Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 30, 2024
Abstract
3-Mercaptopyruvate
sulfurtransferase
(3-MST)
is
an
enzyme
that
plays
integral
roles
in
various
biological
processes.
In
the
realm
of
Leishmania,
role
3-MST
less
explored.
It
a
critical
player
maintaining
oxidative
homeostasis
Leishmania
during
stress
for
survival.
This
highlights
potential
Ld3-MST
as
appealing
drug
target.
However,
recognising
structural
disparities
becomes
essential
when
protein
present
host
and
parasite.
study
delves
into
distinctions
between
Hs3-MST,
providing
valuable
insights
with
direct
implications
design.
A
standout
feature
elongated
70
amino
acid
C-terminal
mainly
contributing
to
lid-like
domain
above
active
site
cavity,
setting
it
apart
from
Hs3-MST.
The
RMSD
analysis
shows
fluctuation
due
extended
tail,
while
Rg
SASA
confirm
open
solvent-accessible
nature
Ld3-MST,
especially
its
site,
suggesting
ability
accommodate
larger
molecules.
PC
FEL
reveals
unique
internal
molecular
dynamics
particularly
site.
Docking
studies
demonstrate
Ld3-MST's
can
effectively
molecules,
highlighting
comprehensive
investigation
lays
foundation
developing
precise
inhibitors
promising
therapeutic
applications.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(9), P. 1737 - 1737
Published: Sept. 7, 2023
Hydrogen
sulfide
(H2S),
traditionally
recognized
as
a
toxic
gas,
has
emerged
critical
regulator
in
many
biological
processes,
including
oxidative
stress
and
cellular
homeostasis.
This
review
presents
an
exhaustive
overview
of
the
current
understanding
H2S
its
multifaceted
role
mammalian
functioning
management.
We
delve
into
sources
function
H2S,
mechanisms
underlying
homeostasis,
intricate
relationships
between
these
processes.
explore
evidence
from
recent
experimental
clinical
studies,
unraveling
biochemical
molecular
dictating
H2S’s
roles
modulating
responses
maintaining
The
implications
therapeutic
potential
conditions
characterized
by
dysregulation
disrupted
homeostasis
are
discussed,
highlighting
emerging
significance
health
disease.
Finally,
this
underscores
challenges,
controversies,
future
directions
field,
emphasizing
need
for
further
research
to
harness
agent
diseases
associated
with
homeostatic
imbalance.
Through
review,
we
aim
emphasize
pivotal
function,
encouraging
exploration
burgeoning
area
research.
Non-Coding RNA,
Journal Year:
2024,
Volume and Issue:
10(1), P. 7 - 7
Published: Jan. 18, 2024
Recently,
myriad
studies
have
defined
the
versatile
abilities
of
gasotransmitters
and
their
synthesizing
enzymes
to
play
a
“Maestro”
role
in
orchestrating
several
oncological
non-oncological
circuits
and,
thus,
nominated
them
as
possible
therapeutic
targets.
Although
significant
amount
work
has
been
conducted
on
nitric
oxide
(NO)
carbon
monoxide
(CO)
inter-relationship
field
oncology,
research
about
hydrogen
sulfide
(H2S)
remains
its
infancy.
non-coding
RNAs
(ncRNAs)
reported
dominating
regulation
endogenous
machinery
system
H2S
pathological
contexts.
A
growing
list
microRNAs
(miRNAs)
long
(lncRNAs)
are
leading
way
upstream
regulators
for
biosynthesis
different
mammalian
cells
during
development
progression
human
diseases;
therefore,
targeting
can
be
great
benefit.
In
current
review,
authors
shed
light
onto
biosynthetic
pathways
by
miRNAs
lncRNAs
various
disorders.
Cancer Cell International,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: April 16, 2024
Abstract
Background
Hydrogen
sulfide
(H
2
S)
is
a
significant
endogenous
mediator
that
has
been
implicated
in
the
progression
of
various
forms
cancer
including
breast
(BC).
Cystathionine-β-synthase
(CBS),
cystathionine-γ-lyase
(CSE),
and
3-mercaptopyruvate
sulfurtransferase
(3MST)
are
three
principal
mammalian
enzymes
responsible
for
H
S
production.
Overexpression
CBS,
CSE
3MST
was
found
to
be
associated
with
poor
prognosis
BC
patients.
Moreover,
linked
an
immune-suppressive
tumor
microenvironment
BC.
Recently
it
observed
cells,
response
single
or
dual
inhibition
synthesizing
enzymes,
develop
escape
mechanism
by
overexpressing
alternative
sources
generation.
Thus,
aim
this
work
compensatory
pan
repressing
using
microRNAs
(miRNAs)
investigate
their
impact
on
oncogenic
immunogenic
profile
cells.
Methods
female
patients
(
n
=
25)
were
recruited.
In-silico
analysis
used
identify
miRNAs
targeting
CSE,
3MST.
MDA-MB-231
cells
cultured
transfected
oligonucleotides.
Total
RNA
extracted
Biazol,
reverse
transcribed
quantified
qRT-PCR.
levels
measured
AzMc
assay.
hallmarks
assessed
trans-well
migration,
wound
healing,
MTT,
colony
forming
assays.
Results
miR-193a
miR-548c
validated
eight
different
bioinformatics
software
simultaneously
target
MiR-193a
significantly
downregulated
tissues
compared
non-cancerous
counterparts.
Ectopic
expression
TNBC
resulted
marked
repression
transcript
protein
levels,
decrease
reduction
cellular
viability,
migration
ability,
immune-suppressor
proteins
GAL3
GAL9,
CD155
upregulation
immunostimulatory
MICA
MICB
proteins.
Conclusion
This
study
sheds
light
onto
as
potential
pan-repressors
enzymes.
identifies
them
novel
suppressor
immunomodulatory
TNBC.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(9), P. 1951 - 1951
Published: Aug. 26, 2024
Atherosclerosis
is
a
chronic
inflammatory
condition
marked
by
endothelial
dysfunction,
lipid
accumulation,
cell
infiltration,
and
extracellular
matrix
(ECM)
remodeling
within
arterial
walls,
leading
to
plaque
formation
potential
cardiovascular
events.
Key
players
in
ECM
inflammation
are
metalloproteinases
(MMPs)
CD147/EMMPRIN,
surface
glycoprotein
expressed
on
cells,
vascular
smooth
muscle
cells
(VSMCs),
immune
that
regulates
MMP
activity.
Hydrogen
sulfide
(H₂S),
gaseous
signaling
molecule,
has
emerged
as
significant
modulator
of
these
processes
including
oxidative
stress
mitigation,
reduction,
remodeling.
This
systematic
review
investigates
the
mechanistic
pathways
through
which
H₂S
influences
MMPs
CD147/EMMPRIN
assesses
its
impact
atherosclerosis
progression.
A
comprehensive
literature
search
was
conducted
across
PubMed,
Scopus,
Web
Science
databases,
focusing
studies
examining
modulation
contexts.
Findings
indicate
modulates
expression
activity
transcriptional
regulation
post-translational
modifications,
S-sulfhydration.
By
mitigating
stress,
reduces
activation,
contributing
stability
also
downregulates
via
pathways,
diminishing
responses
cellular
proliferation
plaques.
The
dual
regulatory
role
inhibiting
downregulating
CD147
suggests
therapeutic
agent
stabilizing
atherosclerotic
plaques
inflammation.
Further
research
warranted
elucidate
precise
molecular
mechanisms
explore
H₂S-based
therapies
for
clinical
application
atherosclerosis.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(12), P. 2059 - 2059
Published: Nov. 29, 2023
Taurine
is
a
natural
antioxidant
with
antihypertensive
properties.
Maternal
chronic
kidney
disease
(CKD)
has
an
impact
on
renal
programming
and
increases
the
risk
of
offspring
hypertension
in
later
life.
The
underlying
mechanisms
cover
oxidative
stress,
dysregulated
hydrogen
sulfide
(H2S)
system,
dysbiotic
gut
microbiota,
inappropriate
activation
renin-angiotensin-aldosterone
system
(RAAS).
We
investigated
whether
perinatal
taurine
administration
enables
us
to
prevent
high
blood
pressure
(BP)
complicated
by
maternal
CKD.
Before
mating,
CKD
was
induced
through
feeding
chow
containing
0.5%
adenine
for
3
weeks.
administered
(3%
drinking
water)
during
gestation
lactation.
Four
groups
male
were
used
(n
=
8/group):
controls,
CKD,
taurine-treated
control
rats,
rats
treatment
significantly
reduced
BP
born
mothers
beneficial
effects
attributed
augmented
H2S
pathway,
rebalance
aberrant
RAAS
activation,
microbiota
alterations.
In
summary,
our
results
not
only
deepen
knowledge
CKD-induced
but
also
afford
impetus
consider
taurine-based
intervention
as
promising
preventive
approach
future
clinical
translation.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(4), P. 1741 - 1741
Published: Feb. 18, 2025
Exercise
plays
a
crucial
role
in
maintaining
metabolic
health,
enhancing
muscle
function,
and
improving
insulin
sensitivity,
thereby
preventing
diseases
such
as
type
2
diabetes.
Emerging
evidence
highlights
the
significance
of
cystathionine
γ-lyase
(CSE)/hydrogen
sulfide
(H2S)
signaling
pathway
pivotal
regulator
molecular
physiological
adaptations
induced
by
exercise.
This
review
comprehensively
examines
biosynthesis
metabolism
H2S,
its
distribution
different
tissues,
mechanisms
which
CSE/H2S
influences
contraction,
repair,
protein
synthesis.
Additionally,
it
explores
how
modulates
pathways,
glucose
uptake,
lipid
metabolism,
sensitivity.
The
potential
H2S
donors
exercise
supplements
is
also
discussed,
highlighting
their
ability
to
improve
performance
health.
Current
research
advancements,
including
application
multi-omics
approaches,
are
reviewed
provide
deeper
understanding
complex
networks
involved.
Furthermore,
challenges
future
directions
addressed,
emphasizing
need
for
further
mechanistic
studies
clinical
applications.
underscores
therapeutic
targeting
optimize
benefits
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(5), P. 2237 - 2237
Published: March 2, 2025
I3MT-3
(HMPSNE)
has
been
identified
as
a
selective
inhibitor
of
the
supersulfide-producing
enzyme
3-MST.
In
this
study,
we
found
that
inhibits
inflammatory
responses,
including
secretion
pro-inflammatory
cytokine
interleukin-1β
(IL-1β)
and
cell
death
pyroptosis,
induced
by
activation
inflammasomes
composed
NLRP1,
NLRP3,
or
AIM2.
However,
interestingly,
knockdown
3-MST
did
not
affect
inflammasomes,
suggesting
inhibitory
effect
on
inflammasome
is
mediated
alternative
ways
rather
than
inhibition
Interestingly,
an
in
vitro
caspase
assay
revealed
directly
caspase-1
activation,
molecular
docking
simulations
raised
possibility
pyrimidone
ring
stabilizes
direct
interaction
with
caspase-1.
Taken
together,
our
data
suggest
targeting
caspase-1,
show
potent
