In Vitro Determination of Antimicrobial, Antioxidant and Antiviral Properties of Greek Plant Extracts

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 177 - 177

Published: Jan. 16, 2025

The medicinal potential of plant extracts, especially their antimicrobial, antioxidant, antiviral and cytotoxic properties, has gained significant attention in recent years. This study examined the vitro bioactivities several selected Greek plants, like Eucalyptus globulus L., Thymus vulgaris Salvia rosmarinus L. Ocimum basilicum are well-known for traditional therapeutic use. Minimum inhibitory concentration (MIC) assays were used to evaluate antimicrobial activity extracts against pathogenic bacteria. antioxidant was carried out using DPPH method, while cytotoxicity plants determined Alamar Blue method. In addition, efficacy samples tested DENV different cell lines. majority herbs demonstrated action (MIC = 30–3000 μg∙mL−1). showed great activity, extract turned be most effective (IC50 12.89 ± 0.11 contrast, had lowest 71.02 0.42 results method presented with CC50 values, it shown that exhibited highest (CC50 5.94% v/v 0.04). Similarly, expressed as EC50 characterized sample dengue virus infection, values estimated at 2.37% 0.6 (HuhD-2 cells infected DENV-2) 0.36% 0.004 (Huh7.5 DVR2A). These findings provide a foundation further studies order combat infectious diseases promote human health.

Language: Английский

---

Hepatoprotective and Fat-Accumulation-Reductive Effects of Curcumin on Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 159 - 159

Published: Feb. 27, 2025

Fat accumulation is the hallmark of metabolic dysfunction-associated steatotic liver disease (MASLD). Given intimidating nature its treatment, curcumin (CUR) emerges as a potential therapeutic agent due to proven effectiveness in managing MASLD. This review aimed evaluate previous reports on hepatoprotective and fat-accumulation-reductive effects CUR administration preventing or treating can modulate serum enzymes lipid profiles. The fat MASLD primary cause oxidative stress inflammation. By reducing accumulation, may attenuate inflammation In addition, has been restore dysfunctional cellular energy metabolism capacity fibrogenesis (antifibrotic agent). Their are associated with Lipid (lipogenesis, lipolysis, lipophagy) correlated their effects. prophylactic effects, particularly early-stage MASLD, primarily when it used reducer. It be considered an excellent natural drug for because protects attenuates especially early stage development.

Language: Английский

---

Oxidative stress in a cellular model of alcohol-related liver disease: protection using curcumin nanoformulations

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 5, 2025

Alcohol-related liver disease (ARLD) is a global health issue causing significant morbidity and mortality, due to lack of suitable therapeutic options. ARLD induces spectrum biochemical cellular alterations, including chronic oxidative stress, mitochondrial dysfunction, cell death, resulting in hepatic injury. Natural antioxidant compounds such as curcumin have generated interest their ability scavenge reactive oxygen species (ROS), however, therapy using these limited poor bioavailability stability. Therefore, the aim this study was assess potential free antioxidants entrapped formulations against damage an model. HepG2 (VL-17A) cells were treated with varying concentrations alcohol (from 200 350 mM) parameters stress function assessed over 72 h. Data indicated mM ethanol led decrease viability at h, increase ROS 30 min. A substantial number late apoptosis reduction membrane also found. Pre-treatment nanoformulations increased viability, well as, reducing 2 48 h In summary, able ameliorate reduced caused by treatment. This demonstrates mitigating warrants further investigation evaluate efficacy for therapy.

Language: Английский

---

Molecular Mechanisms of Curcumin in the Pathogenesis of Metabolic Dysfunction Associated Steatotic Liver Disease

Nutrients, Journal Year: 2023, Volume and Issue: 15(24), P. 5053 - 5053

Published: Dec. 9, 2023

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a multifactorial condition characterized by insulin resistance, oxidative stress, chronic low-grade inflammation, and sometimes fibrosis. To date, no effective pharmacological therapy has been approved for the treatment of metabolic-associated steatohepatitis (MASH), progressive form MASLD. Recently, numerous in vitro vivo studies have described efficacy nutraceutical compounds diet tested. Among them, curcumin most widely used polyphenol showing potent anti-inflammatory antifibrotic activities. This review aims to summarize important basic (in animal models studies), describing molecular mechanisms which acts context MASLD, providing rationale its translational use humans.

Language: Английский

---

Silybin Alleviated Hepatic Injury by Regulating Redox Balance, Inflammatory Response, and Mitochondrial Function in Weaned Piglets under Paraquat-Induced Oxidative Stress

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 324 - 324

Published: March 6, 2024

Silybin (Si) is the main element of silymarin isolated from seeds Silybum marianum L. Gaernt., which has superior antioxidant properties. However, protective role Si in maintaining liver health under oxidative stress remains ambiguous. This study aimed to investigate underlying mechanism beneficial effect dietary against hepatic injury induced by paraquat (PQ) weaned piglets. A total 24 piglets were randomly allocated four treatments with six replicates per treatment and 1 piglet replicate: control group; PQ + group. Piglets group given a basal diet, while groups Si-supplemented diet. On 18th day, pigs received an intraperitoneal injection PQ, others intraperitoneally injected same volume saline. All sacrificed on day 21 for plasma sample collection. The results showed that supplementation mitigated PQ-induced damage, as proven reduction pathological changes activity alanine transaminase aspartate transaminase. also improved superoxide dismutase glutathione peroxidase activities capacity, well decreased malondialdehyde hydrogen peroxide concentration liver, closely related activation nuclear factor-erythroid 2-related factor 2 signaling pathway. Meanwhile, reduced tumor necrosis factor-α interleukin-8 production their transcript levels abrogated overactivation factor-κB PQ. Importantly, mitochondrial function energetics dynamics, was indicated elevated complexes I V adenosine triphosphate content, expression dynamin protein, increased mitofusin protein. Moreover, inhibited excessive apoptosis regulating B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated-X-protein Taken together, these potentially insults improving capacity inhibiting inflammation cell

Language: Английский

---

The Influence of Diet and Its Components on the Development and Prevention of Hepatocellular Carcinoma (HCC)

Cancers, Journal Year: 2024, Volume and Issue: 16(5), P. 1030 - 1030

Published: March 2, 2024

Hepatocellular carcinoma (HCC) is diagnosed annually in nearly a million people worldwide, with approximately half of them being at an advanced stage the disease. Non-infectious risk factors for development HCC include unbalanced lifestyle, including poor dietary choices characterized by low intake antioxidants, such as vitamins E and C, selenium, polyphenols, well excessive consumption energy harmful substances. Repeated bad that contribute to lifestyle lead accumulation fatty substances liver it entering inflammatory state, which, without intervention, results cirrhosis, main cause HCC. This review English language literature aims present food components that, when included daily diet, reduce developing HCC, identifying foods may have carcinogenic effect on cells.

Language: Английский

---

Relevant Aspects of Combined Protocols for Prevention of N(M)AFLD and Other Non‐Communicable Diseases

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: 68(7)

Published: March 20, 2024

Abstract Obesity is a global health issue characterized by the excessive fat accumulation, leading to an increased risk of chronic noncommunicable diseases (NCDs), including metabolic dysfunction‐associated fatty liver disease (MAFLD), which can progress from simple steatosis steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Currently, there are no approved pharmacological protocols for prevention/treatment MAFLD, due complexity lying beneath these mechanisms, monotherapies unlikely be efficacious. This review article analyzes possibility that NCDs prevented or attenuated combination bioactive substances, as they could promote higher response rates, maximum reaction results, additive synergistic effects compounds having similar different mechanisms action and/or refraining possible side effects, related use lower doses exposures times than monotherapies. Accordingly, prevention mouse MAFLD observed with omega‐3 docosahexaenoic acid antioxidant hydroxytyrosol, whereas attenuation mild cognitive impairment attained folic plus cobalamin in elderly patients. The existence several drawbacks underlying published combined trials, opens space adequate stricter experimental clinical tryouts achieve meaningful outcomes human applicability.

Language: Английский

---

The circadian rhythm: A new target of natural products that can protect against diseases of the metabolic system, cardiovascular system, and nervous system

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

The treatment of metabolic system, cardiovascular and nervous system diseases remains to be explored. In the internal environment organisms, metabolism substances such as carbohydrates, lipids proteins (including biohormones enzymes) exhibit a certain circadian rhythm maintain energy supply material cycle needed for normal activities organisms. As key factor health can disrupted by pathological conditions, this disruption accelerates progression results in vicious cycle. current treatments targeting have limitations, identification safer more effective regulators is needed. To systematically assess possibility using biological clock natural product target disease intervention, work reviews range evidence on potential effectiveness products protect against system. This manuscript focuses how restore function affecting amplitude expression factors, sleep/wake cycles structure gut microbiota. proposes that rhythm, which regulated rhythm-related factors cycle, crucial new slowing through use products. provides reference molecular modeling perspective time-targeted action drugs.

Language: Английский

---

Therapeutic Promises of Ferulic Acid and its Derivatives on Hepatic damage Related with Oxidative Stress and Inflammation: A Review with Mechanisms

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: 21(7)

Published: May 17, 2024

Ferulic acid (FA) is a naturally occurring phenolic compound commonly found in the plant Ferula communis. This study aims to investigate hepatoprotective effect of FA and its derivatives (methyl ferulic trans-ferulic acid) against oxidative stress inflammation-related hepatotoxicity due toxicants based on results different non-clinical preclinical tests. For this, data was collected from reliable electronic databases such as PubMed, Google Scholar, ScienceDirect, etc. The this investigation demonstrated that have potent effects damage. findings also revealed these protective are antioxidant anti-inflammatory chemical compound. analogues significantly inhibit free radical generation hinder proinflammatory markers inflammatory enzymes, resulting diminished cytotoxic apoptotic hepatocyte death. compounds prevent intracellular lipid accumulation provide effects.

Language: Английский

---

The Coming Age of Antisense Oligos for the Treatment of Hepatic Ischemia/Reperfusion (IRI) and Other Liver Disorders: Role of Oxidative Stress and Potential Antioxidant Effect

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 678 - 678

Published: May 31, 2024

Imbalances in the redox state of liver arise during metabolic processes, inflammatory injuries, and proliferative disorders. Acute exposure to intracellular reactive oxygen species (ROS) results from high levels oxidative stress (OxS) that occur response hepatic ischemia/reperfusion injury (IRI) diseases liver. Antisense oligonucleotides (ASOs) are an emerging class gene expression modulators target RNA molecules by Watson–Crick binding specificity, leading degradation, splicing modulation, and/or translation interference. Here, we review ASO inhibitor/activator strategies modulate transcription control enzymes, factors, sensors DNA damage. Several small-interfering (siRNA) drugs with N-acetyl galactosamine moieties for have recently been approved. Preclinical studies using short-activating RNAs (saRNAs), phosphorodiamidate morpholino oligomers (PMOs), locked nucleic acids (LNAs) at forefront proof-in-concept therapeutics. Future research targeting OxS-related pathways may help realize promise precision medicine, revolutionizing customary approach caring treating individuals afflicted liver-specific conditions.

Language: Английский

---