Identification of disulfidptosis-related subtypes, characterization of tumor microenvironment infiltration, and development of a prognosis model in colorectal cancer

Journal of Cancer Research and Clinical Oncology, Journal Year: 2023, Volume and Issue: 149(15), P. 13995 - 14014

Published: Aug. 6, 2023

Language: Английский

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Plasma Concentrations of High Mobility Group Box 1 Proteins and Soluble Receptors for Advanced Glycation End-Products Are Relevant Biomarkers of Cognitive Impairment in Alcohol Use Disorder: A Pilot Study

Toxics, Journal Year: 2024, Volume and Issue: 12(3), P. 190 - 190

Published: Feb. 29, 2024

Alcohol use disorder (AUD) is a major component in the etiology of cognitive decline and dementia. Underlying mechanisms by which long-term alcohol abuse causes dysfunction include excessive oxidative stress inflammation brain, activated increased reactive oxygen/nitrogen species (ROS/RNS), advanced glycation end-products (AGEs) high-mobility group box 1 protein (HMGB1). In pilot study, we examine potential clinical value circulating biomarkers including ROS/RNS, HMGB1, soluble receptor for AGE (sRAGE), brain biomarker aging apolipoprotein D (ApoD), antioxidant regulator nuclear factor erythroid 2-related 2 (NRF2) as predictive indices impairment (CI) abstinent patients with AUD (n = 25) compared to established Alzheimer’s disease (AD, n 26) control subjects 25). Plasma concentrations sRAGE were evaluated immunoblotting; ROS/RNS fluorometric kit; ApoD, NRF2 ELISA. Abstinent had higher sRAGE, (p < 0.05), ApoD 0.01) concentrations, similar those AD patients, lower controls. These changes remarkable CI. correlated positively duration (rho 0.398, p 0.022; rho 0.404, 0.018), whereas negatively periods abstinence −0.340, 0.045). A model duration, was able differentiate CI (92.3% correct predictions, ROC-AUC= 0.90) from without conclusion, propose capable predicting patients.

Language: Английский

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Apolipoprotein D as a Potential Biomarker in Neuropsychiatric Disorders

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15631 - 15631

Published: Oct. 26, 2023

Neuropsychiatric disorders (NDs) are a diverse group of pathologies, including schizophrenia or bipolar disorders, that directly affect the mental and physical health those who suffer from them, with an incidence is increasing worldwide. Most NDs result complex interaction multiple genes environmental factors such as stress traumatic events, recent Coronavirus Disease (COVID-19) pandemic. In addition to clinical presentations, these diseases heterogeneous in their pathogenesis, brain regions affected, symptoms, making diagnosis difficult. Therefore, finding new biomarkers essential for detection, prognosis, response prediction, development treatments NDs. Among most promising candidates apolipoprotein D (Apo D), component lipoproteins implicated lipid metabolism. Evidence suggests increase Apo expression association aging presence neuropathological processes. As part cellular neuroprotective defense machinery against oxidative inflammation, changes levels have been demonstrated neuropsychiatric conditions like (SZ) (BPD), not only some areas but corporal fluids, i.e., blood serum patients. What clear whether variation quantity could be used indicator detect progression. This review aims provide updated view potential possible biomarker

Language: Английский

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Comparison of Commonly Measured Plasma and Cerebrospinal Fluid Proteins and Their Significance for the Characterization of Cognitive Impairment Status

Journal of Alzheimer s Disease, Journal Year: 2023, Volume and Issue: 97(2), P. 621 - 633

Published: Dec. 22, 2023

Background: Although cerebrospinal fluid (CSF) amyloid-β42 peptide (Aβ42) and phosphorylated tau (p-tau) blood p-tau are valuable for differential diagnosis of Alzheimer’s disease (AD) from cognitively normal (CN) there is a lack validated biomarkers mild cognitive impairment (MCI). Objective: This study sought to determine how plasma CSF protein markers compared in the characterization MCI AD status. Methods: cohort included Disease Neuroimaging Initiative (ADNI) participants who had baseline levels 75 proteins measured commonly (257 total, 46 CN, 143 MCI, 68 AD). Logistic regression, least absolute shrinkage selection operator (LASSO) Random Forest (RF) methods were used identify candidates classification. Results: We observed that six panel (APOE, AMBP, C3, IL16, IGFBP2, APOD) outperformed seven (VEGFA, HGF, PRL, FABP3, FGF4, CD40, RETN) as well (CSF Aβ42) distinguish [area under curve (AUC) = 0.75 (plasma proteins), AUC 0.60 proteins) 0.56 Aβ42)]. Also, these performed better than line with Aβ42 differentiating CN versus subjects [AUC 0.89 0.85 These results adjusted age, sex, education, APOE ϵ4 genotype. Conclusions: suggests combination 6 can serve an effective marker CN.

Language: Английский

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Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 8, 2024

Abstract Endometriosis is a common cause of endometrial-type mucosa outside the uterine cavity with symptoms such as painful periods, chronic pelvic pain, pain intercourse and infertility. However, early diagnosis endometriosis still restricted. The purpose this investigation to identify validate key biomarkers endometriosis. Next generation sequencing (NGS) dataset GSE243039 was obtained from Gene Expression Omnibus (GEO) database, differentially expressed genes (DEGs) between normal control samples were identified. After screening DEGs, gene ontology (GO) REACTOME pathway enrichment analyses performed. Furthermore, protein-protein interaction (PPI) network constructed modules analysed using Human Integrated Protein-Protein Interaction rEference (HIPIE) database Cytoscape software, hub Subsequantely, miRNAs genes, TFss miRNet NetworkAnalyst tool, possible TFs predicted. Finally, receiver operating characteristic curve (ROC) analysis used genes. A total 958 including 479 up regulated down screened samples. GO DEGs showed that they mainly involved in multicellular organismal process, developmental signaling by GPCR muscle contraction. Further PPI identified 10 VCAM1, SNCA, PRKCB, ADRB2, FOXQ1, MDFI, ACTBL2, PRKD1, DAPK1 ACTC1. Possible target miRNAs, hsa-mir-3143 hsa-mir-2110, TFs, TCF3 CLOCK, predicted constructing miRNA-hub regulatory TF-hub network. This bioinformatics techniques explore potential novel biomarkers. These might provide new ideas methods for diagnosis, treatment, monitoring

Language: Английский

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Recipient Pericardial Apolipoprotein Levels Might Be an Indicator of Worse Outcomes after Orthotopic Heart Transplantation

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1752 - 1752

Published: Feb. 1, 2024

End-stage heart failure (ESHF) leads to hypoperfusion and edema formation throughout the body is accompanied by neurohormonal immunological alterations. Orthotopic transplantation (HTX) has been used as a beneficial option for ESHF. Due shortage of donor hearts, ideal matching timing donors recipients become more important. In this study, our aim was explore relationship between clinical outcomes HTX cytokine apolipoprotein profiles recipient pericardial fluid obtained at after opening sac. The data interleukin, adipokine, lipoprotein levels in twenty were investigated. Outcome variables included primer graft dysfunction (PGD), need post-transplantation mechanical cardiac support (MCS), International Society Heart Lung Transplantation grade ≥2R rejection, mortality. Recipient risk scores also Leptin significantly lower patients with PGD than those without (median: 6.36 (IQR: 5.55-6.62) versus 7.54 (IQR = 6.71-10.44); p 0.029). Higher ApoCII 14.91 11.55-21.30) 10.31 10.02-13.07); 0.042) ApoCIII 60.32 43.00-81.66) 22.84 15.84-33.39); 0.005) found (n 5) who died first 5 years HTX. exhibited rejection 4) month transplantation, adiponectin 74.48 35.51-131.70) 29.96 19.86-42.28); 0.039), 20.11 13.06-23.54) 10.32 10.02-12.84); 0.007), 70.97 34.72-82.22) 26.33 17.18-40.17); 0.029) higher nonrejection group. Moreover, thyroxine (T4) 3.96 3.49-4.46) 4.69 4.23-5.77); 0.022) did not develop rejection. Our results indicate that apolipoproteins can facilitate monitoring could be useful tool forecasting early late complications.

Language: Английский

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The potential protective role of Parkinson’s disease against hypothyroidism: co-localisation and bidirectional Mendelian randomization study

Frontiers in Aging Neuroscience, Journal Year: 2024, Volume and Issue: 16

Published: May 14, 2024

The association between hypothyroidism and Parkinson's disease (PD) has sparked intense debate in the medical community due to conflicting study results. A better understanding of this is crucial because its potential implications for both pathogenesis treatment strategies.

Language: Английский

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A disulfidptosis-related classification and risk signature identifies immunotherapy biomarkers and predicts prognosis in gastric cancer: An observational study

Medicine, Journal Year: 2024, Volume and Issue: 103(22), P. e38398 - e38398

Published: May 31, 2024

Gastric cancer (GC) is one of the most prevalent types globally, often detected at advanced stages. However, its prognosis remains poor, necessitating exploration new biomarkers. Disulfidptosis, a recently identified form programmed cell death, has not yet been investigated in relation to GC and associated mechanisms. We analyzed potential associations between disulfidptosis genes clinical risk using TCGA (The Cancer Genome Atlas)-STAD (stomach adenocarcinoma) as training set GSE84433 validation set. In addition, we explored prognostic value biological mechanisms disulfide by consensus clustering, enrichment analysis, mutation histology analysis immune infiltration analysis. Finally, constructed disulfidptosis-related signature (DRRS) assess association class, survival prognosis, infiltration. By utilizing data from 19 genes, successfully subgroups C1 C2 patients through clustering. Notably, 2 groups exhibited significant variations terms rates, scores, Subsequently, developed DRRS via LASSO (least absolute shrinkage selection operator) regression incorporating PRICKLE1, NRP1, APOD, MISP3, SERPINE1. This scoring system effectively distinguished individuals with high low risks, verified These findings strongly indicate close microenvironment tumors. Moreover, demonstrated commendable predictive capabilities for outcomes patients. this study, have different subtypes alterations immunotumour microenvironment. Furthermore, accuracy DRRS, valuable tool predicting survival, function, GC. contribute better comprehension offer opportunities innovative approaches treatment.

Language: Английский

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Impact of senescence in impaired wound healing

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 89 - 115

Published: Jan. 1, 2024

Language: Английский

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Identification of Serum Biomarkers and Therapeutic Targets for Aortic Diseases in Obesity through Multi-Omics Analysis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 28, 2024

Purpose Obesity is associated with an increased risk of aortic diseases and operative risks. Currently, there are no effective drugs available to prevent the occurrence progression aneurysms or dissections. We investigated potential biomarkers therapeutic targets using a multi-omics approach. Methods Clinical data from 117 patients disease were analyzed based on body mass index (BMI) explore relationship between BMI clinical outcomes. An obesity mouse model was developed by feeding high-cholesterol, high-fat diet (HCHFD), established administering human angiotensin II (AngII) at dose 1 mg/kg/day through osmotic minipumps. Through analysis murine transcriptomics serum proteomics, we identified for in obesity. Enzyme-linked immunosorbent assay used detect these serum. Results The duration hospitalization post-surgery positively correlated BMI. Transcriptomic revealed enrichment genes related complement coagulation cascades, as well prion pathway. Proteomic showed proteins African trypanosomiasis estrogen signaling By integrating transcriptomic protein profiles, C5 apoD adverse effects Conclusion High disease, especially dissection. Serum assessing obese individuals. Further research needed pathophysiological pathways linked their applications.

Language: Английский

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