The Use of Plants Extracts during Cryopreservation of Livestock Epididymal Semen DOI Creative Commons
M. L. Mphaphathi,

Maleke Dimpho Sebopela,

M. R. Ledwaba

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

The plants’ extracts have recently emerged as a cheap and natural source of additives to preserve enhance sperm functions during the cryopreservation process. Extracts from plants are reported be main sources antioxidants with lower cytotoxicity compared therapeutic antioxidants. numerous valuable plant properties, such antioxidants, antimicrobial, antiviral, anti-inflammatory, antiparasitic, been linked extracts. Numerous different species used preservatives for livestock semen cryopreservation. During these processes, added extender in doses keep motile viable throughout cryopreservation, including pre-freezing, equilibration, post-thawing. quality parameters can improved by adding compounds that exert protection against bacteria reactive oxygen scavengers extender. Although application storage is still emerging, their potential promising. They represent economically alternatives conventional currently employed preservation. This review focuses on aspects related role domesticated species.

Language: Английский

Effect of Five Different Antioxidants on the Effectiveness of Goat Semen Cryopreservation DOI
Xiaohua Yi,

Yanbo Qiu,

Xiaoqin Tang

et al.

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(7), P. 1958 - 1972

Published: Jan. 24, 2024

Language: Английский

Citations

8
Antibiotics in semen extenders – a multiplicity of paradoxes DOI
Jane M. Morrell, Aleksandar Cojkić, Pongpreecha Malaluang

et al.

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(5)

Published: March 6, 2024

Addition of antibiotics to semen extenders was taken for granted many years, from the time that commercial artificial insemination in livestock first began decades ago. However, there is now a growing realisation this non-therapeutic utilisation antibacterial agents contrary current recommendations prudent use medical and veterinary professionals are advised follow. Furthermore, not benign, having negative effects on sperm samples, inseminated female, personnel potentially environment. The purpose review three-fold: highlight fact used extenders, with result considerable amounts globally animal breeding, recent studies aspects using purpose, look at possible alternatives. Recent changes legislation regarding occurred some, but all, countries, leaving question marks producers as whether should be added or not.

Language: Английский

Citations

8
Bacterial survival below zero: Impact of storage time on bacterial viability in bull semen DOI Creative Commons
Aleksandar Cojkić, Ingrid Hansson, Jane M. Morrell

et al.

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105233 - 105233

Published: March 19, 2025

Although freezing methods have been optimized for preserving sperm integrity, their effectiveness in sustaining bacterial viability is unknown. Therefore, culturing thawed semen samples might not give an accurate picture of the bacteria original sample. The aim this study was to assess how cryopreservation and storage duration influence populations survival distinct species. Semen were collected from 14 bulls, diluted equal proportions antibiotic-free extender transported laboratory at 6 °C overnight. Aliquots cultured within 24 h after collection on Plate Count Agar calculate number bacteria, blood agar plates (5 % bovine blood) identification remaining 1:1 Brain Heart Infusion (BHI) broth with 30 glycerol stored -80 °C. frozen quantification as described fresh semen, 13 days isolated re-cultured agar, incubated one day 37 before by Matrix assisted laser desorption ionization-time flight mass spectrometry. Total counts remained consistent across cryopreserved regardless duration. A total 31 species identified, 20 detected samples, 16 present storage, 18 observed days. Ten persisted all time points, while others unique a specific sampling day, including nine 1, two 6, five 13. These findings suggest that does alter overall load, individual varies depending conditions.

Language: Английский

Citations

0
Effects of Curcumin Oil Nano‐Emulsion on Buffalo Sperm Cryopreservation: Insights on Sperm Quality, Kinematic Parameters, Sperm Ultrastructure, Oxidative Stress, Apoptosis, Microbiota, and Molecular Docking Analysis and Fertilising Capacity DOI
Wael A. Khalil, Mahmoud A. E. Hassan, Mohamed M. Hegazy

et al.

Reproduction in Domestic Animals, Journal Year: 2025, Volume and Issue: 60(4)

Published: April 1, 2025

ABSTRACT This study investigated the potential of incorporating curcumin oil nano‐emulsion (CONE) into a Tris‐based freezing extender to relieve cryodamage effects and enhance sperm cryo‐resistance in buffalo bulls. Pooled semen from five fertile bulls ( n = 40 ejaculates) was divided supplemented with 0.5% CONE (CONE0.5), 1% (CONE1), or without (CONE0, control), then extended packaged. Post‐thaw assessments included kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, conception rates. The CONE0.5 group exhibited significantly higher viability, progressive motility, membrane as well lower nitric oxide MDA compared CONE0 CONE1 groups p < 0.05), indicating reduced stress. Compared group, percentage viable early apoptotic sperm, late 0.05). Both CONE‐treated demonstrated post‐thaw total bacterial, spore‐forming coliform bacterial counts Conception rates were (80.0%) (68.0%) > Molecular analysis revealed strong binding affinities between caspase‐3 (−7.87 kcal/mol), HSP70 (−8.52 PRDX‐1 (−8.90 suggesting mechanisms action. In summary, inclusion cryoprotective additive media demonstrates for improving quality possible enhancement reproductive efficiency.

Language: Английский

Citations

0
Use of bull sperm extenders: current status (review) DOI Creative Commons
D. A. Starikova, Olga Y. Barkova, И.В. Чистякова

et al.

Agricultural science Euro-North-East, Journal Year: 2025, Volume and Issue: 26(2), P. 223 - 238

Published: April 25, 2025

In recent years, assisted reproductive technologies in animal production have included artificial insemination need of high-quality cryopreserved bull sperm. High-quality freezing depends on the optimally selected parameters semen diluent. this connection, purpose review is to spread knowledge about use various types diluents and additives them cryopreservation improve its quality. This article provides an overview 64 scientific papers domestic foreign researchers modeling order ensure maximum yield sperm after thawing. Research into creation optimal diluent protection save from cryoinjury during a long time, which can preserve properties liquid nitrogen has been carried out since mid-20th century. Classic media with known composition (LPG, Tris, MOPS, etc.) commercial (Andromed, BullXcell, OptiXcell, are used dilute before cryopreservation. The different variants extenders by countries important information effect extender compositions quality parameters, such as motility, viability, percentage abnormal sperm, etc., long-term Also, recently, inclusion natural or synthetic origin extenders, exhibit effects spermatozoa, widely used. may be useful for organizations laboratories engaged development creating extenders.

Language: Английский

Citations

0
Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation DOI Creative Commons
Hao Lin, Zhang Hu, Yang Li

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 441 - 441

Published: May 5, 2025

This study aimed to analyze the effect of curcumin on antioxidant properties and fertility freeze–thawed bovine spermatozoa oocytes. In this study, concentrations 0, 5, 10, 25, 50 µM were added sperm cryopreservation solution oocyte IVM medium assess quality, properties, maturation, IVF rate, embryonic development. The results demonstrated that adding significantly improved viability, motility, acrosome integrity bull after freezing thawing (p < 0.05). addition 25 resulted in best quality. Analysis capacity showed increased activities MMP enzymes, such as CAT, SOD, GSH-PX, lowered levels MDA ROS Adding vitro maturation notably enhanced rate decreased DNA fragmented nuclei oocytes 0.05), with optimal outcomes observed at curcumin. Totals markedly elevated GSH reduced malondialdehyde fertilization rates blastocyst formation conclusion, incorporating into both semen quality frozen–thawed sperm, activity,

Language: Английский

Citations

0
Single layer centrifugation as a method for bacterial reduction in bull semen for assisted reproduction DOI Creative Commons
Aleksandar Cojkić, Ingrid Hansson, Anders Johannisson

et al.

Veterinary Research Communications, Journal Year: 2023, Volume and Issue: 48(1), P. 39 - 48

Published: July 22, 2023

Semen samples contain bacteria originating from the animal urogenital tract, environment, and/or contamination during semen processing, negatively affecting sperm quality by producing toxins competing for nutrients in extenders. The aims of this study were to evaluate two methods Single-layer centrifuges (SLC), high and low density colloid, as a method bacterial removal bull semen, after treatment. In total, 20 bulls (3 ejaculates per bull) used study. Bacterial reduction was evaluated quantification (colony forming unit - CFU/mL) while identification performed matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) culturing on blood agar. Sperm motility parameters Computer Assisted Analyses (CASA), chromatin structure assay (SCSA) Flow cytometry. Both, High Low SLC reduced number significantly (p < 0.001) compared with control. difference count between also significant 0.001). Furthermore, successful removing almost all Bacillus Proteus spp. Most CASA improved both treatments 0.001, p 0.01, 0.05). Deoxyribonucleic acid (DNA) fragmentation index SCSA 0.01) 0.05) group differed centrifugation (SLC) either or colloid is suitable semen.

Language: Английский

Citations

6
Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives DOI Creative Commons
Michal Ďuračka, Filip Benko, Milan Chňapek

et al.

Sensors, Journal Year: 2023, Volume and Issue: 23(15), P. 6978 - 6978

Published: Aug. 6, 2023

The primary role of semen processing and preservation is to maintain a high proportion structurally functionally competent mature spermatozoa, that may be used for the purposes artificial reproduction when needed, whilst minimizing any potential causes sperm deterioration during ex vivo handling. Out multitude variables determining success preservation, bacterial contamination has been acknowledged with an increased interest because its often unpredictable complex effects on quality. Whilst antibiotics are usually most straight-forward option prevent semen, antimicrobial resistance become serious threat requiring widespread attention. As such, besides discussing consequences bacteriospermia vitality risks antibiotic overuse in andrology, this paper summarizes currently available evidence alternative strategies prior to, during, following processing, selection, preservation. Alternative antibacterial supplements reviewed, emphasis given modern methods selection combined by physical removal bacteria or use assisted reproductive technologies.

Language: Английский

Citations

5
Metagenomic identification of bull semen microbiota in different seasons DOI Creative Commons
Aleksandar Cojkić, Adnan Niazi, Jane M. Morrell

et al.

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 268, P. 107569 - 107569

Published: July 30, 2024

A seasonal effect on sperm quality parameters was observed previously. Although identification of the bull semen microbiota by 16S rRNA sequencing performed previously, it has not been carried out in commercial samples from different seasons, and its connection with evaluated yet. The objectives this study were; (i) to evaluate diversity (ii) find if specific bacteria were associated differences parameters. Bull identified 54 3 seasons (winter, spring, summer). Sperm analysed Computer Assisted Analyses (CASA) Flow Cytometry (FC). From 28 phyla all samples, six their distribution. At genus level, 388 genera identified, which 22 had a relative abundance over 1 % showed bacterial diversity, 9 present seasons. Differences between spring summer (P < 0.05) for live hydrogen peroxide positive cells. trend towards significance (0.10 > P some CASA kinematics (VCL LIN) FC (High respiratory activity, cells) Nevertheless, associations spring.

Language: Английский

Citations

0
Comparison of two methods of extracting bull epididymal spermatozoa DOI Creative Commons
Ziyad Al‐Kass,

Sanna Eriksson,

Jaana Peippo

et al.

Veterinary and Animal Science, Journal Year: 2024, Volume and Issue: 26, P. 100407 - 100407

Published: Nov. 7, 2024

Extraction of epididymal spermatozoa may be necessary to avoid losing valuable genetic material, for example, from individuals rare breeds or endangered species, but the resulting sperm samples poor quality. Two methods extracting bull slaughterhouse material were compared. The bulls 16-23 months age. Spermatozoa extracted by making an incision one cm in length tail epididymis allow flow out (method A), flushing B). two used each bull, alternating between right and left epididymis, i.e. if method A was Bull 1, it 2, etc. Sperm concentration adjusted 69 × 10

Language: Английский

Citations

0