Oxidative Stress, Cytotoxic and Inflammatory Effects of Azoles Combinatorial Mixtures in Sertoli TM4 Cells DOI Creative Commons
Sabrina Petricca, Veronica Carnicelli,

Carla Luzi

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(6), P. 1142 - 1142

Published: May 24, 2023

Triazole and imidazole fungicides are an emerging class of contaminants with increasing ubiquitous presence in the environment. In mammals, their reproductive toxicity has been reported. Concerning male reproduction, a combinatorial activity tebuconazole (TEB; triazole fungicide) econazole (ECO; compound) inducing mitochondrial impairment, energy depletion, cell cycle arrest, sequential activation autophagy apoptosis Sertoli TM4 cells (SCs) recently demonstrated. Given strict relationship between reactive oxygen species (ROS), causative role oxidative stress (OS) dysfunction, individual combined potential TEB ECO redox status alterations OS was investigated. Furthermore, considering impact cyclooxygenase (COX)-2 tumor necrosis factor-alpha (TNF-α) modulating fertility, protein expression levels were assessed. present study, we demonstrate that azoles-induced cytotoxicity is associated significant increase ROS production, drastic reduction superoxide dismutase (SOD) GSH-S-transferase levels, marked oxidized (GSSG) glutathione. Exposure to azoles also induced COX-2 increased TNF-α production. pre-treatment N-acetylcysteine (NAC) mitigates accumulation, attenuates rescues SCs from azole-induced apoptosis, suggesting ROS-dependent molecular mechanism underlying cytotoxicity.

Language: Английский

Mitochondrial function and reactive oxygen species production during human sperm capacitation: Unraveling key players DOI
Pilar Irigoyen, Santiago Mansilla, Laura Castro

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(4)

Published: Feb. 26, 2024

Abstract Sperm capacitation is a critical process for male fertility. It involves series of biochemical and physiological changes that occur in the female reproductive tract, rendering sperm competent successful fertilization. The precise mechanisms and, specifically, role mitochondria, remain incompletely understood. Previously, we revealed mouse mitochondrial activity (e.g., oxygen consumption, membrane potential, ATP/ADP exchange, Ca 2+ ) increases during capacitation. Herein, studied function by high‐resolution respirometry (HRR) reactive species production capacitated (CAP) non‐capacitated (NC) human spermatozoa. We found from normozoospermic donors, respiratory control ratio increased 36%, accompanied double consumption rate (OCR) presence antimycin A. Extracellular hydrogen peroxide (H 2 O detection was three times higher CAP than NC cells. To confirm H depends on superoxide () formation, evaluated aconitase (ACO2) amount, activity, metabolic flux tricarboxylic acid cycle. estimated cells produce, average individual, (59 ± 22)% more steady‐state compared to Finally, analyzed two targets oxidative stress: lipid peroxidation western blot against 4‐hydroxynonenal succinate dehydrogenase (SDH) HRR. did not observe modifications lipoperoxidation nor SDH, suggesting capacitation, increase does damage it necessary normal process.

Language: Английский

Citations

9
Impact of Ultraviolet C Radiation on Male Fertility in Rats: Suppression of Autophagy, Stimulation of Gonadotropin-Inhibiting Hormone, and Alteration of miRNAs DOI Open Access
Ahmed Mohamed Alahwany, Ahmed Hamed Arisha,

Adel Abdelkhalek

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 316 - 316

Published: Jan. 1, 2025

While ultraviolet C (UVC) radiation has beneficial applications, it can also pose risks to living organisms. Nevertheless, a detailed assessment of UVC radiation’s effects on mammalian male reproductive physiology, including the underlying mechanisms and potential protective strategies, not yet been accomplished. This study aimed examine critical roles oxidative stress, autophagy, hormonal axis, microRNAs in UVC-induced challenges rats. Semen, biochemical, molecular, silico analyses revealed significant dysregulation testicular steroidogenesis, impaired spermatogenesis, deteriorated sperm quality, altered profiles, which ultimately lead decline fertility rats exposed radiation. Our data indicated that suppression stimulation gonadotropin-inhibiting hormone (GnIH), alteration serve as key mediators stress reproduction, potentially contributing infertility. Targeting these pathways, particularly through pretreatment with hesperidin (HES), offers promising strategy counteract In conclusion, present findings emphasize importance understanding molecular behind infertility offer valuable insights into prospective role HES safeguarding health.

Language: Английский

Citations

1
Decoding the Puzzle of Male Infertility: The Role of Infection, Inflammation, and Autoimmunity DOI Creative Commons
Romualdo Sciorio, Lina De Paola, Tiziana Notari

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(5), P. 547 - 547

Published: Feb. 24, 2025

Background/Objectives: Male infertility is a complex, multifactorial condition influenced by infectious, inflammatory, and autoimmune components. Immunological factors, though implicated in reproduction, remain poorly understood. This study aims to deepen the understanding of infections, inflammation, factors male infertility, with focus on immune-related disorders affecting testes epididymis-immunologically privileged but vulnerable sites. These can impair sperm quality through oxidative stress (ROS) antisperm antibodies (ASA), further compromising fertility. Methods: A narrative review was conducted analyzing scientific literature from past 10 years PubMed using keywords such as "male infertility", "autoimmunity", "inflammatory disease". Studies focusing testicular epididymal disorders, immunological impacts, therapeutic approaches were included. Results: Our research highlights that conditions like epididymitis, vasectomy, trauma, previous surgeries trigger inflammatory responses, leading ASA formation stress. ASA, particularly sperm-immobilizing antibodies, inhibits motility migration female reproductive tract. Infections caused sexually transmitted bacteria or urinary pathogens frequently induce epididymo-orchitis, primary contributor infertility. While standardized methodologies for testing elusive, assisted treatments intracytoplasmic injection (ICSI), vitro fertilization (IVF), intrauterine insemination (IUI) show promise overcoming immune-mediated Conclusions: underscores critical role infection, responses It necessity improving diagnostic methods, immune-pathological mechanisms, addressing medicolegal issues associated knowledge could pave way innovative therapies, ultimately enhancing fertility outcomes, mitigating societal legal repercussions

Language: Английский

Citations

1
Role of sperm DNA damage in creating de-novo mutations in human offspring: the ‘post-meiotic oocyte collusion’ hypothesis DOI Open Access
R. John Aitken

Reproductive BioMedicine Online, Journal Year: 2022, Volume and Issue: 45(1), P. 109 - 124

Published: March 17, 2022

Language: Английский

Citations

36
Functional association between telomeres, oxidation and mitochondria DOI Creative Commons
Efthalia Moustakli, Athanasios Zikopoulos, Prodromos Sakaloglou

et al.

Frontiers in Reproductive Health, Journal Year: 2023, Volume and Issue: 5

Published: Feb. 20, 2023

Prior research has substantiated the vital role of telomeres in human fertility. Telomeres are prerequisites for maintaining integrity chromosomes by preventing loss genetic material following replication events. Little is known about association between sperm telomere length and mitochondrial capacity involving its structure functions. Mitochondria structurally functionally distinct organelles that located on spermatozoon's midpiece. produce adenosine triphosphate (ATP) through oxidative phosphorylation (OXPHOS), which necessary motility generate reactive oxygen species (ROS). While a moderate concentration ROS critical egg—sperm fusion, fertilization, excessive generation primarily related to shortening, DNA fragmentation, alterations methylation pattern leading male infertility. This review aims highlight functional connection mitochondria biogenesis infertility, as lesions have damaging impact length, both lengthening reprogramming biosynthesis. Furthermore, it shed light how inositol antioxidants can positively affect

Language: Английский

Citations

18
Age-Related Decline of Male Fertility: Mitochondrial Dysfunction and the Antioxidant Interventions DOI Creative Commons
Jingjing Wang, Shuxia Wang,

Tehmina

et al.

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(5), P. 519 - 519

Published: April 23, 2022

Mitochondria are structurally and functionally unique organelles in male gametes. Apparently, as the only remaining mature sperm, mitochondria not produce adeno-sine triphosphate (ATP) through oxidative phosphorylation (OXPHOS) to support sperm mobility, but also play key roles regulating reactive oxidation species (ROS) signaling, calcium homeostasis, steroid hormone biosynthesis, apoptosis. Mitochondrial dysfunction is often associated with aging process. Age-dependent alterations of epididymis can cause mitochondrial functioning. The resultant cellular defects have been implicated infertility. Among these, stress (OS) due overproduction ROS may represent one major causes these disorders. Excessive trigger DNA damage, disturb impair OXPHOS, disrupt integrity lipid membrane, induce Given facts, scavenging by antioxidants hold great potential terms finding promising therapeutic strategies treat Here, we summarize progress made understanding dysfunction, aging, clinical antioxidant interventions was discussed.

Language: Английский

Citations

24
Biomarkers of oxidative stress and reproductive complications DOI
Reinaldo Marı́n, Cilia Abad,

Deliana Rojas

et al.

Advances in clinical chemistry, Journal Year: 2023, Volume and Issue: unknown, P. 157 - 233

Published: Jan. 1, 2023

Language: Английский

Citations

16
From oxidative imbalance to compromised standard sperm parameters: Toxicological aspect of phthalate esters on spermatozoa DOI
Shirsha Mondal, Arindam Bandyopadhyay

Environmental Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 98, P. 104085 - 104085

Published: Feb. 23, 2023

Language: Английский

Citations

16
Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis DOI Creative Commons
Enrico Radaelli, Charles‐Antoine Assenmacher,

Jillian Verrelle

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: July 28, 2023

Impaired spermatogenesis and male infertility are common manifestations associated with mitochondrial diseases, yet the underlying mechanisms linking these conditions remain elusive. In this study, we demonstrate that mice deficient for intra-membrane rhomboid protease PARL, a recently reported model of encephalopathy Leigh syndrome, develop early testicular atrophy caused by complete arrest during meiotic prophase I, followed degeneration death arrested spermatocytes. This process is independent neurodegeneration. Interestingly, genetic modifications PINK1, PGAM5, TTC19 - three major substrates PARL important roles in homeostasis fail to reproduce or modify severe phenotype, indicating spermatogenic arises from distinct molecular pathways. We further observed abnormalities ultrastructure PARL-deficient spermatocytes, along prominent electron transfer chain defects, disrupted coenzyme Q (CoQ) biosynthesis, metabolic rewiring. These defects germ cell-specific decrease GPX4 expression leading spermatocytes ferroptosis regulated cell modality characterized uncontrolled lipid peroxidation. Our results suggest induced depletion act as an initiating trigger primary through simultaneous effects on CoQ two inhibitors ferroptosis. findings shed new light potential role pathogenesis diseases warranting investigation.

Language: Английский

Citations

16
Timeless or tainted? The effects of male ageing on seminal fluid DOI Creative Commons
Claudia Fricke, Krish Sanghvi,

Mischa Emery

et al.

Frontiers in Ecology and Evolution, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 22, 2023

Reproductive ageing can occur due to the deterioration of both soma and germline. In males, it has mostly been studied with respect age-related changes in sperm. However, somatic component ejaculate, seminal fluid, is also essential for maintaining reproductive function. Whilst we know that fluid proteins (SFPs) are required male success across diverse taxa, SFP quantity composition little understood. Additionally, only few studies have explored tissues produce SFPs, resulting outcomes. Here provide a systematic review addressing how advancing age affects production properties particular SFPs oxidative stress, highlighting many open questions generating new hypotheses further research. We additionally discuss declines function different components such as antioxidants, could contribute loss ability. Overall, find evidence results increased stress decrease abundance various SFPs. These suggest contributes towards important influencing reproduction. Thus, study this ignored ejaculate understand ageing, its consequences sexual selection paternal effects on offspring.

Language: Английский

Citations

13