Citrobacter koseri, Pseudomonas aeruginosa, Stenotrophomonas maltophilia ) also complete inhibition, while remaining partial decrease growth. Taken together, our findings that promising plant-based additive storage.

Language: Английский

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