Redox Homeostasis and Regulation in Pluripotent Stem Cells: Uniqueness or Versatility? DOI Open Access
Julia Ivanova, O. G. Lyublinskaya

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(20), P. 10946 - 10946

Published: Oct. 11, 2021

Pluripotent stem cells (PSCs) hold great potential both in studies on developmental biology and clinical practice. Mitochondrial metabolism that encompasses pathways generate ATP produce ROS significantly differs between PSCs somatic cells. Correspondingly, for quite a long time it was believed the redox homeostasis is also highly specific due to hypoxic niche of their origin—within pre-implantation blastocyst. However, recent research showed parameters cultivated have much common with differentiated progeny Moreover, has been proven that, similar cells, maintaining physiological level critical regulation PSC identity, proliferation, differentiation, de-differentiation. In this review, we aimed summarize signaling compare profiles pluripotent We collected evidence possess metabolic plasticity are able adapt hypoxia normoxia, pluripotency not strictly associated anaerobic conditions, cellular many other under vitro conditions may be explained by high conservatism system.

Language: Английский

The pentose phosphate pathway in health and disease DOI Open Access
Tara TeSlaa, Markus Ralser, Jing Fan

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(8), P. 1275 - 1289

Published: Aug. 23, 2023

Language: Английский

Citations

186
Overview of Cancer Metabolism and Signaling Transduction DOI Open Access
Heesuk Chae, Seong‐Tshool Hong

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 12 - 12

Published: Dec. 20, 2022

Despite the remarkable progress in cancer treatment up to now, we are still far from conquering disease. The most substantial change after malignant transformation of normal cells into is alteration their metabolism. Cancer reprogram metabolism support elevated energy demand as well acquisition and maintenance malignancy, even nutrient-poor environments. metabolic alterations, under aerobic conditions, such upregulation glucose uptake glycolysis (the Warburg effect), increase ROS (reactive oxygen species) glutamine dependence, which prominent features Among these high dependency has attracted serious attention research community. In addition, oncogenic signaling pathways well-known important genetic mutations play regulatory roles, either directly or indirectly, central carbon identification convergent phenotypes crucial targeting cells. this review, investigate relationship between signal transduction pathways, highlight recent developments anti-cancer therapy that target

Language: Английский

Citations

40
Redox-regulating nanotherapeutics potentiating ferroptosis-driven cancer treatment DOI
Xiaohong Yang, Xinxin Sun, Jin Lei

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 484, P. 149518 - 149518

Published: Feb. 10, 2024

Language: Английский

Citations

11
Signaling Pathways in Oxidative Stress-Induced Neurodegenerative Diseases: A Review of Phytochemical Therapeutic Interventions DOI Creative Commons

Zahra Sebghatollahi,

Ruchika Yogesh, Neelima Mahato

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 457 - 457

Published: April 12, 2025

Oxidative stress, a pivotal driver of neurodegenerative diseases, results from an imbalance between the generation reactive oxygen species (ROS) and cellular antioxidant defenses. This review provides comprehensive analysis key oxidative stress sources, focusing on NADPH oxidase (NOX) hyperactivity mitochondrial Uncoupling Protein (UCP) downregulation. Critically, we examine therapeutic potential phytochemicals in mitigating NOX-mediated ROS through direct enzyme inhibition, including impacts NOX subunit assembly gene expression. Furthermore, explore ability to bolster defenses by activating Kelch-like ECH-associated protein 1 (KEAP1)/nuclear factor erythroid 2-related 2 (Nrf2)/antioxidant response element (ARE) signaling pathway, elucidating upregulation genes, such as GPx, SOD, CAT, HO-1. expands beyond confined overviews; emphasizes specific molecular interactions target proteins, isoforms; in-depth genes upregulated via Nrf2. approach aims pave way for targeted translatable strategies diseases. Ultimately, this illuminates intricate dynamics diseases; underscores restore redox homeostasis reverse pathological conditions precise modulation pathways.

Language: Английский

Citations

1
Acute Myeloid Leukemia Cells Educate Mesenchymal Stromal Cells toward an Adipogenic Differentiation Propensity with Leukemia Promotion Capabilities DOI
Luwen Zhang, Qiong Zhao, Cang Hui

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(16)

Published: March 20, 2022

Mesenchymal stromal cells (MSCs) are essential elements of the bone marrow (BM) microenvironment, which have been widely implicated in pathways that contribute to leukemia growth and resistance. Recent reports showed genotypic phenotypic alterations patient-derived MSCs, indicating MSCs might be educated/reprogrammed. However, results inconclusive, possibly due heterogeneity leukemia. Here, authors report acute myeloid (AML) induces towards an adipogenic differentiation propensity. RNAseq analysis reveal significant upregulation gene expression enriched adipocyte process reduction osteoblast differentiation. The alteration is accompanied by a metabolic switch from glycolysis more oxidative phosphorylation-dependent manner. Mechanistic studies identify AML cell-derived exosomes play vital role during cell-mediated education/reprogramming process. Pre-administration mice BM microenvironment with AML-derived greatly enhance engraftment vivo. quantitative proteomic identified list exosomal protein components differently expressed exosomes, represent opportunity for novel therapeutic strategies based on targeting exosome-based cells-MSCs communication. Collectively, data show AML-educated tend differentiate into adipocytes contributing disease progression, suggests complex interactions components.

Language: Английский

Citations

35
Plasma–liquid interactions in the presence of organic matter—A perspective DOI
Katharina Stapelmann, Sophia Gershman, Vandana Miller

et al.

Journal of Applied Physics, Journal Year: 2024, Volume and Issue: 135(16)

Published: April 26, 2024

As investigations in the biomedical applications of plasma advance, a demand for describing safe and efficacious delivery is emerging. It quite clear that not all plasmas are "equal" applications. This Perspective discusses limitations existing parameters used to define context need "right plasma" at dose" each "disease system." The validity results extrapolated from

Language: Английский

Citations

8
Discovery of Novel Aldose Reductase Inhibitors via the Integration of Ligand-Based and Structure-Based Virtual Screening with Experimental Validation DOI Creative Commons
Muhammad Yasir, Jin‐Young Park, Wanjoo Chun

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(18), P. 20338 - 20349

Published: April 23, 2024

Aldose reductase plays a central role in diabetes mellitus (DM) associated complications by converting glucose to sorbitol, resulting harmful increase of reactive oxygen species (ROS) various tissues, such as the heart, vasculature, neurons, eyes, and kidneys. We employed comprehensive approach, integrating both ligand- structure-based virtual screening followed experimental validation. Initially, candidate compounds were extracted from extensive drug chemical libraries using DeepChem's GraphConvMol algorithm, leveraging its capacity for robust molecular feature representation. Subsequent refinement docking dynamics (MD) simulations, which are crucial understanding compound–receptor interactions dynamic behavior simulated physiological environment. Finally, subjected validation their biological activity an aldose inhibitor kit. The approach led identification promising compound, demonstrating significant potential inhibitor. This not only yields therapeutic intervention DM-related but also establishes integrated protocol development, setting new benchmark field.

Language: Английский

Citations

6
Targeting the redox system for cardiovascular regeneration in aging DOI Creative Commons
M Allemann,

Pratintip Lee,

Jürg H. Beer

et al.

Aging Cell, Journal Year: 2023, Volume and Issue: 22(12)

Published: Nov. 13, 2023

Abstract Cardiovascular aging presents a formidable challenge, as the process can lead to reduced cardiac function and heightened susceptibility cardiovascular diseases. Consequently, there is an escalating, unmet medical need for innovative effective regeneration strategies aimed at restoring rejuvenating tissues. Altered redox homeostasis accumulation of oxidative damage play pivotal role in detrimental changes stem cell cellular senescence, hampering regenerative capacity aged system. A mounting body evidence underscores significance targeting machinery restore self‐renewal enhance their differentiation potential into youthful lineages. Hence, holds promise target optimizing therapies. In this context, we delve current understanding regulating reprogramming processes that impact Furthermore, offer insights recent translational clinical implications redox‐targeting compounds enhancing therapies

Language: Английский

Citations

14
Cystathionine gamma-lyase (CTH) inhibition attenuates glioblastoma formation DOI Creative Commons

Maria Peleli,

Ivi Antoniadou, Dorival Mendes Rodrigues‐Junior

et al.

Redox Biology, Journal Year: 2023, Volume and Issue: 64, P. 102773 - 102773

Published: June 5, 2023

Glioblastoma (GBM) is the most common type of adult brain tumor with extremely poor survival. Cystathionine-gamma lyase (CTH) one main Hydrogen Sulfide (H2S) producing enzymes and its expression contributes to tumorigenesis angiogenesis but role in glioblastoma development remains poorly understood. Principal Results: An established allogenic immunocompetent vivo GBM model was used C57BL/6J WT CTH KO mice where volume microvessel density were blindly measured by stereological analysis. Tumor macrophage stemness markers blinded immunohistochemistry. Mouse human cell lines for cell-based analyses. In gliomas, analyzed bioinformatic analysis on different databases. vivo, genetic ablation host led a significant reduction protumorigenic transcription factor sex determining region Y-box 2 (SOX2). The (indicative angiogenesis) levels peritumoral macrophages showed no changes between two genotypes. Bioinformatic glioma tumors revealed that higher positively correlated SOX2 associated worse overall survival all grades gliomas. Patients not responding temozolomide have also expression. mouse or cells, pharmacological inhibition (PAG) knockdown (siRNA) attenuates proliferation, migration stem formation frequency. Inhibition could be new promising target against formation.

Language: Английский

Citations

11
NeuroD4 converts glioblastoma cells into neuron-like cells through the SLC7A11-GSH-GPX4 antioxidant axis DOI Creative Commons
Hao Wang, Peiqi Zhao, Ying Zhang

et al.

Cell Death Discovery, Journal Year: 2023, Volume and Issue: 9(1)

Published: Aug. 15, 2023

Cell fate and proliferation ability can be transformed through reprogramming technology. Reprogramming glioblastoma cells into neuron-like holds great promise for treatment, as it induces their terminal differentiation. NeuroD4 (Neuronal Differentiation 4) is a crucial transcription factor in neuronal development has the potential to convert astrocytes functional neurons. In this study, we exclusively employed reprogram cells. vivo, reprogrammed demonstrated differentiation, inhibited proliferation, exited cell cycle. Additionally, virus-infected xenografts exhibited smaller sizes compared GFP group, tumor-bearing mice GFP+NeuroD4 group experienced prolonged survival. Mechanistically, overexpression significantly reduced expression of SLC7A11 Glutathione peroxidase 4 (GPX4). The ferroptosis inhibitor ferrostatin-1 effectively blocked NeuroD4-mediated process neuron glioblastoma. To summarize, our study demonstrates that SLC7A11-GSH-GPX4 signaling pathway, thus offering novel therapeutic approach

Language: Английский

Citations

11