AMELIORATING POST-THAWED SEMEN OF BUFFALO BULLS USING A MILK-BASED EXTENDER SUPPLEMENTED WITH RESVERATROL

IRAQI JOURNAL OF AGRICULTURAL SCIENCES, Journal Year: 2024, Volume and Issue: 55(Special), P. 186 - 194

Published: Jan. 25, 2024

The objective of the current study was to investigate influence adding resveratrol (Res) a milk-based extender on some post-thawed semen quality Iraqi buffalo bulls for different preservation periods (cooling at 5˚C, 48 h, 1, and 2 months post-cryopreservation; PC). Four bulls, 3.5 4 years old, were used from 11th November 2020 1st June 2021. Semen samples collected, pooled, divided into four groups using Res concentrations (0, 100, 150, 200 μM) added milk extender. Res-150 μM group exhibited higher (P≤0.01) plasma membrane, acrosome integrity percentages, total antioxidants concentration (TAC) compared other groups. Sperm abnormalities DNA damage as well malondialdehyde (MDA) concentration, lesser in group. Finally, increased percentage live sperm TAC seminal minimized abnormal percentage, MDA bulls.

Language: Английский

---

“Fine feathers make fine birds” – The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction

Animal Reproduction Science, Journal Year: 2021, Volume and Issue: 246, P. 106884 - 106884

Published: Nov. 5, 2021

The sperm plasma membrane is important in modulating many functions. composed of a complex mixture lipids including phospholipids, glycolipids and sterols. There are differences composition among mammalian species with two groups differing the most abundant polyunsaturated fatty acid (PUFA), either docosahexaenoic (ω-3 PUFA) or docosapentaenoic (ω-6 PUFA). During testicular epididymal maturation, evolves spermatozoa gaining capacity for fertilization. importance metabolism complete spermatogenesis has been elucidated using gene knockout mice. transit cholesterol content decreases PUFA increases, conferring more fluidity properties to membrane. relatively lesser antioxidant enzymes larger make particularly susceptible lipid peroxidation during preservation. In numerous studies, there was adding antioxidants diet animals semen extenders aim improve integrity. This review highlights current knowledge on effects function domestic animals.

Language: Английский

---

Role of Antioxidants of Natural Herbs in Management of Male Infertility

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2023, Volume and Issue: 2(1), P. 55 - 80

Published: Feb. 5, 2023

If you and your spouse have been trying to conceive for a year neither of has successful, there is possibility that are one the 50–80 million people throughout world who struggle with infertility. There male component present in approximately 20%-30% these instances. It common knowledge low-quality sperm oxidative stress (OS) both role aetiology Because oxidation DNA, proteins, lipids, excessive levels reactive oxygen species (ROS) potential disrupt viability, motility, morphology cells. ROS responsible changes. Methods: Through use PubMed interface, we searched MEDLINE database studies had completed published during past ten years analysed effect antioxidants on infertile guys (2012–2022). A number different phrases, including infertility, alpha-lipoic acid, zinc, folate, coenzyme Q10, selenium, vitamin, were utilised search. The study's findings indicate inositol serves purpose mechanisms foster contacts between oocytes, it also affects OS cells by way its engagement mitochondrial events. Alpha-lipoic acid (ALA) lessens damage caused improves quality ways, morphology, count. might be connection not getting enough zinc diet having sperm. Zinc folate two nutrients can boost quantity When taken orally, Q10 increases as well their overall motility forward movement. therapy selenium (Se) results an improvement sperm's quality, this connected increase ejaculatory production. Only vitamin B12 found improve sperm; does boosting count decreasing DNA damage. Vitamin only proved do this. Conclusions: Dietary supplementation may men semen reducing OS-induced increasing hormone synthesis spermatozoa concentration, morphology. This achieved low semen. Antioxidants exert effects variety ways; hence, important researchers conducting future clinical trials study combining antioxidants.

Language: Английский

---

Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance

Antioxidants, Journal Year: 2022, Volume and Issue: 11(2), P. 417 - 417

Published: Feb. 18, 2022

This study investigated whether the activities of antioxidant components donkey seminal plasma (SP)-both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) non-enzymatic (measured in terms total thiol, copper-reducing capacity (CUPRAC), ferric-reducing ability (FRAP), Trolox equivalent (TEAC))-and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per individual) were collected split into two aliquots. The first one was used for measuring levels antioxidants OSI SP, whereas other aliquot cryopreserved. Before cryopreservation, quality parameters (concentration, motility, viability) evaluated. After thawing, membrane integrity, lipid disorder, mitochondrial potential, reactive oxygen species (ROS), calcium intracellular also determined. Based on percentages motility (TM) with an intact (SYBR14+/PI-) after samples classified as good-freezability (GFE) or poor-freezability (PFE) through cluster analyses. SP activity (SOD PON1) (CUPRAC, FRAP, TEAC) higher (p < 0.05) GFE than PFE, SP-OSI PFE GFE. In addition, SOD, PON1, GPX, CUPRAC, TEAC positively post-thaw integrity negatively ROS levels. correlated It can thus be concluded that cryotolerance measurements TEAC, well SP-OSI, could markers Further research addressing relationship these their potential use freezing is warranted.

Language: Английский

---

Fluid preservation in brain banking: a review.

PubMed, Journal Year: 2024, Volume and Issue: 5

Published: Jan. 1, 2024

Fluid preservation is nearly universally used in brain banking to store fixed tissue specimens for future research applications. However, the effects of long-term immersion on neural circuitry and biomolecules are not well characterized. As a result, there need synthesize studies investigating fluid tissue. We searched PubMed other databases identify measuring nervous system categorized based preservative used: formaldehyde solutions, buffer alcohol storage after clearing, cryoprotectant solutions. identified 91 containing 197 independent observations cellular morphology. Most did report any significant alterations due storage. When present, most frequent alteration was decreased antigenicity, commonly attributed progressive crosslinking by aldehydes that renders increasingly inaccessible over time. To build mechanistic understanding, we discuss biochemical aspects preservation. A subset lipids appears be chemical altered or extracted time incomplete retention crosslinked gel. Alternative fluids mitigate problem antigen masking but have been extensively characterized may downsides. also compare cryopreservation, paraffin embedding, resin embedding. Overall, existing evidence suggests provides maintenance architecture decades, including precise structural details. avoid well-established overfixation caused high concentration procedures can use an initial fixation step followed alternative fluid. Further warranted optimizing protocols characterizing generalizability artifacts identified.

Language: Английский

---

In vitro study of resveratrol as an antioxidant for boar semen preservation: A systematic review

Veterinary World, Journal Year: 2025, Volume and Issue: unknown, P. 85 - 94

Published: Jan. 1, 2025

In the global pig farming industry, artificial insemination (AI) is widely used to propagate high-quality genetics using processed semen that can be stored for extended periods. However, boar sperm are particularly susceptible oxidative damage during storage and cryopreservation because of their high polyunsaturated fatty acid content. This study aimed systematically review impact resveratrol (RVT), a potent antioxidant, on preservation semen. A comprehensive meta-analysis was conducted following Preferred Reporting Items Systematic Reviews Meta-Analyses guidelines. Relevant articles were retrieved from databases such as Scopus, ScienceDirect, PubMed specific keywords. After thorough screening, 10 studies selected inclusion. Data extracted these included parameters motility, viability, DNA integrity, lipid peroxidation levels. Resveratrol supplementation in extenders significantly improved quality under various conditions, including waterbath cooled frozen RVT's antioxidative properties effectively reduced reactive oxygen species prevented stress-related cells. The addition enhances by mitigating damage, potentially increasing success rate AI swine industry. highlights need further research optimize RVT dosage application methods different techniques.

Language: Английский

---

Storage Conditions of Sperm Samples and Gametic Characterization by Sperm Head Morphometry in Drones (Apis mellifera)

Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 672 - 672

Published: Feb. 26, 2025

The present study aimed to evaluate an optimal method transport and store drone sperm samples, as well characterize spermatozoa through head morphometry. A total of 291 mature drones were used. We performed three experiments. In a first experiment, variables assessed under different incubation conditions (5 °C, 15 37 °C with 5% CO2). Results showed that viability was optimally maintained at (p ˂ 0.05). the second supplementation extender catalase (200 UI) improved 0.05) motility during liquid storage times. Finally, morphometric analysis made: length 5.13 µm, width 0.85 area 3.78 µm2, perimeter 15.01 acrosome 3.50 µm. variability in morphometry calculated by coefficients variation (CV) within- between-drones. CV within-drone higher than between-drones for all parameters regardless hive origin, indicating high degree pleomorphism.

Language: Английский

---

Supplementation of DHA enhances the cryopreservation of yak semen via alleviating oxidative stress and inhibiting apoptosis

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 26, 2025

Semen cryopreservation is a crucial method for preserving genetic resources and accelerating the breeding process in domestic animals. However, frozen-thawed often leads to physical chemical damage semen, resulting oxidative stress that diminishes sperm vitality fertilization potential. This study aimed explore effects of docosahexaenoic acid (DHA) on quality yak semen. samples were collected from six healthy adult Maiwa yaks cryopreserved liquid nitrogen using extenders with varying DHA concentrations: 0, 0.1, 1, 10, 100 ng/mL. After thawing, we assessed indices, antioxidant capacity, mitochondrial activity, apoptosis status identify optimal concentration. Our findings indicate addition significantly improved total motility (TM), progressive (PM), velocity straight line (VSL), curvilinear (VCL), average path (VAP) spermatozoa, as well integrity membrane acrosome (P < 0.05). Additionally, supplementation markedly reduced levels reactive oxygen species (ROS) malondialdehyde (MDA) spermatozoa 0.05) enhanced enzyme activities (T-AOC, SOD, CAT, GSH-Px, P It also potential (MMP) ATP Notably, group treated 10 ng/mL showed better outcomes than other treatment groups Furthermore, semen dilution effectively decreased apoptotic ratio 0.05), notably upregulated expression level anti-apoptotic protein Bcl-2 while downregulating pro-apoptotic Bax Caspase3 In conclusion, incorporation into enhances viability after by alleviating stress, bolstering defenses, mitochondria function, inhibiting pathway activation.

Language: Английский

---

Polydopamine-based nano-protectant for prolonged boar semen preservation by eliminating ROS and regulating protein phosphorylation via D2DR-mediated cAMP/PKA signaling pathway

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 27, 2025

Language: Английский

---

Optimization of rooster semen preservation: a comparative study of extender types and antioxidant supplementation strategies during cold storage

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105137 - 105137

Published: April 1, 2025

This study evaluated the effect of semen extenders and antioxidant supplementation strategies on quality fertility Thai native rooster during cold storage. In Experiment 1, four (EK, IGGKPh, NaCl, NCAB) were for their ability to preserve sperm motility, viability, lipid peroxidation (MDA), oxidative stress (reactive oxygen species; ROS) over 24 h at 5°C. EK IGGKPh yielded superior motility (68.72% 70.27%) viability (91.96% 91.97%) while minimizing MDA (0.93 1.12 µmol) ROS (14.44% 14.04%) compared NaCl (motility: 53.14%, viability: 82.09±3.15, MDA: 1.59 µmol; P < 0.01). 2 examined effects dietary selenium (0.6 ppm), extender-based glutathione (GSH) (2mM), combination across all extenders. Antioxidant treatments, particularly GSH its with selenium, improved stability (P 0.05). At h, GSH-enriched groups using exhibited enhanced (67.90% 71.49%) controls (57.97% 55.09%). group, combined treatments significantly 12 (50.83% 50.01%, respectively) control (40.80%; Selenium also increased concentration (4.24 ± 0.55 × 10⁹ vs. 2.46 0.22 spz/mL; 3 outcomes extender same from 2. Fertility rates higher in (70.38%) (69.16%) than (54.26%; 0.01), hatchability remained unaffected > 0.05).In summary, composition strategy has a significant influence fertility. The extenders, especially when supplemented GSH, offered protection, combining synergistic benefits preservation reproductive success.

Language: Английский

---