Telomerase reverse transcriptase gene knock‐in unleashes enhanced longevity and accelerated damage repair in mice DOI Creative Commons

Tian‐Yi Zhu,

Po Hu,

Yu‐Hui Mi

et al.

Aging Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

While previous research has demonstrated the therapeutic efficacy of telomerase reverse transcriptase (TERT) overexpression using adeno-associated virus and cytomegalovirus vectors to combat aging, broader implications TERT germline gene editing on mammalian genome, proteomic composition, phenotypes, lifespan extension, damage repair remain largely unexplored. In this study, we elucidate functional properties transgenic mice carrying Tert transgene, guided by precise targeting into Rosa26 locus via embryonic stem (ES) cells under control elongation factor 1α (EF1α) promoter. The knock-in (TertKI) harboring EF1α-Tert displayed elevated activity, elongated telomeres, extended lifespan, with no spontaneous genotoxicity or carcinogenicity. TertKI showed also enhanced wound healing, characterized significantly increased expression Fgf7, Vegf, collagen. Additionally, exhibited robust resistance progression colitis induced dextran sodium sulfate (DSS), accompanied reduced disease-deteriorating genes. These findings foreshadow potential as an extraordinary rejuvenation force, promising not only longevity but in skin intestinal aging.

Language: Английский

The Impact of Follicular Fluid Oxidative Stress Levels on the Outcomes of Assisted Reproductive Therapy DOI Creative Commons
Yu Chen,

Jiahao Yang,

Ling Zhang

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(12), P. 2117 - 2117

Published: Dec. 14, 2023

Oocyte quality is a pivotal determinant of assisted reproductive outcomes. The oocytes intricately linked to their developmental microenvironment, particularly the levels oxidative stress within follicular fluid. Oxidative in fluid may have substantial influence on oocyte health, thereby impacting outcomes ART procedures. This review meticulously explores intricate relationship between and Furthermore, it delves into strategies aimed at ameliorating status fluid, with overarching goal enhancing overall efficacy ART. research endeavors establish robust foundation provide valuable guidance for clinical treatment approaches, context infertile women, including those advanced maternal age.

Language: Английский

Citations

10
Evaluation of telomere length and telomerase activity on predicting in vitro fertilization treatment outcomes DOI

Persefoni Fragkiadaki,

Elisavet Kouvidi,

Anna Angelaki

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2024, Volume and Issue: 41(6), P. 1463 - 1473

Published: May 2, 2024

Language: Английский

Citations

1
Telomeres and SIRT1 as Biomarkers of Gamete Oxidative Stress, Fertility, and Potential IVF Outcome DOI Open Access
Anna Pańczyszyn, Ewa Boniewska‐Bernacka, Iwona Wertel

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8652 - 8652

Published: Aug. 8, 2024

The number of infertile couples undergoing in vitro fertilisation (IVF) has increased significantly. efficacy this procedure is contingent upon a multitude factors, including gamete quality. One factor influencing quality oxidative stress, which leads to telomere damage and accelerates cellular ageing. Identifying new biomarkers that can predict the success assisted reproduction techniques current relevant area research. In review, we discuss potential role SIRT1, protein known protect against stress telomeres, are responsible for genome stability, as technique outcomes.

Language: Английский

Citations

1
Telomerase reverse transcriptase gene knock‐in unleashes enhanced longevity and accelerated damage repair in mice DOI Creative Commons

Tian‐Yi Zhu,

Po Hu,

Yu‐Hui Mi

et al.

Aging Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

While previous research has demonstrated the therapeutic efficacy of telomerase reverse transcriptase (TERT) overexpression using adeno-associated virus and cytomegalovirus vectors to combat aging, broader implications TERT germline gene editing on mammalian genome, proteomic composition, phenotypes, lifespan extension, damage repair remain largely unexplored. In this study, we elucidate functional properties transgenic mice carrying Tert transgene, guided by precise targeting into Rosa26 locus via embryonic stem (ES) cells under control elongation factor 1α (EF1α) promoter. The knock-in (TertKI) harboring EF1α-Tert displayed elevated activity, elongated telomeres, extended lifespan, with no spontaneous genotoxicity or carcinogenicity. TertKI showed also enhanced wound healing, characterized significantly increased expression Fgf7, Vegf, collagen. Additionally, exhibited robust resistance progression colitis induced dextran sodium sulfate (DSS), accompanied reduced disease-deteriorating genes. These findings foreshadow potential as an extraordinary rejuvenation force, promising not only longevity but in skin intestinal aging.

Language: Английский

Citations

1